Review
Agricultural Engineering
Hongliang Guo, Ying Zhao, Jo-Shu Chang, Duu-Jong Lee
Summary: Enzymatic hydrolysis is crucial for efficient biorefinery of lignocellulosic biomass. Understanding the properties of key enzymes and their interactions with biomass is essential for improving the process efficiency. This article provides an update on the research of key enzymes in lignocellulose biorefinery, including their properties, interactions with biomass, adsorption, and hydrolysis. The advanced analytical techniques for investigating these interactions and future research prospects are discussed.
BIORESOURCE TECHNOLOGY
(2023)
Article
Agricultural Engineering
Misa Mojca Cajnko, Jost Oblak, Miha Grilc, Blaz Likozar
Summary: Enzymes play a crucial role in lignin valorization, with different enzymes involved in various elementary reaction mechanisms. Degradation of lignin depends on enzyme activity and the interaction with proteins in ways that target specific covalent bonds in lignin. Various factors influence enzymatic activity, which must be taken into consideration for optimal functionality and synthesis yields.
BIORESOURCE TECHNOLOGY
(2021)
Article
Engineering, Environmental
Ramin Bahmani, Mahsa Modareszadeh, DongGwan Kim, Seongbin Hwang
Summary: The VIM1 gene, which regulates DNA methylation, was found to enhance arsenic tolerance and reduce arsenic accumulation in Arabidopsis and yeast. VIM1 increases DNA methylation at the promoter of the As(III) importer gene NIP3;1, leading to decreased expression of NIP3;1 and reduced arsenic uptake.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Review
Engineering, Chemical
Peng Dong, Tingna Shao, Yu Zhao, Dong Ji, Yong Yang, Xinhong Zhao, Hongwei Li, Junying Tian, Dongliang Wang, Guixian Li
Summary: The high-efficiency production of xylene/trimethylbenzene from benzene/toluene with methanol is a potential way to optimize the resource structure and promote the development of the petrochemical and coal chemical industry. This paper focuses on the one-step methylation reaction and reviews the mechanism and preparation strategy of high-performance catalysts. It also describes the current research progress and future directions in this field.
Article
Engineering, Environmental
Jun Dai, Chuan Chen, A-Xiang Gao, Zhu Tang, Peter M. Kopittke, Fang-Jie Zhao, Peng Wang
Summary: The transformation of arsenic species in paddy soils has significant implications for the accumulation of arsenic in rice grains and its safety to consumers. The study found that DMMTA is the main methylated thioarsenate species in both flooded soils and paddy fields, accumulating in rice grains and increasing the risk to food safety and human health.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Food Science & Technology
Chenwei Zhao, Pengkai Xie, Jun Jin, Qingzhe Jin, Xingguo Wang
Summary: The kinetics and thermodynamics of enzymatic degradation of zearalenone (ZEN) in degummed corn oil were studied. The maximum reaction rate was found to be 0.97 µmol·kg(-1)·min(-1), the Michaelis constant (Km) was 11,476 µmol·kg(-1) and the reaction followed the Michaelis equation V = 0.97[S]/(11,476 + [S]). The thermodynamic study showed that the reaction had a positive activation energy (Ea), positive activation enthalpy change (Delta H), positive free energy of activation (Delta G), and negative activation entropy change (Delta S), indicating a non-spontaneous reaction. The reaction mechanism of ZEN involved the generation of intermediate G/L/D/W-ZEN+H2O and W-ZEN-H2O, followed by the opening of the lactone bond to produce C18H24O6, and finally the automatic formation of decarboxylation product C17H24O4.
Article
Biophysics
Emery T. Usher, Kevin E. W. Namitz, Michael S. Cosgrove, Scott A. Showalter
Summary: Post-translational modification, particularly methylation, plays a critical role in regulating cellular processes. By studying methyltransferase function through in vitro experiments, researchers can gain insight into the complex mechanisms of regulated gene expression. The use of two-dimensional NMR to monitor methylation events shows promise in understanding the intricate processes of methyltransferases and other enzymes.
BIOPHYSICAL JOURNAL
(2021)
Article
Chemistry, Physical
Diana A. Amariei, Nadiia Pozhydaieva, Benoit David, Pascal Schneider, Thomas Classen, Holger Gohlke, Oliver H. Weiergraeber, Joerg Pietruszka
Summary: Enantioselective methylation is a challenging task in organic chemistry. The study found that S-Adenosyl methionine (SAM) dependent methyltransferases (MTases) could serve as a selective alternative with abundant potential applications.
Article
Engineering, Environmental
Leila Alidokht, Ioannis Anastopoulos, Dimitrios Ntarlagiannis, Pantelis Soupios, Bassam Tawabini, Dimitrios Kalderis, Alireza Khataee
Summary: This review focuses on the recent advances in the use of nanotechnologies and nanocomposites for remediation of arsenic-contaminated water and soil. Various engineered organic/inorganic nanocomposites with exceptional physical-chemical properties have been developed, showing great potential for As removal or immobilization in contaminated sites. Emerging approaches include the development of bio-nanocomposites with both oxidative and adsorptive capacities, while gaps in research and directions for future studies have been identified.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Environmental Sciences
Xin Tang, Jingyu Wen, Li Mu, Ziwei Gao, Jingxian Weng, Xiaokang Li, Xiangang Hu
Summary: This study examined the interaction between pyrite and glutamic acid in arsenite solution, evaluated the influence of pyrite and glutamic acid on arsenite plant toxicity, and clarified the metabolic regulation mechanism of pyrite and glutamic acid on the arsenite phytotoxic effect through metabolomics analysis.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Chemistry, Physical
Paulo Roberto Garces Goncalves Jr, Heitor Avelino De Abreu, Helio Anderson Duarte
Summary: This study investigates the adsorption mechanism and oxidation process of arsenite and arsenate on hausmannite surfaces using computational methods. Different adsorption sites and the presence of water are found to have a significant impact on the reaction. The findings are important for mitigating the environmental impact of arsenic-rich tailings.
APPLIED SURFACE SCIENCE
(2022)
Article
Nutrition & Dietetics
Liang Feng, Yan Yang, Ya-ting Xie, Shuang-shuang Liu, Xuan Peng, Sheng Hu, Ai-nong Yu
Summary: The study investigated the degradation of L-ascorbic acid (ASA) and the generation of browning products (BPs) in hot-compressed water (HCW) by adjusting the reaction conditions. It was found that ASA degradation followed pseudo-first-order kinetics and the formation of BPs was closely related to the concentration of H3O+ in HCW. The acidic condition (pH=5.0) and lower concentration of ASA (0.03 mol/L) were found to be more favorable for ASA degradation.
FRONTIERS IN NUTRITION
(2023)
Article
Multidisciplinary Sciences
Giacomo Lancia, Claudio Durastanti, Cristian Spitoni, Ilaria De Benedictis, Antonio Sciortino, Emilio N. M. Cirillo, Mario Ledda, Antonella Lisi, Annalisa Convertino, Valentina Mussi
Summary: We use Surface Enhanced Raman Scattering (SERS) platform to discriminate genomic DNA of different subtypes of melanoma and colon tumors. This is crucial for personalized therapy guidance and predicting tumor metastasis ability.
SCIENTIFIC REPORTS
(2023)
Article
Environmental Sciences
Ankita Das, Tamalika Sanyal, Pritha Bhattacharjee
Summary: The SAM levels are reduced in individuals with arsenic induced skin lesions, and key regulatory genes in the SAM biogenesis pathway are significantly down-regulated in this group. Hypermethylation of the AS3MT promoter leads to its transcriptional repression, potentially contributing to the higher susceptibility in arsenic exposed individuals.
ENVIRONMENTAL RESEARCH
(2021)
Review
Chemistry, Multidisciplinary
Karel Asselman, Christine Kirschhock, Eric Breynaert
Summary: Since the 1940s, researchers have been aiming to rationally design zeolites by controlling their synthesis to obtain desired properties. However, the fundamental processes of zeolite synthesis have remained largely unknown. This Account presents recent progress in crystallizing zeolites using hydrated silicate ionic liquids as synthesis media, allowing for the evaluation of the individual impacts of synthesis parameters on zeolite nucleation, growth, and phase selection. The relationship between the characteristics of the synthesis medium and the properties and structure of the crystalline product is elucidated.
ACCOUNTS OF CHEMICAL RESEARCH
(2023)
Article
Medicine, Research & Experimental
Chunxia Chen, Shihai Yan, Shuang Qiu, Zhirong Geng, Zhilin Wang
Summary: The study demonstrated that FG4592 promotes the proliferation and migration of BMSCs, accelerating fracture healing. Additionally, HIF-1 alpha plays a crucial role in this process.
Article
Pharmacology & Pharmacy
Liu Shun, Wang Yi-xuan, Wang Hai-dan, Geng Zhi-rong, Yan Shi-hai
Summary: This study established a method to detect seven effective components in SKTM simultaneously using UHPLC-MS/MS, which was validated to have good repeatability and accuracy. This method may provide a new reliable approach for quality control of SKTM.
JOURNAL OF PHARMACY AND PHARMACOLOGY
(2021)
Article
Chemistry, Analytical
Taiyu Guo, Xinyue Chen, Wangbo Qu, Bin Yang, Ruowei Tian, Zhirong Geng, Zhilin Wang
Summary: A novel fluorescent probe, CySI, was developed to simultaneously distinguish cysteine and homocysteine with excellent selectivity and fast response. This probe could visualize exogenous Cys and Hcy in cells and monitor endogenous Cys fluctuations independently in mitochondria through the red emission channel.
ANALYTICAL CHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Chunxia Chen, Shihai Yan, Zhirong Geng, Zhilin Wang
Summary: The HIF-1 alpha PHD inhibitor IOX2 improves the proliferation and migration of BMSCs by upregulating intracellular Ca2+ and decreasing ROS, thus promoting fracture healing.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Plant Sciences
Shihai Yan, Jiayin Chang, Xuehui Hao, Jiang Liu, Xiying Tan, Zhirong Geng, Zhilin Wang
Summary: The study found that berberine can alleviate the occurrence of colitis-associated colorectal tumors by altering the composition of intestinal flora. Berberine increases beneficial bacteria and levels of short-chain fatty acids, reducing inflammation and the risk of cancer.
Article
Multidisciplinary Sciences
Shi-Hai Yan, Li-Mu Hu, Xue-Hui Hao, Jiang Liu, Xi-Ying Tan, Zhi-Rong Geng, Jing Ma, Zhi-Lin Wang
Summary: This study revealed the direct binding target protein of berberine in colorectal cancer cells, which is pyruvate kinase isozyme type M2 (PKM2). Berberine inhibits the progression of colorectal cancer through hydrophobic interaction and pi-pi interaction, and it also inhibits the reprogramming of glucose metabolism.
Article
Chemistry, Applied
Taiyu Guo, Ruowei Tian, Wangbo Qu, Bin Yang, Zhirong Geng, Zhilin Wang
Summary: A mitochondria-targeted turn-on fluorescent sensor, CySH, with near-infrared absorption and emission wavelengths, was developed for the selective determination of copper, showing high selectivity and excellent anti-interference ability.
Article
Biochemistry & Molecular Biology
Tianxiang Li, Zhirong Geng, Ju Zhang, Lu Xu, Xiaoli Zhu
Summary: This study demonstrates the protective effects of pentapeptide bursopentin (BP5) against endotoxemia-induced acute lung injury. BP5 effectively alleviates lung injury by reducing oxidative stress and excessive inflammatory response through activation of the Nrf2 pathway. This mechanism involves the regulation of Keap1-Nrf2 interaction and Akt/GSK3 beta/Fyn pathway.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Spectroscopy
Wangbo Qu, Taiyu Guo, Bin Yang, Ruowei Tian, Shuang Qiu, Xinyue Chen, Zhirong Geng, Zhilin Wang
Summary: DFSN is a fluorescent probe with AIE and ESIPT, which can efficiently track hypochlorous acid in vivo. The probe has good selectivity and sensitivity, and can detect hypochlorous acid with the naked eye.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Analytical
Wangbo Qu, Bin Yang, Taiyu Guo, Ruowei Tian, Shuang Qiu, Xinyue Chen, Zhirong Geng, Zhilin Wang
Summary: In this study, a dual-response mitochondria-targeted fluorescent probe HBTN was developed for the quantitative detection of viscosity and HOCl. The probe showed promising results in both in vitro and in vivo experiments, providing a practical tool for monitoring viscosity and HOCl.
Editorial Material
Pharmacology & Pharmacy
Qiuwang Zhang, Shihai Yan, Dongdong Sun, Zhirong Geng, Peixiang Zhang
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Chemistry, Analytical
Bin Yang, Ruowei Tian, Taiyu Guo, Wangbo Qu, Jiao Lu, Yong Li, Zhou Wu, Shihai Yan, Zhirong Geng, Zhilin Wang
Summary: This study reports the latest progress in using tetraphenylethylene (TPE) fluorophores as a tool for detecting intracellular adenosine triphosphate (ATP). Six different ATP probes were synthesized, and probes with dual positive charge sites showed better selectivity and sensitivity. Among them, P2, with a D-π-A structure, 1,4-bis(bromomethyl)benzene linker, and dual positive charge recognition sites, exhibited higher sensitivity and selectivity in ATP detection. This research has important application value in monitoring the fluctuations of mitochondrial ATP levels.
ANALYTICAL CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Hong Huang, Junyao Xu, Siqi Zhang, Jing Zhao, Shun Liu, Lei Tian, Haidan Wang, Zhirong Geng, Shihai Yan
Summary: This study used network pharmacology combined with UHPLC-MS/MS, molecular docking, and in vivo validation to investigate the active components and therapeutic mechanisms of SKTMG in the treatment of heart failure. The results identified 192 active compounds and 307 potential consensus targets for SKTMG, and discovered 10 core target genes related to the MAPK signal pathway. Additionally, SKTMG components were found to bind to specific targets and inhibit phosphorylation, leading to improved CHF in rats.
JOURNAL OF PHARMACY AND PHARMACOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Hai-Li Yang, Li-Fei Bai, Zhi-Rong Geng, Hao Chen, Le-Tian Xu, Yang-Cun Xie, Dong-Jin Wang, Hong-Wei Gu, Xiao-Ming Wang
Summary: As an excellent fluorescent nanomaterial, carbon quantum dots (CQDs) possess various properties that make them suitable for biomedical applications. This review provides an overview of the synthesis methods, optical properties, and biomedical applications of CQDs, including detection, imaging, and drug delivery. The review also discusses the challenges and future prospects of CQDs in the biomedical sector.
MATERIALS TODAY ADVANCES
(2023)
Article
Chemistry, Applied
Bin Yang, Wangbo Qu, Taiyu Guo, Ruowei Tian, Shuang Qiu, Xinyue Chen, Zhirong Geng, Zhilin Wang
Summary: A tetraphenylethylene fluorophore based on D-pi-A structure was used to design and synthesize the TPE-PyFP probe for selective detection of ATP. TPE-Py-FP showed a turn-on response after adding ATP and had a wide linear range and low limit of detection. The detection mechanism was mainly the electrostatic interaction between the positively charged sites of TPE-Py-FP and the negatively charged triphosphate structure of ATP.
Article
Biochemistry & Molecular Biology
Siva Santhiya Arul, Brinda Balakrishnan, Savithri S. Handanahal, Sangita Venkataraman
Summary: Viral nanoparticles are self-assembling delivery systems used for vaccines and therapeutic agents. They have improved biocompatibility and are widely used in various biomedical applications. However, large-scale production of VNPs still faces challenges.
Article
Biochemistry & Molecular Biology
Wisely Chua, Carl O. Marsh, Si En Poh, Winston LC. Koh, Melody Li Ying Lee, Li Fang Koh, Xin-Zi Emily Tang, Peter See, Zheng Ser, Shi Mei Wang, Radoslaw M. Sobota, Thomas L. Dawson Jr, Yik Weng Yew, Steven Thng, Anthony J. O'Donoghue, Hazel H. Oon, John E. Common, Hao Li
Summary: In this study, the gene expression of enzymes secreted by Malassezia globosa in the skin was investigated using targeted RNA sequencing. The study found that the expression of these enzymes is regulated by the fungus's environment and differs significantly from healthy skin sites. Additionally, a pseudoprotease MGL_3331 was identified to elicit an immune reaction in patients with atopic dermatitis. This highlights the importance of studying fungal proteins in physiologically relevant environments and their role in host immunity.
Article
Biochemistry & Molecular Biology
Belisa Parmeggiani, Marian Flores Signori, Cristiane Cecatto, Marina Rocha Frusciante, Manuela Bianchin Marcuzzo, Debora Guerini Souza, Rafael Teixeira Ribeiro, Bianca Seminotti, Diogo Onofre Gomes de Souza, Cesar Augusto Joa o Ribeiro, Moacir Wajner, Guilhian Leipnitz
Summary: Non ketotic hyperglycinemia (NKH) is a disorder caused by mutations in the genes encoding glycine cleavage system proteins, leading to severe neurodegeneration and white matter alterations. The study found that glycine affects myelin structure, glutamatergic system, and induces oxidative stress in neonatal rats and MO3.13 oligodendroglial cells.
Review
Biochemistry & Molecular Biology
Marie Rose Ndella Thiaw, Pascal Gantet
Summary: Mini zinc fingers play important roles in plant development, including control of cell division and expansion, meristem state transition, and growth and organ development. They have diverse modes of action, such as direct gene activation, interaction with specific transcription factors, and aggregation of protein complexes for chromatin remodeling. Further research is needed to explore their wider range of biological functions and their involvement in biotic and abiotic stress responses.
Article
Biochemistry & Molecular Biology
Shao-Jui Lai, Takahiro Kameda, Maasa Morita, Yuka Yamagata, Kaoruko Nishizaka, Yuna Horiuchi, Yukihiro Kobayashi, Yoko Usami, Jun-Jen Liu, Takeshi Kasama, Minoru Tozuka, Ryunosuke Ohkawa
Summary: In this study, the co-modification and impact of chymase and MPO on HDL were investigated. Sequential treatment with MPO and chymase generated two novel apoA-I fragments from HDL, one of which has a specific immunological property and can be used to predict patients with normal HDL-C levels and cardiovascular diseases.
Article
Biochemistry & Molecular Biology
Xiao-Mi Sun, Asami Yoshida, Takuya Ishii, Yan-Rong Jiang, Yi-Li Gao, Mikinori Ueno, Katsuya Hirasaka, Kiyoshi Osatomi
Summary: This study found that infection with the causative bacteria of edwardsiellosis, Edwardsiella tarda NUF251, leads to the production of superoxide radicals, causing oxidative stress. The research on the Jfsod1 gene suggests that the transcription factor NF-IL6 plays a significant role in upregulating the transcriptional activity of the gene in response to oxidative stress induced by NUF251 infection.
Article
Biochemistry & Molecular Biology
Shane M. Carey, Sean P. Kearns, Matthew E. Millington, Gregory S. Buechner, Beda E. Alvarez Jr, Leily Daneshian, Brendan Abiskaroon, Maksymilian Chruszcz, Edward L. D'Antonio
Summary: The importance of F337 in TcGlcK was investigated using the hole mutagenesis approach, revealing its key interactions with aromatic tail groups in inhibitor molecules. Mutants retaining activity and structural similarity were obtained, suggesting the possibility of TcHxK existing as a monomer lacking the F337 residue. Similar D-glucosamine inhibitors may bind less strongly to TcHxK due to the absence of van der Waals contacts from residue side chains.
Article
Biochemistry & Molecular Biology
Catia Barria, Diogo Athayde, Guillem Hernandez, Leonor Fonseca, Jorge Casinhas, Tiago N. Cordeiro, Margarida Archer, Cecilia M. Arraiano, Jose A. Brito, Rute G. Matos
Summary: Ribonucleases play a crucial role in RNA regulation by processing, degrading, and quality controlling cellular transcripts. Campylobacter jejuni, the main cause of human gastroenteritis, relies on the exoribonuclease PNPase (CjPNP) for low-temperature survival, virulence factor synthesis, and swimming, cell adhesion/invasion, and chick colonization abilities. The crystallographic structure of CjPNP, along with SAXS analysis, confirms its trimeric arrangement and provides insight into domain arrangement and flexibility. Mutations in highly conserved residues reveal unexpected RNA degradation activity even under conditions favoring polymerization. These findings have significant implications for the development of strategies to combat C. jejuni infections.
Article
Biochemistry & Molecular Biology
Elvira R. Shaykhutdinova, Maria S. Severyukhina, Inna V. Kholoshenko, Elena A. Gondarenko, Irina V. Shelukhina, Elena V. Kryukova, Alina M. Ismailova, Elena S. Sadovnikova, Igor A. Dyachenko, Arkady N. Murashev, Victor I. Tsetlin, Yuri N. Utkin
Summary: Evidence suggests that activating nicotinic acetylcholine receptors (nAChRs) can reduce cardiac injury caused by ischemia and reperfusion. In this study, the expression of a4, a6, and a132 nAChR subunits in the rat heart's left ventricle was demonstrated. The nAChR agonists cytisine and varenicline, commonly used for nicotine addiction treatment, were found to significantly reduce myocardial ischemia-reperfusion injury, with varenicline showing greater protection. The presence of a4132, a7, and/or a6132-containing receptors suggests that the protective effect of varenicline may be mediated by these subunits. These findings indicate a potential new use for cytisine and varenicline as cardioprotective agents.
Article
Biochemistry & Molecular Biology
Xinhai Xiong, Wenjie Chen, Cheng Chen, Qi Wu, Chaopeng He
Summary: This article summarizes the important roles of Cx43 in disease development from the perspective of subcellular localization and provides new ideas for Cx43 as a therapeutic target and the search for related pathological mechanisms.
Article
Biochemistry & Molecular Biology
Veronica Bastos, Simone Pascoal, Kamila Lopes, Marcia Mortari, Helena Oliveira
Summary: Cancer, especially melanoma, is a major global health problem due to its metastatic capacity, treatment resistance, and high mortality rates. The Chartergellus-CP1 peptide isolated from Chartergellus communis wasp venom showed selective cytotoxicity towards melanoma cell lines, inducing apoptosis, cell cycle impairment, and intracellular ROS increase.
Article
Biochemistry & Molecular Biology
Charlotte Claeyssen, Nathan Bulangalire, Bruno Bastide, Onnik Agbulut, Caroline Cieniewski-Bernard
Summary: Maintenance of striated muscle tissue relies on a dynamic network of protein-protein interactions. Desmin intermediate filaments play a crucial role in heart and skeletal muscle by maintaining cell structure and contributing to various cellular processes. The assembly of desmin intermediate filaments requires aB-crystallin, which is involved in multiple cellular functions.
Article
Biochemistry & Molecular Biology
Dhritiman Dey, Adhiraj Dasgupta, Dipanjan Ghosh, Oindrila Bhattacharjee, Abhrajyoti Ghosh, Ayae Honda, Dhrubajyoti Chattopadhyay
Summary: This study successfully identified host factors that interact with Chandipura Virus (CHPV) and play a critical role in infection, including A2M, LRP1, and GRP78. Knocking out A2M has a severe effect on viral infection, indicating the potential role of these host proteins in viral entry into host cells.
Article
Biochemistry & Molecular Biology
Anna Dotsenko, Igor Sinelnikov, Aleksandra Rozhkova, Ivan Zorov, Arkady Sinitsyn
Summary: Xylanases play important roles in various industrial applications, such as feed additives, pulp and paper bleaching, and the production of bread, food, and drinks. Xylanases from the GH10 family show high thermostability and resistance to protein inhibitors, making them promising candidates for these processes. This study successfully improved the thermostability and enzymatic activity of GH10 xylanase, which could enhance its effectiveness as a feed additive and during pulp and paper bleaching.
Article
Biochemistry & Molecular Biology
M. Jaquet, M. Bengue, K. Lambert, G. Carnac, D. Misse, C. Bisbal
Summary: Changes to our environment have led to the emergence of human pathogens such as chikungunya virus, which is now a major public health concern. In this study, the interconnection between skeletal muscle cells metabolism, their differentiation stage, and the infectivity of chikungunya virus was investigated. The results showed that chikungunya virus infectivity is dependent on cell differentiation and metabolism and that the virus interferes with cellular metabolism.
