Journal
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
Volume 119, Issue 4, Pages 492-495Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1016/j.jbiosc.2014.09.018
Keywords
Monodisperse microdroplets; Microfluidic device; Centrifuge; Plateau-Rayleigh instability; Biochemical reactions; Cell encapsulation
Funding
- PRESTO Design and Control of Cellular Functions research area of Japan Science and Technology Agency (JST)
- Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan [24104002]
- Japan Society for the Promotion of Science (JSPS) [24680033, 26280097]
- Creative Design for Bioscience and Biotechnology course of the School of Bioscience and Biotechnology at Tokyo Tech
- Grants-in-Aid for Scientific Research [14J10002, 26280097, 24104002, 24680033] Funding Source: KAKEN
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We report an easy-to-use generation method of biologically compatible monodisperse water-in-oil microdroplets using a glass-capillary-based microfluidic device in a tabletop mini-centrifuge. This device does not require complicated microfabrication; furthermore, only a small sample volume is required in experiments. Therefore, we believe that this method will assist biochemical and cell-biological experiments. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.
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