Article
Chemistry, Physical
JunWoo Kim, Tu C. Nguyen-Phan, Alastair T. Gardiner, Richard J. Cogdell, Gregory D. Scholes, Minhaeng Cho
Summary: The study utilizes a dual-laser 2DES technique to investigate the electronic energy transfer process in bacterial light-harvesting complexes. Through principal component analysis, it is found that the energy transfer is modulated by a low-frequency vibrational mode of the exciton donor.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2021)
Article
Chemistry, Physical
Mykyta Onizhuk, Siddhartha Sohoni, Giulia Galli, Gregory S. Engel
Summary: This study explores the interplay between the arrangement of light-harvesting antennae and the efficiency of exciton transport in purple bacterial photosynthesis. The results show that arrangements with aggregated LH1s have a higher efficiency than arrangements with randomly distributed LH1s in a wide range of physiological light fluences. This effect is suggested to be an adaptive strategy toward solar light fluence across different purple bacterial species.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2021)
Article
Biotechnology & Applied Microbiology
Tong Shi, Lu Zhang, Mindong Liang, Weishan Wang, Kefeng Wang, Yue Jiang, Jing Liu, Xinwei He, Zhiheng Yang, Haihong Chen, Chuan Li, Dongyuan Lv, Liming Zhou, Biqin Chen, Dan Li, Li-Xin Zhang, Gao-Yi Tan
Summary: In this study, several native promoters from Rhodobacter sphaeroides JDW-710 were characterized, with two showing significantly higher activity than the commonly used tac promoter. A synthetic promoter library was also created, leading to successful modulation of the Q10 synthetic pathway in JDW-710 and increased production yields.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2021)
Article
Environmental Sciences
Hao-Tian Mao, Lun-Xing Chen, Meng-Ying Zhang, Qiu-Yun Shi, Hong Xu, Da-Yan Zhang, Zhong-Wei Zhang, Ming Yuan, Shu Yuan, Huai -Yu Zhang, Yan-Qiu Su, Yang -Er Chen
Summary: In this study, R. sphaeroides SC01 and melatonin were employed to remove hexavalent chromium from wastewater. The results showed that exogenous melatonin could alleviate oxidative damage, enhance the absorption capacity of cell membranes, and improve the reduction efficiency of hexavalent chromium. Melatonin application led to significant upregulation of ascorbic acid, glutathione, and cysteine, and promoted the bioprecipitation of CrPO4·6H2O. These findings highlight the important role of melatonin in the reduction and uptake of hexavalent chromium, making it a promising strategy for the remediation of contaminated wastewater by photosynthetic bacteria.
ENVIRONMENTAL POLLUTION
(2023)
Article
Energy & Fuels
Yu Rim Lee, Soo Youn Lee, Jiye Lee, Hui Su Kim, Jin-Suk Lee, Won-Heong Lee, Sangmin Lee
Summary: Global warming is currently accelerating due to an increase in greenhouse gas emissions. Microbial electrosynthesis (MES) using electroactive autotrophic microorganisms has been reported as a method to reduce carbon dioxide. However, the application of MES is limited, and the molecular mechanisms are unknown. This study found that increasing antioxidant activities to reduce reactive oxygen species (ROS) levels is important for enhancing cell growth and production of CO2-converting substances in MES.
Article
Physics, Multidisciplinary
Andrea Mattioni, Felipe Caycedo-Soler, Susana F. Huelga, Martin B. Plenio
Summary: The study explores the impact of quantum phenomena in molecular aggregates on energy transfer mechanisms, demonstrating that quantum properties of small configurations can be used to regulate energy transfer on larger scales. Through numerical simulations and an exactly solvable model, it is shown that this regulation is crucial for achieving classical propagation over macroscopic distances.
Article
Chemistry, Physical
Lilit Hakobyan, Lilit Gabrielyan, Syuzanna Blbulyan, Armen Trchounian
Summary: Selecting the right source for biohydrogen production is crucial for bacterial metabolism. While pure organic substrates can generate H-2 quickly, they come at a higher cost, making the use of industrial wastes a more economical option for energy generation. The growth medium containing optimal amount of wastes could potentially be a successful alternative to expensive media for high H-2 yield in Rhodobacter sphaeroides during photofermentation.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2021)
Article
Agriculture, Multidisciplinary
Yuling Qu, Anping Su, Ying Li, Yonghong Meng, Zhi Chen
Summary: By deleting the photosynthetic gene cluster repressor and overexpressing the activator, the lycopene production of Rhodobacter sphaeroides was significantly increased, especially under high oxygen conditions.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Yujun Xiao, Yi Zheng, Yong Zhou, Chaofan Yu, Ting-E Ye
Summary: Coenzyme Q(10) is crucial in biology and medicine. This study investigated the conditions for increasing CoQ(10) production by analyzing the metabolic flux in Rhodobacter sphaeroides. The results showed that adjusting the pH with calcium carbonate led to a higher yield of CoQ(10) compared to using aqueous ammonia. Additionally, G6P and chorismic acid were identified as key nodes in the metabolic network. This study provides a theoretical basis for future gene modifications related to the CoQ(10) synthesis pathway.
MICROBIAL CELL FACTORIES
(2023)
Article
Chemistry, Physical
Bo Hyun Choi, Sung Hui Kim, Pyung Cheon Lee
Summary: The study investigated the relationship between the structure and CDB-introducing capability of Phytoene desaturase CrtI, identifying three key amino acids that significantly determined carotenoid profiles. The mutated amino acids were found to be located in the membrane-binding domain of CrtI, influencing the FAD binding region and subsequently affecting the desaturation level in phytoene. These findings contribute to a better understanding of the function and regulation of CrtI in carotenoid biosynthesis.
Article
Agricultural Engineering
Ferdinand Schmid, Julia Novion Ducassou, Yohann Coute, Johannes Gescher
Summary: This study identified Rhodobacter sphaeroides as a potential cathodic production strain for photoautotrophic processes. By introducing a plasmid vector and exposure to antibiotic pressure, significant improvement in cathodic biofilm formation and electrochemical properties were achieved. Quantitative proteomic analysis and optical coherence tomography measurements revealed key factors and kinetics of biofilm growth on the cathodic surface. Switching media to N2-limited conditions resulted in increased accumulation of cathodic poly(3-hydroxybutyrate) (PHB), indicating the potential of R. sphaeroides for photoautotrophic PHB production in future industrial applications.
BIORESOURCE TECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Daniel Jun, Sylvester Zhang, Adrian Jan Grzedowski, Amita Mahey, J. Thomas Beatty, Dan Bizzotto
Summary: The use of biomacromolecules in clean alternative energies is a developing field, with potential applications in biosensors and biophotovoltaic devices. Research has shown that the assembly of bacterial photosynthetic reaction center (RC) on electrodes and its local environment significantly impact photocurrent generation, with implications for electron transfer of other biomacromolecules in sensor and photovoltaic applications.
Article
Chemistry, Applied
Yaqin Wang, Lingjia Fan, Jintao Huang, Jingjing Liang, Xingnan Wang, Yichen Ren, Hongcai Li, Tianli Yue, Zhenpeng Gao
Summary: This study found that pumpkin juice fermented by Rhodobacter sphaeroides contains high levels of coenzyme Q10, exhibits strong antioxidant activity, and can regulate the gut microbiota of mice.
Article
Chemistry, Physical
Yoshitaka Saga, Yuji Otsuka, Aiko Tanaka, Yuto Masaoka, Tsubasa Hidaka, Yutaka Nagasawa
Summary: Excitation energy transfer in light-harvesting proteins is determined by the pigment compositions and their organizations. Changing the pigment compositions helps to better understand the EET mechanisms. The study shows that EET from AcChl a to B850 instead of B800 is rationalized by little spectral overlap of AcChl a with B800 BChl a and the pigment geometry in the protein.
JOURNAL OF PHYSICAL CHEMISTRY B
(2021)
Article
Microbiology
Haoyu Wang, Xiaoling Sha, Rui Li, Yijing Li, Himel Nahreen Khaleque, Yuxiu Zhang, Tsing Bohu, Zhihui Bai, Xuliang Zhuang
Summary: This study conducted a genome-wide comparative analysis of the strain EBL0706, confirming it as a strain of Luteovulum azotoformans. The genome of EBL0706 contains 4034 protein-coding genes, including a complete photosynthetic gene cluster.
Article
Biochemistry & Molecular Biology
Zhiping Zhao, Zongli Hu, Xin Nie, Lijing Cheng, Guolin Ding, Min Luo, Yu Pan, Yan Liang, Guoping Chen
PROTEIN AND PEPTIDE LETTERS
(2011)
Article
Environmental Sciences
Zhiping Zhao, Tao Peng, Jeong-Il Oh, Jens Glaeser, Lennart Weber, Qingfeng Li, Gabriele Klug
ENVIRONMENTAL MICROBIOLOGY REPORTS
(2019)
Review
Genetics & Heredity
Zhenhui Kang, Tong Qin, Zhiping Zhao
Article
Microbiology
Zhou Luo, Zhen Wang, Bangxu Wang, Yao Lu, Lixiu Yan, Zhiping Zhao, Ting Bai, Jiamin Zhang, Hanmei Li, Wei Wang, Jie Cheng
Summary: N-hydroxy-pipecolic acid (NHP), an important systemic acquired resistance signal molecule, has been successfully produced from biomass-derived lysine in Escherichia coli for the first time. This study lays the foundation for the development and utilization of renewable resources to produce NHP in microorganisms.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xin Nie, Hongfan Chen, Lu Xiang, Yulin Zhang, Dayu Liu, Zhiping Zhao
Summary: This study analyzed the metabolites and associated metabolic pathways of different ultra-long-term industrially fermented kohlrabies using metabolomics. The results provide a theoretical basis for targeted regulation of characteristic metabolite biosynthesis in Chinese fermented kohlrabi.
Article
Biotechnology & Applied Microbiology
Hongfan Chen, Xin Nie, Tao Peng, Lu Xiang, Dayu Liu, Huailiang Luo, Zhiping Zhao
Summary: This study investigated the impact of low-temperature and low-salt fermentation on the volatile flavor substances of Chinese kohlrabi. The results showed that low-temperature and low-salt fermentation significantly affected the physicochemical properties and volatile flavor profile of the Chinese kohlrabi. A total of 114 volatile flavor substances were identified, with significantly higher levels of certain compounds in the low-temperature and low-salt fermented Chinese kohlrabi compared to the traditional high-salt fermented Chinese kohlrabi. Multivariate statistical analysis revealed potential biomarkers for distinguishing the two types of Chinese kohlrabi. The findings support the development of green processing technology and new low-salt Chinese kohlrabi products.
FERMENTATION-BASEL
(2023)
Article
Food Science & Technology
Zhiping Zhao, Hongfan Chen, Yulin Zhang, Xin Nie, Lu Xiang, Tao Peng, Dayu Liu, Huailiang Luo, Aili Wang
Summary: A low-temperature and low-salt industrially fermented Chinese kohlrabi (LSCK) was developed, with a 70% decrease in salt usage compared to traditional high-salt fermented Chinese kohlrabi (HSCK). The physicochemical properties, metabolites, and overall flavors of LSCK fermented for 0, 45, and 90 days were analyzed. Significant changes were observed in acid content, protein content, and lactic acid bacteria cells. Differential metabolites were identified in LSCK fermented for different periods, with carbohydrates, sugar alcohols, amino acids, and their derivatives being the main ones. Aminoacyl-tRNA biosynthesis and glycine, serine, and threonine metabolism pathways were significantly correlated with the differential metabolites. The overall odors of LSCKs with different fermentation periods were also significantly different. This study provides valuable insights into the metabolite changes and important metabolic pathways in LSCK fermentation.
FRONTIERS IN SUSTAINABLE FOOD SYSTEMS
(2023)
Article
Food Science & Technology
Hongfan Chen, Yulin Zhang, Xinyi Wang, Xin Nie, Dayu Liu, Zhiping Zhao
Summary: This study explored the microbial diversity, volatile flavor substances, and their potential correlations in Chinese Qingcheng Mountain traditional bacon (CQTB). The analysis revealed 39 volatile flavor substances in CQTB, with significant differences between the inner and surface samples. High-throughput sequencing identified dominant bacterial and fungal phyla and genera. Correlation analysis showed a positive relationship between Apiotrichum and Lactobacillus and the formation of key phenol compounds. These findings provide valuable insights for the development of microbial starter culture and green processing of CQTB.
Article
Biochemistry & Molecular Biology
Zhenhui Kang, Tong Qin, Zhiping Zhao
ACTA BIOCHIMICA POLONICA
(2019)
Article
Biochemistry & Molecular Biology
Xin Nie, Li Zhang, Zongli Hu, Yi Liu, Zhiping Zhao
ACTA BIOCHIMICA POLONICA
(2015)
Article
Biochemistry & Molecular Biology
Zhi-ping Zhao, Ting-ting Liu, Li Zhang, Min Luo, Xin Nie, Zai-xin Li, Yu Pan
ACTA BIOCHIMICA POLONICA
(2014)