Journal
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
Volume 1788, Issue 1, Pages 209-224Publisher
ELSEVIER
DOI: 10.1016/j.bbamem.2008.10.012
Keywords
Fluorescence; Lipid bilayer; Lipid raft; Membrane phase separation; Phase diagram
Categories
Funding
- FCT (Portugal) [PPCDT/QUI/57123/2004, PFDC/QUI/68151/2006, BPD/30289/2006]
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The application of Forster Resonance Energy Transfer (FRET) to the detection and characterization of phase separation in lipid bilayers (both in model systems and in cell membranes) is reviewed. Models describing the rate and efficiency of FRET for both uniform probe distribution and phase separation, and recently reported methods for detection of membrane heterogeneity and determination of phase boundaries, probe partition coefficients and domain size, are presented and critically discussed. Selected recent applications of FRET to one-phase lipid systems, gel/fluid phase separation, liquid ordered/liquid disordered phase separation (lipid rafts), complex systems containing ceramide and cell membranes are presented to illustrate the wealth of information that can be inferred from carefully designed FRET studies of membrane domains. (C) 2008 Elsevier B.V. All rights reserved.
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