Article
Chemistry, Medicinal
Javier R. Balboa, Dominik J. Essig, Sana Ma, Nichlas Karer, Louise S. Clemmensen, Soren W. Pedersen, Andreas C. Joerger, Stefan Knapp, Soren Ostergaard, Kristian Stromgaard
Summary: The complex of NMDAR, nNOS, and PSD-95 is an important therapeutic target for acute ischemic stroke. By designing a cyclic nNOS β-hairpin mimetic peptide and generating peptide arrays, a potent inhibitor of the nNOS/PSD-95 interaction was identified.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Boglarka Zambo, Gergo Gogl, Bastien Morlet, Pascal Eberling, Luc Negroni, Herve Moine, Gilles Trave
Summary: This study comprehensively investigates the PDZ-PBM interactome of diacylglycerol kinases (DGKs) using interactomic approaches and evolutionary analysis, revealing functional differences between PBMs of different types of DGKs.
Article
Multidisciplinary Sciences
Sachiko Fujiwara, Thanh Phuong Nguyen, Kyoko Furuse, Yugo Fukazawa, Tetsuhisa Otani, Mikio Furuse
Summary: The interaction of claudins with ZO proteins is not a prerequisite for tight junction formation at the most apical part of cell-cell contacts.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Mariano Martin, Lisa Salleron, Victoria Peyret, Romina Celeste Geysels, Elisabeth Darrouzet, Sabine Lindenthal, Carlos Eduardo Bernal Barquero, Ana Maria Masini-Repiso, Thierry Pourcher, Juan Pablo Nicola
Summary: NIS gene mutations leading to impaired iodide accumulation cause congenital hypothyroidism, as demonstrated by a truncated NIS protein missing some amino acids mislocalized into intracellular vesicles. Further investigation reveals the involvement of the PDZ domain in the interaction between the protein SCRIB and NIS, affecting the plasma membrane localization of NIS.
Article
Cell Biology
Michelle America, Naguissa Bostaille, Marie Eubelen, Maud Martin, Didier Y. R. Stainier, Benoit Vanhollebeke
Summary: This study reveals the molecular mechanisms underlying the interactions between Gpr124 and Frizzled in zebrafish and mammals, and provides insights into the evolution of Gpr124/Reck function in vertebrates.
Article
Microbiology
Yanlei Zhu, Flavio Alvarez, Nicolas Wolff, Ariel Mechaly, Sebastien Brule, Benoit Neitthoffer, Sandrine Etienne-Manneville, Ahmed Haouz, Batiste Boeda, Celia Caillet-Saguy
Summary: The C-terminus of the SARS-CoV-2 protein E contains a PDZ-binding motif (PBM) that interacts with human PDZ-containing proteins involved in cellular junctions and polarity. Crystal structures of complexes between human LNX2, MLLT4, and MPP5 PDZs and SARS-CoV-2 E PBM were solved, revealing the binding preferences of the PBM for cellular targets. Mutations in the E protein near the PBM may have important effects on its structure and ion-channel activity, as well as the host machinery targeted by the variants during infection.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jordi Gomez Borrego, Marc Torrent Burgas
Summary: Adhesion and colonization of host cells by pathogenic bacteria rely on protein-protein interactions (PPIs). This study developed a filtering process to obtain a reliable list of domains and motifs participating in PPIs between bacteria and human cells. Structural analysis revealed that human proteins involved in these interactions are rich in alpha helix and disordered regions. The most significant domain-domain interactions were validated, but verifying domain-motif interactions was more challenging. Post-translational modifications, especially phosphorylation, also play a role in these interactions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Mariano Genera, Baptiste Colcombet-Cazenave, Anastasia Croitoru, Bertrand Raynal, Ariel Mechaly, Joel Caillet, Ahmed Haouz, Nicolas Wolff, Celia Caillet-Saguy
Summary: The protein tyrosine phosphatase non-receptor type 3 (PTPN3) has a PDZ domain that plays both tumor-suppressive and tumor-promoting roles in cancer. This study focuses on the interactions between the PDZ domain of PTPN3 and the PDZ-binding motifs (PBMs) of viral and cellular protein partners. The study identifies key structural determinants of PBM recognition by PTPN3 and reveals the inhibitory role of the PDZ domain on the protein's phosphatase activity.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Multidisciplinary Sciences
Rebecca B. Berlow, H. Jane Dyson, Peter E. Wright
Summary: Intrinsically disordered proteins compete for binding to common regulatory targets to carry out their biological functions. The activation domains of HIF-1 alpha and CITED2 function as a unidirectional, allosteric molecular switch to control transcription of adaptive genes. The mechanistic details of this molecular switch were characterized through NMR spectroscopy and biophysical methods, revealing the contributions of individual binding motifs in CITED2. These findings provide insight into the complexity of molecular interactions involving disordered proteins and how they compete for occupancy of common targets.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Emilie Giraud, Chloe Otero del Val, Celia Caillet-Saguy, Nada Zehrouni, Cecile Khou, Joel Caillet, Yves Jacob, Nathalie Pardigon, Nicolas Wolff
Summary: The study revealed that the PBM of NS5 from West Nile virus interacts with PDZ-containing proteins in human cells, playing a crucial role in viral replication. Knockdown of specific PDZ-containing proteins resulted in decreased WNV replication, highlighting the significance of these interactions in the virus life cycle.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Lili Nian, Xuelu Liu, Yingbo Yang, Xiaolin Zhu, Xianfeng Yi, Fasih Ullah Haider
Summary: The LIM gene family in alfalfa plays a vital role in regulating various biological processes. Through comprehensive analysis, it was found that the 21 identified MsLIM genes can be classified into four subfamilies and share similar gene structures and conserved Motifs. Additionally, the distribution of these genes on chromosomes seems to be uneven and is influenced by segmental duplications.
Article
Biochemistry & Molecular Biology
Denis V. Petrovskiy, Kirill S. Nikolsky, Vladimir R. Rudnev, Liudmila I. Kulikova, Tatiana V. Butkova, Kristina A. Malsagova, Arthur T. Kopylov, Anna L. Kaysheva
Summary: This article introduces a new tool called SAFoldNet, which uses neural networks to search and align protein structures. The study shows that this method is competitive in searching and aligning protein structures, and a user-friendly web interface service has been developed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Shuo Wang, Jing Dong, Xue-Lei Zhao, Xin Song, Yue-Hong Long, Zhao-Bin Xing
Summary: This study identified 20 MBD genes in Eleutherococcus senticosus and found that they were unevenly distributed on different chromosomes, with fragment duplication being the main mechanism for gene expansion. The analysis showed that EsMBD genes were regulated mainly by light and anaerobic conditions. The results indicated that EsMBD2 and EsMBD5 jointly affected the secondary metabolic processes of E. senticosus saponins by binding to methylated CpG under drought stress conditions.
Article
Biochemistry & Molecular Biology
Guennadi Kozlov, Sandy Mattijssen, Jianning Jiang, Samuel Nyandwi, Tara Sprules, James R. Iben, Steven L. Coon, Sergei Gaidamakov, Anne M. Noronha, Christopher J. Wilds, Richard J. Maraia, Kalle Gehring
Summary: In this study, the La-module of LARP1 was characterized, and it was found that unlike other LARPs, the La-module of LARP1 does not contain an RRM domain. The La-module alone is capable of binding poly(A) RNA with high specificity for the RNA 3'-end. This study provides insights into the functional relevance of LARP1 RNA binding in cells.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Multidisciplinary Sciences
Boglarka Zambo, Bastien Morlet, Luc Negroni, Gilles Trave, Gergo Gogl
Summary: This article introduces a method called native holdup (nHU), which can directly estimate the equilibrium binding constants of protein interactions from cell extracts. Compared to other methods, nHU requires less sample preparation and can be combined with various analytical methods, making it widely applicable.
Article
Biology
Francois Kroll, Gareth T. Powell, Marcus Ghosh, Gaia Gestri, Paride Antinucci, Timothy J. Hearn, Hande Tunbak, Sumi Lim, Harvey W. Dennis, Joseph M. Fernandez, David Whitmore, Elena Dreosti, Stephen W. Wilson, Ellen J. Hoffman, Jason Rihel
Summary: A new method has been developed to rapidly knockout genes and shorten the experimental time from gene to behavioral phenotype in zebrafish from months to one week.
Article
Biochemistry & Molecular Biology
Shona Pfeiffer, Anna Tomascova, Uta Mamrak, Stefan J. Haunsberger, Niamh M. C. Connolly, Alexa Resler, Heiko Dussmann, Petronela Weisova, Elisabeth Jirstrom, Beatrice D'Orsi, Gang Chen, Mattia Cremona, Bryan T. Hennessy, Nikolaus Plesnila, Jochen H. M. Prehn
Summary: Progressive neuronal injury following ischaemic stroke is associated with AMPK activation, which leads to abnormal cellular responses to energetic stress. miR-210-3p plays a crucial role in regulating p70S6K activity in ischaemic injury and excitotoxic injury.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Dermatology
M. Scatolini, A. Patel, E. Grosso, M. Mello-Grand, P. Ostano, R. Coppo, M. Vitiello, T. Venesio, A. Zaccagna, A. Pisacane, I Sarotto, D. Taverna, L. Poliseno, D. Bergamaschi, G. Chiorino
Summary: The study identified a significant association between metastases, BRAF mutation, and low GJB5 expression in melanoma, suggesting a potential prognostic role for GJB5 in cutaneous melanoma.
BRITISH JOURNAL OF DERMATOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ivana Barravecchia, Chiara De Cesari, Mattia Forcato, Francesca Scebba, Olga Pyankova, Joanna M. Bridger, Helen A. Foster, Giovanni Signore, Andrea Borghini, Mariagrazia Andreassi, Massimiliano Andreazzoli, Silvio Bicciato, Mario Enrico Pe, Debora Angeloni
Summary: Microgravity and space radiation have distinct effects on human endothelial cells in space flight, triggering different molecular pathways and leading to specific changes in cell structure and function. Tailored countermeasures should be developed separately for microgravity and space radiation to protect astronauts' health effectively.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biology
Richard J. White, Eirinn Mackay, Stephen W. Wilson, Elisabeth M. Busch-Nentwich
Summary: In zebrafish, differentially expressed genes are often over-represented on the same chromosome as the mutation, due to different levels of expression of alleles from different genetic backgrounds. This has implications for interpreting the results and pathways in RNA-seq experiments.
Article
Anatomy & Morphology
Katherine J. Turner, Thomas A. Hawkins, Pedro M. Henriques, Leonardo E. Valdivia, Isaac H. Bianco, Stephen W. Wilson, Monica Folgueira
Summary: This study tracks and describes the development of different cell populations in the zebrafish telencephalon during the process of eversion using transgenic fish and structural markers, forming an atlas of telencephalic development. Additionally, they compare the transgenic expression with existing anatomical data.
FRONTIERS IN NEUROANATOMY
(2022)
Article
Cell & Tissue Engineering
Giulia Lottini, Matteo Baggiani, Giulia Chesi, Beatrice D'Orsi, Paola Quaranta, Michele Lai, Laura Pancrazi, Marco Onorati, Mauro Pistello, Giulia Freer, Mario Costa
Summary: This study found that Zika virus infection can cause changes in the localization and expression of transcription factor FOXG1, which is involved in cell replication and apoptosis. Growth factors and specific residues in FOXG1 played important roles in these processes. By deleting specific sequences, critical domains were identified. These results reveal the impact of Zika virus on FOXG1, its target genes, cell cycle progression, and survival of neural progenitor cells, contributing to microcephaly.
Article
Cell Biology
Franziska Walter, Beatrice D'Orsi, Anagha Jagannathan, Heiko Dussmann, Jochen H. M. Prehn
Summary: The Bcl-2 family protein BOK plays a critical role in regulating ER proteostasis and the cellular response to ER stress, as demonstrated by experiments showing an attenuated response in BOK-deficient cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell Biology
James Kealey, Heiko Dussmann, Irene Llorente-Folch, Natalia Niewidok, Manuela Salvucci, Jochen H. M. Prehn, Beatrice D'Orsi
Summary: The study reveals that activating KRAS mutations and loss of TP53 remodel cancer metabolism and lead to alterations in bioenergetics under metabolic stress conditions by modulating cellular ATP production, NADH oxidation, mitochondrial respiration and function.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Developmental Biology
Maria Hernandez-Bejarano, Gaia Gestri, Clinton Monfries, Lisa Tucker, Elena I. Dragomir, Isaac H. Bianco, Paols Bovlenia, Stephen W. Wilson, Florencia Cavodeassi
Summary: This study demonstrates the importance of appropriate retinal patterning during embryonic development for specific visual functions. The interplay of transcription factor Rx3 with Fibroblast Growth Factor and Hedgehog signals regulates the expression of specific regions, leading to the establishment of specialized visual features.
Article
Biochemistry & Molecular Biology
Chiara Gabellini, Cecilia Pucci, Chiara De Cesari, Davide Martini, Caterina Di Lauro, Matteo Digregorio, William Norton, Alessio Zippo, Alessandro Sessa, Vania Broccoli, Massimiliano Andreazzoli
Summary: The haploinsufficiency of the SETD5 gene, a histone methyltransferase, has been identified as a cause of intellectual disability and Autism Spectrum Disorder (ASD). Using zebrafish as a model, researchers found that zebrafish setd5 mutants displayed defective social behavior and indifference to social stimuli, which was rescued by the antipsychotic drug risperidone. These findings suggest that zebrafish setd5 mutants are a promising model for drug screening to reverse behavioral phenotypes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Oncology
Maria Laura Centomo, Marianna Vitiello, Laura Poliseno, Pier Paolo Pandolfi
Summary: MicroRNAs play a significant role in the genesis of cancer through their regulation of gene expression. MiR-22 can act as both an oncogene and a tumor suppressor gene, with its activity influenced by tissue and environmental factors. Previous studies have reported contradictory findings regarding the role of miR-22 in different cell and tissue types, but in immunocompetent model systems, miR-22 consistently acts as a tumor-promoting miRNA.
Article
Anatomy & Morphology
Davide Martini, Matteo Digregorio, Ilaria Anna Pia Voto, Giuseppe Morabito, Andrea Degl'Innocenti, Guido Giudetti, Martina Giannaccini, Massimiliano Andreazzoli
Summary: KDM7A is an important histone lysine demethylase that plays a critical role in embryonic development and pathological conditions. This study found that KDM7A is dynamically expressed throughout embryonic development and its overexpression affects the distribution of neuronal subtypes in retinal development.
DEVELOPMENTAL DYNAMICS
(2023)
Article
Multidisciplinary Sciences
Ivana Barravecchia, Chiara De Cesari, Viviana Guadagni, Giovanni Signore, Edoardo Bertolini, Serena Gea Giannelli, Francesca Scebba, Davide Martini, Mario Enrico Pe, Vania Broccoli, Massimiliano Andreazzoli, Debora Angeloni, Gian Carlo Demontis
Summary: In proliferating multipotent retinal progenitors, transcription factors play a role in determining the fate of postmitotic daughter cells. However, the influence of extrinsic factors on postmitotic cell fate plasticity is still unclear. Transcriptome analysis revealed the concurrent expression of genes critical for Muller glia cell fate in postmitotic rod precursors. Furthermore, the density of cell culture was found to regulate the expression of these genes, suggesting that cell culture density is an extrinsic factor that affects cell fate determination.
SCIENTIFIC REPORTS
(2023)
Article
Developmental Biology
Rebecca Wycliffe, Julie Plaisancie, Sydney Leaman, Octavia Santis, Lisa Tucker, Daniela Cavieres, Michelle Fernandez, Camila Weiss-Garrido, Cristian Sobarzo, Gaia Gestri, Leonardo E. Valdivia
Summary: The mab21l2(u517) mutant exhibits complex eye morphogenesis phenotype, characterized by delayed growth, excess progenitors, and asynchronous neurogenesis, suggesting a role of mab21l2 in coordinating optic cup development, timely neurogenesis, and progenitor allocation in zebrafish ciliary marginal zone.
INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Siva Santhiya Arul, Brinda Balakrishnan, Savithri S. Handanahal, Sangita Venkataraman
Summary: Viral nanoparticles are self-assembling delivery systems used for vaccines and therapeutic agents. They have improved biocompatibility and are widely used in various biomedical applications. However, large-scale production of VNPs still faces challenges.
Article
Biochemistry & Molecular Biology
Wisely Chua, Carl O. Marsh, Si En Poh, Winston LC. Koh, Melody Li Ying Lee, Li Fang Koh, Xin-Zi Emily Tang, Peter See, Zheng Ser, Shi Mei Wang, Radoslaw M. Sobota, Thomas L. Dawson Jr, Yik Weng Yew, Steven Thng, Anthony J. O'Donoghue, Hazel H. Oon, John E. Common, Hao Li
Summary: In this study, the gene expression of enzymes secreted by Malassezia globosa in the skin was investigated using targeted RNA sequencing. The study found that the expression of these enzymes is regulated by the fungus's environment and differs significantly from healthy skin sites. Additionally, a pseudoprotease MGL_3331 was identified to elicit an immune reaction in patients with atopic dermatitis. This highlights the importance of studying fungal proteins in physiologically relevant environments and their role in host immunity.
Article
Biochemistry & Molecular Biology
Belisa Parmeggiani, Marian Flores Signori, Cristiane Cecatto, Marina Rocha Frusciante, Manuela Bianchin Marcuzzo, Debora Guerini Souza, Rafael Teixeira Ribeiro, Bianca Seminotti, Diogo Onofre Gomes de Souza, Cesar Augusto Joa o Ribeiro, Moacir Wajner, Guilhian Leipnitz
Summary: Non ketotic hyperglycinemia (NKH) is a disorder caused by mutations in the genes encoding glycine cleavage system proteins, leading to severe neurodegeneration and white matter alterations. The study found that glycine affects myelin structure, glutamatergic system, and induces oxidative stress in neonatal rats and MO3.13 oligodendroglial cells.
Review
Biochemistry & Molecular Biology
Marie Rose Ndella Thiaw, Pascal Gantet
Summary: Mini zinc fingers play important roles in plant development, including control of cell division and expansion, meristem state transition, and growth and organ development. They have diverse modes of action, such as direct gene activation, interaction with specific transcription factors, and aggregation of protein complexes for chromatin remodeling. Further research is needed to explore their wider range of biological functions and their involvement in biotic and abiotic stress responses.
Article
Biochemistry & Molecular Biology
Shao-Jui Lai, Takahiro Kameda, Maasa Morita, Yuka Yamagata, Kaoruko Nishizaka, Yuna Horiuchi, Yukihiro Kobayashi, Yoko Usami, Jun-Jen Liu, Takeshi Kasama, Minoru Tozuka, Ryunosuke Ohkawa
Summary: In this study, the co-modification and impact of chymase and MPO on HDL were investigated. Sequential treatment with MPO and chymase generated two novel apoA-I fragments from HDL, one of which has a specific immunological property and can be used to predict patients with normal HDL-C levels and cardiovascular diseases.
Article
Biochemistry & Molecular Biology
Xiao-Mi Sun, Asami Yoshida, Takuya Ishii, Yan-Rong Jiang, Yi-Li Gao, Mikinori Ueno, Katsuya Hirasaka, Kiyoshi Osatomi
Summary: This study found that infection with the causative bacteria of edwardsiellosis, Edwardsiella tarda NUF251, leads to the production of superoxide radicals, causing oxidative stress. The research on the Jfsod1 gene suggests that the transcription factor NF-IL6 plays a significant role in upregulating the transcriptional activity of the gene in response to oxidative stress induced by NUF251 infection.
Article
Biochemistry & Molecular Biology
Shane M. Carey, Sean P. Kearns, Matthew E. Millington, Gregory S. Buechner, Beda E. Alvarez Jr, Leily Daneshian, Brendan Abiskaroon, Maksymilian Chruszcz, Edward L. D'Antonio
Summary: The importance of F337 in TcGlcK was investigated using the hole mutagenesis approach, revealing its key interactions with aromatic tail groups in inhibitor molecules. Mutants retaining activity and structural similarity were obtained, suggesting the possibility of TcHxK existing as a monomer lacking the F337 residue. Similar D-glucosamine inhibitors may bind less strongly to TcHxK due to the absence of van der Waals contacts from residue side chains.
Article
Biochemistry & Molecular Biology
Catia Barria, Diogo Athayde, Guillem Hernandez, Leonor Fonseca, Jorge Casinhas, Tiago N. Cordeiro, Margarida Archer, Cecilia M. Arraiano, Jose A. Brito, Rute G. Matos
Summary: Ribonucleases play a crucial role in RNA regulation by processing, degrading, and quality controlling cellular transcripts. Campylobacter jejuni, the main cause of human gastroenteritis, relies on the exoribonuclease PNPase (CjPNP) for low-temperature survival, virulence factor synthesis, and swimming, cell adhesion/invasion, and chick colonization abilities. The crystallographic structure of CjPNP, along with SAXS analysis, confirms its trimeric arrangement and provides insight into domain arrangement and flexibility. Mutations in highly conserved residues reveal unexpected RNA degradation activity even under conditions favoring polymerization. These findings have significant implications for the development of strategies to combat C. jejuni infections.
Article
Biochemistry & Molecular Biology
Elvira R. Shaykhutdinova, Maria S. Severyukhina, Inna V. Kholoshenko, Elena A. Gondarenko, Irina V. Shelukhina, Elena V. Kryukova, Alina M. Ismailova, Elena S. Sadovnikova, Igor A. Dyachenko, Arkady N. Murashev, Victor I. Tsetlin, Yuri N. Utkin
Summary: Evidence suggests that activating nicotinic acetylcholine receptors (nAChRs) can reduce cardiac injury caused by ischemia and reperfusion. In this study, the expression of a4, a6, and a132 nAChR subunits in the rat heart's left ventricle was demonstrated. The nAChR agonists cytisine and varenicline, commonly used for nicotine addiction treatment, were found to significantly reduce myocardial ischemia-reperfusion injury, with varenicline showing greater protection. The presence of a4132, a7, and/or a6132-containing receptors suggests that the protective effect of varenicline may be mediated by these subunits. These findings indicate a potential new use for cytisine and varenicline as cardioprotective agents.
Article
Biochemistry & Molecular Biology
Xinhai Xiong, Wenjie Chen, Cheng Chen, Qi Wu, Chaopeng He
Summary: This article summarizes the important roles of Cx43 in disease development from the perspective of subcellular localization and provides new ideas for Cx43 as a therapeutic target and the search for related pathological mechanisms.
Article
Biochemistry & Molecular Biology
Veronica Bastos, Simone Pascoal, Kamila Lopes, Marcia Mortari, Helena Oliveira
Summary: Cancer, especially melanoma, is a major global health problem due to its metastatic capacity, treatment resistance, and high mortality rates. The Chartergellus-CP1 peptide isolated from Chartergellus communis wasp venom showed selective cytotoxicity towards melanoma cell lines, inducing apoptosis, cell cycle impairment, and intracellular ROS increase.
Article
Biochemistry & Molecular Biology
Charlotte Claeyssen, Nathan Bulangalire, Bruno Bastide, Onnik Agbulut, Caroline Cieniewski-Bernard
Summary: Maintenance of striated muscle tissue relies on a dynamic network of protein-protein interactions. Desmin intermediate filaments play a crucial role in heart and skeletal muscle by maintaining cell structure and contributing to various cellular processes. The assembly of desmin intermediate filaments requires aB-crystallin, which is involved in multiple cellular functions.
Article
Biochemistry & Molecular Biology
Dhritiman Dey, Adhiraj Dasgupta, Dipanjan Ghosh, Oindrila Bhattacharjee, Abhrajyoti Ghosh, Ayae Honda, Dhrubajyoti Chattopadhyay
Summary: This study successfully identified host factors that interact with Chandipura Virus (CHPV) and play a critical role in infection, including A2M, LRP1, and GRP78. Knocking out A2M has a severe effect on viral infection, indicating the potential role of these host proteins in viral entry into host cells.
Article
Biochemistry & Molecular Biology
Anna Dotsenko, Igor Sinelnikov, Aleksandra Rozhkova, Ivan Zorov, Arkady Sinitsyn
Summary: Xylanases play important roles in various industrial applications, such as feed additives, pulp and paper bleaching, and the production of bread, food, and drinks. Xylanases from the GH10 family show high thermostability and resistance to protein inhibitors, making them promising candidates for these processes. This study successfully improved the thermostability and enzymatic activity of GH10 xylanase, which could enhance its effectiveness as a feed additive and during pulp and paper bleaching.
Article
Biochemistry & Molecular Biology
M. Jaquet, M. Bengue, K. Lambert, G. Carnac, D. Misse, C. Bisbal
Summary: Changes to our environment have led to the emergence of human pathogens such as chikungunya virus, which is now a major public health concern. In this study, the interconnection between skeletal muscle cells metabolism, their differentiation stage, and the infectivity of chikungunya virus was investigated. The results showed that chikungunya virus infectivity is dependent on cell differentiation and metabolism and that the virus interferes with cellular metabolism.
