Journal
JOURNAL OF BIOPHOTONICS
Volume 9, Issue 9, Pages 942-947Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/jbio.201500240
Keywords
femtosecond laser; induced pluripotent stem cells; reprogramming; optical transfection; flow cell
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Funding
- German Science Foundation (DFG) [SPP1327]
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Induced pluripotent stem cell (iPS cell) technology can be used to produce unlimited numbers of functional cells for both research and therapeutic purposes without ethical controversy. Typically, viruses are applied for efficient intracellular delivery of genes/transcription factors to generate iPS cells. However, the viral genomic integration may cause a risk of mutation as well as tumor formation therefore limits its clinical application. Here we demonstrate that spatially shaped extreme ultrashort laser pulses of sub-20 femtoseconds induce transient membrane permeabilisation which enables contamination-free transfection of cells in a microfluidic tube with multiple genes at the individual cell level in order to achieve optical reprogramming of large cell populations. We found that the ultrashort femtosecond laser-microfluidic cell transfection platform enhanced the efficacy of iPS-like colony-forming following merely a single transfection. [GRAPHICS] Illustration of the spatially shaped femtosecond laser-assisted microfluidic cell transfection platform for production of iPS cell colonies.
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