Review
Chemistry, Multidisciplinary
Xuechen Li, Xing Liang, Junling Yin, Weiying Lin
Summary: Autophagy is a common degradation process in cells that plays crucial roles in biological activities, but its abnormalities are closely related to various diseases. Researchers have explored organic fluorescent probes in recent years for real-time monitoring of autophagy process, discussing design strategies, applications, and limitations of these probes.
CHEMICAL SOCIETY REVIEWS
(2021)
Article
Cell Biology
Andrey Bogorodskiy, Ivan Okhrimenko, Ivan Maslov, Nina Maliar, Dmitrii Burkatovskii, Florian von Ameln, Alexey Schulga, Philipp Jakobs, Joachim Altschmied, Judith Haendeler, Alexandros Katranidis, Ivan Sorokin, Alexey Mishin, Valentin Gordeliy, Georg Buldt, Wolfgang Voos, Thomas Gensch, Valentin Borshchevskiy
Summary: The study shows that a new microinjection method can be used to directly observe protein transport in living cells. The researchers found that releasing nascent polypeptides from translating ribosomes with puromycin increased the import rate of proteins into mitochondria.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Chemistry, Analytical
Mingtai Sun, Tian Wang, Xin Yang, Huan Yu, Suhua Wang, Dejian Huang
Summary: A new fluorescent probe BV-1 based on BODIPY group was synthesized and demonstrated to detect viscosity in living cells with high selectivity and sensitivity. The probe showed significant enhancement in fluorescence with redshift in emission wavelength under high viscosity conditions, providing efficient ways for the development of viscosity probes and monitoring of intracellular viscosity properties and functions.
Article
Chemistry, Multidisciplinary
Jingting Zhan, Chen Geng, Xinya Hao, Wenhui Song, Weiying Lin
Summary: The novel NIF fluorescent probe ZM-V was designed with interior imidazole and benzopyrene moieties as rotators, showing significant red-fluorescence enhancement with a 111-fold fluorescence intensity change from water to glycerol. ZM-V exhibited a large Stokes shift of 166 nm and was able to target mitochondria with a high Pearson's correlation coefficient of 0.94, detecting changes in mitochondrial viscosity. It was also successfully used to monitor viscosity variations in zebrafish and mice, showcasing its superior properties.
NEW JOURNAL OF CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Jun Xiang Ong, Hai Van Le, Violet Eng Yee Lee, Wee Han Ang
Summary: Mitochondria have been identified as important targets for cisplatin in cancer treatment. In addition to cisplatin, anticancer Pt complexes with similar structures have been developed to target mitochondria. The researchers developed a mitochondria-targeted fluorescent probe to monitor Pt accumulation in mitochondria in real time, and identified two distinct pathways through which Pt complexes could be delivered to mitochondria.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Analytical
Keqin Yang, Li Hou, Zhifang Li, Tianran Lin, Jianniao Tian, Shulin Zhao
Summary: A ratiometric fluorescent nanoprobe for detecting peroxynitrite was successfully constructed, showing selective accumulation in mitochondria and linear detection in the range of 0-6.0 µM. This nanoprobe enables ratiometric fluorescence imaging of endogenous peroxynitrite in cell mitochondria.
Review
Genetics & Heredity
Eduardo Ruiz-Pesini, Julio Montoya, David Pacheu-Grau
Summary: In human mitochondria, mtDNA encodes a small fraction of proteins while the majority are encoded by the nuclear genome and imported into mitochondria through various import machineries. Proper coordination of these molecular pathways is crucial for mitochondrial homeostasis. Studying these pathways is important for understanding and expanding the genetic landscape of mitochondrial diseases.
Article
Spectroscopy
Mengwen Yi, Nan Zhang, Xiangjun Liu, Jing Liu, Xiangru Zhang, Yongbiao Wei, Dihua Shangguan
Summary: In this study, a hemicyanine-based off-on fluorescent probe, CO-H1, was developed for monitoring endogenous mitochondrial CO levels in living cells. CO-H1 exhibited significant fluorescence enhancement and red shift of absorption upon reaction with CO in the presence of PdCl2. The probe demonstrated low cytotoxicity, high sensitivity (detection limit of 0.048 μM), and good selectivity for CO. CO-H1 mainly targeted the mitochondria in living cells and successfully imaged exogenous/endogenous mitochondrial CO, indicating its potential for further studying the biological functions of mitochondrial CO in living cells.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Xueling Liu, Yali Wang, Guangshuai Zhou, Wenzhou Zhang
Summary: This study developed a fluorescent probe mito-ACS for imaging mitochondrial hypochlorite in living cells. The probe exhibited fast response, low detection limit, good water solubility, and selective localization to mitochondria. It showed great potential in fluorescent imaging of biological systems.
Article
Chemistry, Organic
Sheng Wang, Yuanyuan Li, Youfang Gan, Rui Wang
Summary: In this study, we utilized azidotrimethylsilane/Selectfluor via single electron transfer radical reaction to introduce azido group selectively into various prenylated molecules. After optimizing the conditions, we achieved fluorescent labeling of nucleic acids with prenyl modification in vitro using this established protocol, followed by click reaction with DBCO-Cy5.
TETRAHEDRON LETTERS
(2022)
Article
Chemistry, Applied
Yadan Huo, Yuan Ji, Dingguo Chen, Shouzhi Pu
Summary: The study presents the design of a mitochondrial-targeted probe HDCl for fast and sensitive detection of HClO in living cells, which can be successfully applied for mitochondrial imaging.
Article
Chemistry, Analytical
Fulan Xie, Rui Zhou, Chi Jian, Lizhu Zhang, Yonghui He
Summary: As a bioactive species with strong oxidation capacity, peroxynitrite (ONOO-) plays a crucial role in regulating various pathophysiological processes. The overproduction of ONOO- is closely associated with physiological diseases such as liver injury and pulmonary fibrosis. In this study, two borate-based fluorescent probes 3a and 3b were synthesized for the detection of ONOO-. The probes exhibited high selectivity and sensitivity with detection limits of 79.46 nM and 32.12 nM, respectively. Furthermore, the probes showed low cytotoxicity and successfully detected endogenous and exogenous ONOO-. These probes provide an efficient method for studying the physiological and pathological role of ONOO- in complex biological systems and related diseases.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Multidisciplinary
Le Zhang, Yi-Fan Zhang, Ying-Feng Han
Summary: A fluorescent probe for OCl- detection in mitochondria was developed, showing high selectivity, ultra-fast response, and capability for cellular imaging.
MATERIALS CHEMISTRY FRONTIERS
(2022)
Article
Spectroscopy
Yuting Du, Caixia Pan, Chunjuan Cao
Summary: In this research, a mitochondrial-target probe Mito-NPH was designed and synthesized, which exhibited specific response to HSO3-/ SO32- and could sense endogenous SO2 in mitochondria.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Yang Shu, Yunfan Liu, Yiru Gao, Jiaying Li
Summary: In this study, a new fluorescence probe ANET was developed for specific detection and imaging of MAO-A in mitochondria. ANET showed specific recognition and reaction with MAO-A in mitochondria, resulting in the release of product EHBT. The fluorescence signal of EHBT was activated at pH 8.0 and showed a linear relationship with MAO-A concentration.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Multidisciplinary
Katherine Villa, Hanna Sopha, Jaroslav Zelenka, Martin Motola, Lukas Dekanovsky, Darya Chylii Beketova, Jan M. Macak, Tomas Ruml, Martin Pumera
Summary: The study introduces a novel hybrid enzyme/photocatalytic microrobot that can swim in urea and respond to visible light, effectively removing bacterial biofilms. The results demonstrate a synergistic effect between self-propulsion provided by the enzyme and photocatalytic activity induced under light stimuli, showcasing potential applications in microbiology and biomedicine.
Article
Biochemistry & Molecular Biology
Pavla Pruchova, Klara Gotvaldova, Katarina Smolkova, Lukas Alan, Blanka Holendova, Jan Tauber, Alexander Galkin, Petr Jezek, Martin Jaburek
Summary: This study reveals the antioxidant and anti-inflammatory roles of iPLA(2)gamma in the brain. It demonstrates that iPLA(2)gamma is activated by tert-butylhydroperoxide and releases free fatty acids, which leads to an increase in respiratory rate and a decrease in mitochondrial superoxide production. It also shows that iPLA(2)gamma plays a key role in the remodeling of cardiolipins in the brain.
Article
Chemistry, Multidisciplinary
Carmen C. Mayorga-Martinez, Jaroslav Zelenka, Karel Klima, Paula Mayorga-Burrezo, Lan Hoang, Tomas Ruml, Martin Pumera
Summary: Efficient eradication of dental biofilm on titanium dental implants can be achieved using swarming magnetic microrobots, demonstrating their potential in precision medicine.
Article
Chemistry, Inorganic & Nuclear
Kaplan Kirakci, Michaela Kubanova, Tomas Pribyl, Michaela Rumlova, Jaroslav Zelenka, Tomas Ruml, Kamil Lang
Summary: Singlet oxygen photosensitizers that target cell membranes have been developed for enhanced photodynamic therapy. A singlet oxygen photosensitizing complex, made of a molybdenum-iodine nanocluster stabilized by triazolate apical ligands, was prepared using copper-free click chemistry. This complex targeted cell membranes and exhibited a significant phototoxic effect against HeLa cells. The nature of the apical ligands plays a dominant role in the biological properties of the nanocluster. The complex also showed antibacterial effects on pathogenic strains.
INORGANIC CHEMISTRY
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Lukas Alan, Aleksandra Markovic, Katerina Tauchmannova, Miroslava Simakova, Petr Mlejnek, Vilma Kaplanova, Petr Pecina, Alena Pecinova, Jan Silhavy, Josef Houstek, Michal Pravenec, Tomas Mracek
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2022)
Article
Biochemical Research Methods
Marketa Kulisova, Olga Matatkova, Tomas Branyik, Jaroslav Zelenka, Lucie Drabova, Irena Jarosova Kolouchova
Summary: Microscopic filamentous fungi are adaptable microorganisms that can form biofilms on various surfaces. This study aimed to find a reliable method for measuring the biofilm growth of selected fungi strains. Multiple methods were tested, and crystal violet staining was found to be suitable for determining total biofilm biomass, while the MTT assay was suitable for determining its metabolic activity. Fluorescent stains successfully dyed the hyphae of microscopic fungi.
JOURNAL OF MICROBIOLOGICAL METHODS
(2023)
Article
Chemistry, Multidisciplinary
Carmen C. C. Mayorga-Martinez, Marketa Castoralova, Jaroslav Zelenka, Tomas Ruml, Martin Pumera
Summary: In this study, a method based on magnetic microrobots was developed for rapid isolation and identification of Staphylococcus aureus bacteria in milk. The method does not produce harmful compounds for the milk microbiota and can be efficiently applied in food production.
Article
Chemistry, Multidisciplinary
Carmen C. Mayorga-Martinez, Jaroslav Zelenka, Karel Klima, Michaela Kubanova, Tomas Ruml, Martin Pumera
Summary: Modern micro/nanorobots have versatile capabilities for biomedical and environmental tasks. Magnetic microrobots are particularly promising for biomedical applications, as they can be fully controlled by a rotating magnetic field without the use of toxic fuels. These microrobots can also form swarms to perform tasks at a larger scale.
ADVANCED MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Petr Jezek, Martin Jaburek, Blanka Holendova, Hana Engstova, Andrea Dlaskova
Summary: The mitochondrial reticulum network in cells consists of parallel lamellar cristae formed by invaginated inner mitochondrial membrane. The non-invaginated part, the inner boundary membrane, forms a cylindrical sandwich with the outer mitochondrial membrane. The dimensions, shape, and junctions of cristae have characteristic patterns for different metabolic regimes, physiological and pathological situations.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Article
Biochemistry & Molecular Biology
Ippei Kawano, Bazila Bazila, Petr Jezek, Andrea Dlaskova
Summary: The architecture of the mitochondrial network and cristae critically impact cell differentiation and identity. Recent studies have shown that manipulating mitochondrial dynamics and cristae shape affects T cell phenotype, macrophage polarization, and other cellular processes. Further investigation of the molecular mechanisms involved could lead to improved therapeutic manipulation of cell viability, differentiation, proliferation, and identity.
ANTIOXIDANTS & REDOX SIGNALING
(2023)
Article
Multidisciplinary Sciences
Vojtech Pavluch, Tomas Spacek, Hana Engstova, Andrea Dlaskova, Petr Jezek
Summary: Using 3D-double-color direct stochastic optical reconstruction microscopy (dSTORM) and a new method of Delaunay segmentation of 3D data, we identified the presence of two or more mitochondrial DNA molecules in nucleoids in HeLa cells, indicating a low level of nucleoid division. This finding is important for understanding the organization and distribution of mitochondrial DNA.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Inorganic & Nuclear
Regis Guegan, Xiaoxue Cheng, Xiang Huang, Zuzana Nemeckova, Michaela Kubanova, Jaroslav Zelenka, Tomas Ruml, Fabien Grasset, Yoshiyuki Sugahara, Kamil Lang, Kaplan Kirakci
Summary: The photoinactivation of pathogenic bacteria using materials generating reactive oxygen species upon exposure to visible light can overcome the challenge of multidrug-resistant microbial pathogens. A nanocomposite material consisting of graphene oxide sheets adorned with nanoaggregates of octahedral molybdenum cluster complexes has been developed. The electrostatic interaction and close cohesion between the components in aqueous medium contributed to the potent antibacterial effect against Staphylococcus aureus upon illumination with blue light. The photoinduced electron transfer from the nanoaggregates to the graphene oxide sheets triggers the generation of reactive oxygen species, making graphene oxide and light-harvesting octahedral molybdenum cluster complexes promising for effective antibacterial strategies.
INORGANIC CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Petr Jezek
Summary: This article theoretically derives the mutual influence between mitochondria and the cytosol, predicting possible conditions for mitochondrial signals to the cytosol and plasma membrane. The involvement of peroxiredoxin 3 in mitochondrial-to-cytosol redox signaling is discussed, as well as the activation of matrix MnSOD in a specific case. The use of redox-sensitive probes and their impact on quantifying H2O2 fluxes is also considered.
Article
Chemistry, Inorganic & Nuclear
Regis Guegan, Xiaoxue Cheng, Xiang Huang, Zuzana Nemeckova, Michaela Kubanova, Jaroslav Zelenka, Tomas Ruml, Fabien Grasset, Yoshiyuki Sugahara, Kamil Lang, Kaplan Kirakci
Summary: The emergence of multidrug-resistant microbial pathogens poses a significant threat to effective antibiotic therapy. Photoinactivation using materials generating reactive oxygen species upon exposure to visible light can overcome this challenge by targeting vital components of living cells, reducing the likelihood of resistance development.
INORGANIC CHEMISTRY
(2023)
Article
Materials Science, Biomaterials
Martina Koncosova, Michaela Rumlova, Romana Mikyskova, Milan Reinis, Jaroslav Zelenka, Tomas Ruml, Kaplan Kirakci, Kamil Lang
Summary: X-ray-induced photodynamic therapy, utilizing radiosensitizing nanoparticles to produce reactive oxygen species, offers a promising modality for the treatment of various malignancies. It overcomes the limitations of conventional photodynamic therapy and reduces the required dose for cancer eradication within radiotherapy treatment.
JOURNAL OF MATERIALS CHEMISTRY B
(2022)
Article
Biochemistry & Molecular Biology
Brandon P. Russell, David J. Vinyard
Summary: The Mn4CaO5 oxygen-evolving complex in Photosystem II is crucial for water oxidation. D1 residue R334 participates in proton release and interacts with PsbO. A D1-R334G mutant destabilizes the OEC but stabilizes the S2 intermediate.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Alexander A. Bulychev, Tatiana S. Strelets
Summary: Excitable cells of higher plants and characean algae respond to stressful stimuli by generating action potentials that influence chlorophyll fluorescence and photosynthesis for an extended period of time. While plant leaves exhibit a reversible depression in the efficiency of photosystem II reaction after an individual action potential, characean algae show long-lasting oscillations of photosystem II reaction efficiency after firing an action potential. This study investigates the possible mechanisms behind these oscillations and suggests that they are a result of metabolic rearrangements in chloroplasts and the cyclosis cessation-recovery cycle induced by calcium influx during action potentials. The findings also indicate that fluidic communications between different cell regions play a role in these oscillations, and the inhibition of oscillations occurs when these communications are restricted or eliminated.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Dmitry Zlenko, Elena A. Protasova, Georgy Tsoraev, Nikolai N. Sluchanko, Dmitry A. Cherepanov, Thomas Friedrich, Baosheng Ge, Song Qin, Eugene G. Maksimov, Andrew B. Rubin
Summary: The conformation of chromophores in isolated phycobiliproteins is heterogeneous, but not in the entire phycobilisome (PBS). Under low-energy excitation, there is no significant uphill energy transfer from the core to the peripheral rods of the PBS, while transfer from the terminal emitters to bulk allophycocyanin chromophores is highly probable.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Makio Yokono, Chiyo Noda, Jun Minagawa
Summary: This paper investigates the energy transfer between Photosystem II and Photosystem I in Arabidopsis thaliana, and finds that the fast spillover is reversibly regulated depending on pH.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
