Article
Biochemistry & Molecular Biology
Marloes van den Berg, Eva L. Peters, Robbert J. van der Pijl, Shengyi Shen, Leo M. A. Heunks, Henk L. Granzier, Coen A. C. Ottenheijm
Summary: Diaphragm weakness frequently occurs in mechanically ventilated critically ill patients, and this study suggests that modulating titin stiffness may affect its development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Vincent Loreau, Renate Rees, Eunice HoYee Chan, Waltraud Taxer, Kathrin Gregor, Bianka Mussil, Christophe Pitaval, Nuno Miguel Luis, Pierre Mangeol, Frank Schnorrer, Dirk Goerlich
Summary: Measuring the positions and dynamics of proteins in intact tissues or whole animals is challenging due to limited accessibility of large antibodies and potential interference of fluorescent proteins. In this study, a toolbox of nanobodies targeting different domains of key sarcomeric proteins was introduced, showing superior labeling qualities compared to antibodies. The toolbox was used to study sarcomere morphogenesis and revealed the presence of a gigantic isoform of Sallimus and the polar orientation of Projectin. The nanobodies were also shown to have high affinity-binding without affecting protein function.
Review
Biochemistry & Molecular Biology
Ashley A. Martin, Brian R. Thompson, Dongwoo Hahn, Addeli Bez Batti Angulski, Nora Hosny, Houda Cohen, Joseph M. Metzger
Summary: This review emphasizes the signaling components and regulatory mechanisms that impact cardiac sarcomere function, with a focus on the roles of the thick and thin filaments. The emerging field of inter-myofilament signaling and its important mediators are also discussed. Additionally, recent methods for studying the sarcomere under intact, physiologically relevant conditions are reviewed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cardiac & Cardiovascular Systems
Brandon Y. H. Chan, Andrej Roczkowsky, Woo Jung Cho, Mathieu Poirier, Consolato Sergi, Vic Keschrumrus, Jared M. Churko, Henk Granzier, Richard Schulz
Summary: The study found that MMP inhibitors can protect against DXR cardiotoxicity by reducing cardiac dysfunction and left ventricular remodelling, preventing myocardial fibrosis. This suggests that MMP inhibitors may be a potential prophylactic strategy to prevent heart injury during chemotherapy.
CARDIOVASCULAR RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Yoshihiko Miyata, Eisuke Nishida
Summary: The DYRK family comprises five related protein kinases, with DYRK1A linked to disorders such as Down syndrome. It was found that Hsp90 and Cdc37 are specific cellular protein chaperones for DYRK1B and DYRK4, playing a crucial role in quality control of these kinases.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2021)
Article
Biology
Florian Schueder, Pierre Mangeol, Eunice HoYee Chan, Renate Rees, Juergen Schuenemann, Ralf Jungmann, Dirk Goerlich, Frank Schnorrer
Summary: Sarcomeres, the force-producing units of striated muscles, depend on the titin protein for their nanoarchitecture. This study used nanobodies and super-resolution microscopy to determine the location of two Drosophila titin homologs in flight muscles. The findings reveal the presence of these titin homologs and their role in muscle structure, providing insights into the organization of sarcomeres in non-vertebrates.
Article
Biophysics
Atsuki Fukutani, Walter Herzog
Summary: The study aimed to examine the magnitude of the stretch-shortening cycle (SSC) effect in inhibited skeletal muscle force states. Results indicated that the SSC effect was preserved in the reduced force state and was significantly greater compared to the normal force state.
JOURNAL OF BIOMECHANICS
(2021)
Article
Developmental Biology
Nuno Miguel Luis, Frank Schnorrer
Summary: Muscles generate forces through sarcomeres to drive animal locomotion. During muscle development, sarcomeres assemble into long periodic myofibrils that mechanically connect to skeleton elements. Tension coordinates assembly of key sarcomeric components and transcriptional feedback mechanisms help coordinate myofibril assembly and maturation.
CELLS & DEVELOPMENT
(2021)
Article
Biochemistry & Molecular Biology
Lilian Tsai-Wei Lin, Abdul Razzaq, Sonja E. Di Gregorio, Soojie Hong, Brendan Charles, Marilene H. Lopes, Flavio Beraldo, Vania F. Prado, Marco A. M. Prado, Martin L. Duennwald
Summary: Protein misfolding is a key characteristic of most neurodegenerative diseases. Molecular chaperones such as Hsp90 and its co-chaperone Sti1 play a role in modulating the toxicity associated with TDP-43 misfolding and inclusion formation. Impaired Hsp90 function sensitizes cells to TDP-43 toxicity, while Sti1 specifically interacts with TDP-43 and strongly modulates its toxicity in a dose-dependent manner.
Review
Physiology
Andre Tomalka
Summary: Eccentric muscle loading has unique features including increased force, work, and performance while consuming less oxygen, reduced metabolic cost, and decreased muscle activity. This review summarizes approaches to explain muscular contraction dynamics and molecular and cellular mechanisms underlying eccentric muscle loading. It emphasizes the functional link between sarcomeric components, particularly the essential role of titin in skeletal muscle. The interaction between titin and actin can explain linear force increases in active lengthening experiments. This review aims to contribute to a detailed understanding of muscle behavior, force generation, and disease.
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Adeleye O. Adewale, Young-Hoon Ahn
Summary: Titin, a giant protein in the sarcomere, not only plays a crucial role in muscle contraction but also has versatile functions in sarcomere organization, passive force, mechanosensing, and signaling. Among its domains, N2A contributes to titin's functions in compliance, contraction, structural stability, and signaling by interacting with various protein partners such as actin filament, chaperones, stress-sensing proteins, and proteases, showcasing titin's complexity and importance in muscle function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
Florian Funk, Annette Kronenbitter, Malgorzata Isic, Vera Flocke, Simone Gorressen, Dominik Semmler, Maximilian Brinkmann, Katharina Beck, Oliver Steinhoff, Tanu Srivastava, David Monteiro Barbosa, Katharina Voigt, Luzhou Wang, Katharina Bottermann, Sebastian Koetter, Maria Grandoch, Ulrich Floegel, Martina Krueger, Joachim P. Schmitt
Summary: Type 2 diabetes mellitus is associated with adverse clinical outcome after myocardial infarction. This study found that in diabetic mice, the calcium cycling of cardiomyocytes was impaired, but contractile function was preserved due to increased myofilament calcium sensitivity and higher responsiveness to beta-adrenergic stimulation. However, ischemia-reperfusion further impaired cardiomyocyte function and calcium handling in diabetic mice, as these compensatory mechanisms failed.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Subhabrata Chaudhury, Penchala Narasimharao Meka, Monimoy Banerjee, Caitlin N. Kent, Brian S. J. Blagg
Summary: Hsp90 is responsible for folding and trafficking proteins associated with cancer progression. Existing inhibitors have undesired effects, leading to the development of isoform-selective inhibitors. Research shows that Hsp90 beta-selective inhibitors can overcome the detriments of pan-inhibition of Hsp90.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Pranjal Biswas, Yue Dai, Dennis J. Stuehr
Summary: IDO1 and TDO are crucial enzymes that convert tryptophan to L-kynurenine, impacting immune response and human biology. This study reveals the involvement of GAPDH and Hsp90 in the delivery and insertion of heme into IDO1 and TDO, with Hsp90 specifically driving heme insertion in IDO1.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Review
Cell Biology
Cheng Chang, Xin Tang, David T. Woodley, Mei Chen, Wei Li
Summary: For a long time, the cytosolic Hsp90 alpha and Hsp90 beta proteins have been considered evolutionarily conserved, ATP-driven chaperones that play a crucial role in basic research and clinical trials. Recent studies have challenged this notion and highlighted the failures of paradigm-based clinical trials. It is now known that Hsp90 alpha and Hsp90 beta are ubiquitously expressed in all cell types but have distinct and irreplaceable functions. Understanding the differences between Hsp90 alpha and Hsp90 beta could lead to new therapeutic approaches for human disorders such as cancer.
Article
Cardiac & Cardiovascular Systems
Christoph Maack, Thomas Eschenhagen, Nazha Hamdani, Frank R. Heinzel, Alexander R. Lyon, Dietmar J. Manstein, Joseph Metzger, Zoltan Papp, Carlo G. Tocchetti, M. Birhan Yilmaz, Stefan D. Anker, Jean-Luc Balligand, Johann Bauersachs, Dirk Brutsaert, Lucie Carrier, Stefan Chlopicki, John G. Cleland, Rudolf A. de Boer, Alexander Dietl, Rodolphe Fischmeister, Veli-Pekka Harjola, Stephane Heymans, Denise Hilfiker-Kleiner, Johannes Holzmeister, Gilles de Keulenaer, Giuseppe Limongelli, Wolfgang A. Linke, Lars H. Lund, Josep Masip, Marco Metra, Christian Mueller, Burkert Pieske, Piotr Ponikowski, Arsen Ristic, Frank Ruschitzka, Petar M. Seferovic, Hadi Skouri, Wolfram H. Zimmermann, Alexandre Mebazaa
EUROPEAN HEART JOURNAL
(2019)
Article
Clinical Neurology
L. Winter, A. Unger, C. Berwanger, M. Spoerrer, M. Tuerk, F. Chevessier, K. -H. Strucksberg, U. Schloetzer-Schrehardt, I. Wittig, W. H. Goldmann, K. Marcus, W. A. Linke, C. S. Clemen, R. Schroeder
NEUROPATHOLOGY AND APPLIED NEUROBIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Markus Horsthemke, Lauryl M. J. Nutter, Anne C. Bachg, Boris V. Skryabin, Ulrike Honnert, Thomas Zobel, Sven Bogdan, Monika Stoll, Matthias D. Seidl, Frank U. Mueller, Ursula Ravens, Andreas Unger, Wolfgang A. Linke, Pim R. R. van Gorp, Antoine A. F. de Vries, Martin Baehler, Peter J. Hanley
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Hematology
Carolin Christina Drost, Alexandros Rovas, Kristina Kusche-Vihrog, Paul Van Slyke, Harold Kim, Van C. Hoang, Jason T. Maynes, Dirk Oliver Wennmann, Hermann Pavenstaedt, Wolfgang Linke, Alexander Lukasz, Bettina Hesse, Philipp Kuempers
THROMBOSIS AND HAEMOSTASIS
(2019)
Article
Urology & Nephrology
Bettina Hesse, Alexandros Rovas, Konrad Buscher, Kristina Kusche-Vihrog, Marcus Brand, Giovana Seno Di Marco, Jan T. Kielstein, Hermann Pavenstaedt, Wolfgang A. Linke, Jerzy-Roch Nofer, Philipp Kuempers, Alexander Lukasz
KIDNEY INTERNATIONAL
(2020)
Article
Multidisciplinary Sciences
Yuanyuan Dai, Asset Amenov, Nadezda Ignatyeva, Andreas Koschinski, Hang Xu, Poh Loong Soong, Malte Tiburcy, Wolfgang A. Linke, Manuela Zaccolo, Gerd Hasenfuss, Wolfram-Hubertus Zimmermann, Antje Ebert
SCIENTIFIC REPORTS
(2020)
Article
Multidisciplinary Sciences
Jaime Andres Rivas-Pardo, Yong Li, Zsolt Martonfalvi, Rafael Tapia-Rojo, Andreas Unger, Angel Fernandez-Trasancos, Elias Herrero-Galan, Diana Velazquez-Carreras, Julio M. Fernandez, Wolfgang A. Linke, Jorge Alegre-Cebollada
NATURE COMMUNICATIONS
(2020)
Article
Physiology
Melissa Herwig, Detmar Kolijn, Maria Lodi, Soraya Hoelper, Arpad Kovacs, Zoltan Papp, Kornelia Jaquet, Peter Haldenwang, Cris Dos Remedios, Peter H. Reusch, Andreas Muegge, Marcus Krueger, Jens Fielitz, Wolfgang A. Linke, Nazha Hamdani
FRONTIERS IN PHYSIOLOGY
(2020)
Article
Physiology
Detmar Kolijn, Arpad Kovacs, Melissa Herwig, Maria Lodi, Marcel Sieme, Abdulatif Alhaj, Peter Sandner, Zoltan Papp, Peter H. Reusch, Peter Haldenwang, Ines Falcao-Pires, Wolfgang A. Linke, Kornelia Jaquet, Sophie Van Linthout, Andreas Muegge, Carsten Tschoepe, Nazha Hamdani
FRONTIERS IN PHYSIOLOGY
(2020)
Article
Multidisciplinary Sciences
Sandra Swist, Andreas Unger, Yong Li, Anja Voge, Marion von Frieling-Salewsky, Asa Skarlen, Nicola Cacciani, Thomas Braun, Lars Larsson, Wolfgang A. Linke
NATURE COMMUNICATIONS
(2020)
Article
Biology
Yong Li, Anthony L. Hessel, Andreas Unger, David Ing, Jannik Recker, Franziska Koser, Johanna K. Freundt, Wolfgang A. Linke
Editorial Material
Physiology
Anthony L. Hessel, Wolfgang A. Linke
JOURNAL OF GENERAL PHYSIOLOGY
(2021)
Review
Cardiac & Cardiovascular Systems
Christine M. Loescher, Anastasia J. Hobbach, Wolfgang A. Linke
Summary: This review provides an overview of the changes in cardiac titin properties at a molecular level, including the role isoform diversity and post-translational modifications play in regulating myocardial function, and discusses the importance of this regulation imbalance in heart disease.
CARDIOVASCULAR RESEARCH
(2022)
Article
Multidisciplinary Sciences
Anthony L. Hessel, Weikang Ma, Nicole Mazara, Paige E. Rice, Devin Nissen, Henry Gong, Michel Kuehn, Thomas Irving, Wolfgang A. Linke
Summary: This study found that skeletal muscle force production increases with longer muscle lengths due to length-dependent priming of thick filament proteins in the muscle unit before contraction. The research also discovered that bridge proteins between thick and thin filaments, potentially myosin-binding protein C, play a role in the length-dependent priming of thin filaments. These bridges can be forcibly ruptured via high-speed stretches.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Matthias Rohrbeck, Verena Hoerr, Ilaria Piccini, Boris Greber, Jan Sebastian Schulte, Sara-Sophie Huebner, Elena Jeworutzki, Carsten Theiss, Veronika Matschke, Joerg Stypmann, Andreas Unger, Huyen Tran Ho, Paul Disse, Nathalie Strutz-Seebohm, Cornelius Faber, Frank Ulrich Mueller, Stephan Ludwig, Ursula Rescher, Wolfgang A. A. Linke, Karin Klingel, Karin Busch, Stefan Peischard, Guiscard Seebohm
Summary: Using a transgenic mouse strain (TG) containing a CVB3 Delta VP0 genome, we have revealed virus-mediated cardiac pathophysiological processes in vivo and in vitro. Transgenic mice showed significant alterations in cardiac function, pathologic ECG alterations, calcium homeostasis, intracellular organization, and gene expression. The results also suggest that mitochondrial impairment may contribute to cardiac contractile dysfunction.
Article
Biochemistry & Molecular Biology
G. F. Senguel, R. Mishra, E. Candiello, P. Schu
Summary: AP2 forms AP2 CCV with clathrin and other coat proteins, and synapses contain different types of CCV. The stability and composition of CCV are regulated by various factors, including Hsc70 and phosphorylation patterns. The knockout of the AP1/O1B complex disrupts synaptic vesicle recycling and endosomal protein sorting, leading to upregulation of endocytosis. Stable CCV, termed stCCV, have distinct characteristics and specialized functions in synaptic plasticity. The phosphorylation of Hsc70 and the levels of kinases play a crucial role in regulating the stability and disassembly of clathrin in CCV.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Martin Fluck, Colline Sanchez, Vincent Jacquemond, Christine Berthier, Marie-Noelle Giraud, Daniel Jacko, Kathe Bersiner, Sebastian Gehlert, Guus Baan, Richard T. Jaspers
Summary: Enhancing CaMKII signaling improves fatigue resistance and contractile characteristics of skeletal muscle by enhancing calcium release.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Letter
Biochemistry & Molecular Biology
Federica Coppola, Sara Monaci, Alessandro Falsini, Carlo Aldinucci, Irene Filippi, Daniela Rossi, Fabio Carraro, Antonella Naldini
Summary: The adaptor protein p62 plays a crucial role in maintaining the survival of dendritic cells (DCs) under hypoxic conditions by preserving Erk1/2 phosphorylation and reducing AMPK activation, thus extending their lifespan to ensure their functions in hypoxic microenvironments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Jenifer Pendiuk Goncalves, Jorvani Cruz Villarreal, Sierra A. Walker, Xuan Ning Sharon Tan, Chad Borges, Joy Wolfram
Summary: This study used a mass spectrometry-based approach to assess the differences in glycan features between extracellular vesicles (EVs) and originating cells. The results showed that EVs selectively enriched specific glycan features, particularly those associated with binding to the extracellular matrix. The study also found differences in EV glycan sorting between different metastatic cell lines and mouse models, indicating a potential role of glycan diversity in the metastatic process.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
De-ao Gong, Peng Zhou, Wen-yi Chang, Jia-yao Yang, Yan-lai Zhang, Ai-long Huang, Ni Tang, Kai Wang
Summary: Liver cancer, ranked sixth globally, is a major contributor to cancer-related mortality. Metastasis is the main cause of treatment failure and deaths in liver cancer. The SPOP-CREB5-MET axis plays a significant role in liver cancer metastasis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ning Huang, Jun Tang, Xiaoyao Yi, Maoxin Zhang, Bin Li, Yuan Cheng, Jin Chen
Summary: This study reveals that glioma-derived S100A9 can induce microglial M2 polarization, inhibit CD8+ T lymphocytes, and promote immunosuppression. The mechanism is related to the interaction with alpha v133 integrin and subsequent activation of AKT1 in microglia. The expression of S100A9 is positively associated with CD206 expression and negatively correlated with CD8+ T lymphocyte accumulation in the TME, suggesting a potential role of S100A9 in regulating the tumor microenvironment and immune evasion in glioma.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Yomna S. Abd El-Aziz, Matthew J. McKay, Mark P. Molloy, Betty McDowell, Elizabeth Moon, Loretta Sioson, Amy Sheen, Angela Chou, Anthony J. Gill, Patric J. Jansson, Sumit Sahni
Summary: This study identified a novel combination of autophagy inhibitors that can effectively inhibit the proliferation of oral squamous cell carcinoma (OSCC) cells, including both chemosensitive and chemoresistant cells. This research is important for the development of new therapies for advanced OSCC tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Luojia Liu, Xiaoqiang Liu, Ying Chen, Meng Kong, Jinghong Zhang, Min Jiang, Hongling Zhou, Jinrui Yang, Xu Chen, Ze Zhang, Chao Wu, Xupin Jiang, Jiaping Zhang
Summary: Our study revealed that the Paxillin/HDAC6 signaling pathway regulates microtubule acetylation in electric field-guided keratinocyte migration.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Julia Weikum, Jeroen F. van Dyck, Saranya Subramani, David P. Klebl, Merete Storflor, Stephen P. Muench, Soren Abel, Frank Sobott, J. Preben Morth
Summary: The study reveals the complex interaction between bacterial magnesium transporter A (MgtA) and cardiolipin 18:1 and cardiolipin 16:0, highlighting the importance of lipid environment in protein activity and stability. Further understanding of Mg2+ homeostasis in bacteria will provide insights into bacterial infections.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Sumit Kinger, Yuvraj Anandrao Jagtap, Ankur Rakesh Dubey, Prashant Kumar, Akash Choudhary, Rohan Dhiman, Vijay Kumar Prajapati, Deepak Chitkara, Krishna Mohan Poluri, Amit Mishra
Summary: Efficient protein synthesis and quality control mechanisms are crucial for maintaining proteostasis and preventing neurodegeneration. This study demonstrates that treating cells with Lanosterol can enhance the proteolytic activity of Proteasome and promote the removal of misfolded proteins, suggesting a potential therapeutic approach for abnormal protein accumulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Karolina Stepien, Adrianna Skoneczna, Monika Kula-Maximenko, Lukasz Jurczyk, Mateusz Molon
Summary: The replication of DNA requires a complex machinery called the replisome, which is highly conserved across species. One crucial component of the replisome is the CMG helicase complex, which unwinds DNA and coordinates the assembly and function of other replisome components. In this study, the impact of the absence of one copy of the CMG complex genes on the physiology and aging of yeast cells was investigated. The findings showed disruptions in the cell cycle, extended doubling times, and alterations in the biochemical profile of these cells. Importantly, it was found that heterozygous cells for CMG helicase genes exhibited increased reproductive potential and delayed aging. The study also highlighted potential therapeutic targets for cancer treatment using yeast.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Nishadh Rathod, Guadalupe Guerrero-Serna, Howard S. Young, L. Michel Espinoza-Fonseca
Summary: This study reveals that replacing Lys27 with Asn enhances the inhibitory potency of MLN without affecting SERCA's affinity for Ca2+. The findings suggest that the SERCA site modulating Ca2+ affinity also functions as a catalytic activity switch.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Can Jiang, Chunyang Zhang, Min Dai, Fuyan Wang, Sa Xu, Dan Han, Yanyan Wang, Yajie Cao, Yanyan Liang, Ziyu Zhang, Lina Yan, Yujun Shen, Kewu He, Yuxian Shen, Jun Liu
Summary: The phosphorylation of p65 and the expression of SUMO1 are increased in cancer tissues of HCC patients, and there is a positive correlation between SUMO1 and phosphorylated p65. SUMOylation of p65 by SUMO1 promotes p65 nuclear import and enhances NF-xB activity. Both SUMOylation and phosphorylation of p65 increase the viability and invasion of hepatoma cells, and decrease cell apoptosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ming-Fo Hsu, Yoshihiro Ito, Jai Prakash Singh, Shu-Fang Hsu, Alan Wells, Kuang-Yu Jen, Tzu-Ching Meng, Fawaz G. Haj
Summary: This study identified alpha-actinin4 as a novel substrate of PTP1B in podocytes and demonstrated their interaction in regulating podocyte function. Targeting PTP1B and alpha-actinin4 could be a potential therapeutic approach for podocyte injury.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Paulo F. V. Bizerra, Eduardo H. Gilglioni, Hang Lam Li, Simei Go, Ronald P. J. Oude Elferink, Arthur J. Verhoeven, Jung -Chin Chang
Summary: This study investigates the role of cyclic AMP (cAMP) in glycogen metabolism and reveals that cAMP regulates glycogenolysis in opposite directions depending on its site of synthesis within cells and downstream effectors. The canonical tmAC-cAMP-PKA signaling promotes glycogenolysis, while the non-canonical sAC-cAMP-Epac1 signaling suppresses glycogenolysis. This highlights the importance of cAMP microdomain organization for distinct metabolic regulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)