Journal
BIOCONTROL SCIENCE AND TECHNOLOGY
Volume 24, Issue 1, Pages 90-102Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09583157.2013.847905
Keywords
glucanase; antifungal activity; Streptomyces lydicus; natamycin production
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Funding
- Science and Technology Plan Project of Beijing [Z121100001212002]
- Special Research Fund for Agro-Scientific Research in the Public Interest from the Chinese Agriculture Ministry [200903049-07]
- Science Research Innovation Fund from Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences [CCJJZDXM201201-2]
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Streptomyces have been used extensively as the biocontrol agents due to their ability to produce various antimicrobial compounds, such as antibiotics and hydrolytic enzymes. Streptomyces lydicus strain A02, which was isolated from the soil of suburban forest field in Beijing (China), is capable of producing natamycin and has proved to be a potential biocontrol agent to several plant fungal diseases, including wilts caused by Fusarium oxysporum f. spp. However, hydrolytic enzymes like glucanase have not been detected in S. lydicus A02 on CMC-Na plates by congo red staining. Glucanase, a pathogenesis-related (PR) protein, degrades fungal cell walls and has been widely used as antifungal agent in plant protection. Therefore, a recombinant S. lydicus expressing a glucanase gene, which was cloned from the biocontrol strain Bacillus megaterium L103 and driven by the Streptomyces erythraea ermE* promoter, was constructed in this study. The engineered S. lydicus AG02 shared a similar yield of natamycin with the wild-type A02 strain. Compared to the wild-type strain A02, the engineered S. lydicus AG02 had a remarkably higher glucanase activity, as well as antifungal activity to F. oxysporum f. sp. conglutinans, F. oxysporum f. sp. niveum and Rhizoctonia cerealis. This demonstrated the improved biocontrol effect of S. lydicus AG02 attributed to transforming the exogenous glucanase from B. megaterium, which acted synergistically with natamycin to increase the antifungal activity of the strain.
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