Article
Biochemistry & Molecular Biology
Zhifeng Ye, Tingting Zhao, Qianhao Wei, Haoran Lin, Yong Zhang, Shuisheng Li
Summary: This study investigated the expression of vitellogenin and estrogen receptors in the liver of orange-spotted grouper. The results showed that their expression levels increased significantly during the breeding season and were upregulated by estradiol (E-2) injection. ER beta 2 was found to mediate the E-2-induced expression of vitellogenin and ER alpha, and the transcriptional activity of ER alpha was enhanced through a classical ERE-dependent pathway. This study provides insights into the roles of ER subtypes in VTG expression and the efficient VTG production in orange-spotted grouper during the breeding season.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Endocrinology & Metabolism
Jun Nagata, Yuji Mushirobira, Osamu Nishimiya, You Yamaguchi, Toshiaki Fujita, Naoshi Hiramatsu, Akihiko Hara, Takashi Todo
Summary: Estradiol-17 beta (E2) regulates transcription of estrogen-responsive genes via estrogen receptors (Esr). Different Esr subtypes were distinctly different in their ability to transactivate estrogen-responsive target genes, resulting in differential expression of chg, vtg and esr1a genes in the estrogen-exposed hepatocytes.
GENERAL AND COMPARATIVE ENDOCRINOLOGY
(2021)
Article
Environmental Sciences
Rucong Liu, Kang Li, Guixiang Wang, Zhixin Jiang, Xubing Ba, Liping Liu
Summary: This study explored the effect of swimming on the synthesis of VTG in conger eel. The results showed that flowing water can inhibit the expression of VTG and Er genes in the liver, reduce the synthesis of VTG, and inhibit yolk accumulation during ovarian development in conger eel.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Environmental Sciences
Rasmus Sogaard, Henrik Holbech, Ulrik Norum, Poul Bjerregaard
Summary: The study investigated the estrogenic activity of chemical UV-filters 4-MBC and OMC in rainbow trout through intraperitoneal injections. Results showed that 4-MBC increased plasma vitellogenin concentrations dose-dependently, while OMC did not show statistically significant responses in exposed fish groups. This confirms the estrogenic effects of 4-MBC in fish in vivo.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Fisheries
Pallath Muhammed Nuzaiba, Tincy Varghese, Subodh Gupta, Narottam Prasad Sahu, Prem Prakash Srivastava
Summary: The study showed that dietary genistein has an inducing effect on vitellogenesis in male carp, leading to enhanced estrogenic responses, increased testosterone levels, and reduced activity of steroidogenic enzymes, possibly causing a delay in maturation.
Article
Environmental Sciences
Diane A. Mielewczyk, Chris N. Glover, Chantelle E. Klaczek, Greg G. Goss, Gavin N. Saari
Summary: Fish mortality is linked to harmful algal blooms, but the relationship between toxicity, cyanotoxins, and water chemistry remains unclear. This study examined the impact of waterborne microcystin-LR exposure on rainbow trout and found that it specifically affected calcium metabolism without general effects on ion regulation. The mechanism underlying this effect is still unknown.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Fisheries
Masoumeh Darvishi, Roghieh Safari, Seyed Hossein Hoseinifar, Ali Shabani, Maryam Dadar, Zahra Jarayedi, Marina Paolucci
Summary: The study found that after treatment with sub-lethal doses of Diazion, the expression of reproductive-related genes in female zebrafish significantly decreased, while the expression of immune and antioxidant related genes increased considerably. Ovarian morphology showed a decrease in oocyte development in groups treated with Diazion. Understanding the function and mechanism of action of Diazion on the female reproductive, immune, and oxidant status may provide new strategies to counteract its adverse effects.
AQUACULTURE REPORTS
(2022)
Article
Biochemistry & Molecular Biology
S. Sreelekshmi, K. Manish, M. C. Subhash Peter, R. Moses Inbaraj
Summary: This study investigated the effects of hypoxic and osmotic stress on neurotransmitters levels in the brain of zebrafish, revealing alterations in serotonin, epinephrine, and norepinephrine levels. The stressors also impacted the expression of reproductive signal genes, suggesting a potential role of neurotransmitters in regulating responses to acute stress in zebrafish brain.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2022)
Article
Environmental Sciences
Michael Niederwanger, Josef Wieser, Melanie Thaler, Agnes Felber, Katja Schmoelz, Wolfgang Mark, Erich Tasser, Rudiger Kaufmann, Bernd Pelster
Summary: The efflux of organic contaminants from sewage treatment plants and extensive pesticide use in vineyards and orchards have detrimental effects on fish species, particularly their endocrine system. This study investigated the effects of sewage treatment plant efflux and pesticide use on selected fish species in Austrian freshwater streams. Biomarkers related to endocrine disruption were identified, and mRNA expression levels were measured. The results showed increased mRNA expression in the tested fish species, indicating the presence of endocrine disrupting chemicals. This study provides important insights for further research on the effects of these chemicals on fish populations.
FRONTIERS IN ENVIRONMENTAL SCIENCE
(2022)
Article
Engineering, Environmental
Alper James G. Alcaraz, Kamil Mikulasek, David Potesil, Bradley Park, Kamran Shekh, Jessica Ewald, Connor Burbridge, Zbynek Zdrahal, David Schneider, Jianguo Xia, Doug Crump, Niladri Basu, Markus Hecker
Summary: This study established an embryo benchmark dose assay for rainbow trout using EE2 as a model compound to derive transcriptomic points-of-departure (tPODs) as an alternative to live-animal tests. The results showed that the 4-day alternative RBT embryonic assay was effective in deriving tPODs that are protective of chronic effects of EE2.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jamie L. Mankiewicz, Beth M. Cleveland
Summary: Leptin, a cytokine that regulates appetite and energy expenditure, has been found to have two receptors, Lepra1 and Lepra2, in rainbow trout. These receptors show tissue-specific expression patterns and are differentially regulated during feed deprivation, indicating paralog- and tissue-specific functions in the leptin signaling pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Rodrigo Zuloaga, Daniela Aravena-Canales, Jorge Eduardo Aedo, Cesar Osorio-Fuentealba, Alfredo Molina, Juan Antonio Valdes
Summary: In aquaculture, cortisol is believed to perform glucocorticoid and mineralocorticoid functions in teleosts. However, recent data suggest that 11-deoxycorticosterone (DOC) released during stress events may also modulate the compensatory response. A transcriptomic analysis on rainbow trout revealed that DOC induced differentially expressed transcripts (DETs) mainly associated with muscle contraction, sarcomere organization, and cell adhesion. Further analysis showed that DOC's function in skeletal muscle stress response is differentially modulated by glucocorticoid receptor (GR) and mineralocorticoid receptor (MR), and is complementary to cortisol.
Article
Biochemistry & Molecular Biology
Cecile Heraud, Theo Hirschinger, Elodie Baranek, Laurence Larroquet, Anne Surget, Franck Sandres, Anthony Lanuque, Frederic Terrier, Jerome Roy
Summary: This study aimed to elucidate the role of the sensory olfactory pathways in the regulation of feeding behavior in carnivorous rainbow trout. The researchers found that a total plant-based diet had a significant impact on the growth performance and feed intake of the trout. Gene expression analysis showed that OLFC and MOR genes played important roles in olfactory sensing perception. Additionally, the plant-based diet affected olfactory sensing pathways and monoaminergic neurotransmission in brain areas related to olfactory-driven behaviors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Hui-Xia Yu, Yang Li, Wei-Jia Song, Hui Wang, Hao-Lin Mo, Qiao Liu, Xin-Miao Zhang, Ze-Bin Jiang, Li-Xin Wang
Summary: This study characterized the rainbow trout MC3R and found its close relation to mammalian MC3R. The transcripts of rtMC3R were highly expressed in the brain and muscle. rtMC3R can be activated by agonists and modulate multiple signal pathways, while inhibiting the NF-kappa B signaling pathway.
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Huiwon Seo, Hyeyeong Seo, Ha-yeon Lee, Seok-Hee Lee, Yooheon Park
Summary: This study evaluated the comprehensive activity of representative estrogen receptor (ER) ligands using BRET-based assays and provided information about receptor specificity and cellular estrogen signaling pathways. The results showed that various chemicals mediated ER alpha and ER8 dimerization, with some acting as estrogen agonists and others as antagonists. The study also supported the potential use of BRET assays as high-throughput screening methods for assessing the endocrine-disrupting activity of environmental agents.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Biochemistry & Molecular Biology
Romain Carmeille, Severine A. Degrelle, Laurent Plawinski, Flora Bouvet, Celine Gounou, Daniele Evain-Brion, Alain R. Brisson, Anthony Bouter
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2015)
Article
Biochemistry & Molecular Biology
Adelaide Le Grand, Anthony Bouter, Anne Couturier, Odile Mulner-Lorillon, Xavier Le Goff, Franck Chesnel, Olivier Sire, Veronique Le Tilly
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2015)
Article
Developmental Biology
A. Bouter, R. Carmeille, C. Gounou, F. Bouvet, S. A. Degrelle, D. Evain-Brion, A. R. Brisson
Article
Biochemistry & Molecular Biology
Romain Carmeille, Flora Bouvet, Sisareuth Tan, Coralie Croissant, Celine Gounou, Kamel Mamchaoui, Vincent Mouly, Alain R. Brisson, Anthony Bouter
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2016)
Meeting Abstract
Developmental Biology
Severine Degrelle, Pascale Gerbaud, Anthony Bouter, Jean Guibourdenche, Alain Brisson, Daniele Evain-Brion, Guillaume Pidoux
Meeting Abstract
Developmental Biology
Severine Degrelle, Pascale Gerbaud, Anthony Bouter, Jean Guibourdenche, Alain Brisson, Daniele Evain-Brion, Guillaume Pidoux
Meeting Abstract
Developmental Biology
Alain Brisson, Anthony Bouter, Romain Carmeille, Sisareuth Tan, Celine Gounou, Severine Degrelle, Guillaume Pidoux, Daniele Evain-Brion
Article
Multidisciplinary Sciences
Regine Dazzoni, Axelle Grelard, Estelle Morvan, Anthony Bouter, Christopher J. Applebee, Antoine Loquet, Banafshe Larijani, Erick J. Dufourc
SCIENTIFIC REPORTS
(2020)
Article
Cell Biology
Coralie Croissant, Celine Gounou, Flora Bouvet, Sisareuth Tan, Anthony Bouter
Article
Multidisciplinary Sciences
Aurelien Ducat, Betty Couderc, Anthony Bouter, Louise Biquard, Rajaa Aouache, Bruno Passet, Ludivine Doridot, Marie-Benoite Cohen, Pascale Ribaux, Clara Apicella, Irene Gaillard, Sophia Palfray, Yulian Chen, Alexandra Vargas, Amelie Jule, Leo Frelin, Julie Cocquet, Camino Ruano San Martin, Sebastien Jacques, Florence Busato, Jorg Tost, Celine Mehats, Paul Laissue, Jean-Luc Vilotte, Francisco Miralles, Daniel Vaiman
Article
Multidisciplinary Sciences
F. Bouvet, M. Ros, E. Bonedeau, C. Croissant, L. Frelin, F. Saltel, V. Moreau, A. Bouter
SCIENTIFIC REPORTS
(2020)
Review
Biochemistry & Molecular Biology
Coralie Croissant, Romain Carmeille, Charlotte Brevart, Anthony Bouter
Summary: Muscular dystrophies are genetic disorders characterized by weakening and loss of skeletal muscle mass. ANXA proteins are important for membrane repair in cells, and dysregulation of ANXA expression may impact the clinical severity of muscular dystrophies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Coralie Croissant, Celine Gounou, Flora Bouvet, Sisareuth Tan, Anthony Bouter
Summary: Defects in membrane repair contribute to the development of muscular dystrophies, and this study provides insights into the mechanism of membrane repair in healthy human skeletal muscle cells using correlative light and electron microscopy (CLEM). The results suggest that ANXA4 and ANXA6 are involved in sarcolemma remodeling and resealing, while ANXA1 and A2 contribute to membrane resealing by forming a lipid patch with intracellular vesicles.
Article
Cell Biology
Celine Gounou, Flora Bouvet, Benjamin Liet, Valerie Prouzet-Mauleon, Lena d'Agata, Etienne Harte, Francoise Argoul, Geraldine Siegfried, Richard Iggo, Abdel-Majid Khatib, Anthony Bouter
Summary: ANXA5 and ANXA6 are crucial components of the membrane repair machinery in MDA-MB-231 cells and are correlated with the invasiveness of breast cancer cells. High expression of ANXA5 and ANXA6 predicts poor prognosis in high-grade lung, gastric, and breast cancers. Genetic inhibition of ANXA5 and ANXA6 in zebrafish significantly reduces tumor cell dissemination.
BIOLOGY OF THE CELL
(2023)
Meeting Abstract
Biophysics
N. Harmouche, C. Courreges, A. Bouter, P. Bounafous, F. Nallet, N. Laurence, E. J. Dufourc
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2013)
Article
Biochemistry & Molecular Biology
Damien Marchese, Florent Guislain, Tamara Pringels, Laure Bridoux
Summary: Homopolymeric amino acid repeats are common in human proteins, particularly in transcription factors and kinases. This study focuses on homopolymeric histidine repeats (polyH) and their role in regulating embryonic development. Through bioinformatic analysis, the study identifies that polyH-containing proteins interact with cysteine-rich proteins and proteins containing cysteine repeats. The study further investigates the HOXA1 protein, a transcription factor with a long polyH motif, and finds that the polyH motif is necessary for its interaction with cysteine-rich proteins. Additionally, the study discovers that metal ions are required for the HOXA1-MDFI interaction and identifies three polyH interactors that down-regulate the transcriptional activity of HOXA1.
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2024)