Review
Genetics & Heredity
Guangzhao Guan, Richard D. D. Cannon, Dawn E. E. Coates, Li Mei
Summary: The mechanical properties of cells play an important role in tissue homeostasis and various cellular processes. These properties are determined by the cytoskeleton, which is a complex and dynamic network of microfilaments, intermediate filaments, and microtubules. The Rho-kinase/ROCK signaling pathway regulates the architecture of this cytoskeletal network, influencing cell behavior.
Review
Biochemistry & Molecular Biology
Thiery De Serres-Berard, Siham Ait Benichou, Dominic Jauvin, Mohamed Boutjdir, Jack Puymirat, Mohamed Chahine
Summary: DM1 is a dominant genetic disease that can be treated by altering the chemical design of antisense oligonucleotides. Various strategies involving chemical modifications and conjugation with specific functional molecules have been shown to improve the potency in muscle and cardiac tissues. In addition, intrathecal administration can be used to treat central nervous system defects in DM1.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Xiaopeng Shen, Zhongxian Liu, Chunguang Wang, Feng Xu, Jingyi Zhang, Meng Li, Yang Lei, Ao Wang, Chao Bi, Guoping Zhu
Summary: The research discovered that Postn gene was significantly upregulated in DM1 myogenesis, and knocking down Postn could successfully rescue myogenesis defects, suggesting that Postn could be a potential therapeutic target for treating myogenesis defects in DM1.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Kathryn N. Becker, Krista M. Pettee, Amanda Sugrue, Kevin A. Reinard, Jason L. Schroeder, Kathryn M. Eisenmann
Summary: Targeting mDia is an effective strategy against invasion and tumor microtube networking, while inhibiting ROCK is contraindicated for treating brain cancer.
Review
Neurosciences
De Serres-Berard Thiery, Marion Pierre, Mohamed Chahine, Jack Puymirat
Summary: Myotonic dystrophy type 1 is a multisystemic and heterogeneous disorder caused by gene mutations. There is a congenital form with brain structure abnormalities and an adult-onset form with neurodegenerative features and cognitive deficits. Promising therapies targeting molecular mechanisms are in development for adult-onset DM1, but their relevance for treating cognitive impairment in CDM1 remains to be elucidated.
NEUROBIOLOGY OF DISEASE
(2021)
Review
Biochemistry & Molecular Biology
Janel A. M. Peterson, Thomas A. A. Cooper
Summary: Myotonic dystrophy (DM) is a highly variable multisystemic disorder that affects one in 8000 individuals. Although most research has focused on the symptoms and pathological mechanisms in muscle and brain, surveys have found a high prevalence of gastrointestinal (GI) symptoms in DM patients. Clinical studies have identified chronic and progressive dysfunction in various parts of the GI system. The pathological mechanisms leading to this dysfunction are not well understood, but it is suggested that misregulation of alternative splicing in smooth muscle may contribute to GI dysfunction in DM.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Vanessa Todorow, Stefan Hintze, Alastair R. W. Kerr, Andreas Hehr, Benedikt Schoser, Peter Meinke
Summary: Myotonic dystrophy type 1 (DM1) is a disease caused by CTG-repeat expansions that primarily affects muscles and brain. In addition to known information on alternative splicing of genes involved in the pathology, there may be other factors contributing to the disease development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Jessica Stoodley, Francisco Vallejo-Bedia, David Seone-Miraz, Manuel Debasa-Mouce, Matthew J. A. Wood, Miguel A. Varela
Summary: DM1 is a common muscular dystrophy that can potentially be treated with antisense therapy. Delivery of antisense molecules to target tissues remains a challenge, but conjugation to lipids, peptides, or antibodies has shown promise in improving therapeutic efficacy and tissue penetration. Clinical trials of antisense therapies are currently underway, raising hopes for an approved treatment for DM1 patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Ashwin K. Chetty, Byung Hak Ha, Titus J. Boggon
Summary: This review focuses on the signaling pathways mediated by Rho family small GTPases and the specific roles of type II PAKs. The selectivity of Rho GTPases for PAK family members and the molecular basis for substrate recognition and regulation of signaling are discussed. The cross talk between Rho family small GTPases and the involvement of PAKs in diseases are also addressed.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Multidisciplinary Sciences
Mengwei Li, Limei Zhang, Xinyan Liu, Guoqiang Wang, Jian Lu, Jifeng Guo, Hongjie Wang, Jinpeng Xu, Yi Zhang, Na Li, You Zhou
Summary: This study found that low-intensity exercise training can improve the heart function and structure of spontaneously hypertensive rats by inhibiting the RhoA/ROCK signaling pathway, providing further evidence for this protective effect.
SCIENTIFIC REPORTS
(2022)
Article
Cell Biology
Claudia Brito, Joana M. Pereira, Francisco S. Mesquita, Didier Cabanes, Sandra Sousa
Summary: Non-muscle myosin 2A (NM2A) is a cytoskeletal enzyme involved in cell adhesion and motility. Its phosphorylation at residue 158 regulates cytoskeletal organization, focal adhesion assembly/disassembly, and cell migration. This phosphorylation is promoted by Listeria monocytogenes infection and is dependent on Src kinase. We propose NM2A pTyr(158) as a novel regulatory mechanism for actomyosin cytoskeleton organization.
Article
Chemistry, Medicinal
Upul K. Bandarage, John Court, Huai Gao, Suganthini Nanthakumar, Jon H. Come, Simon Giroux, Jeremy Green
Summary: The study extended the structure-activity relationship exploration of the 7-azaindole series to identify new compounds with excellent ROCK potency, PKA selectivity, and stability in microsomes and hepatocytes for further evaluation.
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Emma R. Hinkle, Hannah J. Wiedner, Eduardo Torres, Micaela Jackson, Adam J. Black, R. Eric Blue, Sarah E. Harris, Bryan B. Guzman, Gabrielle M. Gentile, Eunice Y. Lee, Yi-Hsuan Tsai, Joel Parker, Daniel Dominguez, Jimena Giudice
Summary: This study found that alternative splicing programs play an important role in organ development and diseases. Extensive splicing changes occur in genes related to vesicle-mediated trafficking during postnatal mouse heart development. PTBP1 and quaking were identified as regulators of splicing in muscle cells.
Review
Biochemistry & Molecular Biology
Mani S. Mahadevan, Ramesh S. Yadava, Mahua Mandal
Summary: DM1 is a multi-systemic disorder affecting various organs, with the heart being one of the most severely impacted. Cardiac conduction defects and arrhythmias are common in adults with DM1, leading to potential sudden death. Understanding of cardiac pathology in DM1 is still limited, with cardiac magnetic resonance imaging (CMR) emerging as a valuable biomarker.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Ram Kumar, Yogesh Chander, Nitin Khandelwal, Assim Verma, Krishan Dutt Rawat, Brij N. Shringi, Yash Pal, Bhupendra N. Tripathi, Sanjay Barua, Naveen Kumar
Summary: The ROCK1/MLC2 signaling pathway promotes buffalo pox virus replication by preventing viral mRNA decay. Inhibitors targeting this pathway may have therapeutic effects against buffalopox.
SCIENTIFIC REPORTS
(2022)
Review
Nutrition & Dietetics
Marina C. Oliveira, Julia Vullings, Fons A. J. van de Loo
Article
Endocrinology & Metabolism
G. Ascone, I. Di Ceglie, B. Walgreen, A. W. Sloetjes, E. Lindhout, I. Bot, F. A. J. van de Loo, M. I. Koenders, P. M. van der Kraan, A. B. Blom, M. H. J. van den Bosch, P. L. E. M. van Lent
Article
Multidisciplinary Sciences
Onno J. Arntz, Bartijn C. H. Pieters, Peter L. E. M. van Lent, Marije I. Koenders, Peter M. van der Kraan, Fons A. J. van de Loo
Article
Biotechnology & Applied Microbiology
Marina C. Oliveira, Bartijn C. H. Pieters, Polianna B. Guimaraes, Leticia F. Duffles, Joyce E. Heredia, Ana L. M. Silveira, Amanda C. C. Oliveira, Mauro M. Teixeira, Adaliene V. M. Ferreira, Tarcilia A. Silva, Fons A. J. van de Loo, Soraia Macari
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2020)
Article
Multidisciplinary Sciences
Van Thai Ha, Dusko Lainscek, Bernd Gesslbauer, Eva Jarc-Jovicic, Tuulia Hyotylainen, Nejc Ilc, Katja Lakota, Matija Tomsic, Fons A. J. van de Loo, Valery Bochkov, Toni Petan, Roman Jerala, Mateja Mancek-Keber
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Immunology
Julia Vullings, Juliana P. Vago, Claire E. J. Waterborg, Rogier M. Thurlings, Marije I. Koenders, Peter L. E. M. van Lent, Peter M. van der Kraan, Flavio A. Amaral, Fons A. J. van de Loo
JOURNAL OF IMMUNOLOGY RESEARCH
(2020)
Review
Biochemistry & Molecular Biology
Koen van den Dries, Jack Fransen, Alessandra Cambi
Summary: Correlative light and electron microscopy (CLEM) is a powerful imaging approach that combines light and electron microscopy, and has wide applications in biology. This review summarizes the important developments in CLEM for biological research, with a focus on the combination of fluorescence microscopy and electron microscopy.
Editorial Material
Biochemistry & Molecular Biology
Jan Willem Borst, Jack Fransen, Stefanie Weidtkamp-Peters, Yvonne Stahl
Article
Neurosciences
Malou Zuidscherwoude, Mark K. K. van Goor, Sara R. R. Roig, Niky Thijssen, Merijn van Erp, Jack Fransen, Jenny van der Wijst, Joost G. G. Hoenderop
Summary: This study characterizes the interaction between functional TRPV5 and CaM, demonstrating that they interact in the absence of Ca2+ and the interaction is intensified with increasing Ca2+ concentrations. The CaM C-lobe achieves channel pore blocking, leading to channel inactivation. Furthermore, the study shows a Ca2+-dependent binding stoichiometry at the single channel level. This research proposes a new CaM-dependent regulation model for TRPV5, involving apoCaM interaction and lobe-specific actions, which is of significant physiological relevance given its role in Ca2+ transport in the kidney.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Article
Rheumatology
Arjen B. Blom, Martijn H. van den Bosch, Esmeralda N. Blaney Davidson, Johannes Roth, Thomas Vogl, Fons A. van de Loo, Marije Koenders, Peter M. van der Kraan, Edwin J. Geven, Peter L. van Lent
ARTHRITIS RESEARCH & THERAPY
(2020)
Proceedings Paper
Optics
Kalloor Joseph Francis, Marvin Xavierselvan, Mithun Kuniyil Ajith Singh, Srivalleesha Mallidi, Conny van der Laken, Fons van de Loo, Wiendelt Steenbergen
PHOTONS PLUS ULTRASOUND: IMAGING AND SENSING 2020
(2020)
Meeting Abstract
Rheumatology
J. Aarts, A. Van Caam, M. Helsen, B. Walgreen, E. Vitters, F. Van de Loo, P. Van Lent, P. Van der Kraan, M. Koenders
ANNALS OF THE RHEUMATIC DISEASES
(2020)
Meeting Abstract
Orthopedics
R. Wiegertjes, A. van Caam, P. van der Kraan, F. van de Loo, E. Blaney Davidson
OSTEOARTHRITIS AND CARTILAGE
(2020)
Meeting Abstract
Orthopedics
N. Kruisbergen, Y. van Gemert, A. Blom, F. van de Loo, P. van der Kraan, M. van den Bosch, P. van Lent
OSTEOARTHRITIS AND CARTILAGE
(2020)
Meeting Abstract
Orthopedics
I. Di Ceglie, M. Broeren, C. Waterborg, D. Dorst, R. Thurlings, P. Laverman, F. van de Loo, P. van der Kraan, M. Koenders, P. van Lent
OSTEOARTHRITIS AND CARTILAGE
(2020)
Article
Biochemistry & Molecular Biology
G. F. Senguel, R. Mishra, E. Candiello, P. Schu
Summary: AP2 forms AP2 CCV with clathrin and other coat proteins, and synapses contain different types of CCV. The stability and composition of CCV are regulated by various factors, including Hsc70 and phosphorylation patterns. The knockout of the AP1/O1B complex disrupts synaptic vesicle recycling and endosomal protein sorting, leading to upregulation of endocytosis. Stable CCV, termed stCCV, have distinct characteristics and specialized functions in synaptic plasticity. The phosphorylation of Hsc70 and the levels of kinases play a crucial role in regulating the stability and disassembly of clathrin in CCV.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Martin Fluck, Colline Sanchez, Vincent Jacquemond, Christine Berthier, Marie-Noelle Giraud, Daniel Jacko, Kathe Bersiner, Sebastian Gehlert, Guus Baan, Richard T. Jaspers
Summary: Enhancing CaMKII signaling improves fatigue resistance and contractile characteristics of skeletal muscle by enhancing calcium release.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Letter
Biochemistry & Molecular Biology
Federica Coppola, Sara Monaci, Alessandro Falsini, Carlo Aldinucci, Irene Filippi, Daniela Rossi, Fabio Carraro, Antonella Naldini
Summary: The adaptor protein p62 plays a crucial role in maintaining the survival of dendritic cells (DCs) under hypoxic conditions by preserving Erk1/2 phosphorylation and reducing AMPK activation, thus extending their lifespan to ensure their functions in hypoxic microenvironments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Jenifer Pendiuk Goncalves, Jorvani Cruz Villarreal, Sierra A. Walker, Xuan Ning Sharon Tan, Chad Borges, Joy Wolfram
Summary: This study used a mass spectrometry-based approach to assess the differences in glycan features between extracellular vesicles (EVs) and originating cells. The results showed that EVs selectively enriched specific glycan features, particularly those associated with binding to the extracellular matrix. The study also found differences in EV glycan sorting between different metastatic cell lines and mouse models, indicating a potential role of glycan diversity in the metastatic process.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
De-ao Gong, Peng Zhou, Wen-yi Chang, Jia-yao Yang, Yan-lai Zhang, Ai-long Huang, Ni Tang, Kai Wang
Summary: Liver cancer, ranked sixth globally, is a major contributor to cancer-related mortality. Metastasis is the main cause of treatment failure and deaths in liver cancer. The SPOP-CREB5-MET axis plays a significant role in liver cancer metastasis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ning Huang, Jun Tang, Xiaoyao Yi, Maoxin Zhang, Bin Li, Yuan Cheng, Jin Chen
Summary: This study reveals that glioma-derived S100A9 can induce microglial M2 polarization, inhibit CD8+ T lymphocytes, and promote immunosuppression. The mechanism is related to the interaction with alpha v133 integrin and subsequent activation of AKT1 in microglia. The expression of S100A9 is positively associated with CD206 expression and negatively correlated with CD8+ T lymphocyte accumulation in the TME, suggesting a potential role of S100A9 in regulating the tumor microenvironment and immune evasion in glioma.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Yomna S. Abd El-Aziz, Matthew J. McKay, Mark P. Molloy, Betty McDowell, Elizabeth Moon, Loretta Sioson, Amy Sheen, Angela Chou, Anthony J. Gill, Patric J. Jansson, Sumit Sahni
Summary: This study identified a novel combination of autophagy inhibitors that can effectively inhibit the proliferation of oral squamous cell carcinoma (OSCC) cells, including both chemosensitive and chemoresistant cells. This research is important for the development of new therapies for advanced OSCC tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Luojia Liu, Xiaoqiang Liu, Ying Chen, Meng Kong, Jinghong Zhang, Min Jiang, Hongling Zhou, Jinrui Yang, Xu Chen, Ze Zhang, Chao Wu, Xupin Jiang, Jiaping Zhang
Summary: Our study revealed that the Paxillin/HDAC6 signaling pathway regulates microtubule acetylation in electric field-guided keratinocyte migration.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Julia Weikum, Jeroen F. van Dyck, Saranya Subramani, David P. Klebl, Merete Storflor, Stephen P. Muench, Soren Abel, Frank Sobott, J. Preben Morth
Summary: The study reveals the complex interaction between bacterial magnesium transporter A (MgtA) and cardiolipin 18:1 and cardiolipin 16:0, highlighting the importance of lipid environment in protein activity and stability. Further understanding of Mg2+ homeostasis in bacteria will provide insights into bacterial infections.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Sumit Kinger, Yuvraj Anandrao Jagtap, Ankur Rakesh Dubey, Prashant Kumar, Akash Choudhary, Rohan Dhiman, Vijay Kumar Prajapati, Deepak Chitkara, Krishna Mohan Poluri, Amit Mishra
Summary: Efficient protein synthesis and quality control mechanisms are crucial for maintaining proteostasis and preventing neurodegeneration. This study demonstrates that treating cells with Lanosterol can enhance the proteolytic activity of Proteasome and promote the removal of misfolded proteins, suggesting a potential therapeutic approach for abnormal protein accumulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Karolina Stepien, Adrianna Skoneczna, Monika Kula-Maximenko, Lukasz Jurczyk, Mateusz Molon
Summary: The replication of DNA requires a complex machinery called the replisome, which is highly conserved across species. One crucial component of the replisome is the CMG helicase complex, which unwinds DNA and coordinates the assembly and function of other replisome components. In this study, the impact of the absence of one copy of the CMG complex genes on the physiology and aging of yeast cells was investigated. The findings showed disruptions in the cell cycle, extended doubling times, and alterations in the biochemical profile of these cells. Importantly, it was found that heterozygous cells for CMG helicase genes exhibited increased reproductive potential and delayed aging. The study also highlighted potential therapeutic targets for cancer treatment using yeast.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Nishadh Rathod, Guadalupe Guerrero-Serna, Howard S. Young, L. Michel Espinoza-Fonseca
Summary: This study reveals that replacing Lys27 with Asn enhances the inhibitory potency of MLN without affecting SERCA's affinity for Ca2+. The findings suggest that the SERCA site modulating Ca2+ affinity also functions as a catalytic activity switch.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Can Jiang, Chunyang Zhang, Min Dai, Fuyan Wang, Sa Xu, Dan Han, Yanyan Wang, Yajie Cao, Yanyan Liang, Ziyu Zhang, Lina Yan, Yujun Shen, Kewu He, Yuxian Shen, Jun Liu
Summary: The phosphorylation of p65 and the expression of SUMO1 are increased in cancer tissues of HCC patients, and there is a positive correlation between SUMO1 and phosphorylated p65. SUMOylation of p65 by SUMO1 promotes p65 nuclear import and enhances NF-xB activity. Both SUMOylation and phosphorylation of p65 increase the viability and invasion of hepatoma cells, and decrease cell apoptosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ming-Fo Hsu, Yoshihiro Ito, Jai Prakash Singh, Shu-Fang Hsu, Alan Wells, Kuang-Yu Jen, Tzu-Ching Meng, Fawaz G. Haj
Summary: This study identified alpha-actinin4 as a novel substrate of PTP1B in podocytes and demonstrated their interaction in regulating podocyte function. Targeting PTP1B and alpha-actinin4 could be a potential therapeutic approach for podocyte injury.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Paulo F. V. Bizerra, Eduardo H. Gilglioni, Hang Lam Li, Simei Go, Ronald P. J. Oude Elferink, Arthur J. Verhoeven, Jung -Chin Chang
Summary: This study investigates the role of cyclic AMP (cAMP) in glycogen metabolism and reveals that cAMP regulates glycogenolysis in opposite directions depending on its site of synthesis within cells and downstream effectors. The canonical tmAC-cAMP-PKA signaling promotes glycogenolysis, while the non-canonical sAC-cAMP-Epac1 signaling suppresses glycogenolysis. This highlights the importance of cAMP microdomain organization for distinct metabolic regulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
