Journal
BIOELECTROCHEMISTRY
Volume 76, Issue 1-2, Pages 76-80Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2009.04.004
Keywords
Cytochrome c-based biosensor; Superoxide radicals; Antioxidant capacity; Orange juices; Screen-printed gold electrodes
Funding
- European Commission [FOOD-CT-2005-023044]
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This paper describes the development of an amperometric cytochrome c (cyt c)-based biosensor and its later application to the quantification of the scavenging capacity of antioxidants. The enzymatic biosensor was constructed by covalently co-immobilizing both cyt c and XOD on a mercaptoundecanol/mercaptoundecanoic acid (MU/MUA) mixed self-assembled monolayer (SAM)-modified screen-printed gold electrode. The applicability of this method was shown by analyzing the antioxidant capacity of pure substances, such as ascorbic acid and Trolox, and natural sources of antioxidants, particularly 5 orange juices. (C) 2009 Elsevier B.V. All rights reserved.
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