Journal
BIOCHEMISTRY-MOSCOW
Volume 77, Issue 13, Pages 1553-1574Publisher
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S0006297912130111
Keywords
lanthanide fluorescence assay; time-resolved fluorescence resonance energy transfer (TR-FRET); lanthanide-binding peptides (LBPs); fluorescent proteins (FPs); multiphoton excitation; bioimaging; molecular sensors
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Funding
- Russian Foundation for Basic Research [11-02-01532-a]
- Russian Federation Ministry of Education and Science [16.512.11.2137]
- Presidium of the Russian Academy of Sciences
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This review describes key directions in the development of different probes based on complex compounds of lanthanides for in vitro and in vivo researches. The role of microsecond fluorescence of lanthanides for overcoming problems of background fluorescence is considered. The basic classes of synthetic and genetically encoded complex compounds of lanthanides are summarized. Main principles of designing lanthanide-based molecular sensors, including FRET sensors based on lanthanides and colored fluorescent proteins are described. Their applications in bioanalytical research and cellular bioimaging are described. The main principles of cellular bioimaging using lanthanides are formulated, questions of their delivery into cells are considered, and the problem of their potential toxicity for living organisms is discussed. A technique using multiphoton excitation of lanthanides is described.
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