Article
Cell Biology
Daren Elkrief, Yu-Shu Cheng, Oleg S. Matusovsky, Dilson E. Rassier
Summary: The interaction between actin and myosin is crucial for muscle contraction and force production. Oxidation of actin and myosin has been found to weaken the myofibrils in healthy and diseased muscles. This study shows that exposure to a chemical compound can affect the interactions between actin and myosin, leading to decreased velocity and force. Oxidation of both actin and myosin contributes to a decrease in contractile activity in muscles.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Alf Mansson, Dilson E. Rassier
Summary: Bottom-up mechanokinetic models predict ensemble function of actin and myosin based on parameter values derived from studies using isolated proteins. This study tests the prediction capability of a model for different drugs and concentrations, and finds that it qualitatively predicts the effects of most drugs but quantitatively fails to account for the effects of one specific drug. By adjusting key model parameters, the modified model not only improves the prediction of the effects of the specific drug, but also maintains good prediction accuracy for normal physiological ensemble function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Physiology
Walter Herzog
Summary: This article introduces the methods, applications, advantages, and disadvantages of single sarcomere/myofibril research, emphasizing its importance in uncovering the mechanisms of muscle contraction.
FRONTIERS IN PHYSIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
K. W. Ranatunga, M. A. Geeves
Summary: Findings from experiments using hydrostatic pressure changes show that the force in resting muscle is insensitive to pressure increase, while the force in rigour muscle increases. High pressure leads to tension potentiation in submaximal contractions. However, the force in maximally activated muscle decreases with increased pressure, and this decrease is sensitive to the concentration of ATP hydrolysis products. Rapid decrease in hydrostatic pressure results in force recovery. Understanding the underlying mechanisms of tension potentiation and muscle fatigue can be achieved through pressure experiments on intact muscle.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yinna Wang, Guoning Liao, Ruping Wang, Dale D. Tang
Summary: Actin cytoskeletal reorganization is crucial for regulating smooth muscle contraction, with Abi1 playing a key role in this process through acetylation at K416. The interaction between Abi1 and N-WASP enhances actin polymerization, ultimately promoting smooth muscle contraction. Additionally, the localization of p300 in the cytoplasm of smooth muscle cells suggests a novel mechanism for the acetylation-dependent regulation of the actin cytoskeleton.
Article
Cell Biology
Shiri Avivi Kela, Kriti Sethi, Pei Yi Tan, Danesha Suresh, Hui Ting Ong, Perla G. Castaneda, Mustafi R. Amin, Tal Laviv, Erin J. Cram, Jan Faix, Ronen Zaidel-Bar
Summary: This study identified a novel mechanotransduction pathway in the C. elegans spermatheca, which involves tension-dependent localization of a RhoGEF and a positive feedback loop for RHO-1-MyosinII activation. It provides insights into how epithelial tubes can sense and adjust their contractility in response to internal pressure.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Cell Biology
Michaeljohn Kalakoutis, Ross D. D. Pollock, Norman R. R. Lazarus, R. Andrew Atkinson, Marc George, Onur Berber, Roger C. C. Woledge, Julien Ochala, Stephen D. R. Harridge
Summary: The specific force (SF) of human aging in single muscle fibers is inconsistent in different studies, which may result from variations in the health status/physical activity levels and methodological differences. This study compared SF in fibers from older hip fracture patients, healthy master cyclists, and healthy nontrained young adults using two different activating solutions. The results showed that the activating solution significantly affected the force and revealed a difference in sensitivity in muscle fibers of hip fracture patients.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Natalia A. Koubassova, Andrey K. Tsaturyan
Summary: Contraction of cardiac muscle is regulated by Ca2+ ions via regulatory proteins, troponin (Tn), and tropomyosin (Tpm) associated with the thin (actin) filaments in myocardial sarcomeres. Recent cryo-electron microscopy (cryo-EM) models of the complex allow one to study the dynamic and mechanical properties of the complex using molecular dynamics (MD). However, further refinement of the models is needed to improve the protein-protein interaction in some regions of the complex, as suggested by problems revealed from the MD simulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Irene Pertici, Lorenzo Bongini, Marco Caremani, Massimo Reconditi, Marco Linari, Gabriella Piazzesi, Vincenzo Lombardi, Pasquale Bianco
Summary: The study analyzes the mechanical performances of vertebrate skeletal muscle during isometric and isotonic contractions, and their corresponding energy consumption, to define the coupling between mechanical and biochemical steps in the myosin-actin energy transduction cycle. The emergent properties of a simplified synthetic nanomachine, consisting of eight HMM molecules and an actin filament, are also assessed. The study predicts the relationships between energetic and mechanical properties of fast skeletal muscle, both in vivo and in vitro, through a three-state model of myosin-actin interaction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biophysics
Walter Herzog, Gudrun Schappacher-Tilp
Summary: Studies of muscle structure and function have a long history, with the modern era of muscle contraction mechanisms starting in the 1950s. AF Huxley and HE Huxley, both born in the UK and working independently, made significant contributions to understanding muscle contraction. AF Huxley developed the cross-bridge model of muscle contraction, which is still widely used today, while HE Huxley proposed the sliding filament theory.
JOURNAL OF BIOMECHANICS
(2023)
Review
Biochemistry & Molecular Biology
Denis Noble
Summary: The 1968 review article accurately identified the major steps in muscle contraction and successfully identified the role of troponin, inspiring mathematical models of muscle activity to this day.
PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Josh Baker
Summary: Most muscle contraction models in literature are molecular power stroke models, but they contradict centuries of science, 85 years of unrefuted evidence, and a quarter century of observations that suggest muscle contraction is a molecular switch mechanism. The use of these models has muddled our understanding of muscle function and it is time to hold proponents accountable and test both corpuscular and thermodynamic mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Luisa Moretto, Marko Usaj, Oleg Matusovsky, Dilson E. Rassier, Ran Friedman, Alf Mansson
Summary: Release of Pi from the myosin active site is essential for force generation, but the mechanism behind it is not well understood. This study presents evidence for a multistep Pi-release model that reconciles conflicting views and reveals potential functional complexities.
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Koichi Ojima, Masahiro Kigaki, Emi Ichimura, Takahiro Suzuki, Ken Kobayashi, Susumu Muroya, Takanori Nishimura
Summary: The dynamics of different isoforms of myosin in skeletal muscle have been studied using a mouse model expressing fluorescently tagged slow and fast myosin. The results showed that the response to protein turnover disturbance varied between slow and fast myofibers. These findings highlight the importance of studying endogenous myosin dynamics in different muscle fiber types.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Lauren E. Schultz, Mert Colpan, Garry E. Smith Jr, Rachel M. Mayfield, Tania M. Larrinaga, Alla S. Kostyukova, Carol C. Gregorio
Summary: Actin, a highly expressed protein in eukaryotic cells, plays a vital role in cellular processes. This study found that a mutation in the Lmod3 protein disrupts its structure and function, leading to the development of muscle diseases.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
A. Prabakaran, Amit Alexander
Summary: The molecular interactions and mucoadhesive nature of chitosan-coated liposomes with mucin are crucial for the development of an effective drug delivery system. The positively charged SA-CH-LPs showed stronger interaction and better mucoadhesive properties compared to negatively charged SALPs, thereby improving drug retention in the nasal cavity and enhancing therapeutic efficacy.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Maryam Noei-Khesht Masjedi, Esmaeil Sadroddiny, Jafar Ai, Saeed Balalaie, Yazdan Asgari
Summary: This study discovered an effective strategy for cargo sorting within exosomes by incorporating an appropriate cleavage site, providing further insight into the potential of exosomes as nano-shuttles bearing therapeutic biomolecules.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Pei -Gee Yap, Chee-Yuen Gan
Summary: This study collected the sequences of 128 tyrosinase inhibitory peptides and analyzed their hydrophobicity/hydrophilicity properties and amino acid profiles. Molecular docking analysis was used to investigate the binding interactions between peptides and tyrosinase. The study found that hydrophobic and/or polar neutral properties facilitate or stabilize peptide binding with tyrosinase, and short peptides with cysteine and tyrosine tend to bind to the active site of tyrosinase. These findings provide detailed explanations for the relationship between peptide/amino acid structures and tyrosinase inhibition, as well as potential anti-melanogenesis mechanisms for peptide-based treatments against skin hyperpigmentation.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Ye Yang, Hai-Lian Chen, Su Fang Wu, Wei Bao
Summary: The study found that CHMP4B and VPS4A play an important role in reversing GSDMD-mediated pyroptosis by facilitating cell membrane remodeling in endometrial carcinoma.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Aya Al Othman, Dmitry Bagrov, Julian M. Rozenberg, Olga Glazova, Gleb Skryabin, Elena Tchevkina, Alexandre Mezentsev, Mikhail Durymanov
Summary: Arc protein is found in glutamatergic neurons of vertebrates and can be transferred between neurons in extracellular vesicles (EVs). In glioma cells, Arc protein is also present in EVs and can facilitate the transfer of mRNA, potentially contributing to tumor progression and affecting synaptic plasticity in cancer patients.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Nan Zhang, Meng-yu Shen, Qing-li Meng, Hao-ping Sun, Fang-yi Fan, Hai Yi, Yong-jian Yang
Summary: In this study, it was demonstrated for the first time that FAT1 inhibited AML proliferation by reducing autophagy level. FAT1 achieved this by decreasing the expression of ATG4B, which is related to autophagy. Mechanistically, FAT1 decreased the levels of phosphorylated and intranuclear smad2/3, thus decreasing the activity of the ATG4B gene promoter. It was also found that FAT1 competitively bound to TGF-beta R II, leading to reduced phosphorylation of TGF-beta R I and smad2/3. Knockdown of FAT1 promoted AML autophagy and proliferation in vivo.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Oleh Demianchuk, Myroslava Vatashchuk, Dmytro Gospodaryov, Viktoria Hurza, Marian Ivanochko, Vitalii Derkachov, Vladyslav Berezovskyi, Oleh Lushchak, Kenneth B. Storey, Maria Bayliak, Volodymyr I. Lushchak
Summary: This study investigated the effects of a high-fat high-fructose diet (HFFD) on the behavior, energy metabolism, and oxidative stress markers in the cerebral cortex of mice. The results showed that HFFD stimulated locomotion and defecation, while an AKG-supplemented diet had a tendency to promote anxiety-like behavior. Additionally, there were significant differences in glutathione-dependent detoxification and processes related to autophagy between the two diets.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Yusaku Chukai, Ginga Ito, Yasuo Miki, Koichi Wakabayashi, Ken Itoh, Eriko Sugano, Hiroshi Tomita, Tomokazu Fukuda, Taku Ozaki
Summary: The study found that mitochondrial calpain-5 plays an important role in the occurrence and development of ischemia-reperfusion injury and is expressed in the human and mouse brains. Targeting the expression or activity of mitochondrial calpain-5 may have significant implications for suppressing inflammation during I/R injuries such as cerebrovascular diseases.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Aline Dias da Purificaca, Victor Debbas, Leonardo Yuji Tanaka, Gabriele Veronica de Mello Gabriel, Joao Wosniak Junior, Tiphany Coralie De Bessa, Sheila Garcia-Rosa, Francisco Rafael Martins Laurindo, Percillia Victoria Santos Oliveira
Summary: The ER transmembrane chaperones DNAJB12 and DNAJB14 play important roles in protein folding and ER stress response. They are regulated by thiol redox processes and are involved in ER protein reflux.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Ekaterina O. Bryanskaya, Andrey Y. Vinokurov, Angelina I. Dolgikh, Andrey Dunaev, Plamena R. Angelova, Andrey Y. Abramov
Summary: FAD autofluorescence in cells can assess enzymatic activity, and its intensity variations may be related to different cell types and tissues. High levels of FAD autofluorescence can indicate cell pathology and potentially predict the occurrence of apoptosis and necrosis.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)
Article
Biochemistry & Molecular Biology
Rumei Luan, Manyu Luo, Dongyan Ding, Xin Su, Junling Yang
Summary: Zinc deficiency can worsen obesity-related lung damage, and Nrf2 activation is one of the important mechanisms of this protective effect. Regulating zinc homeostasis can contribute to the prevention and treatment of obesity-related lung injury.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2024)