4.3 Article

Secretome analysis using a hollow fiber culture system for cancer biomarker discovery

Journal

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
Volume 1834, Issue 11, Pages 2285-2292

Publisher

ELSEVIER
DOI: 10.1016/j.bbapap.2013.01.033

Keywords

Hollow fiber culture (HFC) system; Secretome; Proteome; Cancer biomarker; Conditioned medium (CM)

Funding

  1. Ministry of Education, Taiwan, R.O.C. under the NCKU Project of Promoting Academic Excellence & Developing World Class Research Centers
  2. National Science Council [N5C99-2923-M-006-001-MY3, NSC100-2313-B-022-004, NSC100-2113-M-006-002-MY3, NSC101-2325-B-006-003]

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Secreted proteins, collectively referred to as the secretome, were suggested as valuable biomarkers in disease diagnosis and prognosis. However, some secreted proteins from cell cultures are difficult to detect because of their intrinsically low abundance; they are frequently masked by the released proteins from lysed cells and the substantial amounts of serum proteins used in culture medium. The hollow fiber culture (HFC) system is a commercially available system composed of small fibers sealed in a cartridge shell; cells grow on the outside of the fiber. Recently, because this system can help cells grow at a high density, it has been developed and applied in a novel analytical platform for cell secretome collection in cancer biomarker discovery. This article focuses on the advantages of the HFC system, including the effectiveness of the system for collection of secretomes, and reviews the process of cell secretome collection by the HFC system and proteomic approaches to discover cancer biomarkers. The HFC system not only provides a high-density three-dimensional (3D) cell culture system to mimic tumor growth conditions in vivo but can also accommodate numerous cells in a small volume, allowing secreted proteins to be accumulated and concentrated. In addition, cell lysis rates can be greatly reduced, decreasing the amount of contamination by abundant cytosolic proteins from lysed cells. Therefore, the HFC system is useful for preparing a wide range of proteins from cell secretomes and provides an effective method for collecting higher amounts of secreted proteins from cancer cells. This article is part of a Special Issue entitled: An Updated Secretome. (C) 2013 Elsevier B.V. All rights reserved.

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