Article
Physiology
Abel M. Mengeste, Natasa Nikolic, Andrea Dalmao Fernandez, Yuan Z. Feng, Tuula A. Nyman, Sander Kersten, Fred Haugen, Eili Tranheim Kase, Vigdis Aas, Arild C. Rustan, G. Hege Thoresen
Summary: This study used electrical pulse stimulation to investigate the metabolic effects of exercise in human skeletal muscle cells. The results showed enhanced oxidative metabolism in young male subjects. Analysis of the transcriptome, proteome, and secreted proteins revealed regulatory molecules and signaling pathways associated with glycolytic pathways, fatty acid oxidation, and muscle contraction. These findings provide valuable insights into the molecular mechanisms and beneficial metabolic effects of exercise.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Shufang Cai, Tianqi Duo, Xiaoyu Wang, Xian Tong, Chenglong Luo, Yaosheng Chen, Jianhao Li, Delin Mo
Summary: Studies have shown that pigs with low meat production exhibit more intense muscle generation during early embryonic development compared to pigs with high meat production. Metabolomic analysis revealed that nucleotide metabolism and energy metabolism were increased in Lantang pigs, suggesting a rapid synthesis of nucleic acids and ATP to support cell proliferation and differentiation. These findings provide insights into the molecular mechanisms underlying skeletal muscle development in different pig breeds.
Article
Biochemistry & Molecular Biology
James R. Krycer, Mary Lor, Rebecca L. Fitzsimmons, James E. Hudson
Summary: Complex diseases are linked to changes in metabolism, and a new method using a bicarbonate-buffered well-plate platform has been developed to study and screen metabolic activity under more physiological conditions.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Andrea Dalmao-Fernandez, Aleksandra Aizenshtadt, Hege G. G. Bakke, Stefan Krauss, Arild C. C. Rustan, G. Hege Thoresen, Eili Tranheim Kase
Summary: This study aimed to develop and characterize a skeletal muscle 3D cell model called myospheres for studying molecular mechanisms of energy metabolism. The myospheres showed structural and molecular characteristics of mature skeletal muscle and exhibited significant metabolic differences compared to the 2D myotubes model. These findings highlight the importance of considering model differences when studying energy metabolism and metabolic disorders in skeletal muscle.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Environmental Sciences
Ruijia Zhang, Baowei Chen, Li Lin, Hui Zhang, Tiangang Luan
Summary: The study found that perfluorooctanoic acid (PFOA) primarily affects glucose metabolism by altering the tricarboxylic acid (TCA) cycle rather than glycolysis, leading to impaired mitochondrial respiratory function. Metabolic flux analysis (MFA) is a promising tool for investigating the toxicity mechanisms of contaminants associated with glucose metabolism.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Nutrition & Dietetics
Naoya Yanagi, Tomotaka Koike, Kentaro Kamiya, Nobuaki Hamazaki, Kohei Nozaki, Takafumi Ichikawa, Atsuhiko Matsunaga, Masayuki Kuroiwa, Masayasu Arai
Summary: Sarcopenia diagnosed in the ICU, based on low skeletal muscle mass and function, is significantly associated with 1-year mortality, indicating that muscle mass and function assessments at the bedside could help identify higher-risk patients in the ICU.
Article
Biochemistry & Molecular Biology
Yanhong Li, Jie Wang, Mauricio A. Elzo, Mingchuan Gan, Tao Tang, Jiahao Shao, Tianfu Lai, Yuan Ma, Xianbo Jia, Songjia Lai
Summary: Through experiments, it was found that in rabbits induced to be obese by a high-fat diet, there were significant differences in the expression of certain miRNAs and core genes, with the miRNAs being able to regulate transcription factors that impact lipid metabolism. These results contribute to a better understanding of the molecular mechanisms of skeletal muscle metabolism in rabbits.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Nutrition & Dietetics
Saori Onishi, Akira Fukuda, Masahiro Matsui, Kosuke Ushiro, Tomohiro Nishikawa, Akira Asai, Soo Ki Kim, Hiroki Nishikawa
Summary: This study examined body composition using bioimpedance analysis in patients with metabolic dysfunction-associated fatty liver disease (MAFLD). The results showed a strong correlation between fat-free mass index (FF index) and muscle mass index. BMI and fat mass index were significant factors linked to FF index.
Article
Biochemistry & Molecular Biology
Luz Marina Sanchez-Mendoza, Carlos Perez-Sanchez, Sandra Rodriguez-Lopez, Chary Lopez-Pedrera, Miguel Calvo-Rubio, Rafael de Cabo, Maria I. Buron, Jose A. Gonzalez-Reyes, Jose M. Villalba
Summary: The study investigates the effect of sex on metabolic adaptations induced by overexpression of CYB5R3 and the modulation of key markers related to mitochondrial function in skeletal muscle. It was found that CYB5R3 overexpression leads to enhanced mitochondrial biogenesis and function, as well as increased mitochondrial abundance in skeletal muscle. These beneficial actions are predominantly observed in females, with differences in NADH levels and the abundance of cytochrome c and DRP-1. The results also show ultrastructural changes in transgenic females, including an increase in the number and size of mitochondria.
FREE RADICAL BIOLOGY AND MEDICINE
(2023)
Article
Multidisciplinary Sciences
Ettore Vanni, Karina Lindner, Anne-Claude Gavin, Christophe Montessuit
Summary: The formation of lipid droplets (LD) is associated with glucose uptake, while the inhibition of diacylglycerol acyltransferases (DGAT) affects glucose uptake under metabolic stress stimulation. This regulation is mediated through the diacylglycerol/triacylglycerol ratio.
SCIENTIFIC REPORTS
(2023)
Article
Endocrinology & Metabolism
Abhinav Achreja, Tao Yu, Anjali Mittal, Srinadh Choppara, Olamide Animasahun, Minal Nenwani, Fulei Wuchu, Noah Meurs, Aradhana Mohan, Jin Heon Jeon, Itisam Sarangi, Anusha Jayaraman, Sarah Owen, Reva Kulkarni, Michele Cusato, Frank Weinberg, Hye Kyong Kweon, Chitra Subramanian, Max S. Wicha, Sofia D. Merajver, Sunitha Nagrath, Kathleen R. Cho, Analisa DiFeo, Xiongbin Lu, Deepak Nagrath
Summary: In this study, the researchers develop a framework called CLIM to identify collateral lethal genes using metabolic fluxes, and they use it to discover MTHFD2 as a collateral lethal gene in UQCR11-deleted ovarian tumors. They demonstrate that MTHFD2 plays a non-canonical oxidative role by providing mitochondrial NAD(+) and show the regulation of systemic metabolic activity by the paralogue metabolic pathway. The researchers also confirm the UQCR11-MTHFD2 collateral lethality in vivo, with complete remission of UQCR11-deleted ovarian tumors upon MTHFD2 inhibition.
Article
Physics, Applied
Shinji Bono, Riku Takahashi, Satoshi Konishi
Summary: Vertical contact control can manipulate the concentration of chemicals in microdroplets and achieve three-dimensional microfluidic systems. Experimental results show that the volume difference of microdroplets, AV, increases proportionally to the fifth power of the size of microdroplets, R, which can be predicted quantitatively with material parameters such as density and surface tension.
PHYSICAL REVIEW APPLIED
(2021)
Review
Biochemistry & Molecular Biology
Lauren Jun, Megan Robinson, Thangiah Geetha, Tom L. L. Broderick, Jeganathan Ramesh Babu
Summary: Skeletal muscle atrophy is a prevalent issue in numerous pathological conditions and has significant implications for the health of society, particularly older adults. The detrimental effects of muscle atrophy include functional disability, increased fracture risk, decreased metabolic rate, and reduced bone density. This review article focuses on the prevalence and mechanisms of skeletal muscle atrophy, with particular emphasis on high-fat, high-sugar diets, obesity, and diabetes, as well as other conditions such as sarcopenia, Alzheimer's disease, cancer cachexia, and heart failure.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Huiting Wei, Di Cui
Summary: Skeletal muscle insulin resistance disrupts glucose homeostasis and is associated with pyroptosis. While pyroptosis can eliminate pathogenic factors, excessive activation can aggravate inflammatory responses. Further research is needed to understand the relationship between pyroptosis and skeletal muscle insulin resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Takuya Kobayashi, Nagomi Kurebayashi, Takashi Murayama
Summary: The Ryanodine receptor (RyR) is a crucial Ca2+ release channel in skeletal and cardiac muscles, regulated by various intracellular factors. Changes in conditions such as exercise, fatigue, and disease states can alter RyR channel activity, indicating its potential role as a sensor for intracellular environment changes in muscles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Adele Kerjouan, Cyril Boyault, Christiane Oddou, Edwige Hiriart-Bryant, Alexei Grichine, Alexandra Kraut, Mylene Pezet, Martial Balland, Eva Faurobert, Isabelle Bonnet, Yohann Coute, Bertrand Fourcade, Corinne Albiges-Rizo, Olivier Destaing
Summary: Researchers developed an optogenetic probe to control SRC signaling, demonstrating its ability to generate different acto-adhesive structures by manipulating SRC kinase activity. Through proteomic and network analyses, they revealed the characteristics of distinct downstream signaling pathways regulated by optoSRC dynamics.
JOURNAL OF CELL SCIENCE
(2021)
Article
Multidisciplinary Sciences
Ljudmila Klepinina, Aleksandr Klepinin, Laura Truu, Vladimir Chekulayev, Heiki Vija, Kaisa Kuus, Indrek Teino, Martin Pook, Toivo Maimets, Tuuli Kaambre
Summary: The study demonstrates that modulating cell metabolism through NaBT can be an effective strategy for treating colorectal cancer (CRC), with significant impacts on the energy metabolism and differentiation state of CRC cells.
Article
Biochemistry & Molecular Biology
Song Zhang, Satsuki Yamada, Sungjo Park, Aleksandr Klepinin, Tuuli Kaambre, Andre Terzic, Petras Dzeja
Summary: The study revealed that AK2 plays a crucial role in early embryonic development and its loss in adults can lead to sudden heart failure, which is eventually recovered with an adaptive response. The compensatory upregulation of related enzymes and genes highlights the innate plasticity of the AK2-integrated bioenergetics system in maintaining homeostasis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Cell Biology
Julien Aureille, Mylene Pezet, Lydia Pernet, Jacques Mazzega, Alexei Grichine, Christophe Guilluy, Monika Elzbieta Dolega
Summary: Deciphering how individual cells process and respond to signals from the environment is a central challenge in developmental biology and disease understanding. Recent genomic studies have provided information about population heterogeneity, but spatial heterogeneity remains poorly linked with mechanical and biochemical cues. A new method based on the use of photo convertible proteins has been developed to study genetic alterations and spatial distribution of cells in response to environmental signals.
MOLECULAR BIOLOGY OF THE CELL
(2021)
Review
Oncology
Leenu Reinsalu, Marju Puurand, Vladimir Chekulayev, Sten Miller, Igor Shevchuk, Kersti Tepp, Egle Rebane-Klemm, Natalja Timohhina, Anton Terasmaa, Tuuli Kaambre
Summary: Metabolic plasticity is the ability of cells to adjust metabolism to environmental changes. Cancer cells exhibit increased metabolic plasticity, allowing them to have a survival advantage and higher proliferative capacity. This enhanced plasticity is characterized by changes in adenine nucleotide transport, impaired regulation of VDAC and reprogrammed energy transfer circuits like AK and CK pathways.
FRONTIERS IN ONCOLOGY
(2021)
Article
Cell Biology
Slawomir Blonski, Julien Aureille, Sara Badawi, Damian Zaremba, Lydia Pernet, Alexei Grichine, Sandrine Fraboulet, Piotr M. Korczyk, Pierre Recho, Christophe Guilluy, Monika E. Dolega
Summary: Cell shape dynamics during development are tightly regulated and coordinated with cell fate determination. The interplay between biochemical and mechanical signals triggers complex tissue formation through coordinated cell shape changes. Signaling gradients associated with folding control spatially distinct biochemical responses within the monolayer.
DEVELOPMENTAL CELL
(2021)
Article
Oncology
Aleksandr Klepinin, Sten Miller, Indrek Reile, Marju Puurand, Egle Rebane-Klemm, Ljudmila Klepinina, Heiki Vija, Song Zhang, Andre Terzic, Petras Dzeja, Tuuli Kaambre
Summary: The dynamics of ATP phosphoryl turnover and metabolic flux through phosphotransfer pathways in cancer cells are not well understood. In this study, using O-18 phosphometabolite tagging technology, the authors investigated the phosphotransfer dynamics in three breast cancer cell lines and found a decreased turnover of γ- and β-ATP in cancer cells compared to control. They also observed reduced fluxes through HK, CK, and mitochondrial substrate shuttle in aggressive cancer cells. The downregulation of MTCK1A was linked to the decreased CK metabolic flux. However, overexpression of HK2, AK2, and AK6 isoforms in cell compartments could promote aggressive breast cancer growth.
FRONTIERS IN ONCOLOGY
(2022)
Article
Chemistry, Applied
Simon Pascal, San-Hui Chi, Alexei Grichine, Veronique Martel-Frachet, Joseph W. Perry, Olivier Maury, Chantal Andraud
Summary: This study reports the synthesis and optical properties investigation of amino-heptamethines, providing new insights into the two-photon absorption of this type of chromophores and exploring their potential for bio-imaging of living cells.
Article
Chemistry, Inorganic & Nuclear
Ji-Hyung Choi, Guillaume Fremy, Thibault Charnay, Nour Fayad, Jacques Pecaut, Sule Erbek, Niko Hildebrandt, Veronique Martel-Frachet, Alexei Grichine, Olivier Seneque
Summary: In this article, a novel microscopy imaging probe based on lanthanide complexes is described. The probe exhibits desirable luminescence properties and cell-penetrating ability for 2P imaging of live cells. By conjugating the lanthanide complexes with cell-penetrating peptides, successful synthesis of the conjugates was achieved and their application in various microscopy imaging techniques was evaluated. The results demonstrate that the probe has proper internalization and cytosolic delivery in cells, while maintaining its original luminescence properties. These findings provide new insights for the development of functional probes for live cell microscopy imaging.
INORGANIC CHEMISTRY
(2022)
Article
Materials Science, Multidisciplinary
Silvia Mizzoni, Silvia Ruggieri, Annika Sickinger, Francois Riobe, Laure Guy, Margaux Roux, Guillaume Micouin, Akos Banyasz, Olivier Maury, Bruno Baguenard, Amina Bensalah-Ledoux, Stephan Guy, Alexei Grichine, Xuan-Nhi Nguyen, Andrea Cimarelli, Martina Sanadar, Andrea Melchior, Fabio Piccinelli
Summary: In this study, two pairs of cationic enantiomeric complexes were synthesized and characterized. They showed good sensitization of Sm(iii) and Eu(iii) luminescence and exhibited high CPL activity. In cell experiments, these complexes showed promise as nanoscale bioprobes.
JOURNAL OF MATERIALS CHEMISTRY C
(2023)
Article
Biochemistry & Molecular Biology
Kersti Tepp, Jekaterina Aid-Vanakova, Marju Puurand, Natalja Timohhina, Leenu Reinsalu, Karin Tein, Mario Plaas, Igor Shevchuk, Anton Terasmaa, Tuuli Kaambre
Summary: This study aimed to investigate the role of Wfs1 deficiency on muscle bioenergetics. The results revealed that Wfs1 gene mutation led to a decrease in oxygen consumption rate in muscles, with substrate-dependent alterations. Additionally, it was found that the respiration rate of muscles significantly decreased during the development of WS, although other changes were relatively minor.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2022)
Correction
Chemistry, Analytical
Karl Kristjan Kaup, Lauri Toom, Laura Truu, Sten Miller, Marju Puurand, Kersti Tepp, Tuuli Kaambre, Indrek Reile
Article
Chemistry, Analytical
Karl Kristjan Kaup, Lauri Toom, Laura Truu, Sten Miller, Marju Puurand, Kersti Tepp, Tuuli Kaambre, Indrek Reile
Summary: In this study, a combination of established pure shift NMR and artifact suppression techniques was used to record P-31 pure shift NMR spectra without extending measurement times or causing line broadening. This approach successfully resolved overlapping multiplets of homonuclear coupled phosphorus nuclei, demonstrating its potential for phosphometabolomics analysis.
Article
Biochemistry & Molecular Biology
Brandon P. Russell, David J. Vinyard
Summary: The Mn4CaO5 oxygen-evolving complex in Photosystem II is crucial for water oxidation. D1 residue R334 participates in proton release and interacts with PsbO. A D1-R334G mutant destabilizes the OEC but stabilizes the S2 intermediate.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Alexander A. Bulychev, Tatiana S. Strelets
Summary: Excitable cells of higher plants and characean algae respond to stressful stimuli by generating action potentials that influence chlorophyll fluorescence and photosynthesis for an extended period of time. While plant leaves exhibit a reversible depression in the efficiency of photosystem II reaction after an individual action potential, characean algae show long-lasting oscillations of photosystem II reaction efficiency after firing an action potential. This study investigates the possible mechanisms behind these oscillations and suggests that they are a result of metabolic rearrangements in chloroplasts and the cyclosis cessation-recovery cycle induced by calcium influx during action potentials. The findings also indicate that fluidic communications between different cell regions play a role in these oscillations, and the inhibition of oscillations occurs when these communications are restricted or eliminated.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Dmitry Zlenko, Elena A. Protasova, Georgy Tsoraev, Nikolai N. Sluchanko, Dmitry A. Cherepanov, Thomas Friedrich, Baosheng Ge, Song Qin, Eugene G. Maksimov, Andrew B. Rubin
Summary: The conformation of chromophores in isolated phycobiliproteins is heterogeneous, but not in the entire phycobilisome (PBS). Under low-energy excitation, there is no significant uphill energy transfer from the core to the peripheral rods of the PBS, while transfer from the terminal emitters to bulk allophycocyanin chromophores is highly probable.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Makio Yokono, Chiyo Noda, Jun Minagawa
Summary: This paper investigates the energy transfer between Photosystem II and Photosystem I in Arabidopsis thaliana, and finds that the fast spillover is reversibly regulated depending on pH.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
