Article
Biochemistry & Molecular Biology
Giulia Bertacchi, Wilfried Posch, Doris Wilflingseder
Summary: Immune cells communicate through tunneling nanotubes (TNTs), and HIV-1 can spread through these TNTs. This study found that when HIV-1 is complement-opsonized, dendritic cells (DCs) can be efficiently infected, leading to improved antiviral and T-cell stimulatory capacity. The role of TNTs and complement in enhancing DC infection with complement-opsonized HIV-1 was investigated.
Article
Chemistry, Medicinal
Ladislav Prener, Ondrej Baszczynski, Martin M. Kaiser, Martin Dracinsky, George Stepan, Yu-Jen Lee, Boris Brumshtein, Helen Yu, Petr Jansa, Eric B. Lansdon, Zlatko Janeba
Summary: Novel bicyclic NNRTIs with improved solubility and high antiviral activity against HIV-1 were designed and prepared. The binding modes of these compounds to reverse transcriptase were also studied by X-ray crystallography.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Review
Immunology
Jeffrey C. Umotoy, Steven W. de Taeye
Summary: Despite the improvements in life expectancy for people living with HIV-1 through antiretroviral therapy, a cure for HIV/AIDS remains elusive. The emergence of drug resistance has led to increased treatment failures, highlighting the need for novel strategies for HIV-1 cure. Antibody conjugates (ACs) show promise in addressing limitations of current ART, reducing off-target effects, toxicity, and production costs, and are being explored for their potential in HIV-1 cure.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Seung-Wan Yoo, Abdul A. Waheed, Pragney Deme, Sehmus Tohumeken, Rana Rais, Matthew D. Smith, Catherine DeMarino, Peter A. Calabresi, Fatah Kashanchi, Eric O. Freed, Barbara S. Slusher, Norman J. Haughey
Summary: This study found that neutral sphingomyelinase 2 (nSMase2) interacts with HIV-1 Gag, and through the hydrolysis of sphingomyelin creates ceramide that plays a critical role in the formation of the viral envelope and maturation. Inhibition or depletion of nSMase2 leads to the production of noninfectious HIV-1 virions with incomplete Gag lattices. In vivo and in vitro experiments suggest that nSMase2 could be a potential therapeutic target for killing HIV-1-infected cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Immunology
Shawn Abeynaike, Silke Paust
Summary: While HIV-1 infection can now be managed with lifelong treatment, complete eradication remains a challenge due to the virus's ability to remain in dormant state in tissue reservoirs and its high mutation rate. Developing a suitable animal model is critical for evaluating pathogenesis and therapeutic approaches for HIV-1 cure strategies.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Guanghui Zong, Christian Toonstra, Qiang Yang, Roushu Zhang, Lai-Xi Wang
Summary: The study synthesized HIV-1 V1V2 glycopeptide-Q beta conjugates as a mimic of the proposed neutralizing epitope of PG9, showing significantly enhanced affinity for the PG9 antibody, depending on the density of the glycopeptide antigen display. These conjugates represent a promising candidate for an HIV-1 vaccine.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Medicinal
Yan Liu, Jiaqing Huang, Min Wu, Bi Liu, Qiaofa Lin, Jingjing Wu, Yuhua Ouyang, Xin Guo, Ruyi Huang, Yongmin Zhang, Jianhua Xu
Summary: Six positional isomers of triptolide-glucose conjugates (TG1 alpha, TG1 beta, TG2, TG3, TG4 and TG6) were synthesized and showed improved water solubility. Among them, TG2, formed by triptolide and D-glucose, exhibited the strongest cytotoxicity to tumor cells and the lowest toxicity to non-tumor cells, resulting in the highest relative therapeutic index and the most powerful selective antitumor activity in vitro.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
George Leonidis, Anastasia Koukiali, Ioanna Sigala, Katerina Tsimaratou, Dimitris Beis, Thomas Giannakouros, Eleni Nikolakaki, Vasiliki Sarli
Summary: This study presents the optimized synthesis of JH-VII-139-1 and its c(RGDyK) peptide conjugates, and investigates their chemostability, cytotoxic and antiangiogenic properties. Most conjugates retained mid nanomolar-level inhibitory activity against SRPK1 kinase, and two conjugates showed antiproliferative effects against multiple cancer cells. In vivo zebrafish screening assays demonstrated the ability of the synthesized conjugates to inhibit intersegmental vessels. Additionally, flow cytometry experiments revealed receptor-mediated endocytosis of the conjugates in specific cancer cells.
Article
Microbiology
Abigail Gerberick, Diana C. DeLucia, Paolo Piazza, Mounia Alaoui-El-Azher, Charles R. Rinaldo, Nicolas Sluis-Cremer, Giovanna Rappocciolo
Summary: The research found that B lymphocytes have the unique ability to efficiently trans infect T-N in vitro, and T-N isolated from nonprogressors (NP) harbor significantly lower levels of HIV-1 DNA, indicating that B cell-mediated trans infection of T-N with HIV-1 plays a more profound role than previously considered in establishing the viral reservoir and controlling HIV-1 disease progression.
Article
Polymer Science
Kaixuan Dong, Junying Zhang, Aihua He
Summary: Polymer chain-end functionalization with polar groups can enhance the hydrophilicity of polymers. In this study, a facile synthesis strategy for amine-functionalized trans-1,4-polyisoprenes was proposed using DCHA as a chain-transfer agent with a Ziegler-Natta catalyst. The F-TPI samples were separated into low-molecular-weight and high-molecular-weight fractions with different capping efficiencies, showing improved hydrophilicity with increased CE.
Article
Microbiology
Wanying Zhang, Mo Zhou, Cancan Chen, Shiyu Wu, Lilin Wang, Baijin Xia, Jun Liu, Xiancai Ma, Ting Pan, Hui Zhang, Linghua Li, Bingfeng Liu
Summary: In this study, CD98 was identified as a novel plasma membrane biomarker for HIV-1 permissiveness and latent infection. Importantly, CD98(high) CD4(+) T cells exhibited a hyper-permissiveness to HIV-1 infection and significantly contributed to the clonal expansion of the HIV-1 latent reservoir. CD98 could be targeted for further research to develop therapeutic strategies to reduce the HIV-1 latent reservoir.
Review
Immunology
Xi Liu, Yuyun Jiang, Jixian Ye, Xuefeng Wang
Summary: The pathogenesis of NAFLD involves abnormal glycolipid metabolism, inflammation, and gut dysbiosis. Helminths and their products can treat or relieve NAFLD-related diseases by promoting glycolipid metabolism homeostasis, regulating inflammation, and restoring the balance of gut microbiota.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Paula Costales, Nicolas Rios-Lombardia, Seila Lorenzo-Herrero, Francisco Moris, Javier Gonzalez-Sabin
Summary: A novel series of enantiopure naphthalimide-cycloalkanediamine conjugates were designed, synthesized and evaluated for their in vitro cytotoxicity. The cytotoxicity of the compounds depended on the size of the cycloalkyl ring and length of the spacer, with no observed enantioselectivity. Compounds 22 and 23 were found to effectively induce G2/M arrest in the tested cell lines, despite a mild apoptotic effect.
BIOORGANIC CHEMISTRY
(2021)
Review
Microbiology
Daniel Perez-Zsolt, Dalia Raich-Regue, Jordana Munoz-Basagoiti, Carmen Aguilar-Gurrieri, Bonaventura Clotet, Julia Blanco, Nuria Izquierdo-Useros
Summary: Cell-to-cell transmission of HIV-1 is a highly infectious mechanism that utilizes dendritic cells and lectin receptor Siglec-1 to transfer viral particles. This mechanism can induce immune responses, enhance cellular infection, or promote innate sensing, leading to viral transmission.
Article
Medicine, General & Internal
Sorana Segal-Maurer, Edwin DeJesus, Hans-Jurgen Stellbrink, Antonella Castagna, Gary J. Richmond, Gary I. Sinclair, Krittaecho Siripassorn, Peter J. Ruane, Mezgebe Berhe, Hui Wang, Nicolas A. Margot, Hadas Dvory-Sobol, Robert H. Hyland, Diana M. Brainard, Martin S. Rhee, Jared M. Baeten, Jean-Michel Molina
Summary: Patients with multidrug-resistant HIV-1 infection showed greater reduction in viral load when treated with lenacapavir compared to placebo.
NEW ENGLAND JOURNAL OF MEDICINE
(2022)
Article
Chemistry, Multidisciplinary
Charlotte Sornay, Steve Hessmann, Stephane Erb, Igor Dovgan, Anthony Ehkirch, Thomas Botzanowski, Sarah Cianferani, Alain Wagner, Guilhem Chaubet
CHEMISTRY-A EUROPEAN JOURNAL
(2020)
Article
Oncology
Caroline Spenle, Thomas Loustau, Devadarssen Murdamoothoo, William Erne, Stephanie Beghelli-de la Forest Divonne, Romain Veber, Luciana Petti, Pierre Bourdely, Matthias Morgelin, Eva-Maria Brauchle, Gerard Cremel, Vony Randrianarisoa, Abdouramane Camara, Samah Rekima, Sebastian Schaub, Kelly Nouhen, Thomas Imhof, Uwe Hansen, Nicodeme Paul, Raphael Carapito, Nicolas Pythoud, Aurelie Hirschler, Christine Carapito, Helene Dumortier, Christopher G. Mueller, Manuel Koch, Katja Schenke-Layland, Shigeyuki Kon, Anne Sudaka, Fabienne Anjuere, Ellen Van Obberghen-Schilling, Gertraud Orend
CANCER IMMUNOLOGY RESEARCH
(2020)
Article
Allergy
Adrien Brulefert, Astrid Hoste, Quentin Muller, Jean-Daniel Fauny, Christopher G. Mueller, Vincent Flacher
Summary: Our study found that vitamin D3 can induce skin dendritic cells to differentiate into Th2 cells producing IL-4 and IL-13, while also triggering the release of TSLP. Blocking the TSLP receptor in some donors eliminated IL-13 production but had no effect on IL-4+ Th2 cells. Vitamin D3-elicited CD14+ dendritic cells were able to promote IL-4+ Th2 cells in a TSLP-independent manner.
Letter
Dermatology
Adrien Brulefert, Melanie Kraemer, Marie Cumin, Amandine Selle, Astrid Hoste, Hans-Henrik Gad, Julia Ruhl, Jean-Baptiste Madinier, Olivier Chaloin, Christian Munz, Philippe Despres, ChristopherGeorge Mueller, Vincent Flacher
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2021)
Review
Immunology
Christopher G. Mueller, Christian Gaiddon, Aina Venkatasamy
Summary: In summary, immuno-therapy has the potential to become the standard neoadjuvant procedure for many cancers, but it is essential to consider the identity, organization, and activation status of immune cells. Although there have been advancements in imaging technologies for immune cells, further research and technological improvements are needed for its clinical implementation.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Abdouramane Camara, Alice C. Lavanant, Jun Abe, Henri Lee Desforges, Yannick O. Alexandre, Erika Girardi, Zinaida Igamberdieva, Kenichi Asano, Masato Tanaka, Thomas Hehlgans, Klaus Pfeffer, Sebastien Pfeffer, Scott N. Mueller, Jens Stein, Christopher G. Mueller
Summary: CD169+ macrophages in lymph nodes and spleen require dual signals from LT beta R and RANK for their differentiation. RANKL is necessary for the formation of their niche in lymph nodes, while splenic marginal zone stromal cells provide the source of RANKL for marginal metallophilic macrophage differentiation in the spleen. Loss of these macrophages compromises viral capture and the expansion of virus-specific CD8+ T cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Multidisciplinary Sciences
Charlotte Sornay, Valentine Vaur, Alain Wagner, Guilhem Chaubet
Summary: The bioconjugation of proteins, a complex process due to their sensitivity and selectivity issues, has been extensively studied. Strategies and reagents have been developed to address chemoselectivity and site-selectivity aspects. This review provides an overview of these strategies, with a focus on utilizing native proteins as substrates.
ROYAL SOCIETY OPEN SCIENCE
(2022)
Article
Biochemical Research Methods
Tony Rady, Lorenzo Turelli, Marc Nothisen, Elisabetta Tobaldi, Stephane Erb, Fabien Thoreau, Oscar Hernandez-Alba, Sarah Cianferani, Francois Daubeuf, Alain Wagner, Guilhem Chaubet
Summary: Cleavable linkers have emerged as a vital tool in chemical biology, particularly in the development of antibody-drug conjugates. Acid-labile motifs, including hydrazone and carbonate linkers, have received significant attention due to their ability to facilitate drug release in lysosomes. In this study, a novel family of cyclic acetals with excellent plasma stability and acid lability, especially in lysosomes, was designed. These linkers, when incorporated into ADC, showed potent in vitro anti-tumor activity and in vivo efficacy comparable to the commercially available ADC Kadcyla in mouse xenograft models.
BIOCONJUGATE CHEMISTRY
(2022)
Article
Chemistry, Analytical
Robin Dufossez, Sylvain Ursuegui, Stephanie Baudrey, Ketty Pernod, Safae Mouftakhir, Mustapha Oulad-Abdelghani, Michel Mosser, Guilhem Chaubet, Michael Ryckelynck, Alain Wagner
Summary: Enzyme-linked immunosorbent assay (ELISA) is a widely used method for protein detection in biological science. In this study, a novel droplet-based immunoassay format called D-SIRe was developed, which utilizes the oil/water interface as the capture and detection surface for proteins. This method is fast and sensitive, allowing the detection of analytes at the single bacteria level.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Janina Sponsel, Yubing Guo, Lutfir Hamzam, Alice C. Lavanant, Annia Perez-Riveron, Emma Partiot, Quentin Muller, Julien Rottura, Raphael Gaudin, Dirk Hauck, Alexander Titz, Vincent Flacher, Winfried Roemer, Christopher G. Mueller
Summary: Pseudomonas aeruginosa produces a virulence factor, LecB, that binds to endothelial cells and disrupts leukocyte passage, impairing immune response. The lecturer also impacts dendritic cell migration and T cell activation. Inhibiting LecB can restore immune response, highlighting its importance in combating P. aeruginosa infection.
Article
Immunology
Victoria N. Cousin, Guillermo F. Perez, Kathryn J. Payne, Reinhard E. Voll, Marta Rizzi, Christopher G. Mueller, Klaus Warnatz
Summary: Non-hematopoietic lymphoid stromal cells (LSC) play a crucial role in maintaining lymph node structure and facilitating immune cell migration, activation, and survival. These cells exhibit heterogeneous properties and secrete various factors that support different activities of the adaptive immune response. LSCs are involved in antigen transport, cell migration, and the formation of germinal centers, and are important for the development of humoral immunodeficiency and autoimmunity.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Nanoscience & Nanotechnology
Robin Dufossez, Marie-Pierre Krafft, Sylvain Ursuegui, Michel Mosser, Safae Mouftakhir, Ketty Pernod, Guilhem Chaubet, Michael Ryckelynck, Alain Wagner
Summary: Droplet-based microfluidics is advancing the development of miniaturized and sensitive enzyme-linked immunosorbent assays (ELISAs). This article investigates the impact of surfactant structure on the efficiency and stability of the antibody grafting process on droplets. It demonstrates that short linkers lead to high functionalization efficiency, while long linkers enhance stability, with an intermediate size required to balance both parameters. The optimal structure is a PEG4 linker connecting a polar di-azide head and a per-fluoropolyether tail.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Analytical
Robin Dufossez, Sylvain Ursuegui, Stephanie Baudrey, Ketty Pernod, Safae Mouftakhir, Mustapha Oulad-Abdelghani, Michel Mosser, Guilhem Chaubet, Michael Ryckelynck, Alain Wagner
Summary: Enzyme-linked immunosorbent assay (ELISA) is an important method for detecting proteins in biological science. By utilizing droplet-based microfluidics, miniaturized, less-consuming, and more sensitive ELISA assays can be developed by encapsulating biological samples and antibody-functionalized particles. This study introduces a new droplet surface immunoassay format that uses the oil/water phase interface as a protein capture and detection surface, eliminating the need for particles. The resulting method, called droplet surface immunoassay by fluorescence relocation (D-SIRe), demonstrates high sensitivity and rapid detection capabilities.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Tony Rady, Michel Mosser, Marc Nothisen, Stephane Erb, Igor Dovgan, Sarah Cianferani, Alain Wagner, Guilhem Chaubet
Summary: BCN alcohol is a significant strained-alkyne scaffold in chemical biology. This study presents the synthesis of an oxidized analogue - BCN acid - which can be easily functionalized via amide bond formation, resulting in more stable derivatives than traditionally encountered carbamates.
Article
Chemistry, Organic
David Warther, Enes Dursun, Marion Recher, Sylvain Ursuegui, Michel Mosser, Joanna Sobska, Wojciech Krezel, Guilhem Chaubet, Alain Wagner
Summary: SPAAC is an important member of bioorthogonal reactions, and conducting SPAAC in human plasma can greatly accelerate reaction rates and achieve selective couplings between specific reagents.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2021)