Journal
JOURNAL OF BIOPHOTONICS
Volume 8, Issue 11-12, Pages 884-888Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.201400116
Keywords
polarimetric microscopy; multiphoton microscopy; neurophotonic; neuroscience
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Funding
- NSERC
- Canada Research Chair Program
- CIHR Emerging Team Program
- MS Society of Canada
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Polarimetric measurements in multiphoton microscopy can reveal information about the local molecular order of a sample. However, the presence of a dichroic through which the excitation beam propagates will generally scramble its polarization. We propose a simple scheme whereby a second properly-oriented compensation dichroic is used to negate any alteration regardless of the wavelength and the initial polarization. We demonstrate how this robust and rapid approach simplifies polarimetric measurements in second-harmonic generation, two-photon excited fluorescence and coherent anti-Stokes Raman scattering. Illustration of the polarization maintaining strategy with the compensating dichroic oriented such that its s- and p-axes are interchanged with these of the primary dichroic.
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