Article
Agricultural Engineering
Rachapudi Sreeharsha, G. Sai Tejaswini, S. Venkata Mohan
Summary: This study focuses on lipid and volatile fatty acid (VFA) production through dual-stage fermentation of spentwash in a biorefinery framework with Trichosporon cutaneum (Tc) and Yarrowia lipolytica (Yl). The results showed that Tc and Yl accumulated dry biomass of 2.9 and 2.5 g/L respectively in acidhydrolysed spentwash (AHSW) and produced total VFA of 16 and 5.5 g/L respectively. The lipid yields and titres were higher in Tc/AHSW compared to Yl, indicating the effectiveness of Tc in spentwash bioremediation. Furthermore, the analysis of fatty acid composition revealed the presence of higher oleic acid, which is ideal for biodiesel production.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biochemical Research Methods
Lin Chen, Wei Yan, Xiujuan Qian, Minjiao Chen, Xiaoyu Zhang, Fengxue Xin, Wenming Zhang, Min Jiang, Katrin Ochsenreither
Summary: This study successfully improved the acetate utilization capability of Yarrowia lipolytica by overexpressing key enzymes, leading to increased microbial lipid production efficiency from acetate.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Biology
Jingbo Ma, Yang Gu, Peng Xu
Summary: This study reports a systematic approach to improve the biosynthesis of the cannabinoid precursor olivetolic acid in yeast, leading to a significant increase in the production of the acid. The findings provide a baseline for engineering cannabinoids biosynthesis in oleaginous yeast species.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Patrycja Szczepanska, Magdalena Rychlicka, Pawel Moroz, Tomasz Janek, Anna Gliszczynska, Zbigniew Lazar
Summary: Phospholipids are a class of lipids with diverse biological functions and are commonly used in lipid replacement therapy. This study found that Yarrowia lipolytica is a promising producer of phospholipids, especially using waste substrates.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Veronika Urbanikova, Young-Kyoung Park, Daniela Krajciova, Mehdi Tachekort, Milan Certik, Ioana Grigoras, Roman Holic, Jean-Marc Nicaud, Peter Gajdos
Summary: In this study, the oleaginous yeast Yarrowia lipolytica was used as a host for the production of Punicic acid (PuA). By supplementing the medium with pomegranate seed oil, lipids containing PuA esters were accumulated up to 31.2% in Y. lipolytica. Strategies involving the expression of the bifunctional fatty acid conjugase/desaturase gene from Punica granatum (PgFADX) allowed for de novo production of PuA in Y. lipolytica. The optimized promoter for PgFADX expression resulted in a PuA production of 36.6 mg/L. These findings highlight the potential of Y. lipolytica as a promising host for PuA production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Naghmeh Poorinmohammad, Jing Fu, Bob Wabeke, Eduard J. Kerkhoven
Summary: This study investigated the molecular mechanism underlying lipid accumulation in Yarrowia lipolytica through proteomic analysis. Various cellular functions and biological mechanisms related to lipid accumulation were identified, and genetic engineering of selected targets was used to validate their effects on lipid content, providing new strategies to optimize lipid yield.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Yongshuo Ma, Nian Liu, Per Greisen, Jingbo Li, Kangjian Qiao, Sanwen Huang, Gregory Stephanopoulos
Summary: This study reports the removal of lycopene substrate inhibition in Yarrowia lipolytica by two different strategies, resulting in high carotenoid productivity. The findings provide effective approaches for efficient synthesis of natural products.
NATURE COMMUNICATIONS
(2022)
Article
Biotechnology & Applied Microbiology
Bruno Roswag Machado, Susan Hartwig Duarte, Lucielen Oliveira Santos
Summary: The study aimed to investigate the cultivation conditions for Yarrowia lipolytica to produce extracellular lipase and the influence of magnetic fields (MF) on lipase production and catalytic conditions. The optimal culture conditions for lipase production were 10 g L-1 glucose, 15 g L-1 olive oil, and 2 g L-1 Triton X-100. The application of MF from 72 to 144 hours significantly increased lipase activity by 287.5% compared to the control culture, without affecting the optimal temperature of lipase (37 °C), but altering the optimal pH to 8.0.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Qiang Yan, Tyler B. Jacobson, Zhou Ye, Yoel R. Cortes-Pena, Sarang S. Bhagwat, Susan Hubbard, William T. Cordell, Rebecca E. Oleniczak, Francesca V. Gambacorta, Julio Rivera Vazquez, Eric V. Shusta, Daniel Amador-Noguez, Jeremy S. Guest, Brian F. Pfleger
Summary: Plants produce valuable oleochemical molecules, but meeting the global demand is challenging due to limited land availability and environmental impact. Metabolic engineering offers a solution by transferring oleochemical metabolism to controllable hosts and accessing molecules from non-agricultural plants. This study focuses on rewiring metabolism in Yarrowia lipolytica to synthesize acetyl-diacyl-sn-glycerol (acTAG), which has potential applications as low-grade fuels, lubricants, and emulsifiers. The research establishes acTAG production at g/L scale, explores the impact of lipid bodies, and conducts a techno-economic analysis to determine economic feasibility.
METABOLIC ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Jaroslav Hambalko, Peter Gajdos, Jean-Marc Nicaud, Rodrigo Ledesma-Amaro, Michal Tupec, Iva Pichova, Milan Certik
Summary: Fatty alcohols are aliphatic primary alcohols with four or more carbon atoms that have potential industrial and biological applications. They are produced in nature as part of pheromone mixtures in insect species and may also be used as substitutes for commercial insecticides in agriculture. Biotechnological production of fatty alcohol components using engineered microbial strains shows promise for sustainable ecological practices.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Ewelina Celinska
Summary: Yarrowia lipolytica is a popular yeast species used in biotechnological production processes. This review summarizes the current state-of-art of understanding Y. lipolytica's molecular response to stress and adaptation, including the deregulation of genes, proteins, molecules, and biological processes. It highlights the specificities and universalities of Y. lipolytica's cellular response to different threats.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Svetlana Kamzolova, Julia N. Lunina, Vladimir A. Samoilenko, Igor G. Morgunov
Summary: Yarrowia lipolytica yeast is capable of synthesizing citric acid, and the concentration of ammonium sulfate significantly affects its growth and citric acid production.
Article
Biochemistry & Molecular Biology
Maria Gorczyca, Jan Kazmierczak, Patrick Fickers, Ewelina Celinska
Summary: Overproduction of recombinant secretory proteins triggers changes in producer cell physiology, and exposure to suboptimal growth conditions may increase this response. Studying the effects of different environmental stresses on protein synthesis and host cell physiology can improve understanding of fundamental biological processes and provide clues for optimizing bioprocesses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
Vijaydev Ganesan, Lummy Monteiro, Dheeraj Pedada, Anthony Stohr, Mark Blenner
Summary: Yarrowia lipolytica is an industrial host with a high fatty acid flux. In this study, we developed a rapid multiplexed strain engineering tool using base editors for efficient gene editing. The base editors were found to be less toxic than CRISPR-Cas9 for multiplexed gene editing. By optimizing the editing conditions and using a co-selection approach, we achieved high-efficiency multiplexed gene editing.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Maria Gorczyca, Jean-Marc Nicaud, Ewelina Celinska
Summary: Resistance to environmental stress and synthesis of recombinant proteins (r-Prots) are complex biological traits that rely on multiple genes. Modifying the operation of transcription factors (TFs) associated with these traits can be a strategy to engineer them. This study examined the implications of five selected TFs in stress resistance and r-Prot synthesis in Yarrowia lipolytica. Over-expression or deletion of the TFs in a host strain resulted in significant changes in growth and r-Prot yields under specific conditions. Mathematical modeling assisted in understanding the contribution of individual TFs and identifying new functions of the studied TFs.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
A. G. Rogov, T. A. Trendeleva, D. A. Aliverdieva, R. A. Zvyagilskaya
BIOCHEMISTRY-MOSCOW
(2016)
Review
Biochemistry & Molecular Biology
T. A. Trendeleva, R. A. Zvyagilskaya
BIOCHEMISTRY-MOSCOW
(2018)
Article
Biochemistry & Molecular Biology
A. G. Rogov, T. N. Goleva, T. A. Trendeleva, A. P. Ovchenkova, D. A. Aliverdieva, R. A. Zvyagilskaya
BIOCHEMISTRY-MOSCOW
(2018)
Review
Biochemistry & Molecular Biology
B. V. Chernyak, Y. N. Antonenko, E. R. Galimov, L. V. Domnina, V. B. Dugina, R. A. Zvyagilskaya, O. Yu. Ivanova, D. S. Izyumov, K. G. Lyamzaev, A. V. Pustovidko, T. I. Rokitskaya, A. G. Rogov, I. I. Severina, R. A. Simonyan, M. V. Skulachev, V. N. Tashlitsky, E. V. Titova, T. A. Trendeleva, G. S. Shagieva
BIOCHEMISTRY-MOSCOW
(2012)
Article
Biochemistry & Molecular Biology
T. A. Trendeleva, A. G. Rogov, D. A. Cherepanov, E. I. Sukhanova, T. M. Il'yasova, I. I. Severina, R. A. Zvyagilskaya
BIOCHEMISTRY-MOSCOW
(2012)
Review
Biochemistry & Molecular Biology
T. A. Trendeleva, D. A. Aliverdieva, R. A. Zvyagilskaya
BIOCHEMISTRY-MOSCOW
(2014)
Article
Biochemistry & Molecular Biology
Ljudmila S. Khailova, Denis N. Silachev, Tatyana I. Rokitskaya, Armine V. Avetisyan, Konstantin G. Lyamsaev, Irma I. Severina, Tatyana M. Il'yasova, Mikhail V. Gulyaev, Vera I. Dedukhova, Tatyana A. Trendeleva, Egor Y. Plotnikov, Renata A. Zvyagilskaya, Boris V. Chernyak, Dmitry B. Zorov, Yuri N. Antonenko, Vladimir P. Skulachev
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2014)
Review
Biochemistry & Molecular Biology
Inna I. Severina, Fedor F. Severin, Galina A. Korshunova, Natalya V. Sumbatyan, Tatyana M. Ilyasova, Ruben A. Simonyan, Anton G. Rogov, Tatyana A. Trendeleva, Renata A. Zvyagilskaya, Vera B. Dugina, Lidia V. Domnina, Elena K. Fetisova, Konstantin G. Lyamzaev, Mikhail Yu Vyssokikh, Boris V. Chernyak, Maxim V. Skulachev, Vladimir P. Skulachev, Viktor A. Sadovnichii
Article
Biophysics
Mariya V. Kovaleva, Evgeniya I. Sukhanova, Tatyana A. Trendeleva, Marina V. Zyl'kova, Ludmila A. Ural'skaya, Kristina M. Popova, Nils-Erik L. Saris, Renata A. Zvyagilskaya
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES
(2009)
Article
Cell Biology
Antonina V. Pustovidko, Tatiana I. Rokitskaya, Inna I. Severina, Ruben A. Simonyan, Tatiana A. Trendeleva, Konstantin G. Lyamzaev, Yuri N. Antonenko, Anton G. Rogov, Renata A. Zvyagilskaya, Vladimir P. Skulachev, Boris V. Chernyak
Article
Cell Biology
Tatiana A. Trendeleva, Evgenia I. Sukhanova, Anton G. Rogov, Renata A. Zvyagilskaya, Inna I. Seveina, Tatiana M. Ilyasova, Dmitry A. Cherepanov, Vladimir P. Skulachev
Article
Cell Biology
T. N. Goleva, A. G. Rogov, G. A. Korshunova, T. A. Trendeleva, D. V. Mamaev, D. A. Aliverdieva, R. A. Zvyagilskaya
Article
Cell Biology
Anton G. Rogov, Tatyana N. Goleva, Evgeniya Sukhanova, Khoren K. Epremyan, Tatiana A. Trendeleva, Alexandra P. Ovchenkova, Dinara A. Aliverdieva, Renata A. Zvyagilskaya
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2020)
