Article
Multidisciplinary Sciences
Shaun K. Bremner, Rebecca Berends, Alexandra Kaupisch, Jennifer Roccisana, Calum Sutherland, Nia J. Bryant, Gwyn W. Gould
Summary: Insulin stimulates glucose transport by regulating the delivery of GLUT4-containing vesicles. Sx16 and mVps45 have been identified as key proteins involved in the delivery of GLUT4. Mutations in the binding sites of these proteins affect GLUT4 sorting, and phosphorylation of Sx16 at T7 is important for GLUT4 trafficking.
Article
Endocrinology & Metabolism
Erik A. Richter
Summary: Exercise in humans greatly increases muscle glucose uptake, but the translocation of GLUT4 to the cell membrane only doubles, indicating a significant disparity. This could be due to underestimated measurements or an increase in GLUT4 intrinsic activity during exercise.
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
(2021)
Article
Biochemistry & Molecular Biology
Yan-Bo Kou, Xiao-Qing Yan, Qi-Yue Jing, Sheng-Han Zhang, Zhuan-Zhuan Liu, Yan-Xia Wei, Yu-Gang Wang
Summary: The study showed that the LIGHT-LTβR-AKT-GLUT4 axis plays a crucial role in regulating glucose uptake in adipose tissue, highlighting the importance of LIGHT in maintaining glucose homeostasis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Endocrinology & Metabolism
Yosuke Masubuchi, Jinhui Ma, Tomohiro Suzuki, Itaru Kojima, Takeshi Inagaki, Hiroshi Shibata
Summary: T1R3 receptor plays a non-gustatory role in adipocytes by negatively regulating insulin action of glucose transport through G alpha s-dependent microtubule disassembly.
Article
Endocrinology & Metabolism
Belen Picatoste, Lucie Yammine, Rosemary A. Leahey, David Soares, Emma F. Johnson, Paul Cohen, Timothy E. McGraw
Summary: Deleting Rab10 from brown adipocytes reduces insulin-stimulated glucose transport, leading to whole-body metabolic imbalance and insulin resistance, particularly in female mice. This effect is independent of the thermogenic function of brown adipocytes, highlighting a metabolism-specific role in female mice.
MOLECULAR METABOLISM
(2021)
Article
Neurosciences
Stewart W. C. Masson, Jonathan S. T. Woodhead, Randall F. D'Souza, Sophie C. Broome, Caitlin MacRae, Hyun C. Cho, Robert D. Atiola, Tumanu Futi, Jessica R. Dent, Peter R. Shepherd, Troy L. Merry
Summary: Beta-catenin plays a crucial role in regulating glucose uptake during exercise/contraction, potentially through facilitating actin cytoskeleton remodelling to support glucose transport.
JOURNAL OF PHYSIOLOGY-LONDON
(2021)
Article
Endocrinology & Metabolism
Yan Zhang, Jing Cui, Ze Wang, Ying Zhu, Ningkun Zhang, Yu Chen
Summary: This study shows that Apelin stimulates GLUT4 translocation and improves glucose utilization through the AKt and eNOS pathways. Apelin can potentially enhance the mechanism of insulin resistance by mediating the inflammatory response in 3T3-L1 adipocytes.
JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS
(2023)
Article
Endocrinology & Metabolism
Yosuke Masubuchi, Jinhui Ma, Tomohiro Suzuki, Itaru Kojima, Takeshi Inagaki, Hiroshi Shibata
Summary: T1R3, expressed in adipocytes, negatively regulates insulin-induced glucose transport via Gαs-dependent microtubule disassembly. Activation of T1R3 also inhibits insulin-induced GLUT4 translocation to the plasma membrane.
Article
Medicine, Research & Experimental
Vitor F. Martins, Samuel A. LaBarge, Alexandra Stanley, Kristoffer Svensson, Chao-Wei Hung, Omer Keinan, Theodore P. Ciaraldi, Dion Banoian, Ji E. Park, Christina Ha, Byron Hetrick, Gretchen A. Meyer, Andrew Philp, Larry L. David, Robert R. Henry, Joseph E. Aslan, Alan R. Saltiel, Carrie. E. McCurdy, Simon Schenk
Summary: This study highlights the fundamental role of acetylation and p300/CBP in the direct regulation of insulin-stimulated glucose transport in skeletal muscle and adipocytes.
Article
Biochemistry & Molecular Biology
Jorge G. Garcia, Eduardo Ansorena, Fermin I. Milagro, Guillermo Zalba, Carlos de Miguel
Summary: This study suggests that endothelial NOX5 expression may regulate glucose sensitivity and lipid homeostasis in adipose tissue under conditions of high-calorie diet.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Shaun K. Bremner, Woroud S. Al Shammari, Roderick S. Milligan, Brian D. Hudson, Calum Sutherland, Nia J. Bryant, Gwyn W. Gould
Summary: Adipocytes have multiple roles in glucose metabolism regulation, and the regulation of membrane traffic is crucial for these functions. The protein Sx16 plays an important role in the insulin-regulated trafficking of GLUT4, and its deficiency can lead to reduced GLUT4 expression and translocation. Moreover, the loss of Sx16 also affects GLUT1 levels and alters glucose transport and metabolic profiles.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Fumitaka Kawakami, Motoki Imai, Yuki Isaka, Mark R. Cookson, Hiroko Maruyama, Makoto Kubo, Matthew J. Farrer, Makoto Kanzaki, Rei Kawashima, Tatsunori Maekawa, Shun Tamaki, Yoshifumi Kurosaki, Fumiaki Kojima, Kenichi Ohba, Takafumi Ichikawa
Summary: This study demonstrates the negative impact of LRRK2 on glucose tolerance in high-fat diet-induced mice. In adipose tissue, LRRK2 kinase activity suppresses Rab8 and Rab10-mediated GLUT4 membrane translocation, exacerbating glucose intolerance.
Article
Biochemistry & Molecular Biology
Beth A. Griesel, Satoshi Matsuzaki, Albert Batushansky, Timothy M. Griffin, Kenneth M. Humphries, Ann Louise Olson
Summary: This study using 3T3-L1 adipocytes as a model system found that an increase in glucose uptake during the first 3 days of differentiation promoted the expansion of the adipocyte metabolome and proteome. The positive effects were specifically due to increased expression of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3), highlighting its critical role in regulating cellular metabolic remodeling for adipocyte differentiation and maturation.
Article
Nutrition & Dietetics
Amy Zheng, Haiyan Wang, Edward B. Arias, Gengfu Dong, Jiahui Zhao, Gregory D. Cartee
Summary: The effects of calorie restriction on female wildtype and Akt substrate of 160 kDa knockout rats were evaluated. The results showed that calorie restriction increased muscle glucose uptake and GLUT4 protein abundance, and AS160 expression was essential for these increases.
APPLIED PHYSIOLOGY NUTRITION AND METABOLISM
(2023)
Article
Biochemistry & Molecular Biology
Kiko Hasegawa, Nobuyuki Takenaka, Kenya Tanida, Man Piu Chan, Mizuki Sakata, Atsu Aiba, Takaya Satoh
Summary: Rac1 is involved in insulin-dependent glucose uptake and lipogenesis in white adipocytes, and its deficiency may result in reduced insulin responsiveness and atrophy of white adipose tissues.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Letter
Multidisciplinary Sciences
Franck Peiretti, Rene Valero, Roland Govers
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Thermodynamics
Victor M. Nazarychev, Artyom D. Glova, Igor V. Volgin, Sergey Larin, Alexey Lyulin, Sergey Lyulin, Andrey A. Gurtovenko
Summary: This study investigates the effects of different atomistic force field models on the calculation of thermal conductivity of n-eicosane samples using molecular dynamics simulations. The results show significant differences in the performance of different models in crystalline and liquid states, highlighting the challenging task of selecting an appropriate model for thermal conductivity calculations.
INTERNATIONAL JOURNAL OF HEAT AND MASS TRANSFER
(2021)
Article
Plant Sciences
Farah Drissi, Farid Lahfa, Teresa Gonzalez, Franck Peiretti, Jean-Francois Tanti, Mohamed Haddad, Nicolas Fabre, Roland Govers
Summary: The study shows that Citrullus colocynthis fruit has insulin-enhancing effects, which may partly explain its antidiabetic effects in traditional medicine.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Benedicte Gaborit, Roland Govers, Alexandre Altie, Jean Michel Brunel, Pierre Morange, Franck Peiretti
Summary: During diabetes, BACE1 cleavage of the insulin receptor increases in the liver, affecting insulin signaling. The proform of BACE1 interacts with the insulin receptor in the early secretory pathway, and its accumulation is regulated by O-GlcNacylation and degradation systems. Claramine accelerates intracellular trafficking of BACE1, reducing insulin receptor cleavage by increasing autophagy.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Chemistry, Physical
Artyom D. Glova, Victor M. Nazarychev, Sergey Larin, Alexey Lyulin, Sergey Lyulin, Andrey A. Gurtovenko
Summary: This study explores the potential of chemically modified asphaltene molecules as thermal conductivity enhancers for liquid paraffin, showing promising results for improving the thermal energy storage systems based on paraffin.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Food Science & Technology
Tiffany Antoine, Patrick Borel, Roland Govers, Laure Meiller, Philippe Guichard, Charlotte Halimi, Teresa Gonzalez, Marion Nowicki, Valerie Sauvinet, Michel Grino, Emmanuelle Reboul
Summary: This study investigated the effects of vitamin A deficiency on vitamin A and lipid postprandial metabolism in young rats, showing that vitamin A deficiency can modulate both vitamin A absorption rate and lipid postprandial metabolism, partially explaining the altered fasting lipid status observed in VAD diet-fed offspring.
MOLECULAR NUTRITION & FOOD RESEARCH
(2021)
Article
Chemistry, Physical
S. V. Larin, V. V. Makarova, S. N. Gorbacheva, M. R. Yakubov, S. V. Antonov, N. I. Borzdun, A. D. Glova, V. M. Nazarychev, A. A. Gurtovenko, S. V. Lyulin
Summary: In this study, polymer additive P3HT was added to paraffin-carbon nanoparticle blends to improve their sedimentation stability, reduce the size of nanoparticle aggregates, and increase the viscosity of the blends. The presence of P3HT had almost no effect on the thermophysical properties of the blends, preserving their critical properties for heat storage applications.
JOURNAL OF CHEMICAL PHYSICS
(2022)
Article
Biophysics
Matthew Davies, A. D. Reyes-Figueroa, Andrey A. Gurtovenko, Daniel Frankel, Mikko Karttunen
Summary: A new method combining mixed radial-angular, three-particle correlation function and unsupervised machine learning was used to investigate the emergence of the ripple phase in DPPC lipid bilayers. Data from atomistic molecular dynamics simulations of varying system sizes were analyzed, revealing four distinct conformational populations of lipids. The presence of ordered, disordered, and splayed tail lipids was observed, with spatial clustering of disordered lipids in the groove side of the ripple phase. Principal component analysis confirmed the existence of the four lipid groups.
BIOPHYSICAL JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Victor M. Nazarychev, Artyom D. Glova, Sergey V. Larin, Alexey V. Lyulin, Sergey V. Lyulin, Andrey A. Gurtovenko
Summary: An insight into the impact of cooling rate on phase transformations in molecular systems was gained through computational simulations on organic phase-change materials. It was found that a certain threshold in cooling rates exists, below which the simulations qualitatively reproduced experimental observations, and beyond which the simulations started to deviate considerably from experimental data.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Physical
Andrey A. A. Gurtovenko, Victor M. M. Nazarychev, Artem D. D. Glova, Sergey V. V. Larin, Sergey V. V. Lyulin
Summary: In this study, a coarse-grained Martini model was developed to simulate the aggregation behavior of asphaltenes. It was found that native asphaltenes form small clusters that are uniformly distributed in the system, while modified asphaltenes form larger aggregates. Additionally, the mobility of native asphaltenes is slower and the size of aggregates increases with system size.
JOURNAL OF CHEMICAL PHYSICS
(2023)
Article
Chemistry, Physical
Artem D. Glova, Victor M. Nazarychev, Sergey V. Larin, Andrey A. Gurtovenko, Sergey V. Lyulin
Summary: Atomistic computer simulations suggest that asphaltenes with enlarged aromatic cores can improve the performance of heat storage devices based on organic phase change materials. Increasing the size of the asphaltene cores promotes the formation of extended structures in paraffin and enhances the thermal conductivity of liquid paraffin.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Chemistry, Physical
Andrey A. Gurtovenko, Mikko Karttunen
Summary: This study investigated the interactions between cellulose crystals and model stratum corneum bilayers using molecular dynamics simulations. The findings highlight the critical role of fatty acid protonation in controlling the binding between cellulose and skin.
Correction
Chemistry, Multidisciplinary
Igor V. Volgin, Artyom D. Glova, Victor M. Nazarychev, Sergey V. Larin, Sergey V. Lyulin, Andrey A. Gurtovenko
Article
Biochemistry & Molecular Biology
Siva Santhiya Arul, Brinda Balakrishnan, Savithri S. Handanahal, Sangita Venkataraman
Summary: Viral nanoparticles are self-assembling delivery systems used for vaccines and therapeutic agents. They have improved biocompatibility and are widely used in various biomedical applications. However, large-scale production of VNPs still faces challenges.
Article
Biochemistry & Molecular Biology
Wisely Chua, Carl O. Marsh, Si En Poh, Winston LC. Koh, Melody Li Ying Lee, Li Fang Koh, Xin-Zi Emily Tang, Peter See, Zheng Ser, Shi Mei Wang, Radoslaw M. Sobota, Thomas L. Dawson Jr, Yik Weng Yew, Steven Thng, Anthony J. O'Donoghue, Hazel H. Oon, John E. Common, Hao Li
Summary: In this study, the gene expression of enzymes secreted by Malassezia globosa in the skin was investigated using targeted RNA sequencing. The study found that the expression of these enzymes is regulated by the fungus's environment and differs significantly from healthy skin sites. Additionally, a pseudoprotease MGL_3331 was identified to elicit an immune reaction in patients with atopic dermatitis. This highlights the importance of studying fungal proteins in physiologically relevant environments and their role in host immunity.
Article
Biochemistry & Molecular Biology
Belisa Parmeggiani, Marian Flores Signori, Cristiane Cecatto, Marina Rocha Frusciante, Manuela Bianchin Marcuzzo, Debora Guerini Souza, Rafael Teixeira Ribeiro, Bianca Seminotti, Diogo Onofre Gomes de Souza, Cesar Augusto Joa o Ribeiro, Moacir Wajner, Guilhian Leipnitz
Summary: Non ketotic hyperglycinemia (NKH) is a disorder caused by mutations in the genes encoding glycine cleavage system proteins, leading to severe neurodegeneration and white matter alterations. The study found that glycine affects myelin structure, glutamatergic system, and induces oxidative stress in neonatal rats and MO3.13 oligodendroglial cells.
Review
Biochemistry & Molecular Biology
Marie Rose Ndella Thiaw, Pascal Gantet
Summary: Mini zinc fingers play important roles in plant development, including control of cell division and expansion, meristem state transition, and growth and organ development. They have diverse modes of action, such as direct gene activation, interaction with specific transcription factors, and aggregation of protein complexes for chromatin remodeling. Further research is needed to explore their wider range of biological functions and their involvement in biotic and abiotic stress responses.
Article
Biochemistry & Molecular Biology
Shao-Jui Lai, Takahiro Kameda, Maasa Morita, Yuka Yamagata, Kaoruko Nishizaka, Yuna Horiuchi, Yukihiro Kobayashi, Yoko Usami, Jun-Jen Liu, Takeshi Kasama, Minoru Tozuka, Ryunosuke Ohkawa
Summary: In this study, the co-modification and impact of chymase and MPO on HDL were investigated. Sequential treatment with MPO and chymase generated two novel apoA-I fragments from HDL, one of which has a specific immunological property and can be used to predict patients with normal HDL-C levels and cardiovascular diseases.
Article
Biochemistry & Molecular Biology
Xiao-Mi Sun, Asami Yoshida, Takuya Ishii, Yan-Rong Jiang, Yi-Li Gao, Mikinori Ueno, Katsuya Hirasaka, Kiyoshi Osatomi
Summary: This study found that infection with the causative bacteria of edwardsiellosis, Edwardsiella tarda NUF251, leads to the production of superoxide radicals, causing oxidative stress. The research on the Jfsod1 gene suggests that the transcription factor NF-IL6 plays a significant role in upregulating the transcriptional activity of the gene in response to oxidative stress induced by NUF251 infection.
Article
Biochemistry & Molecular Biology
Shane M. Carey, Sean P. Kearns, Matthew E. Millington, Gregory S. Buechner, Beda E. Alvarez Jr, Leily Daneshian, Brendan Abiskaroon, Maksymilian Chruszcz, Edward L. D'Antonio
Summary: The importance of F337 in TcGlcK was investigated using the hole mutagenesis approach, revealing its key interactions with aromatic tail groups in inhibitor molecules. Mutants retaining activity and structural similarity were obtained, suggesting the possibility of TcHxK existing as a monomer lacking the F337 residue. Similar D-glucosamine inhibitors may bind less strongly to TcHxK due to the absence of van der Waals contacts from residue side chains.
Article
Biochemistry & Molecular Biology
Catia Barria, Diogo Athayde, Guillem Hernandez, Leonor Fonseca, Jorge Casinhas, Tiago N. Cordeiro, Margarida Archer, Cecilia M. Arraiano, Jose A. Brito, Rute G. Matos
Summary: Ribonucleases play a crucial role in RNA regulation by processing, degrading, and quality controlling cellular transcripts. Campylobacter jejuni, the main cause of human gastroenteritis, relies on the exoribonuclease PNPase (CjPNP) for low-temperature survival, virulence factor synthesis, and swimming, cell adhesion/invasion, and chick colonization abilities. The crystallographic structure of CjPNP, along with SAXS analysis, confirms its trimeric arrangement and provides insight into domain arrangement and flexibility. Mutations in highly conserved residues reveal unexpected RNA degradation activity even under conditions favoring polymerization. These findings have significant implications for the development of strategies to combat C. jejuni infections.
Article
Biochemistry & Molecular Biology
Elvira R. Shaykhutdinova, Maria S. Severyukhina, Inna V. Kholoshenko, Elena A. Gondarenko, Irina V. Shelukhina, Elena V. Kryukova, Alina M. Ismailova, Elena S. Sadovnikova, Igor A. Dyachenko, Arkady N. Murashev, Victor I. Tsetlin, Yuri N. Utkin
Summary: Evidence suggests that activating nicotinic acetylcholine receptors (nAChRs) can reduce cardiac injury caused by ischemia and reperfusion. In this study, the expression of a4, a6, and a132 nAChR subunits in the rat heart's left ventricle was demonstrated. The nAChR agonists cytisine and varenicline, commonly used for nicotine addiction treatment, were found to significantly reduce myocardial ischemia-reperfusion injury, with varenicline showing greater protection. The presence of a4132, a7, and/or a6132-containing receptors suggests that the protective effect of varenicline may be mediated by these subunits. These findings indicate a potential new use for cytisine and varenicline as cardioprotective agents.
Article
Biochemistry & Molecular Biology
Xinhai Xiong, Wenjie Chen, Cheng Chen, Qi Wu, Chaopeng He
Summary: This article summarizes the important roles of Cx43 in disease development from the perspective of subcellular localization and provides new ideas for Cx43 as a therapeutic target and the search for related pathological mechanisms.
Article
Biochemistry & Molecular Biology
Veronica Bastos, Simone Pascoal, Kamila Lopes, Marcia Mortari, Helena Oliveira
Summary: Cancer, especially melanoma, is a major global health problem due to its metastatic capacity, treatment resistance, and high mortality rates. The Chartergellus-CP1 peptide isolated from Chartergellus communis wasp venom showed selective cytotoxicity towards melanoma cell lines, inducing apoptosis, cell cycle impairment, and intracellular ROS increase.
Article
Biochemistry & Molecular Biology
Charlotte Claeyssen, Nathan Bulangalire, Bruno Bastide, Onnik Agbulut, Caroline Cieniewski-Bernard
Summary: Maintenance of striated muscle tissue relies on a dynamic network of protein-protein interactions. Desmin intermediate filaments play a crucial role in heart and skeletal muscle by maintaining cell structure and contributing to various cellular processes. The assembly of desmin intermediate filaments requires aB-crystallin, which is involved in multiple cellular functions.
Article
Biochemistry & Molecular Biology
Dhritiman Dey, Adhiraj Dasgupta, Dipanjan Ghosh, Oindrila Bhattacharjee, Abhrajyoti Ghosh, Ayae Honda, Dhrubajyoti Chattopadhyay
Summary: This study successfully identified host factors that interact with Chandipura Virus (CHPV) and play a critical role in infection, including A2M, LRP1, and GRP78. Knocking out A2M has a severe effect on viral infection, indicating the potential role of these host proteins in viral entry into host cells.
Article
Biochemistry & Molecular Biology
Anna Dotsenko, Igor Sinelnikov, Aleksandra Rozhkova, Ivan Zorov, Arkady Sinitsyn
Summary: Xylanases play important roles in various industrial applications, such as feed additives, pulp and paper bleaching, and the production of bread, food, and drinks. Xylanases from the GH10 family show high thermostability and resistance to protein inhibitors, making them promising candidates for these processes. This study successfully improved the thermostability and enzymatic activity of GH10 xylanase, which could enhance its effectiveness as a feed additive and during pulp and paper bleaching.
Article
Biochemistry & Molecular Biology
M. Jaquet, M. Bengue, K. Lambert, G. Carnac, D. Misse, C. Bisbal
Summary: Changes to our environment have led to the emergence of human pathogens such as chikungunya virus, which is now a major public health concern. In this study, the interconnection between skeletal muscle cells metabolism, their differentiation stage, and the infectivity of chikungunya virus was investigated. The results showed that chikungunya virus infectivity is dependent on cell differentiation and metabolism and that the virus interferes with cellular metabolism.