Journal
BIOCONJUGATE CHEMISTRY
Volume 21, Issue 12, Pages 2234-2238Publisher
AMER CHEMICAL SOC
DOI: 10.1021/bc100215j
Keywords
-
Categories
Funding
- Hellman Family Foundation
- California Nanosystems Institute
Ask authors/readers for more resources
An optical encoder is a device that uses an interrupted light source sensor pair to map linear or rotational motion onto a periodic signal. Simple, inexpensive optical encoders are used for precise positioning in machines such as desktop printers, disk drives, and astronomical telescopes. A strand of DNA labeled with a series of Forster resonance energy transfer acceptor dyes can perform the same function at the nanometer scale, producing a periodic fluorescence signal that encodes the movement of a single donor-labeled molecular motor with high spatial and temporal resolution. Previous measurements of this type have employed encoders limited to five acceptor dyes. and hence five signal periods, restricting the range of motion that could be followed. Here we describe two methods for synthesizing double-stranded DNA containing several to hundreds of regularly spaced dyes on one strand. Distinct functional groups incorporated at the encoder ends enable tethering for single-molecule measurements.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available