Article
Endocrinology & Metabolism
P. Maria Clemente-Olivo, J. Jayron Habibe, Mariska Vos, Roelof Ottenhoff, Aldo Jongejan, Hilde Herrema, Noam Zelcer, Sander Kooijman, Patrick C. N. Rensen, Daniel H. van Raalte, Max Nieuwdorp, Etto C. Eringa, J. Carlie de Vries
Summary: The study reveals that the FHL2 gene is associated with obesity and energy metabolism. FHL2 deficiency protects mice from high-fat diet-induced weight gain while glucose handling remains normal. Enhanced energy expenditure was observed in multiple tissues.
METABOLISM-CLINICAL AND EXPERIMENTAL
(2021)
Review
Pharmacology & Pharmacy
Sara Lovisa
Summary: EMT, a process crucial in wound healing, fibrosis, and cancer, has gained significant attention from researchers since its discovery in the seventies. Despite advances in understanding its functional role, targeting EMT in fibrotic diseases remains a challenge at an early stage of development.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Immunology
Sarthak Sahoo, Sonali Priyadarshini Nayak, Kishore Hari, Prithu Purkait, Susmita Mandal, Akash Kishore, Herbert Levine, Mohit Kumar Jolly
Summary: Recent research shows that cells with hybrid epithelial/mesenchymal characteristics can have high levels of PD-L1, important for immune evasion, without needing to fully undergo epithelial-mesenchymal transition. This cellular state in breast cancer is associated with resistance to anti-estrogen therapy.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Lili Liang, Andreas M. Kaufmann
Summary: Cancer stem cells (CSCs) have been identified and characterized in both hematopoietic and solid tumors. They possess unique properties, including slow proliferation, self-renewal, differentiation, and the ability to undergo epithelial-mesenchymal transition (EMT) and its reverse process. CSCs play a crucial role in tumor growth, therapy resistance, and cancer metastasis. Strategies targeting CSCs are being developed for novel anti-metastasis treatments, aiming to improve therapeutic efficiency and prognosis with fewer side effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Joanna Kozak, Alicja Forma, Marcin Czeczelewski, Pawel Kozyra, Elzbieta Sitarz, Elzbieta Radzikowska-Buchner, Monika Sitarz, Jacek Baj
Summary: Epithelial-mesenchymal transition (EMT) is a key process in the development of gastric cancer, affecting patient outcomes. There is an urgent need for research on pharmacological agents targeting EMT to prevent progression and potential metastasis of gastric cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Anastasios Goulioumis, Kostis Gyftopoulos
Summary: Epithelial-to-mesenchymal transition (EMT) is a molecular phenomenon that occurs in epithelial neoplasms, such as laryngeal carcinoma. EMT leads to the loss of epithelial traits and acquisition of mesenchymal traits by tumor cells, enhancing their migratory capacity. EMT is mediated by complex molecular pathways and involves various changes, including loss of adhesion, cytoskeleton remodeling, evasion of apoptosis and immune surveillance, upregulation of metalloproteinases, etc. Partial EMT models have been accepted to explain the cell plasticity associated with invasion and metastasis of tumors.
Review
Oncology
Tuan Luu
Summary: Pancreatic cancer, known for its high mortality and insensitivity to existing therapies, has attracted increasing scholarly attention for its progression mechanisms, particularly the role of epithelial-mesenchymal transition (EMT) in cancer genesis and metastasis. Recent discoveries on EMT-regulating factors in pancreatic cancer have shed light on the relationship between EMT and this cancer type, marking a key advancement in research on EMT in pancreatic cancer.
FRONTIERS IN ONCOLOGY
(2021)
Review
Pharmacology & Pharmacy
Zhi Xiong Chong, Swee Keong Yeap, Wan Yong Ho
Summary: Osteosarcoma, a prevalent primary bone tumor, has high metastatic potential and poor prognosis. MiRNAs play crucial roles in regulating the EMT process in osteosarcoma, which may offer insights for prognostic and therapeutic strategies.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Engineering, Biomedical
Adrian Cervero-Varona, Angelo Canciello, Alessia Peserico, Arlette Alina Haidar Montes, Maria Rita Citeroni, Annunziata Mauro, Valentina Russo, Samanta Moffa, Serena Pilato, Stefano Di Giacomo, Beatrice Dufrusine, Enrico Dainese, Antonella Fontana, Barbara Barboni
Summary: The use of biomaterials in immune regenerative strategies for unresolved pathologies is gaining attention in tissue engineering. Graphene oxide (GO) has been proposed as an immune-mimetic material for stem cell-based regenerative therapies, influencing stem cell behavior and immune response. Amniotic epithelial stem cells (AECs) are also of interest due to their plasticity and immunomodulatory paracrine activities, and the effects of GO on AECs are explored in this study. GO-coating induced and accelerated the Epithelial-Mesenchymal Transition (EMT) in AECs, leading to changes in cytokine secretion and enhanced activation of macrophages. Adhesion of AECs on GO-functionalized surfaces could create a tailored microenvironment for the regenerative process.
MATERIALS TODAY BIO
(2023)
Article
Biochemistry & Molecular Biology
Yuan Zhang, Aurore Devocelle, Christophe Desterke, Lucas Eduardo Botelho de Souza, Eva Hadadi, Herve Acloque, Adlen Foudi, Yao Xiang, Annabelle Ballesta, Yunhua Chang, Julien Giron-Michel
Summary: The study demonstrates that BMAL1 plays a crucial role in maintaining the balance between epithelial and mesenchymal properties of CRC cell lines, influencing their capacities for adhesion, migration, invasion, and chemoresistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Roslyn Tedja, Ayesha B. Alvero, Alexandra Fox, Carlos Cardenas, Mary Pitruzzello, Hussein Chehade, Tejeshwhar Bawa, Nicholas Adzibolosu, Radhika Gogoi, Gil Mor
Summary: This study established an in vitro model of epithelial-to-mesenchymal transition (EMT) and identified the main genomic signature associated with the E/M hybrid state. The results showed that cells in the E/M hybrid state gain stable anoikis resistance, invasive capacity, and tumorigenic potential. The hepatocyte growth factor (HGF)/c-MET pathway was identified as a major driver of cells in the E/M hybrid state.
Review
Oncology
Evan M. McCabe, Theodore P. Rasmussen
Summary: The epithelial to mesenchymal transition (EMT) is a cellular process crucial in embryogenesis, where cancer stem cells (CSCs) play a role by differentiating and undergoing EMT. Long noncoding RNAs (lncRNAs) also have a role in CSCs and EMT, with the potential to serve as biomarkers and therapeutic targets in cancer progression.
SEMINARS IN CANCER BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Rui Yu, Yan Wu, Ping He, Yu Bai, Yongzhe Zhang, Xiaohui Bian, Guangping Sun, Beiru Zhang
Summary: Renal fibrosis is the main pathology of chronic kidney disease and LMCD1 is upregulated in patients with CKD and treated kidney cells. Knockdown of LMCD1 can improve renal fibrosis by blocking the activation of the extracellular signal-regulated kinase pathway. The study further investigates the effect of LMCD1 on TGF-01-induced epithelial-mesenchymal transition (EMT) and its potential role in the TGF-01/Smad signaling pathway.
LABORATORY INVESTIGATION
(2023)
Article
Biochemical Research Methods
Ayalur Raghu Subbalakshmi, Sarthak Sahoo, Prakruthi Manjunatha, Shaurya Goyal, Vignesh A. Kasiviswanathan, Yeshwanth Mahesh, Soundharya Ramu, Isabelle McMullen, Jason A. A. Somarelli, Mohit Kumar Jolly
Summary: ELF3 is a factor strongly associated with an epithelial phenotype and is inhibited during EMT. It inhibits the progression of EMT and can counteract EMT induction even in the presence of EMT-inducing factors. The prognostic capacity of ELF3 is specific to cell-of-origin or lineage.
JOURNAL OF BIOLOGICAL ENGINEERING
(2023)
Review
Biochemistry & Molecular Biology
Azine Datlibagi, Anna Zein-El-Din, Maxime Frohly, Francois Willermain, Christine Delporte, Elie Motulsky
Summary: Proliferative vitreoretinal diseases (PVDs), such as proliferative vitreoretinopathy (PVR), epiretinal membranes, and proliferative diabetic retinopathy, are characterized by the formation of proliferative membranes due to EMT of retinal pigment epithelium (RPE) and endothelial cells. In vitro models, including cell lines, stem-cell-derived RPE, and primary cells, are utilized to induce EMT and mimic PVD. In vivo PVR animal models are obtained through surgical means or intravitreal injection to investigate EMT and cell behavior. This review provides a comprehensive overview of the current models available for studying EMT in PVD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Cell Biology
M. Leonor Cancela, Vincent Laize, Natercia Conceicao, Herve Kempf, Monzur Murshed
Summary: Keutel syndrome is a rare genetic disorder characterized by abnormal calcification of cartilaginous tissues and cardiovascular defects. Research has shown that pathological mineral deposition is the primary cause of these abnormalities, but the mechanisms by which MGP prevents abnormal calcification remain poorly understood.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Tatiana Varela, Natercia Conceicao, Vincent Laize, M. Leonor Cancela
Summary: This study analyzed the regulatory mechanisms of DUSP4 promoters 1 and 2 by cancer-related transcription factors, finding that the TFs have promoter-specific activity in regulating DUSP4 expression. These insights contribute to a better understanding of DUSP4 dysregulation in various types of cancer.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2021)
Article
Environmental Sciences
Katia Pes, Annika Friese, Cymon J. Cox, Vincent Laize, Ignacio Fernandez
Summary: The study found that exposure to three pharmaceuticals had effects on antioxidant status and gene expression in mussels, raising concerns about the release of these drugs into the aquatic environment. Further research is needed to accurately assess the environmental risks of these poorly studied drugs.
MARINE ENVIRONMENTAL RESEARCH
(2021)
Review
Cell Biology
Joana T. Rosa, Vincent Laize, Paulo J. Gavaia, M. Leonor Cancela
Summary: Osteopenia and osteoporosis are bone disorders characterized by reduced bone mineral density, altered bone microarchitecture, and increased bone fragility. The global aging population has led to a rapid increase in their incidence, driving the need for more effective treatments. Teleost fish, particularly zebrafish and medaka, have emerged as suitable alternatives for studying bone formation and mineralization, and for modeling human bone disorders, offering technical advantages that accelerate research and model human osteopenia and osteoporosis phenotypes efficiently.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Nutrition & Dietetics
Vania P. Roberto, Gwladys Surget, Klervi Le Lann, Sara Mira, Marco Tarasco, Fabienne Guerard, Nathalie Poupart, Vincent Laize, Valerie Stiger-Pouvreau, M. Leonor Cancela
Summary: The study found that the ethyl acetate fraction rich in polyphenols from Spartina alterniflora has significant antioxidant and bone anabolic activities, potentially serving as a novel source for therapeutic applications in bone disorders.
FRONTIERS IN NUTRITION
(2021)
Article
Environmental Sciences
Marco Tarasco, Paulo J. Gavaia, Anabela Bensimon-Brito, Joao Cardeira-da-Silva, Srinath Ramkumar, Fabrice P. Cordelieres, Stefan Guenther, Maria J. Bebianno, Didier Y. R. Stainier, M. Leonor Cancela, Vincent Laize
Summary: Persistent and ubiquitous organic pollutants, like BaP, pose a major threat to aquatic organisms and human health, with adverse effects on fish bone formation and skeletal development. BaP induces activation of xenobiotic and metabolic pathways, impacting extracellular matrix formation, and regulating inflammation markers in larvae. BaP exposure negatively affects bone remodeling and may involve a role for neutrophils in inflammatory bone reduction.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Environmental Sciences
Katia Pes, Juan B. Ortiz-Delgado, Carmen Sarasquete, Vincent Laize, Ignacio Fernandez
Summary: The marine habitat and biodiversity can be affected by released pharmaceuticals, as demonstrated by a study investigating the short-term effects of three commonly used drugs on juvenile Senegalese sole. The results showed drug-dependent effects on hemorrhages, histopathological alterations, antioxidant status, enzyme activities, and gene expression related to xenobiotic response. This study highlights the urgent need for an integrated risk assessment of the impact of pharmaceuticals on the aquatic environment.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2022)
Article
Environmental Sciences
Marco Tarasco, Paulo J. Gavaia, Anabela Bensimon-Brito, Fabrice P. Cordelieres, Tamara Santos, Gil Martins, Daniela T. de Castro, Nadia Silva, Elsa Cabrita, Maria J. Bebianno, Didier Y. R. Stainier, M. Leonor Cancela, Vincent Laize
Summary: The presence of microplastics in the aquatic ecosystem poses a major problem for the environment and human health. The capacity of organic pollutants to adsorb onto microplastic particles raises concerns, as it provides a new route for toxic compounds to enter the food web. This study provides new insights into the biological effects of pristine and/or contaminated microplastics on zebrafish.
Article
Food Science & Technology
Marcio Moreira, Lucia Solino, Catia L. Marques, Vincent Laize, Pedro Pousao-Ferreira, Pedro Reis Costa, Florbela Soares
Summary: A. ocellatum may produce toxin-like compounds that contribute to mortalities in fish. Extracts from the parasite's different life stages showed cytotoxic and hemolytic effects on cells.
Review
Chemistry, Medicinal
Joana T. Rosa, Marco Tarasco, Paulo J. Gavaia, M. Leonor Cancela, Vincent Laize
Summary: This review provides a comprehensive overview of zebrafish bone-related assays and their potential use in drug screening for mineralogenic/osteogenic compounds. The article also discusses tools for standardizing fish housing, embryo production synchronization, and automating specimen sorting and image acquisition/analysis for faster and more accurate screening outputs.
Article
Food Science & Technology
M. H. G. Berntssen, P. G. Fjeldal, P. J. Gavaia, V. Laize, K. Hamre, C. E. Donald, J. V. Jakobsen, A. Omdal, S. Soderstrom, K. K. Lie
Summary: The use of plant ingredients in novel aquafeeds has introduced mycotoxins to the farming of seafood. Two emerging mycotoxins, ENNB and BEA, have been found in these aquafeeds and farmed fish. The potential toxicity of ENNs and BEA in farmed fish and their transfer from feed to organs is not well understood. The study showed that both dietary BEA and ENNB had a low transfer rate in Atlantic salmon, with ENNB having a higher transfer rate. BEA caused growth reduction and decreased protein digestion and feed conversion rate, while ENNB caused stunted growth unrelated to feed utilization capacity. Lower levels of ENNB in commercial salmon feed could pose a risk to animal health, but not in the case of BEA.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Multidisciplinary Sciences
Joao Cardeira-da-Silva, Anabela Bensimon-Brito, Marco Tarasco, Ana S. Brandao, Joana T. Rosa, Jorge Borbinha, Paulo J. Almeida, Antonio Jacinto, M. Leonor Cancela, Paulo J. Gavaia, Didier Y. R. Stainier, Vincent Laize
Summary: By studying the bone patterning during zebrafish fin ray regeneration, it was discovered that the fin rays form through two mineralization fronts and bifurcation is not a simple splitting process. Osteolytic tubules play a key role in defining the position of the branchpoint.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Agriculture, Dairy & Animal Science
Elisa Padoan, Serena Ferraresso, Sara Pegolo, Carlo Barnini, Massimo Castagnaro, Luca Bargelloni
Summary: This study comprehensively defined the transcriptomic fingerprints of mild and severe equine asthma using gene expression analysis. The pathogenesis of equine asthma involves reduced mucociliary clearance and excessive complement activation leading to tissue injury. Furthermore, severe equine asthma is characterized by chronic inflammation and tissue remodeling similar to human asthma.
Review
Biochemistry & Molecular Biology
Joao M. A. Santos, Vincent Laize, Paulo J. Gavaia, Natercia Conceicao, M. Leonor Cancela
Summary: Ectopic calcification refers to the accumulation of calcium ions in soft tissues due to proteins dysfunction. The traditional model organism for studying abnormal calcium deposition, mice, often have limited understanding of the disease. The zebrafish model has gained popularity recently as it shares similarities with human pathological mineralization disorders and offers insights into potential therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Alessio Carletti, Joana T. Rosa, Katia Pes, Ines Borges, Tamara Santos, Luisa Barreira, Joao Varela, Hugo Pereira, M. Leonor Cancela, Paulo J. Gavaia, Vincent Laize
Summary: This study evaluated the impact of skeletal deformities on the aquaculture industry and found that certain strains of microalgae contain osteogenic and mineralogenic compounds. Ethanol extracts from these microalgae strains were found to promote fish bone development and improve skeletal health by reducing the incidence of skeletal anomalies.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Siva Santhiya Arul, Brinda Balakrishnan, Savithri S. Handanahal, Sangita Venkataraman
Summary: Viral nanoparticles are self-assembling delivery systems used for vaccines and therapeutic agents. They have improved biocompatibility and are widely used in various biomedical applications. However, large-scale production of VNPs still faces challenges.
Article
Biochemistry & Molecular Biology
Wisely Chua, Carl O. Marsh, Si En Poh, Winston LC. Koh, Melody Li Ying Lee, Li Fang Koh, Xin-Zi Emily Tang, Peter See, Zheng Ser, Shi Mei Wang, Radoslaw M. Sobota, Thomas L. Dawson Jr, Yik Weng Yew, Steven Thng, Anthony J. O'Donoghue, Hazel H. Oon, John E. Common, Hao Li
Summary: In this study, the gene expression of enzymes secreted by Malassezia globosa in the skin was investigated using targeted RNA sequencing. The study found that the expression of these enzymes is regulated by the fungus's environment and differs significantly from healthy skin sites. Additionally, a pseudoprotease MGL_3331 was identified to elicit an immune reaction in patients with atopic dermatitis. This highlights the importance of studying fungal proteins in physiologically relevant environments and their role in host immunity.
Article
Biochemistry & Molecular Biology
Belisa Parmeggiani, Marian Flores Signori, Cristiane Cecatto, Marina Rocha Frusciante, Manuela Bianchin Marcuzzo, Debora Guerini Souza, Rafael Teixeira Ribeiro, Bianca Seminotti, Diogo Onofre Gomes de Souza, Cesar Augusto Joa o Ribeiro, Moacir Wajner, Guilhian Leipnitz
Summary: Non ketotic hyperglycinemia (NKH) is a disorder caused by mutations in the genes encoding glycine cleavage system proteins, leading to severe neurodegeneration and white matter alterations. The study found that glycine affects myelin structure, glutamatergic system, and induces oxidative stress in neonatal rats and MO3.13 oligodendroglial cells.
Review
Biochemistry & Molecular Biology
Marie Rose Ndella Thiaw, Pascal Gantet
Summary: Mini zinc fingers play important roles in plant development, including control of cell division and expansion, meristem state transition, and growth and organ development. They have diverse modes of action, such as direct gene activation, interaction with specific transcription factors, and aggregation of protein complexes for chromatin remodeling. Further research is needed to explore their wider range of biological functions and their involvement in biotic and abiotic stress responses.
Article
Biochemistry & Molecular Biology
Shao-Jui Lai, Takahiro Kameda, Maasa Morita, Yuka Yamagata, Kaoruko Nishizaka, Yuna Horiuchi, Yukihiro Kobayashi, Yoko Usami, Jun-Jen Liu, Takeshi Kasama, Minoru Tozuka, Ryunosuke Ohkawa
Summary: In this study, the co-modification and impact of chymase and MPO on HDL were investigated. Sequential treatment with MPO and chymase generated two novel apoA-I fragments from HDL, one of which has a specific immunological property and can be used to predict patients with normal HDL-C levels and cardiovascular diseases.
Article
Biochemistry & Molecular Biology
Xiao-Mi Sun, Asami Yoshida, Takuya Ishii, Yan-Rong Jiang, Yi-Li Gao, Mikinori Ueno, Katsuya Hirasaka, Kiyoshi Osatomi
Summary: This study found that infection with the causative bacteria of edwardsiellosis, Edwardsiella tarda NUF251, leads to the production of superoxide radicals, causing oxidative stress. The research on the Jfsod1 gene suggests that the transcription factor NF-IL6 plays a significant role in upregulating the transcriptional activity of the gene in response to oxidative stress induced by NUF251 infection.
Article
Biochemistry & Molecular Biology
Shane M. Carey, Sean P. Kearns, Matthew E. Millington, Gregory S. Buechner, Beda E. Alvarez Jr, Leily Daneshian, Brendan Abiskaroon, Maksymilian Chruszcz, Edward L. D'Antonio
Summary: The importance of F337 in TcGlcK was investigated using the hole mutagenesis approach, revealing its key interactions with aromatic tail groups in inhibitor molecules. Mutants retaining activity and structural similarity were obtained, suggesting the possibility of TcHxK existing as a monomer lacking the F337 residue. Similar D-glucosamine inhibitors may bind less strongly to TcHxK due to the absence of van der Waals contacts from residue side chains.
Article
Biochemistry & Molecular Biology
Catia Barria, Diogo Athayde, Guillem Hernandez, Leonor Fonseca, Jorge Casinhas, Tiago N. Cordeiro, Margarida Archer, Cecilia M. Arraiano, Jose A. Brito, Rute G. Matos
Summary: Ribonucleases play a crucial role in RNA regulation by processing, degrading, and quality controlling cellular transcripts. Campylobacter jejuni, the main cause of human gastroenteritis, relies on the exoribonuclease PNPase (CjPNP) for low-temperature survival, virulence factor synthesis, and swimming, cell adhesion/invasion, and chick colonization abilities. The crystallographic structure of CjPNP, along with SAXS analysis, confirms its trimeric arrangement and provides insight into domain arrangement and flexibility. Mutations in highly conserved residues reveal unexpected RNA degradation activity even under conditions favoring polymerization. These findings have significant implications for the development of strategies to combat C. jejuni infections.
Article
Biochemistry & Molecular Biology
Elvira R. Shaykhutdinova, Maria S. Severyukhina, Inna V. Kholoshenko, Elena A. Gondarenko, Irina V. Shelukhina, Elena V. Kryukova, Alina M. Ismailova, Elena S. Sadovnikova, Igor A. Dyachenko, Arkady N. Murashev, Victor I. Tsetlin, Yuri N. Utkin
Summary: Evidence suggests that activating nicotinic acetylcholine receptors (nAChRs) can reduce cardiac injury caused by ischemia and reperfusion. In this study, the expression of a4, a6, and a132 nAChR subunits in the rat heart's left ventricle was demonstrated. The nAChR agonists cytisine and varenicline, commonly used for nicotine addiction treatment, were found to significantly reduce myocardial ischemia-reperfusion injury, with varenicline showing greater protection. The presence of a4132, a7, and/or a6132-containing receptors suggests that the protective effect of varenicline may be mediated by these subunits. These findings indicate a potential new use for cytisine and varenicline as cardioprotective agents.
Article
Biochemistry & Molecular Biology
Xinhai Xiong, Wenjie Chen, Cheng Chen, Qi Wu, Chaopeng He
Summary: This article summarizes the important roles of Cx43 in disease development from the perspective of subcellular localization and provides new ideas for Cx43 as a therapeutic target and the search for related pathological mechanisms.
Article
Biochemistry & Molecular Biology
Veronica Bastos, Simone Pascoal, Kamila Lopes, Marcia Mortari, Helena Oliveira
Summary: Cancer, especially melanoma, is a major global health problem due to its metastatic capacity, treatment resistance, and high mortality rates. The Chartergellus-CP1 peptide isolated from Chartergellus communis wasp venom showed selective cytotoxicity towards melanoma cell lines, inducing apoptosis, cell cycle impairment, and intracellular ROS increase.
Article
Biochemistry & Molecular Biology
Charlotte Claeyssen, Nathan Bulangalire, Bruno Bastide, Onnik Agbulut, Caroline Cieniewski-Bernard
Summary: Maintenance of striated muscle tissue relies on a dynamic network of protein-protein interactions. Desmin intermediate filaments play a crucial role in heart and skeletal muscle by maintaining cell structure and contributing to various cellular processes. The assembly of desmin intermediate filaments requires aB-crystallin, which is involved in multiple cellular functions.
Article
Biochemistry & Molecular Biology
Dhritiman Dey, Adhiraj Dasgupta, Dipanjan Ghosh, Oindrila Bhattacharjee, Abhrajyoti Ghosh, Ayae Honda, Dhrubajyoti Chattopadhyay
Summary: This study successfully identified host factors that interact with Chandipura Virus (CHPV) and play a critical role in infection, including A2M, LRP1, and GRP78. Knocking out A2M has a severe effect on viral infection, indicating the potential role of these host proteins in viral entry into host cells.
Article
Biochemistry & Molecular Biology
Anna Dotsenko, Igor Sinelnikov, Aleksandra Rozhkova, Ivan Zorov, Arkady Sinitsyn
Summary: Xylanases play important roles in various industrial applications, such as feed additives, pulp and paper bleaching, and the production of bread, food, and drinks. Xylanases from the GH10 family show high thermostability and resistance to protein inhibitors, making them promising candidates for these processes. This study successfully improved the thermostability and enzymatic activity of GH10 xylanase, which could enhance its effectiveness as a feed additive and during pulp and paper bleaching.
Article
Biochemistry & Molecular Biology
M. Jaquet, M. Bengue, K. Lambert, G. Carnac, D. Misse, C. Bisbal
Summary: Changes to our environment have led to the emergence of human pathogens such as chikungunya virus, which is now a major public health concern. In this study, the interconnection between skeletal muscle cells metabolism, their differentiation stage, and the infectivity of chikungunya virus was investigated. The results showed that chikungunya virus infectivity is dependent on cell differentiation and metabolism and that the virus interferes with cellular metabolism.
