Editorial Material
Multidisciplinary Sciences
Jochen Balbach, Milton T. Stubbs
Summary: Phage protein E kills bacteria by shutting down cell wall biosynthesis.
Article
Virology
Elizabeth T. Ogunbunmi, Aaron P. Roznowski, Bentley A. Fane
Summary: Most icosahedral viruses condense their genomes into volume-constrained capsids, but ssDNA viruses have specific genome biosynthesis and packaging mechanisms. The packaging of ssDNA genomes combines elements from both dsDNA and ssRNA systems, potentially affecting both packaging and postpackaging phenomena.
JOURNAL OF VIROLOGY
(2021)
Article
Food Science & Technology
Yi Ma, Liu Cui, Meng Wang, Qiuli Sun, Kaisheng Liu, Jufang Wang
Summary: This study compared the lysis efficiency of different Protein E variants in E. coli, resulting in improved production of high-quality BGs. The use of pLysS plasmid led to nearly 100% lysis efficiency, yielding BGs with higher quality compared to conventional methods. This novel approach was successfully applied to produce high-quality bacterial ghosts for potential biomedical applications.
Article
Chemistry, Multidisciplinary
Sofia Margiola, Karola Gerecht, Manuel M. Mueller
Summary: This study presents a protein semi-synthesis approach to investigate site-specifically modified p53, providing a new method for a better understanding of how p53 is controlled by PTMs.
Article
Biochemistry & Molecular Biology
Lin Xue, Paul Schnacke, Michelle S. Frei, Birgit Koch, Julien Hiblot, Richard Wombacher, Sebastian Fabritz, Kai Johnsson
Summary: This study introduces the first biosensor for measuring CoA levels in different organelles of mammalian cells and explores the roles of various proteins involved in CoA biosynthesis and transport. The findings reveal the mechanism of maintaining pools of CoA in cells.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Chemistry, Analytical
Yan Shan Ang, Lin-Yue Lanry Yung
Summary: Protein-oligonucleotide conjugates are crucial in various biological applications. This study demonstrates the importance of high-quality conjugate probes for achieving consistent performance. A streamlined workflow was developed to obtain pure subpopulations of protein probes carrying a specific number of oligonucleotide strands. This workflow was successfully applied to a wide range of binders and showed robust assay performance with just a single reaction step and short reaction time.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Alexander T. Duckworth, Tricia A. Windgassen, James L. Keck
Summary: Bacteria use DNA replication restart mechanisms to reload replisomes onto abandoned replication forks when encountering barriers, to prevent incomplete DNA replication and lethality. The Escherichia coli PriA DNA helicase plays a crucial role in this process by recognizing and remodeling replication forks, with a conserved sequence motif potentially serving as an autoregulatory element. Multiple PriA molecules may work in tandem to enhance DNA unwinding processivity, showing unexpected similarities with other DNA helicases.
Article
Construction & Building Technology
Jung Hoon Lee, Hwan Kim, Hyoung-Il Kim, Kook Hyun Yu
Summary: Devices for the filtration and sterilization of indoor spaces have been widely used due to the COVID-19 outbreak. This study aimed to identify important factors for evaluating air purifier efficiency in large chambers. The distribution characteristics and natural reduction of airborne viruses were investigated, and it was found that the airborne-virus removal efficiency should be evaluated after 40 minutes for accuracy. Additionally, the sampling efficiencies of different samplers were compared, with the impactor showing the highest efficiency and lowest standard deviation.
INDOOR AND BUILT ENVIRONMENT
(2023)
Article
Biochemical Research Methods
Maruthi Prasanna, Ruben Varela Calvino, Annie Lambert, Maria Arista Romero, Sylvia Pujals, Francois Trottein, Emilie Camberlein, Cyrille Grandjean, Noemi Csaba
Summary: This study demonstrates the potential of chitosan-based nanovaccines for efficient delivery of glycoconjugate antigens. Encapsulated glycoconjugates showed enhanced uptake by cells and better immunostimulatory properties compared to naked antigens. The study also showed a 100-fold increase in IgG response with the encapsulated glycoconjugate compared to the nonencapsulated group in mice.
BIOCONJUGATE CHEMISTRY
(2023)
Article
Biochemical Research Methods
Maruthi Prasanna, Ruben Varela Calvino, Annie Lambert, Maria Arista Romero, Sylvia Pujals, Francois Trottein, Emilie Camberlein, Cyrille Grandjean, Noemi Csaba
Summary: This study demonstrates the potential of chitosan-based nanovaccine for efficient delivery of glycoconjugate antigens. The encapsulation of the glycoconjugate using chitosan nanoparticles (CNPs) enhanced its immunogenicity and resulted in a significantly higher immune response compared to the nonencapsulated glycoconjugate. These findings suggest that CNPs could be a promising delivery system for pneumococcal conjugate vaccines.
BIOCONJUGATE CHEMISTRY
(2023)
Article
Cell Biology
Kaining Zhang, Shanshan Li, Yawen Wang, Zhihao Wang, Nancy Mulvenna, Hang Yang, Peipei Zhang, Huan Chen, Yan Li, Hongliang Wang, Yongxiang Gao, Sivaramesh Wigneshweraraj, Steve Matthews, Kaiming Zhang, Bing Liu
Summary: In this study, we report a phage-encoded enolase inhibitor protein, Gp60, which can inhibit bacterial growth and peptidoglycan synthesis by disrupting the structure of enolase. This phage-inspired disassembly of enolase represents an alternative strategy for the development of antimicrobial drugs.
Article
Multidisciplinary Sciences
Alexander T. T. Duckworth, Peter L. L. Ducos, Sarah D. D. McMillan, Kenneth A. A. Satyshur, Katelien H. H. Blumenthal, Haley R. R. Deorio, Joseph A. A. Larson, Steven J. J. Sandler, Timothy Grant, James L. L. Keck
Summary: Bacteria have evolved replication restart pathways to reload replisomes onto prematurely terminated replication forks. In this study, the cryogenic-electron microscopy structure of a PriA/PriB/replication fork complex was determined, revealing the replication fork specificity and the switch-like mechanism for replication restart initiation. This research provides important insights into robust and high-fidelity replication re-initiation.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Multidisciplinary
Koji Hashimoto, Emil C. Fischer, Floyd E. Romesberg
Summary: The researchers have developed semisynthetic organisms that can store and retrieve increased information by using a series of synthetic, unnatural base pairs. Their results demonstrate that the E. coli RNA polymerase can transcribe DNA containing unnatural base pairs, and these advancements should facilitate the creation of next generation SSOs.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Cell Biology
Erica A. Birkholz, Thomas G. Laughlin, Emily Armbruster, Sergey Suslov, Jina Lee, Johannes Wittmann, Kevin D. Corbett, Elizabeth Villa, Joe Pogliano
Summary: Nucleus-forming jumbo phages establish an intricate subcellular organization, enclosing phage genomes within a proteinaceous shell called the phage nucleus. This work identifies a coherent assembly of filaments into a vortex-like structure driving intracellular rotation within the prokaryotic cytoplasm.
Article
Chemistry, Analytical
Xiaojia Jiang, Tiezheng Pan, Chao Lang, Chao Zeng, Jinxing Hou, Jiayun Xu, Quan Luo, Chunxi Hou, Junqiu Liu
Summary: In this study, a semisynthetic selenoenzyme nanoreactor was created to monitor and analyze intermediates in enzymatic reactions, revealing the mysteries of selenoenzyme catalysis at the single-molecule level.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Tsutomu Sugihara, Masayasu Mie, Eiry Kobatake
ANALYTICAL BIOCHEMISTRY
(2018)
Article
Biotechnology & Applied Microbiology
Yasumasa Mashimo, Masayasu Mie, Eiry Kobatake
BIOTECHNOLOGY LETTERS
(2018)
Article
Biotechnology & Applied Microbiology
Masayasu Mie, Takahiro Niimi, Yasumasa Mashimo, Eiry Kobatake
BIOTECHNOLOGY LETTERS
(2019)
Article
Biochemistry & Molecular Biology
Masayasu Mie, Rie Matsumoto, Yasumasa Mashimo, Anthony E. G. Cass, Eiry Kobatake
MOLECULAR BIOLOGY REPORTS
(2019)
Article
Biochemistry & Molecular Biology
Yoshinori Mizuguchi, Yasumasa Mashimo, Masayasu Mie, Eiry Kobatake
Article
Chemistry, Analytical
Tsutomu Sugihara, Masayasu Mie, Eiry Kobatake
ANALYTICAL SCIENCES
(2020)
Article
Engineering, Biomedical
Afroza Sharmin, Nihad Adnan, Amranul Haque, Yasumasa Mashimo, Masayasu Mie, Eiry Kobatake
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
(2020)
Article
Nanoscience & Nanotechnology
Wei Guo, Yasumasa Mashimo, Eiry Kobatake, Masayasu Mie
Article
Multidisciplinary Sciences
Bijon Kumar Sil, Nowshin Jahan, Md Ahsanul Haq, Mumtarin Jannat Oishee, Tamanna Ali, Shahad Saif Khandker, Eiry Kobatake, Masayasu Mie, Mohib Ullah Khondoker, Mohd Raeed Jamiruddin, Nihad Adnan
Summary: In this study, a rapid and specific SARS-CoV-2 ELISA was developed and evaluated for detecting anti-SARS-CoV-2 IgG in patient samples. The assay demonstrated high sensitivity and specificity, making it a promising tool for early and retrospective sero-monitoring in areas affected by the pandemic.
Article
Infectious Diseases
Nihad Adnan, Shahad Saif Khandker, Ahsanul Haq, Mousumi Akter Chaity, Abdul Khalek, Anawarul Quader Nazim, Taku Kaitsuka, Kazuhito Tomizawa, Masayasu Mie, Eiry Kobatake, Sohel Ahmed, Nor Azlina A. Ali, Mohib Ullah Khondoker, Mainul Haque, Mohd Raeed Jamiruddin
Summary: This study investigated the correlation of Ct value, storage temperature, and conditions with SARS-CoV-2 diagnosis using a newly developed ELISA system. Results showed that the ELISA system had highest sensitivity for samples with Ct <= 30 and stored at -80 degrees C, with specificity ranging between 98.3% and 100%. Early infection phase diagnosis and lower temperature preservation of samples are crucial for rapid antigen ELISA tests.
EXPERT REVIEW OF ANTI-INFECTIVE THERAPY
(2022)
Article
Immunology
Mohd Raeed Jamiruddin, Md Ahsanul Haq, Kazuhito Tomizawa, Eiry Kobatake, Masayasu Mie, Sohel Ahmed, Shahad Saif Khandker, Tamanna Ali, Nowshin Jahan, Mumtarin Jannat Oishee, Mohib Ullah Khondoker, Bijon Kumar Sil, Mainul Haque, Nihad Adnan
Summary: This study observed antibody dynamics in individuals exposed to SARS-COV-2, finding that COVID-19 patients experienced more severe symptoms during their second episode, accompanied by a significant increase in IgG antibodies. IgM and IgA antibodies in the blood may wane quickly, while IgG antibodies persist. The findings suggest potential implications for the development of sero-diagnostics and predictions for ongoing vaccination efforts.
JOURNAL OF INFLAMMATION RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Masayasu Mie, Rena Hirashima, Yasumasa Mashimo, Eiry Kobatake
APPLIED SCIENCES-BASEL
(2020)
Meeting Abstract
Biochemistry & Molecular Biology
Shin-Ichiro Yokota, Yasumasa Mashimo, Yoshio Hamada, Youji Harada, Masayasu Mie, Eiry Kobatake, Kenji Usui
JOURNAL OF PEPTIDE SCIENCE
(2018)