Article
Multidisciplinary Sciences
Ahmet C. Sabuncu, Sinan Muldur, Barbaros Cetin, O. Berk Usta, Nadine Aubry
Summary: This study experimentally examined the aggregation of human red blood cells using Hetastrach as an aggregation agent, measuring parameters such as impedance and sedimentation kinetics to distinguish different aggregation states in blood. Theoretical modeling was used to analyze the complex permittivity of red blood cell aggregates at various concentrations of Hetastrach, providing insights into the dynamics of aggregation and sedimentation processes.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Ivan T. Ivanov, Boyana K. Paarvanova
Summary: Dielectric relaxations were studied on the erythrocyte spectrin network using dielectric spectroscopy. The extraction of membrane lipids preserved γ1(sp) and removed β(sp)-relaxation. The concentration of NaCl and the presence of certain substances influenced the strength of β(sp)-relaxation and γ1(sp)-relaxation differently.
Article
Chemistry, Multidisciplinary
Chin-Li Lin, Jong-Shyan Wang, Tieh-Cheng Fu, Chih-Chin Hsu, Yu-Chieh Huang
Summary: The study elucidates the effects of hypoxic exercise training on intrinsic/extrinsic factors of erythrocyte aggregation, revealing that hypoxic training increases global aggregation of erythrocytes, while normoxic training is mainly associated with improved fibrinogen-erythrocyte interactions. In addition, hypoxic training promotes erythrocyte senescence.
APPLIED SCIENCES-BASEL
(2021)
Article
Multidisciplinary Sciences
Olivier L. Charansonney, Ghanima Al-Dandachi, Patrick Plaisance, Eric Vicaut
Summary: This study aimed to evaluate the effectiveness of a new point-of-care test, measuring erythrocyte aggregation kinetics (EAK), in identifying patients with underlying inflammation in emergency settings. The results showed that EAK is a better marker of inflammation compared to C-Reactive Protein (CRP), with higher specificity and sensitivity.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Michael Pycraft Hughes, Emily J. Kruchek, Andrew D. Beale, Stephen J. Kitcatt, Sara Qureshi, Zachary P. Trott, Oriane Charbonnel, Paul A. Agbaje, Erin A. Henslee, Robert A. Dorey, Rebecca Lewis, Fatima H. Labeed
Summary: The membrane potential V-m plays crucial roles in cell function, influencing extracellular potential and ion composition, which has significant implications for cellular interactions and disease development.
SCIENTIFIC REPORTS
(2021)
Article
Medical Laboratory Technology
Makoto Higuchi, Nobuo Watanabe
Summary: The determination of erythrocyte sedimentation rate (ESR) using erythrocyte aggregation measurement is an alternative to the Westergren method and can be performed quickly. However, the principle of this method is not clear, and the ESR values obtained may differ from those obtained using the Westergren method due to hematocrit. Additionally, particle size affects the Westergren ESR, but the effect of individual mean corpuscular volumes (MCVs) has not been studied.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2023)
Article
Hematology
Samantha Weber-Fishkin, Harrison S. Seidner, Geoffrey Gunter, Mary D. Frame
Summary: This study aims to investigate the kinetics of erythrocyte aggregation under different temperature and oxygen conditions. The results showed that erythrocytes aggregate faster and with a higher magnitude in hyperthermia and hypoxemia, especially in autologous plasma. Furthermore, the C-terminal domain of band 3 plays a pivotal role in erythrocyte aggregation.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Chemistry, Analytical
Yang Jun Kang
Summary: In this study, a simple method was proposed to sequentially quantify blood and diluent in microfluidic channels, reducing the blood volume from 1 mL to 50 μL. The method showed substantial differences in detecting aggregation-elevated blood compared to previous methods.
ANALYTICAL METHODS
(2022)
Article
Engineering, Biomedical
Jun Dai, Zhaojun Chen, Shixuan Wang, Fan Xia, Xiaoding Lou
Summary: In this study, erythrocyte membrane camouflaged nanoparticles (NPs) were developed and two photosensitizers were prepared using autologous and allogeneic erythrocytes. The results showed that both photosensitizers had similar therapeutic efficacy and biosafety in inhibiting tumor growth. They did not affect the general condition, organ function, or immune system of treated mice. The findings provide further evidence for the promising therapeutic platform of erythrocyte membrane camouflaged NPs in terms of efficacy and biosafety.
MATERIALS TODAY BIO
(2022)
Article
Multidisciplinary Sciences
Cheong-Ah Lee, Dong-Guk Paeng
Summary: The study utilized a 2D RBC model to simulate RBC motion under sinusoidal pulsatile flow, revealing the significant impact of axial shear rate and its ratio with radial shear rate on local RBC aggregation and parabolic rouleaux formation.
SCIENTIFIC REPORTS
(2021)
Article
Astronomy & Astrophysics
H. Jia, S. Y. Wang, B. Qi, C. Liu, L. Zhu
Summary: In this study, the mixing ratios of Delta the Delta I = 1 linking transitions in the chiral and multiple chiral doublet bands were analyzed for the first time using the quantum particle rotor model. The calculated results showed that the Delta I = 1 linking transitions in the lowest and excited chiral doublet bands were of pure M1 character, while those connecting the excited to lowest chiral doublet bands had an M1/E2 character with a relatively large E2 admixture. Further analysis of the angular momentum geometry and azimuthal plots revealed that the excited chiral doublet bands represented a chiral geometry with a wobbling motion of the core, providing a possible figure of wobbling motion in multiple chiral doublets.
Article
Optics
Hui Cheng, XinLin Chen, JinCheng Zhong, Jia Li, Ping Qiu, Ke Wang
Summary: In this study, we demonstrate a method for measuring instantaneous wall shear rate and WSS using dual-wavelength third-harmonic-generation (THG) line-scanning imaging. The oscillating behavior of WSS in brain venules and arterioles at micron spatial resolution is observed in a label-free manner.
Article
Biology
Cheong-Ah Lee, Hafiz Muhammad Umer Farooqi, Dong-Guk Paeng
Summary: Erythrocyte aggregation is an important phenomenon in interpreting human hemorheology for the diagnosis and prediction of circulatory anomalies. Previous studies have not considered the natural pulsatility of blood flow or large vessels, and mainly focused on shear rate to understand the dynamic properties of erythrocyte aggregation. The rheological characteristics of non-Newtonian fluids under Womersley flow have not reflected the spatiotemporal behaviors of erythrocyte dynamics. This study provides new insights into the effect of axial shear rate on erythrocyte aggregation and its distribution, which has implications for the diagnosis of cardiovascular diseases based on hemodynamics.
COMPUTERS IN BIOLOGY AND MEDICINE
(2023)
Article
Astronomy & Astrophysics
A. Bolanos-Carrera, R. Sanchez-Velez, G. Tavares-Velasco
Summary: In this article, the one-loop contribution to the rare three-body flavor changing neutral current top quark decay t -> c gamma gamma is calculated in the framework of models with scalar leptoquark doublets. Analytical expressions for the invariant amplitude of the decay fi -> fj gamma gamma are obtained in terms of Passarino-Veltman integral coefficients, which allow for the calculation of the amplitudes for t -> c gamma gamma and ei -> ej gamma gamma. The parameter space is constrained using current limits and bounds on the leptoquark couplings are derived. The branching ratio for the t -> c gamma gamma decay is estimated to be on the order of 10-11-10-12 for LQ masses in the range of 1-1.5 TeV, making it difficult to be observed in future experiments.
Article
Biochemical Research Methods
Leah L. Weber, Palash Sashittal, Mohammed El-Kebir
Summary: The study introduces a standalone method, doubletD, for detecting doublets in scDNA-seq data, achieving high performance through a simple maximum likelihood approach that outperforms current methods. Applying doubletD in scDNA-seq analysis pipelines can reduce complexity and improve result accuracy.
Article
Biophysics
Ana Hocevar Brezavscek, Matteo Rauzi, Maria Leptin, Primoz Ziherl
BIOPHYSICAL JOURNAL
(2012)
Review
Biophysics
Matteo Rauzi, Ana Hocevar Brezavscek, Primoz Ziherl, Maria Leptin
BIOPHYSICAL JOURNAL
(2013)
Article
Physics, Multidisciplinary
Ioannis A. Georgiou, Primoz Ziherl, Gerhard Kahl
Article
Chemistry, Physical
U. Jelercic, P. Ziherl
EUROPEAN PHYSICAL JOURNAL E
(2013)
Article
Multidisciplinary Sciences
T. Dotera, T. Oshiro, P. Ziherl
Article
Physics, Multidisciplinary
A. Siber, P. Ziherl
PHYSICAL REVIEW LETTERS
(2013)
Article
Chemistry, Physical
Ai Sakashita, Naohito Urakami, Primoz Ziherl, Masayuki Imai
Article
Chemistry, Physical
M. Krajnc, N. Storgel, A. Hocevar Brezavscek, P. Ziherl
Article
Multidisciplinary Sciences
Joichiro Nakakura, Primoz Ziherl, Junichi Matsuzawa, Tomonari Dotera
NATURE COMMUNICATIONS
(2019)
Article
Multidisciplinary Sciences
Jan Rozman, Matej Krajnc, Primoz Ziherl
NATURE COMMUNICATIONS
(2020)
Article
Chemistry, Physical
Jan Rozman, Matej Krajnc, Primoz Ziherl
Summary: Using a three-dimensional active vertex model, this study numerically investigates the shapes of strained unsupported epithelial monolayers subject to active junctional noise. The research reveals that different types of compressive strains lead to different fold morphologies, and villus morphology appears only when junctional tension fluctuations are strong enough to fluidize the tissue. Additionally, fluidized epithelium can form villi even in the absence of compressive strain under large apico-basal differential surface tension.
EUROPEAN PHYSICAL JOURNAL E
(2021)
Article
Physics, Multidisciplinary
Urska Andrensek, Primoz Ziherl, Matej Krajnc
Summary: We investigate the elasticity of an unsupported epithelial monolayer and discover that it may wrinkle even in the absence of the underlying substrate. We derive an exact elasticity theory from a cell-based model and find that wrinkling is driven by the differential apico-basal surface tension. By introducing a phantom substrate with finite stiffness beyond a critical tension, our theory is mapped onto that for supported plates, suggesting a new mechanism for tissues to control their surface patterns autonomously.
PHYSICAL REVIEW LETTERS
(2023)
Article
Chemistry, Physical
Toshikaze Chiba, Yuka Sakuma, Masayuki Imai, Primoz Ziherl
Summary: We studied the morphological changes of adhering vesicle triplets under different adhesion regimes and analyzed their dependence on volume-to-area ratio. In the strong adhesion regime, the triplet morphology varied based on the arrangement of vesicles. Decreasing the volume-to-area ratio transformed a triangular triplet into a compact spherical shape with a sigmoidal contact zone, while a linear vesicle triplet deformed into a compact spherical shape with biconvex interfaces. The morphologies of triplets with flat contact zones were explained by the two-tension model, but when the interface deformed, the cavity model was more appropriate. In the weak adhesion regime, both triangular and linear vesicle triplets transformed into prolate-based triplets by reducing the volume-to-area ratio.
Article
Physics, Fluids & Plasmas
M. Kokalj Ladan, P. Ziherl, A. Siber
Article
Chemistry, Physical
B. Kavcic, A. Sakashita, H. Noguchi, P. Ziherl
Article
Biochemistry & Molecular Biology
Priyanga Kumar, Noel Nesakumar, Jayashree Gopal, Sakthivel Sivasubramanian, Srinivasan Vedantham, John Bosco Balaguru Rayappan
Summary: This study developed an electrochemical biosensor for measuring MG levels in the plasma of type-2 diabetes mellitus patients. The biosensor showed a wide linear concentration range, high sensitivity, and a 90% correlation with ELISA data. It also exhibited a significant correlation with HbA1c and fasting plasma glucose levels.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Cristina Tortolini, Valeria Gigli, Antonio Angeloni, Federico Tasca, Nguyen T. K. Thanh, Riccarda Antiochia
Summary: The development of a novel voltammetric immunosensor for the detection of salivary MMP-8 at the point-of-care is described. The sensor showed good performance and comparable results to the conventional ELISA method when tested in real saliva samples. This biosensor is single-use, cost-effective, and requires a small quantity of test medium and a short preparation time.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Rimsha B. Jamal, Ulrich Bay Gosewinkel, Elena E. Ferapontova
Summary: Pathogen-triggered infections are a severe global threat to human health. Researchers have developed a fast and inexpensive electrocatalytic aptamer assay for the specific and ultrasensitive detection of E. coli, allowing for timely treatment and prevention. The method is fast, sensitive, and can be used in field and point-of-care applications for analysis of bacteria in the human environment.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Miao Liu, Yuwei Wang, Shanshan Tang, Wei Wang, Axin Liang, Aiqin Luo
Summary: An unlabeled ratiometric molecular imprinted electrochemiluminescence sensor was developed for the determination of trace uric acid using MXene@NaAsc, CdSe@ZnS quantum dots, and molecularly imprinted polymer composites modified glass carbon electrode. This sensor, with easy preparation, great selectivity, and excellent sensitivity, successfully detected uric acid in human serum.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Charlotte Da Silva, Camille Lamarche, Carole Pichereaux, Emmanuelle Mouton-Barbosa, Gauthier Demol, Sebastien Boisne, Etienne Dague, Odile Burlet-Schiltz, Flavien Pillet, Marie-Pierre Rols
Summary: Low-energy electron beams (LEEB) are a safe and practical sterilization solution for industrial applications. To address the limitations of LEEB, we developed a low-energy pulsed electron beam generator (LEPEB) that can effectively and efficiently eradicate bacteria in a wide range of industrial applications.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Daniel Dobrovodsky, Ales Danhel, Daniel Renciuk, Jean-Louis Mergny, Miroslav Fojta
Summary: In this study, N-methyl mesoporphyrin IX (NMM) was utilized as a voltammetric probe for the electrochemical detection of G4s. The detection of NMM was achieved by cyclic voltammetry on a hanging mercury drop electrode (HMDE) with a limit of detection (LOD) of 40 nM. The reduction signal of NMM was found to be significantly higher when G4 oligodeoxynucleotides (G4 ODNs) were present compared to single- or double-stranded ODNs or unfolded ODNs capable of forming G4s. Gradual transition from unstructured single strand to G4, induced by increasing concentrations of the G4 stabilizing K+ ions, was detected for the first time using electrochemical methods. Circular dichroism spectroscopy provided support for the obtained results. This work expands on the utilization of electrochemical probes for DNA secondary structure recognition and offers a proof of principle for the development of novel electroanalytical methods for nucleic acid structure studies.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Mengjiao Zhu, Yu Liu, Meiyue Wang, Tao Liu, Zhenyu Chu, Wanqin Jin
Summary: Early rapid diagnosis of COVID-19 is crucial for reducing the risk of severe symptoms and loss of lung function. Researchers have proposed an ultrafast and ultrasensitive DNA sensor that can accurately detect the virus in a short period of time, with outstanding selectivity.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Fatima-Zahra Ait-Itto, James A. Behan, Mathieu Martinez, Frederic Barriere
Summary: This study investigated the feasibility of using ancient marine sediments as inoculum for bioanode development in microbial fuel cells (MFC). The results showed the presence of two exoelectrogenic bacterial genera in these iron-rich sediments and confirmed that the development of the bioanode derived from the native microbiota. This study has important implications for understanding the role of these bacteria in broader paleoenvironmental phenomena.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Issa Fall, Bastien Doumeche, Sofiene Abdellaoui, Caroline Remond, Harivony Rakotoarivonina, Marjorie Ochs
Summary: This article presents a novel electrochemical tool based on lignin-coated paper electrodes for the detection and characterization of ligninolytic activity. The suitability of this method has been demonstrated using a catalaseperoxidase isolated from Thermobacillus xylanilyticus.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Chenyi Zhuo, Dehong Yu, Jiuying Cui, Zichun Song, Qianli Tang, Xianjiu Liao, Zhao Liu, Ning Xin, Lu Lou, Fenglei Gao
Summary: A label-free detection strategy for quantification of Apolipoprotein A4 (Apo-A4) has been developed based on rolling circle amplification (RCA) and proximity hybridization-induced DNAzyme-driven bipedal DNA walker. This method shows high sensitivity and selectivity for a wide range of protein targets.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Niloufar Fattahi, Jeffrey Reed, Evan Heronemus, Priyasha Fernando, Ryan Hansen, Prathap Parameswaran
Summary: In this study, polyethylene glycol hydrogels were developed as protective coatings for electroactive biofilms, improving their viability under low resource conditions and ammonia-N shocks.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Xiaopeng Hou, Qiongyi Cheng, Hui Wang
Summary: A self-cleaning paper-based microfluidic biosensor was developed using hydrophobic paper decorated with polymeric octadecyl trichlorosilane. The biosensor showed improved performance and could effectively measure the concentration of copper ions in livestock feed and manure.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Yanling Zhang, Jie Wang, Min Liu, Yun Ni, Yao Yue, Dawei He, Ruijiang Liu
Summary: An innovative electrochemical biosensor has been developed for sensitive detection of HER2 protein, an important tumor marker for breast cancer diagnosis and treatment evaluation. The biosensor utilizes specific recognition probes and nanomaterials, along with a unique sensing strategy, to achieve ultra-low detection limit and demonstrate excellent selectivity, reproducibility, and stability.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Humayun Amir, Vasanth Subramanian, Sundaram Sornambikai, Nagamony Ponpandian, Chinnusamy Viswanathan
Summary: In this study, the electrochemical detection of breast cancer biomarker HER2 was successfully achieved using the N-CQDs/GS as the detector. The BSA-modified HER2 Antibody/N-CQDs/GS immunoelectrode preserved excellent activity for the biosensor, while the GS electrode provided high stability and conductivity. The method exhibited a low detection limit and optimal specificity, stability, and reproducibility for detecting HER-2 protein in untreated blood samples from breast cancer patients.
BIOELECTROCHEMISTRY
(2024)
Article
Biochemistry & Molecular Biology
Ilona Uzieliene, Anton Popov, Raminta Vaiciuleviciute, Gailute Kirdaite, Eiva Bernotiene, Almira Ramanaviciene
Summary: This review discusses the application of polypyrrole (Ppy), an electroconductive polymer, in cell culture experiments with electrical stimulation (ES) and its potential for tissue regeneration. Ppy can direct ES to cells and stimulate differentiation towards different cell lineages. Different types of Ppy materials and their combination with active molecules are explored.
BIOELECTROCHEMISTRY
(2024)