Article
Biochemistry & Molecular Biology
Francisco Esteves, Cristina M. M. Almeida, Sofia Silva, Ines Saldanha, Philippe Urban, Jose Rueff, Denis Pompon, Gilles Truan, Michel Kranendonk
Summary: A unique cytochrome P450 (CYP) oxidoreductase (CPR) is important for maintaining the activities of human microsomal CYPs. The conformation of the CPR protein influences electron transfers and complex formation with CYPs. Mutations in the hinge region and FD domain of CPR can modulate CYP activities and regiospecificity of reactions in a substrate-dependent manner.
Article
Chemistry, Physical
Lea R. Rapp, Sergio M. Marques, Bernd Nebel, Jiri Damborsky, Bernhard Hauer
Summary: The catalytic space of the P450 monooxygenase CYP153A(M.aq) was transformed from a fatty acid hydroxylase to a catalyst capable of omega-hydroxylation of dodecylamine. A rapid screening method called RAPP was established to measure saturation libraries directly from a 96-deepwell plate. Molecular modeling and dynamics simulations revealed significant effects of the mutations on the substrate tunnel architectures.
Article
Biochemistry & Molecular Biology
Tianxiang Xiao, Kai Lu
Summary: The common cutworm, Spodoptera litura, has developed resistance to commonly used insecticides. Two P450 enzymes, CYP6AE43 and CYP6AE48, play a crucial role in detoxifying pyrethroids and promoting resistance development in S. litura.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Review
Biochemistry & Molecular Biology
Carlos Gutierrez-Merino, Oscar H. Martinez-Costa, Maria Monsalve, Alejandro K. Samhan-Arias
Summary: Membrane cytochrome b(5) reductase is an oxidoreductase that reduces multiple acceptors localized in cellular membranes using NADH as an electron donor. The understanding of the electron transfer process of the reductase towards potential acceptors is still only partially understood. Computational analysis of protein structures provides a potential method to further investigate the roles of different domains within the reductase structure.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Zbigniew Dutkiewicz, Renata Mikstacka
Summary: In this study, the interactions between three selected polymethoxy-trans-stilbenes and the binding sites of CYP1 isozymes were investigated using molecular dynamics simulations. Significant structural changes were observed in two substrate recognition sites (SRS) of the CYP1 binding sites. The study also revealed the presence of water molecules in different positions in the structures, leading to the formation of various hydrogen bonds.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Biochemistry & Molecular Biology
F. Peter Guengerich
Summary: The paper introduces the concept of cytochrome b(5) as an auxiliary protein facilitating cytochrome P450 reactions, providing important insights for future research.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2022)
Article
Biochemistry & Molecular Biology
Lokeswari P. Tangella, Mahreen Arooj, Evelyne Deplazes, Elin S. Gray, Ricardo L. Mancera
Summary: In this study, an atomistic model of the full-length human ABCB5 transporter was generated and validated using molecular dynamics simulations, revealing at least three putative binding sites for chemotherapeutic drugs. These findings will serve as the basis for future in vitro studies to validate the nature of the identified substrate-binding sites in the full-length ABCB5 transporter.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Chemistry, Physical
P. Venkata Ramana, Y. Rama Krishna, K. Chandra Mouli
Summary: Cancer is the third leading cause of mortality worldwide and continues to pose a serious threat to human health. Research on anti-cancer drug molecules and their mechanisms of action is of great significance for cancer treatment.
JOURNAL OF MOLECULAR STRUCTURE
(2022)
Article
Electrochemistry
Celia M. Silveira, Patricia R. Rodrigues, Wissam Ghach, Sofia A. Pereira, Francisco Esteves, Michel Kranendonk, Mathieu Etienne, M. Gabriela Almeida
Summary: The study investigated the direct electrochemical response of membrane-bound human cytochrome P450 1A2 on pyrolytic graphite electrodes encapsulated in a sol-gel matrix. It showed that CYP1A2 is highly sensitive to O-2 and easily inactivated under aerobic conditions, but remains active when in the presence of its electron donor partner, cytochrome P450 oxidoreductase (CPR). This highlights the crucial role of CPR in stabilizing the immobilized CYP1A2 enzyme and preserving O-2 electrocatalysis in this electrochemical set-up.
Article
Environmental Sciences
Ritwick Bhattacharya, Arnab Chatterjee, Soumendranath Chatterjee, Nimai Chandra Saha
Summary: This study evaluated the interaction between Cyprinus carpio cytochrome P450 and surfactants through homology modelling and molecular docking experiments. The in silico analysis revealed the prevalent hydrophobic interactions and potential oxidative stress induced by the binding of cytochrome P450 with the surfactants. In vivo analysis showed that exposure to sublethal concentrations of surfactants adversely affected the oxidative stress and antioxidant enzymes in the liver of Cyprinus carpio.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Review
Chemistry, Medicinal
Yeo-Jung Kwon, Sangyun Shin, Young-Jin Chun
Summary: Human cytochrome P450 enzymes of the CYP1 family are critical in the metabolism of various substrates related to cancer pathogenesis and the pathogenesis of several human diseases, particularly in the activation and inactivation of therapeutic drugs. Their role in diseases and drug metabolism has attracted significant interest in the scientific community for the development of novel therapeutic strategies.
ARCHIVES OF PHARMACAL RESEARCH
(2021)
Review
Plant Sciences
Chang-Jun Liu
Summary: Cytochrome b(5) (CB5) is a small heme-binding protein that functions as an electron donor in oxidative reactions. In plants, the CB5 protein family has expanded isoform numbers and cellular functions compared to yeast and mammalian counterparts. Plant CB5 proteins are involved in fatty acid metabolism, specialized metabolite formation, and interact with non-catalytic proteins to regulate metabolic and cellular processes. The cellular and metabolic roles of plant CB5 proteins have received less attention compared to mammalian CB5 proteins. Understanding the biological functions of CB5 proteins in plants could have important implications for biotechnological applications.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Chemistry, Physical
Lea R. Rapp, Sergio M. Marques, Erna Zukic, Benjamin Rowlinson, Mahima Sharma, Gideon Grogan, Jiri Damborsky, Bernhard Hauer
Summary: The cytochrome P450 CYP153A(M.aq) enzyme from Marinobacter aquaeolei was engineered to hydroxylate octanoic acid with significantly improved catalytic efficiency and substrate binding. The modifications in tunnel structures and loop regions were key in stabilizing the enzyme-substrate complex and enhancing catalytic efficiency. Previous fatty acid anchor interactions were also found to boost the enzyme activity towards octanoic acid.
Article
Biochemistry & Molecular Biology
Maria Rita Gulotta, Serena Vittorio, Rosaria Gitto, Ugo Perricone, Laura De Luca
Summary: Investigating the molecular recognition pattern of the MUC1-CIN85 PPI provides new structural information for the design of potential anti-metastatic agents.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Engineering, Environmental
Yuhao Chu, Chaofan Zhang, Rupeng Wang, Xi Chen, Nanqi Ren, Shih-Hsin Ho
Summary: This study investigated the biotransformation of sulfamethoxazole by microalgae, particularly highlighting the role of CYP450 enzymes in the degradation process. The research elucidated the SMX biodegradation pathway involving CYP450 in microalgae, providing insights for using microalgae-mediated CYP450 to eliminate antibiotics.
Article
Chemistry, Organic
Alexandre Barrozo, Qinghua Liao, Mauricio Esguerra, Gael Marloie, Jan Florian, Nicholas H. Williams, Shina Caroline Lynn Kamerlin
ORGANIC & BIOMOLECULAR CHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Keriann Oertell, Jan Florian, Pouya Haratipour, Debbie C. Crans, Boris A. Kashemirov, Samuel H. Wilson, Charles E. McKenna, Myron F. Goodman
Article
Biochemistry & Molecular Biology
Radek Indra, Petr Pompach, Vaclav Martinek, Paulina Takacsova, Katarina Vavrova, Zbynek Heger, Vojtech Adam, Tomas Eckschlager, Katerina Kopeckova, Volker Manfred Arlt, Marie Stiborova
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Oncology
Jolantha Beyerle, Andreana N. Holowatyj, Mariam Haffa, Eva Frei, Biljana Gigic, Petra Schrotz-King, Juergen Boehm, Nina Habermann, Marie Stiborova, Dominique Scherer, Torsten Kolsch, Stephanie Skender, Nikolaus Becker, Esther Herpel, Martin Schneider, Alexis Ulrich, Peter Schirmacher, Jenny Chang-Claude, Hermann Brenner, Michael Hoffmeister, Ulrike Haug, Robert W. Owen, Cornelia M. Ulrich
CANCER EPIDEMIOLOGY BIOMARKERS & PREVENTION
(2020)
Article
Environmental Sciences
Radek Indra, Petr Pompach, Katarina Vavrova, Katefina Jaklova, Zbynek Heger, Vojtech Adam, Tomag Eckschlager, Katefina Kopeacova, Volker Manfred Arlt, Marie Stiborova
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Alzbeta Lengalova, Veronika Fojtikova-Proskova, Jakub Vavra, Vaclav Martinek, Martin Stranava, Toru Shimizu, Marketa Martinkova
JOURNAL OF INORGANIC BIOCHEMISTRY
(2019)
Article
Chemistry, Medicinal
Alena Dedikova, Frantisek Barta, Vaclav Martinek, Kevin Kotalik, Sarka Duskova, Jaroslav Mraz, Volker Manfred Arlt, Marie Stiborova, Petr Hodek
CHEMICAL RESEARCH IN TOXICOLOGY
(2020)
Article
Chemistry, Physical
Jazmin Calimano, Feifei Li, Jan Florian, Dalice M. Pinero-Cruz, Thomas R. Fielitz, Russell J. Holmes, Jacob W. Ciszek
JOURNAL OF PHYSICAL CHEMISTRY C
(2020)
Article
Biochemistry & Molecular Biology
Daniela Burska, Lukas Stiburek, Jana Krizova, Marie Vanisova, Vaclav Martinek, Jana Sladkova, Josef Zamecnik, Tomas Honzik, Jiri Zeman, Hana Hansikova, Marketa Tesarova
Summary: The study identified a novel homozygous missense mutation in the UQCRC2 gene, leading to severe encephalomyopathy, and highlighted the potential molecular mechanisms involved, including decreased CIII levels and insufficient accumulation of CI.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2021)
Article
Biochemistry & Molecular Biology
Frantisek Barta, Alena Dedikova, Michaela Bebova, Sarka Duskova, Jaroslav Mraz, Heinz H. Schmeiser, Volker M. Arlt, Petr Hodek, Marie Stiborova
Summary: The exposure to AAI/AAII mixture may enhance AA-DNA adduct formation in rat livers and kidneys, partially due to inhibition of AAI detoxification and induction of NQO1. Additionally, AAII may act as an inhibitor of AAI detoxification, leading to increased AAI residues in liver and kidney tissues and enhanced AAI DNA adduct formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Jakub Vavra, Artur Sergunin, Petr Jerabek, Toru Shimizu, Marketa Martinkova
Summary: This minireview discusses the mechanism of signal transduction in two heme-based oxygen sensors: the histidine kinase AfGcHK and the diguanylate cyclase YddV (EcDosC), both of which feature a heme-binding domain containing a globin fold resembling that of hemoglobin and myoglobin.
BIOLOGICAL CHEMISTRY
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Daniela Burska, Lukas Stiburek, Jana Krizova, Marie Vanisova, Vaclav Martinek, Tomas Honzik, Jiri Zeman, Hana Hansikova, Marketa Tesarova
EUROPEAN JOURNAL OF HUMAN GENETICS
(2022)
Review
Chemistry, Multidisciplinary
Toru Shimizu, Alzbeta Lengalova, Vaclav Martinek, Marketa Martinkova
CHEMICAL SOCIETY REVIEWS
(2019)
Meeting Abstract
Toxicology
R. Indra, K. Vavrova, P. Takacsova, P. Pompach, V. Martinek, Z. Heger, V. Adam, V. M. Arlt, M. Stiborova
TOXICOLOGY LETTERS
(2019)