Review
Biochemistry & Molecular Biology
Jason Tasoulas, Sonal Srivastava, Xiaonan Xu, Valentina Tarasova, Anastasios Maniakas, Florian A. Karreth, Antonio L. Amelio
Summary: The head and neck region is highly susceptible to cancer, with over 1.5 million new cases reported worldwide in 2020. Significant progress has been made in understanding the disease mechanisms and tailoring treatments to individual tumor characteristics using genetically engineered mouse models. These models have successfully replicated various aspects of head and neck cancers.
Article
Oncology
Yuki Ohkawa, Pu Zhang, Hiroyuki Momota, Akira Kato, Noboru Hashimoto, Yuhsuke Ohmi, Robiul H. Bhuiyan, Yesmin Farhana, Atsushi Natsume, Toshihiko Wakabayashi, Keiko Furukawa, Koichi Furukawa
Summary: This study revealed that deficiency of GD3 synthase attenuated the progression of gliomas in a mouse model, leading to prolonged lifespan and low-grade pathology in generated gliomas. It also showed that GD3S enhances glioma progression through the Ap2 alpha-MMP9 axis, with associated alterations in gene expression levels.
Review
Oncology
Kajal Biswas, Altaf Mohammed, Shyam K. Sharan, Robert H. Shoemaker
Summary: Advances in molecular diagnostics have improved the diagnosis and understanding of hereditary cancers. Preclinical experimental models, such as genetically engineered mouse (GEM) models, play a crucial role in studying and preventing cancer in high-risk populations.
Review
Oncology
Reihaneh Alsadat Mahmoudian, Moein Farshchian, Mohammad Reza Abbaszadegan
Summary: Esophageal cancer is a leading cause of cancer-related deaths worldwide, and understanding the mechanisms of its development is crucial for improving patient outcomes. Genetically engineered mouse models provide valuable insights into cancer pathogenesis and treatment strategies, despite the differences between mice and humans. By addressing challenges in modeling and utilizing advanced technologies, researchers can maximize the value of studying esophageal cancer in GEMMs.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Chemistry, Physical
Jianhai Chen, Jianwei Tan, Jian Li, Wenxiang Cheng, Liqing Ke, Anqiao Wang, Qiqing Wang, Sien Lin, Gang Li, Benguo Wang, Jingqin Chen, Peng Zhang
Summary: This study presents a genetically engineered biomimetic membrane-coated mRNA (MR@P-mPTEN) carrier that effectively delivers mRNA-PTEN directly to the rheumatoid arthritis (RA) joint. The carrier is prepared by overexpressing tumor necrosis factor (TNF-α) receptors on macrophage biomimetic membranes, which serve as decoys to reduce inflammatory pathway activation. The results show that MR@P-mPTEN exhibits good stability, RA targeting, and competitive binding with TNF-α, activating the PTEN pathway to inhibit synovitis and joint damage.
Review
Cell Biology
William Hill, Deborah R. Caswell, Charles Swanton
Summary: Cancer undergoes clonal evolution with high intratumor heterogeneity, influenced by both internal and external factors. Advances in sequencing technology have enabled the creation of diverse mouse models to explore mechanisms of tumor evolution.
TRENDS IN CELL BIOLOGY
(2021)
Review
Oncology
Ryo Shiraishi, Daisuke Kawauchi
Summary: Recent studies have classified medulloblastoma into four molecular subgroups, each with distinct oncogenic drivers and cellular origins. Understanding the molecular signals driving tumorigenesis is still lacking, necessitating further research into the etiology of tumors to establish effective treatment strategies.
Review
Oncology
Yuriko Saiki, Can Jiang, Masaki Ohmuraya, Toru Furukawa
Summary: Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive malignancy and a major cause of cancer-related deaths worldwide. Recent multi-gene analysis methods have provided valuable insight into the molecular characteristics of pancreatic tumors, with different types of pancreatic cancer and precursor lesions showing specific molecular alterations. Genetically engineered mouse models (GEMMs) driven by oncogenic Kras have proven to be useful in understanding the roles of altered genes and recapitulating key features of human PDAC.
Review
Biochemistry & Molecular Biology
Niat T. Gebru, Shannon E. Hill, Laura J. Blair
Summary: FK506 binding protein 51 (FKBP51) is a molecular chaperone involved in stress response and various biological processes. Genetic and epigenetic alterations in the FKBP5 gene are associated with neuropsychiatric disorders. Animal models, such as mouse models, have played a crucial role in understanding the function of FKBP51. This review examines different mouse models of FKBP5, discussing their generation, observations, and potential implications for stress-related disorders.
JOURNAL OF CELLULAR BIOCHEMISTRY
(2023)
Article
Oncology
Weiping Li, Michael M. Shen
Summary: Prostate adenocarcinoma lacks distinguishable histopathological subtypes, but prostate cancer displays significant inter- and intratumor heterogeneity at the molecular level. Understanding the basis for prostate cancer heterogeneity can help distinguish aggressive from indolent disease, and help overcome treatment resistance in advanced prostate cancer.
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Johannes C. van der Mijn, Kristian B. Laursen, Leiping Fu, Francesca Khani, Lukas E. Dow, Dawid G. Nowak, Qiuying Chen, Steven S. Gross, David M. Nanus, Lorraine J. Gudas
Summary: Genetically engineered mouse models (GEMMs) are important for cancer research and therapy development. In this study, researchers developed two GEMMs using inducible CRISPR-Cas9 systems to simulate common chromosome deletions in kidney cancer. The models induced somatic mutations in the kidneys but did not cause tissue transformation. Further research is needed.
SCIENTIFIC REPORTS
(2023)
Review
Cell Biology
Rene Winkler, Eva-Maria Piskor, Christian Kosan
Summary: Oncogenic overexpression of MYC results in the dysregulation of signaling pathways, cellular metabolism, and cell growth, particularly in non-Hodgkin B-cell lymphomas. Genetically engineered mouse models (GEMMs), specifically E mu-Myc transgenic mice, have been developed to better understand MYC-induced B-cell lymphomagenesis. This study highlights the advantages of using E mu-Myc transgenic mice and discusses common challenges faced when using these mouse models. Additionally, the study provides an overview of the pathways affected by MYC based on knowledge gained from GEMMs and identifies key regulators of MYC-induced lymphomagenesis, including potential targets for future pharmacological interventions.
Article
Biophysics
Yujin Lee, Sung-Jo Kim, Ye-Ji Kim, You Hwan Kim, Ji-Young Yoon, Jonghyun Shin, Soo-Min Ok, Eun-Jung Kim, Eun Jung Choi, Jin-Woo Oh
Summary: This paper presents an efficient method for optimizing an M13 bacteriophage-based multi-array colorimetric sensor, which can classify multiple targets simultaneously. The sensor achieved high accuracy in classifying apple varieties, standard fragrances, and aging. This optimization technique has potential applications in medical diagnosis, hazardous environment monitoring, and the food industry.
BIOSENSORS & BIOELECTRONICS
(2023)
Review
Biochemistry & Molecular Biology
Megan N. Michalski, Bart O. Williams
Summary: This review discusses the development and application of genetically engineered mouse models in musculoskeletal biology research, with a particular focus on tissue-specific gene knockout and the impact of CRISPR/Cas technology.
Review
Biochemistry & Molecular Biology
Delf-Magnus Kummerfeld, Carsten A. Raabe, Juergen Brosius, Dingding Mo, Boris Skryabin, Timofey S. Rozhdestvensky
Summary: PWS is a neurogenetic disorder caused by the deletion of paternally imprinted genes on chromosome 15q11-q13, with mouse models playing a crucial role in understanding the syndrome's pathogenesis. Through murine models, the contribution of each affected gene to PWS has been unveiled, shedding light on the importance of non-protein coding genes in the locus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
G. F. Senguel, R. Mishra, E. Candiello, P. Schu
Summary: AP2 forms AP2 CCV with clathrin and other coat proteins, and synapses contain different types of CCV. The stability and composition of CCV are regulated by various factors, including Hsc70 and phosphorylation patterns. The knockout of the AP1/O1B complex disrupts synaptic vesicle recycling and endosomal protein sorting, leading to upregulation of endocytosis. Stable CCV, termed stCCV, have distinct characteristics and specialized functions in synaptic plasticity. The phosphorylation of Hsc70 and the levels of kinases play a crucial role in regulating the stability and disassembly of clathrin in CCV.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Martin Fluck, Colline Sanchez, Vincent Jacquemond, Christine Berthier, Marie-Noelle Giraud, Daniel Jacko, Kathe Bersiner, Sebastian Gehlert, Guus Baan, Richard T. Jaspers
Summary: Enhancing CaMKII signaling improves fatigue resistance and contractile characteristics of skeletal muscle by enhancing calcium release.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Letter
Biochemistry & Molecular Biology
Federica Coppola, Sara Monaci, Alessandro Falsini, Carlo Aldinucci, Irene Filippi, Daniela Rossi, Fabio Carraro, Antonella Naldini
Summary: The adaptor protein p62 plays a crucial role in maintaining the survival of dendritic cells (DCs) under hypoxic conditions by preserving Erk1/2 phosphorylation and reducing AMPK activation, thus extending their lifespan to ensure their functions in hypoxic microenvironments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Jenifer Pendiuk Goncalves, Jorvani Cruz Villarreal, Sierra A. Walker, Xuan Ning Sharon Tan, Chad Borges, Joy Wolfram
Summary: This study used a mass spectrometry-based approach to assess the differences in glycan features between extracellular vesicles (EVs) and originating cells. The results showed that EVs selectively enriched specific glycan features, particularly those associated with binding to the extracellular matrix. The study also found differences in EV glycan sorting between different metastatic cell lines and mouse models, indicating a potential role of glycan diversity in the metastatic process.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
De-ao Gong, Peng Zhou, Wen-yi Chang, Jia-yao Yang, Yan-lai Zhang, Ai-long Huang, Ni Tang, Kai Wang
Summary: Liver cancer, ranked sixth globally, is a major contributor to cancer-related mortality. Metastasis is the main cause of treatment failure and deaths in liver cancer. The SPOP-CREB5-MET axis plays a significant role in liver cancer metastasis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ning Huang, Jun Tang, Xiaoyao Yi, Maoxin Zhang, Bin Li, Yuan Cheng, Jin Chen
Summary: This study reveals that glioma-derived S100A9 can induce microglial M2 polarization, inhibit CD8+ T lymphocytes, and promote immunosuppression. The mechanism is related to the interaction with alpha v133 integrin and subsequent activation of AKT1 in microglia. The expression of S100A9 is positively associated with CD206 expression and negatively correlated with CD8+ T lymphocyte accumulation in the TME, suggesting a potential role of S100A9 in regulating the tumor microenvironment and immune evasion in glioma.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Yomna S. Abd El-Aziz, Matthew J. McKay, Mark P. Molloy, Betty McDowell, Elizabeth Moon, Loretta Sioson, Amy Sheen, Angela Chou, Anthony J. Gill, Patric J. Jansson, Sumit Sahni
Summary: This study identified a novel combination of autophagy inhibitors that can effectively inhibit the proliferation of oral squamous cell carcinoma (OSCC) cells, including both chemosensitive and chemoresistant cells. This research is important for the development of new therapies for advanced OSCC tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Luojia Liu, Xiaoqiang Liu, Ying Chen, Meng Kong, Jinghong Zhang, Min Jiang, Hongling Zhou, Jinrui Yang, Xu Chen, Ze Zhang, Chao Wu, Xupin Jiang, Jiaping Zhang
Summary: Our study revealed that the Paxillin/HDAC6 signaling pathway regulates microtubule acetylation in electric field-guided keratinocyte migration.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Julia Weikum, Jeroen F. van Dyck, Saranya Subramani, David P. Klebl, Merete Storflor, Stephen P. Muench, Soren Abel, Frank Sobott, J. Preben Morth
Summary: The study reveals the complex interaction between bacterial magnesium transporter A (MgtA) and cardiolipin 18:1 and cardiolipin 16:0, highlighting the importance of lipid environment in protein activity and stability. Further understanding of Mg2+ homeostasis in bacteria will provide insights into bacterial infections.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Sumit Kinger, Yuvraj Anandrao Jagtap, Ankur Rakesh Dubey, Prashant Kumar, Akash Choudhary, Rohan Dhiman, Vijay Kumar Prajapati, Deepak Chitkara, Krishna Mohan Poluri, Amit Mishra
Summary: Efficient protein synthesis and quality control mechanisms are crucial for maintaining proteostasis and preventing neurodegeneration. This study demonstrates that treating cells with Lanosterol can enhance the proteolytic activity of Proteasome and promote the removal of misfolded proteins, suggesting a potential therapeutic approach for abnormal protein accumulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Karolina Stepien, Adrianna Skoneczna, Monika Kula-Maximenko, Lukasz Jurczyk, Mateusz Molon
Summary: The replication of DNA requires a complex machinery called the replisome, which is highly conserved across species. One crucial component of the replisome is the CMG helicase complex, which unwinds DNA and coordinates the assembly and function of other replisome components. In this study, the impact of the absence of one copy of the CMG complex genes on the physiology and aging of yeast cells was investigated. The findings showed disruptions in the cell cycle, extended doubling times, and alterations in the biochemical profile of these cells. Importantly, it was found that heterozygous cells for CMG helicase genes exhibited increased reproductive potential and delayed aging. The study also highlighted potential therapeutic targets for cancer treatment using yeast.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Nishadh Rathod, Guadalupe Guerrero-Serna, Howard S. Young, L. Michel Espinoza-Fonseca
Summary: This study reveals that replacing Lys27 with Asn enhances the inhibitory potency of MLN without affecting SERCA's affinity for Ca2+. The findings suggest that the SERCA site modulating Ca2+ affinity also functions as a catalytic activity switch.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Can Jiang, Chunyang Zhang, Min Dai, Fuyan Wang, Sa Xu, Dan Han, Yanyan Wang, Yajie Cao, Yanyan Liang, Ziyu Zhang, Lina Yan, Yujun Shen, Kewu He, Yuxian Shen, Jun Liu
Summary: The phosphorylation of p65 and the expression of SUMO1 are increased in cancer tissues of HCC patients, and there is a positive correlation between SUMO1 and phosphorylated p65. SUMOylation of p65 by SUMO1 promotes p65 nuclear import and enhances NF-xB activity. Both SUMOylation and phosphorylation of p65 increase the viability and invasion of hepatoma cells, and decrease cell apoptosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ming-Fo Hsu, Yoshihiro Ito, Jai Prakash Singh, Shu-Fang Hsu, Alan Wells, Kuang-Yu Jen, Tzu-Ching Meng, Fawaz G. Haj
Summary: This study identified alpha-actinin4 as a novel substrate of PTP1B in podocytes and demonstrated their interaction in regulating podocyte function. Targeting PTP1B and alpha-actinin4 could be a potential therapeutic approach for podocyte injury.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Paulo F. V. Bizerra, Eduardo H. Gilglioni, Hang Lam Li, Simei Go, Ronald P. J. Oude Elferink, Arthur J. Verhoeven, Jung -Chin Chang
Summary: This study investigates the role of cyclic AMP (cAMP) in glycogen metabolism and reveals that cAMP regulates glycogenolysis in opposite directions depending on its site of synthesis within cells and downstream effectors. The canonical tmAC-cAMP-PKA signaling promotes glycogenolysis, while the non-canonical sAC-cAMP-Epac1 signaling suppresses glycogenolysis. This highlights the importance of cAMP microdomain organization for distinct metabolic regulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
