Review
Oncology
Leenu Reinsalu, Marju Puurand, Vladimir Chekulayev, Sten Miller, Igor Shevchuk, Kersti Tepp, Egle Rebane-Klemm, Natalja Timohhina, Anton Terasmaa, Tuuli Kaambre
Summary: Metabolic plasticity is the ability of cells to adjust metabolism to environmental changes. Cancer cells exhibit increased metabolic plasticity, allowing them to have a survival advantage and higher proliferative capacity. This enhanced plasticity is characterized by changes in adenine nucleotide transport, impaired regulation of VDAC and reprogrammed energy transfer circuits like AK and CK pathways.
FRONTIERS IN ONCOLOGY
(2021)
Article
Biology
Jakob Michaelsen, Angela Fago, Amanda Bundgaard
Summary: The study found that under high temperature conditions, respiratory rates and complex I activity of rainbow trout cardiac mitochondria decrease, while phosphorylation is impaired. In contrast, complex II activity remains stable at temperatures above a certain point.
JOURNAL OF EXPERIMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Morteza Esmaeili, Riyas Vettukattil
Summary: Magnetic resonance spectroscopy (MRS) is a non-invasive technique that allows for the study of cardiac metabolism in vivo. However, technical limitations currently restrict its utility. Further improvements are needed for clinical application.
Review
Physiology
Craig A. Lygate
Summary: In order to fully understand gene function, it is necessary to study the effects in an intact organism. The creation of the first knockout mouse revolutionized the field of integrative physiology, with focus on potential problems in interpretation of in vivo cardiac phenotypes. The study of creatine kinase energy system in intact organisms highlights the impact of genetic background, sex, and age, and the challenges in interpreting results from knockout models.
FRONTIERS IN PHYSIOLOGY
(2021)
Review
Microbiology
Martin A. Prusinkiewicz, Joe S. Mymryk
Summary: Recent research has shown that DNA tumor viruses can influence host cell metabolism by interacting with MYC, p53, or the pRb/E2F complex. Interestingly, the same virus can activate or inhibit host cell regulators, depending on the viral oncoprotein interacting with the regulatory protein. Understanding how DNA tumor viruses regulate metabolism through viral oncoproteins could lead to new antiviral therapeutic strategies.
Article
Environmental Sciences
Ruijia Zhang, Baowei Chen, Li Lin, Hui Zhang, Tiangang Luan
Summary: The study found that perfluorooctanoic acid (PFOA) primarily affects glucose metabolism by altering the tricarboxylic acid (TCA) cycle rather than glycolysis, leading to impaired mitochondrial respiratory function. Metabolic flux analysis (MFA) is a promising tool for investigating the toxicity mechanisms of contaminants associated with glucose metabolism.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biochemical Research Methods
Krista Yang, Mary T. Doan, Linsey Stiles, Ajit S. Divakaruni
Summary: This paper introduces protocols for measuring CPT-1-mediated respiration and evaluating the efficacy and specificity of interventions targeting CPT-1. The strengths of the approach include ease of use, speed, and broad analysis, while the drawbacks include loss of physiological regulation and indirect assessment of CPT-1 enzymatic activity.
Article
Cardiac & Cardiovascular Systems
Jelena Branovets, Niina Karro, Karina Barsunova, Martin Laasmaa, Craig A. Lygate, Marko Vendelin, Rikke Birkedal
Summary: In mice with creatine deficiency, the hexokinase and adenylate kinase did not change, but in AGAT KO mice, increased expression and activity of hexokinase I were found, which could affect mitochondrial regulation and reactive oxygen species production.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Emilie Dubois-Deruy, Yara El Masri, Annie Turkieh, Philippe Amouyel, Florence Pinet, Jean-Sebastien Annicotte
Summary: Lysine acetylation is a conserved mechanism that affects various biological processes, and its dysregulation is associated with cardiac dysfunction. This review summarizes the regulation of cardiac acetylation and its roles in both physiological and pathological conditions, highlighting its importance in cardiovascular diseases and suggesting KATs and KDACs as potential therapeutic targets for heart failure.
Article
Neurosciences
Liam F. Fitzgerald, Miles F. Bartlett, Rajakumar Nagarajan, Ericber Jimenez Francisco, Frank C. Sup, Jane A. Kent
Summary: Muscle metabolic economy (ME) is similar in young and older adults during some isometric contractions, but age-related differences exist during dynamic contractions. Older adults have lower ME during dynamic contractions, despite similar muscle oxidative capacity and ATP flux compared to younger adults. This suggests a dissociation between cross-bridge activity and ATP utilization with age.
JOURNAL OF PHYSIOLOGY-LONDON
(2021)
Article
Biochemistry & Molecular Biology
Barbara Elsnicova, Daniela Hornikova, Veronika Tibenska, David Kolar, Tereza Tlapakova, Benjamin Schmid, Markus Mallek, Britta Eggers, Ursula Schloetzer-Schrehardt, Viktoriya Peeva, Carolin Berwanger, Bettina Eberhard, Hacer Durmus, Dorothea Schultheis, Christian Holtzhausen, Karin Schork, Katrin Marcus, Jens Jordan, Thomas Luecke, Peter F. M. van der Ven, Rolf Schroeder, Christoph S. Clemen, Jitka M. Zurmanova
Summary: Desmin mutations cause cardiomyopathies and negatively impact mitochondria. The deficiency of desmin results in impaired oxidative phosphorylation and fatty and amino acid metabolism in cardiac mitochondria. Increased glucose utilization and upregulation of fetal creatine kinase are compensatory mechanisms to maintain myocardial energy supply.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Matthias Mietsch, Rabea Hinkel
Summary: With the prevalence of cardiovascular diseases, there is an urgent need for new treatment strategies. While stem cell based approaches have shown promise in improving cardiac functions, recent research has highlighted the potential importance of mitochondria in these treatments.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Physiology
Sehwan Jang, Xavier R. Chapa-Dubocq, Silvia Fossati, Sabzali Javadov
Summary: The study compared the quantification of mitochondrial CRC in isolated cell mitochondria and permeabilized cells, demonstrating higher CRC in saponin-permeabilized cells. This indicates that quantifying mitochondrial CRC in cultured cells permeabilized by saponin has more advantages compared to isolated mitochondria.
FRONTIERS IN PHYSIOLOGY
(2021)
Review
Cell Biology
Michael P. Wiggs, Anna G. Beaudry, Michelle L. Law
Summary: Cancer cachexia is a syndrome that causes progressive weight loss and muscle wasting in advanced cancer patients, leading to impaired quality of life and increased mortality. This review focuses on the cardiac changes occurring in cachexia and discusses the underlying physiological and molecular mechanisms that contribute to cachexia-induced cardiomyopathy. Understanding the specific effects of cachexia on the heart may lead to the identification of unique therapeutic targets and improve clinical management for cancer cachexia patients.
Review
Cardiac & Cardiovascular Systems
E. Douglas Lewandowski
Summary: Cardiac function relies on efficient metabolic support, which is a dynamic process that adjusts to the demands of the heart. Flux through metabolic pathways provides energy and signaling molecules to meet these demands. Inability of metabolic flux to keep pace with cardiomyocyte demands leads to dysfunction and contributes to cardiac disease. Studying metabolic mechanisms as therapeutic targets and detecting metabolic flux in the heart will provide important insights into cardiac function and disease.
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
(2023)
Review
Physiology
Marina Makrecka-Kuka, Edgars Liepinsh, Andrew J. Murray, Helene Lemieux, Maija Dambrova, Kersti Tepp, Marju Puurand, Tuuli Kaambre, Woo H. Han, Paul de Goede, Katie A. O'Brien, Belma Turan, Erkan Tuncay, Yusuf Olgar, Anabela P. Rolo, Carlos M. Palmeira, Neoma T. Boardman, Rob C. I. Wust, Terje S. Larsen
Review
Pharmacology & Pharmacy
Marisol Ruiz-Meana, Kerstin Boengler, David Garcia-Dorado, Derek J. Hausenloy, Tuuli Kaambre, Georgios Kararigas, Cinzia Perrino, Rainer Schulz, Kirsti Ytrehus
BRITISH JOURNAL OF PHARMACOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Kersti Tepp, Marju Puurand, Natalja Timohhina, Jekaterina Aid-Vanakova, Indrek Reile, Igor Shevchuk, Vladimir Chekulayev, Margus Eimre, Nadada Peet, Lumme Kadaja, Kalju Paju, Tuuli Kaambre
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2020)
Editorial Material
Oncology
Sara Rodriguez-Enriquez, Tuuli Kaambre, Rafael Moreno-Sanchez
FRONTIERS IN ONCOLOGY
(2020)
Article
Multidisciplinary Sciences
Ljudmila Klepinina, Aleksandr Klepinin, Laura Truu, Vladimir Chekulayev, Heiki Vija, Kaisa Kuus, Indrek Teino, Martin Pook, Toivo Maimets, Tuuli Kaambre
Summary: The study demonstrates that modulating cell metabolism through NaBT can be an effective strategy for treating colorectal cancer (CRC), with significant impacts on the energy metabolism and differentiation state of CRC cells.
Article
Biochemistry & Molecular Biology
Song Zhang, Satsuki Yamada, Sungjo Park, Aleksandr Klepinin, Tuuli Kaambre, Andre Terzic, Petras Dzeja
Summary: The study revealed that AK2 plays a crucial role in early embryonic development and its loss in adults can lead to sudden heart failure, which is eventually recovered with an adaptive response. The compensatory upregulation of related enzymes and genes highlights the innate plasticity of the AK2-integrated bioenergetics system in maintaining homeostasis.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Oncology
Leenu Reinsalu, Marju Puurand, Vladimir Chekulayev, Sten Miller, Igor Shevchuk, Kersti Tepp, Egle Rebane-Klemm, Natalja Timohhina, Anton Terasmaa, Tuuli Kaambre
Summary: Metabolic plasticity is the ability of cells to adjust metabolism to environmental changes. Cancer cells exhibit increased metabolic plasticity, allowing them to have a survival advantage and higher proliferative capacity. This enhanced plasticity is characterized by changes in adenine nucleotide transport, impaired regulation of VDAC and reprogrammed energy transfer circuits like AK and CK pathways.
FRONTIERS IN ONCOLOGY
(2021)
Article
Oncology
Aleksandr Klepinin, Sten Miller, Indrek Reile, Marju Puurand, Egle Rebane-Klemm, Ljudmila Klepinina, Heiki Vija, Song Zhang, Andre Terzic, Petras Dzeja, Tuuli Kaambre
Summary: The dynamics of ATP phosphoryl turnover and metabolic flux through phosphotransfer pathways in cancer cells are not well understood. In this study, using O-18 phosphometabolite tagging technology, the authors investigated the phosphotransfer dynamics in three breast cancer cell lines and found a decreased turnover of γ- and β-ATP in cancer cells compared to control. They also observed reduced fluxes through HK, CK, and mitochondrial substrate shuttle in aggressive cancer cells. The downregulation of MTCK1A was linked to the decreased CK metabolic flux. However, overexpression of HK2, AK2, and AK6 isoforms in cell compartments could promote aggressive breast cancer growth.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Kersti Tepp, Jekaterina Aid-Vanakova, Marju Puurand, Natalja Timohhina, Leenu Reinsalu, Karin Tein, Mario Plaas, Igor Shevchuk, Anton Terasmaa, Tuuli Kaambre
Summary: This study aimed to investigate the role of Wfs1 deficiency on muscle bioenergetics. The results revealed that Wfs1 gene mutation led to a decrease in oxygen consumption rate in muscles, with substrate-dependent alterations. Additionally, it was found that the respiration rate of muscles significantly decreased during the development of WS, although other changes were relatively minor.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2022)
Correction
Chemistry, Analytical
Karl Kristjan Kaup, Lauri Toom, Laura Truu, Sten Miller, Marju Puurand, Kersti Tepp, Tuuli Kaambre, Indrek Reile
Article
Chemistry, Analytical
Karl Kristjan Kaup, Lauri Toom, Laura Truu, Sten Miller, Marju Puurand, Kersti Tepp, Tuuli Kaambre, Indrek Reile
Summary: In this study, a combination of established pure shift NMR and artifact suppression techniques was used to record P-31 pure shift NMR spectra without extending measurement times or causing line broadening. This approach successfully resolved overlapping multiplets of homonuclear coupled phosphorus nuclei, demonstrating its potential for phosphometabolomics analysis.
Article
Biochemistry & Molecular Biology
Brandon P. Russell, David J. Vinyard
Summary: The Mn4CaO5 oxygen-evolving complex in Photosystem II is crucial for water oxidation. D1 residue R334 participates in proton release and interacts with PsbO. A D1-R334G mutant destabilizes the OEC but stabilizes the S2 intermediate.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Alexander A. Bulychev, Tatiana S. Strelets
Summary: Excitable cells of higher plants and characean algae respond to stressful stimuli by generating action potentials that influence chlorophyll fluorescence and photosynthesis for an extended period of time. While plant leaves exhibit a reversible depression in the efficiency of photosystem II reaction after an individual action potential, characean algae show long-lasting oscillations of photosystem II reaction efficiency after firing an action potential. This study investigates the possible mechanisms behind these oscillations and suggests that they are a result of metabolic rearrangements in chloroplasts and the cyclosis cessation-recovery cycle induced by calcium influx during action potentials. The findings also indicate that fluidic communications between different cell regions play a role in these oscillations, and the inhibition of oscillations occurs when these communications are restricted or eliminated.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Dmitry Zlenko, Elena A. Protasova, Georgy Tsoraev, Nikolai N. Sluchanko, Dmitry A. Cherepanov, Thomas Friedrich, Baosheng Ge, Song Qin, Eugene G. Maksimov, Andrew B. Rubin
Summary: The conformation of chromophores in isolated phycobiliproteins is heterogeneous, but not in the entire phycobilisome (PBS). Under low-energy excitation, there is no significant uphill energy transfer from the core to the peripheral rods of the PBS, while transfer from the terminal emitters to bulk allophycocyanin chromophores is highly probable.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)
Article
Biochemistry & Molecular Biology
Makio Yokono, Chiyo Noda, Jun Minagawa
Summary: This paper investigates the energy transfer between Photosystem II and Photosystem I in Arabidopsis thaliana, and finds that the fast spillover is reversibly regulated depending on pH.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2024)