Journal
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
Volume 1837, Issue 10, Pages 1602-1610Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbabio.2014.07.010
Keywords
Mitochondria; Fatty acids; Large-conductance calcium-activated potassium channel
Categories
Funding
- National Science Centre [2011/01/N/NZ/04311]
- Deutsches Zentrum fur Neurodegenerative Erkrankungen (DZNE)
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Polyunsaturated fatty acids (PUFAs) and their metabolites can modulate several biochemical processes in the cell and thus prevent various diseases. PUFAs have a number of cellular targets, including membrane proteins. They can interact with plasma membrane and intracellular potassium channels. The goal of this work was to verify the interaction between PUFAs and the most common and intensively studied mitochondrial large conductance Ca2+-regulated potassium channel (mitoBK(ca)). For this purpose human astrocytoma U87 MG cell lines were investigated using a patch-clamp technique. We analyzed the effects of arachidonic acid (AA); eicosatetraynoic acid (ETYA), which is a non-metabolizable analog of AA; docosahexaenoic acid (DHA); and eicosapentaenoic acid (EPA). The open probability (P-o) of the channel did not change significantly after application of 10 mu M ETYA. P-o increased, however, after adding 10 mu M AA. The application of 30 mu M DHA or 10 mu M EPA also increased the P-o of the channel. Additionally, the number of open channels in the patch increased in the presence of 30 mu M EPA. Collectively, our results indicate that PUFAs regulate the BKca channel from the inner mitochondria] membrane. (C) 2014 Elsevier B.V. All rights reserved.
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