Journal
JOURNAL OF BIOPHOTONICS
Volume 8, Issue 10, Pages 855-863Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.201400134
Keywords
surface-enhanced fluorescence; nanoporous gold disks; label-free biosensor; zeptomole detection; molecular beacon
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Funding
- National Science Foundation (NSF) CAREER Award [CBET-1151154]
- National Aeronautics and Space Administration (NASA) Early Career Faculty Grant [NNX12AQ44G]
- Gulf of Mexico Research Initiative [GoMRI-030]
- Cullen College of Engineering at University of Houston
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We experimentally demonstrate a label-free biosensor for the ERBB2 cancer gene DNA target based on the distance-dependent detection of surface-enhanced fluorescence (SEF) on nanoporous gold disk (NPGD) plasmonic nanoparticles. We achieve detection of 2.4 zeptomole of DNA target on the NPGD substrate with an upper concentration detection limit of 1 nM. Without the use of molecular spacers, the NPGD substrate as an SEF platform was shown to provide higher net fluorescence for visible and NIR fluorophores compared to glass and non-porous gold substrates. The enhanced fluorescence signals in patterned nanoporous gold nanoparticles make NPGD a viable material for further reducing detection limits for biomolecular targets used in clinical assays.
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