Article
Biochemistry & Molecular Biology
Susumu Ohya, Junko Kajikuri, Hiroaki Kito, Miki Matsui
Summary: The up-regulation of CYP3A4 is responsible for the acquisition of doxorubicin resistance in cancer spheroid models. The inhibition of K(Ca)1.1 overcomes doxorubicin resistance by reducing the transcription level of CYP3A4 mainly through the Akt-Nrf2-CEBPB axis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Alexandro Landshammer, Adriano Bolondi, Helene Kretzmer, Christian Much, Rene Buschow, Alina Rose, Hua-Jun Wu, Sebastian D. Mackowiak, Bjoern Braendl, Pay Giesselmann, Rosaria Tornisiello, Krishna Mohan Parsi, Jack Huey, Thorsten Mielke, David Meierhofer, Rene Maehr, Denes Hnisz, Franziska Michor, John L. Rinn, Alexander Meissner
Summary: In this study, a previously unannotated lncRNA, T-REX17, was discovered upstream of the SOX17 gene. It was found to be induced following SOX17 activation and its expression was specifically restricted to early definitive endoderm. Loss of T-REX17 affected important functions independent of SOX17 and resulted in an aberrant endodermal transcriptome, signaling pathway deregulation, and defects in epithelial to mesenchymal transition. Consequently, cells lacking T-REX17 were unable to differentiate into more mature endodermal cell types. Overall, this study identified and characterized T-REX17 as a transiently expressed and essential non-coding regulator in early human endoderm differentiation.
Article
Cell Biology
Konstantin G. Lyamzaev, Alisa A. Panteleeva, Ruben A. Simonyan, Armine V. Avetisyan, Boris V. Chernyak
Summary: Ferroptosis induced by erastin and butionine sulfoximine can be prevented by the mitochondria-targeted antioxidants SkQ1 and MitoTEMPO. This prevention is associated with the inhibition of mitochondrial lipid peroxidation, which occurs before cell death. Methylene blue, a redox agent that inhibits reactive oxygen species (ROS) production in complex I of the mitochondrial electron transport chain, also inhibits ferroptosis and mitochondrial lipid peroxidation. We propose that ROS generated in complex I promote mitochondrial lipid peroxidation and ferroptosis.
Article
Green & Sustainable Science & Technology
Qinghua Fu, Jawad Abbas, Ghadahanfar Bin Alarif, Muhammad Safdar Sial, Talles Vianna Brugni, Nofal Adamwal
Summary: This study investigates whether green organizational culture (GOC) and corporate social responsibility (CSR) initiatives encourage eco-responsible behavior of workers (EERB) in their personal lives. The data from Turkey's public and commercial service and manufacturing firms are analyzed using structural equation modeling. The results show that employees of CSR and green-practicing businesses actively engage in eco-responsible practices in society. Additionally, businesses with a green culture are more likely to participate in CSR projects, and CSR serves as a mediator between GOC and EERB. This study suggests expanding the scope of social responsibility practices to individuals for achieving sustainable societal goals.
JOURNAL OF CLEANER PRODUCTION
(2023)
Article
Biochemistry & Molecular Biology
Shamchal Bakavayev, Shirel Argueti, Nachiyappan Venkatachalam, Galit Yehezkel, Alexandra Stavsky, Zeev Barak, Adrian Israelson, Stanislav Engel
Summary: The misfolding of SOD1 in ALS leads to the formation of a pathogenic protein, which can be targeted by the monoclonal antibody SE21 to potentially develop new treatment strategies. The reversible phase during misfolding provides a window of opportunity for pharmacological intervention to stabilize the structure of SOD1 and prevent disease progression. Additionally, diverse misfolding pathways of SOD1 can result in phenotypically similar species with impaired metal binding properties.
ACS CHEMICAL NEUROSCIENCE
(2021)
Article
Hematology
Tammy K. Truong, Rida A. Malik, Xintong Yao, James C. Fredenburgh, Alan R. Stafford, Hasam M. Madarati, Colin A. Kretz, Jeffrey Weitz
Summary: The histidine-rich glycoprotein (HRG) interacts with FXIIa and polyP through its histidine-rich region (HRR), thereby downregulating the contact pathway and contributing to the regulation of coagulation.
JOURNAL OF THROMBOSIS AND HAEMOSTASIS
(2022)
Article
Green & Sustainable Science & Technology
Emrah Kocak, Faik Bilgili, Umit Bulut, Sevda Kuskaya
Summary: The study shows that ethanol production increases corn prices, while oil prices and population exert pressure on corn prices. An increase in the real exchange rate has a decreasing effect on corn prices.
Article
Multidisciplinary Sciences
Emerson M. Carmona, Miguel Fernandez, Juan J. Alvear-Arias, Alan Neely, H. Peter Larsson, Osvaldo Alvarez, Jose Antonio Garate, Ramon Latorre, Carlos Gonzalez
Summary: The research reveals that the ApH-dependent gating in the Hv1 channel is achieved by modulating its voltage sensor. Analysis of the data indicates that around 60% of the free energy stored in the ApH is coupled to the activation of the Hv1 voltage sensor. Additionally, changes in the proton accessibility near the voltage sensor alter the landscape of free energy, affecting its activation process.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Business, Finance
Hongfeng Peng, Zhenqi Zhang, John W. Goodell, Mingsheng Li
Summary: This report points out that many mutual funds declare themselves as socially responsible investment (SRI) funds, but it is unclear whether this is just to attract capital or reflects genuine concern. The study finds that companies with better environmental, social, and governance (ESG) performance are more attractive to SRI mutual funds. Moreover, SRI mutual funds positively impact their investee firms' ESG performance through various channels such as ownership structure, board members' international experience, and social media attention.
INTERNATIONAL REVIEW OF FINANCIAL ANALYSIS
(2023)
Article
Biochemistry & Molecular Biology
Fanhong Wang, Kun Qiao, Huanhuan Wang, Hong Wang, Tuanyao Chai
Summary: In this study, a Mn-CDF transporter called TuMTP8 was identified in diploid wheat. It was found to play a crucial role in regulating Mn distribution and homeostasis, as well as participating in the intracellular transport and storage of excess Mn, thereby conferring Mn tolerance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Saskia Schott, Robina Scheuer, Francesca Ermoli, Timo Glatter, Elena Evguenieva-Hackenberg, Andreas Diepold
Summary: Many Gram-negative pathogens use type III secretion system (T3SS) to translocate virulence-promoting effector proteins into host cells. The activation of T3SS causes a reduction in bacterial growth and division known as secretion-associated growth inhibition (SAGI). In this study, a ParDE-like toxin-antitoxin system was identified on the virulence plasmid of Yersinia enterocolitica, which plays a role in the maintenance of virulence plasmid and reduction of plasmid loss. However, it is not directly involved in the SAGI or the activity of T3SS.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Endocrinology & Metabolism
Kanae Ogawa, Khaleque N. Khan, Haruo Kuroboshi, Akemi Koshiba, Koki Shimura, Tatsuro Tajiri, Shigehisa Fumino, Hiroyuki Fujita, Tomoharu Okubo, Yoichiro Fujiwara, Go Horiguchi, Satoshi Teramukai, Akira Fujishita, Kyoko Itoh, Sun-Wei Guo, Jo Kitawaki, Taisuke Mori
Summary: This study aimed to clarify the mechanistic link between neonatal uterine blood (NUB) and the development of early-onset endometriosis. The results showed no evidence supporting the role of NUB in early-onset endometriosis and found a significantly lower accumulation of immunocompetent cells in neonatal endometria. The expression of ovarian steroid receptors, decidualization, and proliferative and angiogenic activity were variable in neonatal endometria.
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY
(2023)
Article
Chemistry, Medicinal
Hyun Su Kim, Hyejin Kong, Taewoo Kim, Changjin Lim, Seungbeom Lee, Seok-Ho Kim, Young-Ger Suh
Summary: This study aimed to elucidate the structural congeners of natural izenamides A, B, and C (1-3) responsible for cathepsin D (CTSD) inhibition. The biologically important core structures of the synthesized izenamide variants were identified. It was confirmed that the natural statine (Sta) unit is a requisite core structure for CTSD inhibition, and statine-incorporated izenamide C variant and 18-epi-izenamide B variant showed more potent CTSD-inhibitory activities than natural izenamides.
Article
Engineering, Environmental
Guang Yang, Juan Li, Zhiyang Cheng, Qingwen Qin, Hongwei Zhang, Na Lu, Jie Wang
Summary: This study applied a ferrate in-situ oxidation-coagulation ultrafiltration integrated system to analyze the impact of meso-particles on membrane fouling behavior in drinking water treatment. The research found that meso-particles between 20 and 100 nm were the main components during the initial oxidation phase, and small molecules gradually formed meso-particles of 200-500 nm through flocculation. The statistical correlation between multiscale particles and membrane flux and resistance showed that meso-particles in the range of 20-100 nm primarily contributed to irreversible fouling, while reversible fouling was related to the synergy of meso- and micro-particles. The study also investigated the fouling behavior of meso-particles on the membrane surface and the structural evolution of the fouling layer through interfacial interaction, UV-vis reflectance spectroscopy, and molecular dynamics simulation.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Thermodynamics
Lan Yang, Changshi Liu
Summary: This paper proposes a simplified single diode model that can effectively calculate the relationship between current and voltage in solar cells, and represents the correlation with power conversion efficiency through one parameter. The proposed model is found to have a very good match with experimental results through evaluation of 30 sets of experimental data. The first important research finding indicates that power conversion efficiency is independent of the solar cell connection in series, due to the parallel conductance being independent of the solar cell connection in series. The major contribution of this paper is the discovery of the mathematical relationship between power conversion efficiency and parallel conductance, which provides a promising direction for researchers to discover advanced solar cells.
Article
Biochemistry & Molecular Biology
Daniel Schmidt, Nikolaus Falb, Ilenia Serra, Marzia Bellei, Vera Pfanzagl, Stefan Hofbauer, Sabine Van Doorslaer, Gianantonio Battistuzzi, Paul G. Furtmuller, Christian Obinger
Article
Biochemistry & Molecular Biology
Andrea Dali, Thomas Gabler, Federico Sebastiani, Alina Destinger, Paul Georg Furtmueller, Vera Pfanzagl, Maurizio Becucci, Giulietta Smulevich, Stefan Hofbauer
Summary: Coproporphyrin ferrochelatases (CpfCs) are enzymes that catalyze the penultimate step in the coproporphyrin-dependent heme biosynthesis pathway. The discovery of the correct substrate for these ferrochelatases and the characterization of their binding mode provide valuable insights into the iron insertion process. This knowledge is essential for understanding the preconditions and mechanisms of iron insertion in CpfCs.
Article
Biochemistry & Molecular Biology
Federico Sebastiani, Chiara Baroni, Gaurav Patil, Andrea Dali, Maurizio Becucci, Stefan Hofbauer, Giulietta Smulevich
Summary: Monoderm bacteria accumulate heme b through the coproporphyrin-dependent biosynthesis pathway. The decarboxylation of propionate groups in coproheme by coproheme decarboxylase (ChdC) is a stepwise process. H-bond interactions in the pocket of ChdCs play a crucial role in stabilizing the active site and enzyme functionality, which were evaluated through characterization of mutants complexed with coproheme and heme b via spectroscopies.
Article
Biochemistry & Molecular Biology
Daniel R. Ramos, Paul G. Furtmueller, Christian Obinger, Angeles Pena-Gallego, Ignacio Perez-Juste, J. Arturo Santaballa
Summary: Electronic structure calculations using DFT were conducted to examine the influence of water molecules and protonation on the heme group of peroxidases in different redox and spin states. The study discusses shared geometries, spectroscopic properties, and thermodynamics of peroxidases. Computed Gibbs free energies suggest that the corresponding aquo complexes are not thermodynamically stable, supporting the five-coordinate Fe(III) center in native ferric peroxidases with a non-bonding water molecule. Protonation of the ferryl oxygen of compound II is found to be necessary and computed Gibbs free energies reveal pK(a) values of approximately 8.5-9.0 for compound II.
Article
Chemistry, Analytical
Jürgen Beck, Matthias Biechele, Christoph Repik, Petra Gruber, Paul G. Furtmueller, Rainer Hahn
Summary: This study systematically compared the elution behavior of plasmid DNA on three common anion exchange resins. It was found that plasmid DNA consistently elutes at a characteristic salt concentration in linear gradient elution, and elutes only above this concentration in isocratic elution. Structural analysis supported this explanation.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Gaurav Patil, Hanna Michlits, Paul G. Furtmueller, Stefan Hofbauer
Summary: Coproheme decarboxylases (ChdCs) are enzymes involved in the biosynthesis of heme. This study focuses on the second part of the decarboxylation reaction catalyzed by ChdCs, which has not been previously studied. The researchers optimized the production and purification of a intermediate compound called monovinyl, monopropionate deuteroheme (MMD), and used it to study the reaction mechanism. The results indicate that the second part of the reaction is similar to the first part, with slight differences in the active site architecture and H-bonding network.
Article
Biochemistry & Molecular Biology
Bettina Motycka, Florian Csarman, Melanie Rupp, Karoline Schnabel, Gabor Nagy, Kwankao Karnpakdee, Stefan Scheiblbrandner, Rupert Tscheliessnig, Chris Oostenbrink, Michal Hammel, Roland Ludwig
Summary: This study investigates the role of two crucial amino acids in cellobiose dehydrogenase (CDH) in the interaction between the dehydrogenase and cytochrome domains, as well as in the interdomain electron transfer. The results suggest that these two residues play an essential role in shaping the domain interaction and facilitating electron transfer.
Article
Chemistry, Medicinal
Mateo Barria-Urenda, Alvaro Ruiz-Fernandez, Carlos Gonzalez, Chris Oostenbrink, Jose Antonio Garate
Summary: This study systematically investigated the adsorption process of amino acids onto graphene through free-energy calculations. A linear correlation was found between the free energy and the change in solvent accessible surface area, which could be used to predict the adsorption free energy of pentapeptides. Graphene oxidation resulted in a loss of favorability for amino acid adsorption.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2023)
Article
Biochemistry & Molecular Biology
Federico Sebastiani, Andrea Dali, Diego Javier Alonso de Armino, Lorenzo Campagni, Gaurav Patil, Maurizio Becucci, Stefan Hofbauer, Dario A. Estrin, Giulietta Smulevich
Summary: This study focuses on the carbon monoxide adducts of the wild-type and selected variants of the coproheme decarboxylase from actinobacterial Corynebacterium diphtheriae complexed with coproheme, monovinyl monopropionyl deuteroheme (MMD), and heme b. The results show that the wild-type coproheme-CO adduct is characterized by two CO conformers, hydrogen-bonded and weak polar interaction with the distal cavity. The absence of the H118 residue leads to the formation of non-H-bonded CO species. In addition, CO binding to reversed heme b and heme d is also investigated in this work.
JOURNAL OF INORGANIC BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Thomas Gabler, Andrea Dali, Federico Sebastiani, Paul Georg Furtmueller, Maurizio Becucci, Stefan Hofbauer, Giulietta Smulevich
Summary: Understanding the reaction mechanism of enzymes is challenging, but studying model substrates can provide valuable information. In this study, we investigated the mechanism of ferrous iron incorporation in a bacterial enzyme complex and discovered the role of hydrogen bond interactions in this process.
Review
Biochemistry & Molecular Biology
Nikolaus Falb, Gaurav Patil, Paul G. Furtmueller, Thomas Gabler, Stefan Hofbauer
Summary: The coproporphyrin dependent heme biosynthesis pathway is predominantly used by Gram-positive bacteria. This pathway is of interest for basic research as it relates to medical biotechnology and the development of new antibiotic targets against Gram-positive superbugs. A review of the accumulated structural data on the enzymes involved in this pathway is provided, highlighting the need for further analysis and future research to gain a comprehensive understanding of prokaryotic heme biosynthesis.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Oriol Gracia Carmona, Majd Lahham, Peter Poliak, Dominic Goj, Eva Friesser, Silvia Wallner, Peter Macheroux, Chris Oostenbrink
Summary: This study investigates the substrate specificity of the FsqB enzyme and discovers the role of tyrosine and aspartate residues in fixing the substrate close to the isoalloxazine ring of the cofactor. The combination of experimental and theoretical approaches helps solve the mystery behind the activity of FsqB enzyme.
JOURNAL OF MOLECULAR RECOGNITION
(2023)
