Article
Chemistry, Physical
Si Li, Liwei Xu, Meiru Lu, Maozhong Sun, Liguang Xu, Changlong Hao, Xiaoling Wu, Chuanlai Xu, Hua Kuang
Summary: In this study, chiral cobalt oxide nanoparticles stabilized with D- or L-cysteine were found to have no observable cytotoxicity and were internalized by dendritic cells through caveola-dependent endocytosis. The metabolic analysis showed that chiral L-NPs had a similar effect on bile acids in the liver as D-NPs, indicating that low chiroptical activity nanomaterials do not significantly impact metabolism and kinetics under physiological conditions.
Article
Nanoscience & Nanotechnology
Chunyan Liao, Xiang Wang, Xueying Zhou, Dequan Wang, Ziyin Zhang, Yan Liu, Xiao Wu, Ying Chen, Yifeng Tan, Xin Dai, Pei Jing, Jie Pang, Xiao Xiao, Jie Liu, Xiaoming Liao, Shiyong Zhang
Summary: Researchers have developed a nanodrug, VC@cLAV, which utilizes vitamin C (VC) and lipoic acid (LA) to efficiently kill cancer cells while protecting noncancer cells. The drug generates reactive oxygen species in cancer cells, leading to their destruction, while acting as an antioxidant in noncancer cells to prevent and repair oxidative stress-induced damage. Compared to traditional cytotoxic drugs, VC@cLAV demonstrates superior therapeutic outcomes and lacks side effects in various tumor models.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biochemistry & Molecular Biology
Lucia Palumbo, Salvatore Genovese, Chiara Collevecchio, Francesco Epifano, Serena Fiorito
Summary: This manuscript investigates the biomolecular mechanism of action of 4'-geranyloxyferulic acid, a natural colon cancer chemopreventive agent, in cultured Caco-2 cells. The application of this phytochemical leads to a time- and dose-dependent decrease in cell viability, accompanied by the generation of reactive oxygen species and induction of caspases 3 and 9, ultimately causing apoptosis. Key pro-apoptotic targets, including CD95, DR4 and 5, cytochrome c, Apaf-1, Bcl-2, and Bax, undergo significant modifications, explaining the observed apoptosis in Caco-2 cells treated with 4'- geranyloxyferulic acid.
Article
Chemistry, Multidisciplinary
Maryam Razmjooei, Seyed Mohammad Hashem Hosseini, Gholamhossein Yousefi, Mohammad-Taghi Golmakani, Mohammad Hadi Eskandari
Summary: The oral administration of insulin has been hindered by barriers in the gastrointestinal tract, resulting in low bioavailability. This study introduces a novel nanocarrier platform to address these challenges. The modified alginate nanoparticles showed high insulin loading and encapsulation efficiency, as well as sustained release and enhanced cellular uptake compared to insulin solution. The findings suggest that deoxycholic acid-modified alginate nanoparticles could be a promising option for overcoming gastrointestinal obstacles.
PHARMACEUTICAL RESEARCH
(2023)
Article
Pathology
Saran Lotfollahzadeh, Dominic Lo, Emily A. York, Marc A. Napoleon, Wenqing Yin, Nagla Elzinad, John Le, Mengwei Zhang, Xiaosheng Yang, Austin Morrissey, Murad Elsadawi, Qing Zhao, Scott E. Schaus, Ulla Hansen, Vipul C. Chitalia
Summary: This study reveals the Wnt inhibitory and CRC growth-suppressive effects of LSF inhibitors in CRC cells, providing a novel target in CRC therapeutics.
AMERICAN JOURNAL OF PATHOLOGY
(2022)
Review
Medicine, Research & Experimental
Akanksha Esmeeta, Subhamay Adhikary, V Dharshnaa, P. Swarnamughi, Z. Ummul Maqsummiya, Antara Banerjee, Surajit Pathak, Asim K. Duttaroy
Summary: Colon cancer, the third most common cancer, can be inhibited by plant-based compounds through various mechanisms. However, there are challenges in using these compounds, such as poor solubility, absorption, and intellectual property issues. Traditional medicines have advantages and limitations in treating cancer. This review focuses on the benefits and challenges of using bioactive compounds derived from plants for colon cancer.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Review
Biochemistry & Molecular Biology
Martina Cizmarikova, Radka Michalkova, Ladislav Mirossay, Gabriela Mojzisova, Martina Zigova, Annamaria Bardelcikova, Jan Mojzis
Summary: This review summarizes the role of ellagic acid (EA) as a potential therapeutic agent in cancer prevention and treatment by targeting key hallmarks of cancer. The multifaceted actions of EA make it a promising candidate for cancer prevention and therapy.
Article
Multidisciplinary Sciences
Simoun Icho, Jennifer S. Ward, John Tam, Larry K. Kociolek, Casey M. Theriot, Roman A. Melnyk
Summary: This study investigated the impact of the total pool of intestinal bile acids on the virulence of Clostridioides difficile. It was found that the amount of bile acids in the intestine is correlated with the ability to inhibit TcdB toxicity. Even in antibiotic-treated and germ-free mice, bile acids were able to protect cells from TcdB damage, but this protection could be overcome at high toxin doses typical of severe infections. These findings reveal the role of intestinal bile acids in attenuating virulence and provide insights into asymptomatic carriage of toxigenic C. difficile, as well as strategies for manipulating bile acid levels for therapeutic benefit.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Ga-Eun Lee, Cheol-Jung Lee, Hyun-Jung An, Han Chang Kang, Hye Suk Lee, Joo Young Lee, Sei-Ryang Oh, Sung-Jun Cho, Dae Joon Kim, Yong-Yeon Cho
Summary: Fargesin inhibits cancer cell growth by suppressing cell cycle transition and EGF-induced cell transformation through multiple signaling pathways, including CDK/cyclin and Rb-E2F pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Malgorzata Klosek, Dagmara Jaworska, Grazyna Pietsz, Ewelina Szliszka
Summary: This study examined the apoptotic effect of santin in combination with TRAIL on colon cancer cells. Flow cytometry and fluorescence microscopy were used to observe apoptosis and death receptor expression. It was found that santin synergized with TRAIL to induce apoptosis in colon cancer cells. This synergistic effect was achieved by increasing the expression of death receptors TRAIL-R1 and TRAIL-R2 and altering the mitochondrial membrane potential.
Article
Medicine, Research & Experimental
Alejandro Lopez-Mejia, Luis Gerardo Ortega-Perez, Oliver Rafid Magana-Rodriguez, Luis Alberto Ayala-Ruiz, Jonathan Saul Pinon-Simental, Daniel Godinez Hernandez, Patricia Rios-Chavez
Summary: The herbal preparation of Callistemon citrinus leaves showed chemopreventive efficacy against oxidative stress in colorectal cancer, maintaining or increasing antioxidant enzyme activities and reducing lipid peroxidation products levels. It also exhibited protective effects on damaged organs and induced the antioxidant system, making it a promising candidate for prevention and treatment of diseases like colorectal cancer.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Cell Biology
Guanlin Li, Yongqing Wang, Guangming Cao, Yeling Ma, Yu-Xia Li, Yangyu Zhao, Xuan Shao, Yan-Ling Wang
Summary: This study reveals that prolonged hypoxic stress induces increased endocytosis and repression of ubiquitin-mediated Met degradation, leading to impaired regulation of trophoblast invasion by HGF/Met signaling. These findings provide novel evidence for elucidating the pathogenesis of preeclampsia, especially of the early-onset subtype.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Article
Gastroenterology & Hepatology
Elham Kharazmi, Dominique Scherer, Felix Boekstegers, Qunfeng Liang, Kristina Sundquist, Jan Sundquist, Mahdi Fallah, Justo Lorenzo Bermejo
Summary: This study found an association between gallstones and an increased risk of kidney cancer after cholecystectomy. The risk of cancer was highest within 6 months after the surgery. The study emphasizes the importance of screening for kidney cancer before and after gallbladder removal.
Article
Oncology
Montana T. Morris, Abhishek Jain, Boshi Sun, Vadim Kurbatov, Engjel Muca, Zhaoshi Zeng, Ying Jin, Jatin Roper, Jun Lu, Philip B. Paty, Caroline H. Johnson, Sajid A. Khan
Summary: There are well demonstrated differences in tumor cell metabolism between right sided (RCC) and left sided (LCC) colon cancer, which could explain the robust differences observed in their clinical behavior, particularly in metastatic disease. Through metabolomics and transcriptomics analysis, we identified several key differences in cellular physiology between RCC and LCC liver metastasis that may contribute to clinical differences in tumor behavior.
Review
Biochemistry & Molecular Biology
Jung Yoon Jang, Eunok Im, Yung Hyun Choi, Nam Deuk Kim
Summary: Bile acids are important in lipid digestion and absorption, and have been found to be tumor suppressors. This review comprehensively summarizes the literature on bile acid-induced programmed cell death and the development of synthetic bile acid derivatives for cancer treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
G. F. Senguel, R. Mishra, E. Candiello, P. Schu
Summary: AP2 forms AP2 CCV with clathrin and other coat proteins, and synapses contain different types of CCV. The stability and composition of CCV are regulated by various factors, including Hsc70 and phosphorylation patterns. The knockout of the AP1/O1B complex disrupts synaptic vesicle recycling and endosomal protein sorting, leading to upregulation of endocytosis. Stable CCV, termed stCCV, have distinct characteristics and specialized functions in synaptic plasticity. The phosphorylation of Hsc70 and the levels of kinases play a crucial role in regulating the stability and disassembly of clathrin in CCV.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Martin Fluck, Colline Sanchez, Vincent Jacquemond, Christine Berthier, Marie-Noelle Giraud, Daniel Jacko, Kathe Bersiner, Sebastian Gehlert, Guus Baan, Richard T. Jaspers
Summary: Enhancing CaMKII signaling improves fatigue resistance and contractile characteristics of skeletal muscle by enhancing calcium release.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Letter
Biochemistry & Molecular Biology
Federica Coppola, Sara Monaci, Alessandro Falsini, Carlo Aldinucci, Irene Filippi, Daniela Rossi, Fabio Carraro, Antonella Naldini
Summary: The adaptor protein p62 plays a crucial role in maintaining the survival of dendritic cells (DCs) under hypoxic conditions by preserving Erk1/2 phosphorylation and reducing AMPK activation, thus extending their lifespan to ensure their functions in hypoxic microenvironments.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Jenifer Pendiuk Goncalves, Jorvani Cruz Villarreal, Sierra A. Walker, Xuan Ning Sharon Tan, Chad Borges, Joy Wolfram
Summary: This study used a mass spectrometry-based approach to assess the differences in glycan features between extracellular vesicles (EVs) and originating cells. The results showed that EVs selectively enriched specific glycan features, particularly those associated with binding to the extracellular matrix. The study also found differences in EV glycan sorting between different metastatic cell lines and mouse models, indicating a potential role of glycan diversity in the metastatic process.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
De-ao Gong, Peng Zhou, Wen-yi Chang, Jia-yao Yang, Yan-lai Zhang, Ai-long Huang, Ni Tang, Kai Wang
Summary: Liver cancer, ranked sixth globally, is a major contributor to cancer-related mortality. Metastasis is the main cause of treatment failure and deaths in liver cancer. The SPOP-CREB5-MET axis plays a significant role in liver cancer metastasis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ning Huang, Jun Tang, Xiaoyao Yi, Maoxin Zhang, Bin Li, Yuan Cheng, Jin Chen
Summary: This study reveals that glioma-derived S100A9 can induce microglial M2 polarization, inhibit CD8+ T lymphocytes, and promote immunosuppression. The mechanism is related to the interaction with alpha v133 integrin and subsequent activation of AKT1 in microglia. The expression of S100A9 is positively associated with CD206 expression and negatively correlated with CD8+ T lymphocyte accumulation in the TME, suggesting a potential role of S100A9 in regulating the tumor microenvironment and immune evasion in glioma.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Yomna S. Abd El-Aziz, Matthew J. McKay, Mark P. Molloy, Betty McDowell, Elizabeth Moon, Loretta Sioson, Amy Sheen, Angela Chou, Anthony J. Gill, Patric J. Jansson, Sumit Sahni
Summary: This study identified a novel combination of autophagy inhibitors that can effectively inhibit the proliferation of oral squamous cell carcinoma (OSCC) cells, including both chemosensitive and chemoresistant cells. This research is important for the development of new therapies for advanced OSCC tumors.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Luojia Liu, Xiaoqiang Liu, Ying Chen, Meng Kong, Jinghong Zhang, Min Jiang, Hongling Zhou, Jinrui Yang, Xu Chen, Ze Zhang, Chao Wu, Xupin Jiang, Jiaping Zhang
Summary: Our study revealed that the Paxillin/HDAC6 signaling pathway regulates microtubule acetylation in electric field-guided keratinocyte migration.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Julia Weikum, Jeroen F. van Dyck, Saranya Subramani, David P. Klebl, Merete Storflor, Stephen P. Muench, Soren Abel, Frank Sobott, J. Preben Morth
Summary: The study reveals the complex interaction between bacterial magnesium transporter A (MgtA) and cardiolipin 18:1 and cardiolipin 16:0, highlighting the importance of lipid environment in protein activity and stability. Further understanding of Mg2+ homeostasis in bacteria will provide insights into bacterial infections.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Sumit Kinger, Yuvraj Anandrao Jagtap, Ankur Rakesh Dubey, Prashant Kumar, Akash Choudhary, Rohan Dhiman, Vijay Kumar Prajapati, Deepak Chitkara, Krishna Mohan Poluri, Amit Mishra
Summary: Efficient protein synthesis and quality control mechanisms are crucial for maintaining proteostasis and preventing neurodegeneration. This study demonstrates that treating cells with Lanosterol can enhance the proteolytic activity of Proteasome and promote the removal of misfolded proteins, suggesting a potential therapeutic approach for abnormal protein accumulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Karolina Stepien, Adrianna Skoneczna, Monika Kula-Maximenko, Lukasz Jurczyk, Mateusz Molon
Summary: The replication of DNA requires a complex machinery called the replisome, which is highly conserved across species. One crucial component of the replisome is the CMG helicase complex, which unwinds DNA and coordinates the assembly and function of other replisome components. In this study, the impact of the absence of one copy of the CMG complex genes on the physiology and aging of yeast cells was investigated. The findings showed disruptions in the cell cycle, extended doubling times, and alterations in the biochemical profile of these cells. Importantly, it was found that heterozygous cells for CMG helicase genes exhibited increased reproductive potential and delayed aging. The study also highlighted potential therapeutic targets for cancer treatment using yeast.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Nishadh Rathod, Guadalupe Guerrero-Serna, Howard S. Young, L. Michel Espinoza-Fonseca
Summary: This study reveals that replacing Lys27 with Asn enhances the inhibitory potency of MLN without affecting SERCA's affinity for Ca2+. The findings suggest that the SERCA site modulating Ca2+ affinity also functions as a catalytic activity switch.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Can Jiang, Chunyang Zhang, Min Dai, Fuyan Wang, Sa Xu, Dan Han, Yanyan Wang, Yajie Cao, Yanyan Liang, Ziyu Zhang, Lina Yan, Yujun Shen, Kewu He, Yuxian Shen, Jun Liu
Summary: The phosphorylation of p65 and the expression of SUMO1 are increased in cancer tissues of HCC patients, and there is a positive correlation between SUMO1 and phosphorylated p65. SUMOylation of p65 by SUMO1 promotes p65 nuclear import and enhances NF-xB activity. Both SUMOylation and phosphorylation of p65 increase the viability and invasion of hepatoma cells, and decrease cell apoptosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Ming-Fo Hsu, Yoshihiro Ito, Jai Prakash Singh, Shu-Fang Hsu, Alan Wells, Kuang-Yu Jen, Tzu-Ching Meng, Fawaz G. Haj
Summary: This study identified alpha-actinin4 as a novel substrate of PTP1B in podocytes and demonstrated their interaction in regulating podocyte function. Targeting PTP1B and alpha-actinin4 could be a potential therapeutic approach for podocyte injury.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Biochemistry & Molecular Biology
Paulo F. V. Bizerra, Eduardo H. Gilglioni, Hang Lam Li, Simei Go, Ronald P. J. Oude Elferink, Arthur J. Verhoeven, Jung -Chin Chang
Summary: This study investigates the role of cyclic AMP (cAMP) in glycogen metabolism and reveals that cAMP regulates glycogenolysis in opposite directions depending on its site of synthesis within cells and downstream effectors. The canonical tmAC-cAMP-PKA signaling promotes glycogenolysis, while the non-canonical sAC-cAMP-Epac1 signaling suppresses glycogenolysis. This highlights the importance of cAMP microdomain organization for distinct metabolic regulation.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)