Article
Oncology
Chen Hao Lo, Gemma Shay, Jeremy J. McGuire, Tao Li, Kenneth H. Shain, Jun Yong Choi, Rita Fuerst, William R. Roush, Anna M. Knapinska, Gregg B. Fields, Conor C. Lynch
Summary: Multiple myeloma induces MMP13 expression in bone stromal cells, which contributes to disease progression. MMP13-deficient mice show higher survival rates and lower levels of bone destruction, indicating the critical role of bone stromal-derived MMP13 catalytic activity in osteoclastogenesis, bone destruction, and disease progression.
Article
Medicine, General & Internal
Stanislava Dimitrova Popova-Belova, Mariela Gencheva Geneva-Popova, Krasimir Iliev Kraev, Velichka Zaharieva Popova
Summary: This retrospective case-control study found that the serum and synovial levels of CatG and CatK were significantly higher in patients with psoriatic arthritis (PsA) and were positively associated with disease activity indicators. These findings suggest that serum and synovial levels of CatG and CatK could serve as potential biomarkers for assessing disease activity in psoriatic arthritis.
Article
Materials Science, Multidisciplinary
Shuang He, Yu-Yang Gao, Ze-Yong Zhao, Sheng-Chao Huang, Zi-Xun Chen, Cong Deng, Yu-Zhong Wang
Summary: Phytic acid-amino acid salts derived from biomass material were found to effectively enhance the flame retardancy of polypropylene, with arginine showing the highest efficiency in increasing flame retardancy, while lysine significantly reducing the fire hazard of PP during burning.
ACS APPLIED POLYMER MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Anne Bernhardt, Jana Bacova, Uwe Gbureck, Michael Gelinsky
Summary: The study found that the effects of Cu2+ on osteoclasts varied depending on their differentiating or mature state. High TRAP activity does not necessarily correlate with high resorption capacity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Endocrinology & Metabolism
Bongjin Shin, Henry C. Hrdlicka, Anne M. Delany, Sun-Kyeong Lee
Summary: The miR-29-3p family of microRNAs plays a crucial role in osteoclastogenesis and bone homeostasis by targeting Calcr and Ctsk. Inhibition of miR-29-3p activity leads to increased bone volume due to reduced bone resorption. This study reveals an interplay between CALCR and CTSK in osteoclastic bone resorption regulated by miR-29-3p.
Article
Multidisciplinary Sciences
Maxime Deraspe, Sebastien Boisvert, Francois Laviolette, Paul H. Roy, Jacques Corbeil
Summary: Protein identification is a computationally intensive step in genomics studies. This study introduces kAAmer, a protein database engine that efficiently identifies proteins and allows for flexible protein annotations. The database can also be used as a microservice, hosted and queried remotely.
SCIENTIFIC REPORTS
(2022)
Article
Geriatrics & Gerontology
Xiangkun Meng, Zhe Huang, Aiko Inoue, Hailong Wang, Ying Wan, Xueling Yue, Shengnan Xu, Xueying Jin, Guo-Ping Shi, Masafumi Kuzuya, Xian Wu Cheng
Summary: This study suggests that cathepsin K (CTSK) plays a significant role in muscle wasting in cancer-related cachexia by mediating the ubiquitination of insulin receptor substrate 1 (IRS1).
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2022)
Article
Oncology
Rehan Imad, Zeeshan Sheikh, Mallikarjuna Rao Pichika, Mak Kit-Kay, Rehan Ahmed Siddiqui, Syed Nudrat Nawaid Shah, Spoorthi Banavar, Jukka Matinlinna, Seow Liang Lin, Umer Daood
Summary: By analyzing the function of the new K21 molecule in the invasive process of oral squamous cell carcinoma, it was found that the K21-based drug delivery system can reduce the overexpression of CatK, leading to a decrease in the migration and adhesion ability of oral squamous cell carcinoma cells.
EXPERIMENTAL CELL RESEARCH
(2023)
Review
Cell Biology
Qiaochu Li, Thorsten Hoppe
Summary: Mitochondria are essential for energy production and cellular signaling. Maintaining mitochondrial homeostasis is crucial for cell function and survival. Amino acid metabolism plays a key role in energy production and protein quality control in both mitochondria and cytoplasm. Impaired amino acid metabolism is associated with mitochondrial diseases and diseases with mitochondrial dysfunction such as cancer.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Fisheries
Ting Zhang, Jingting Yao, Dongpo Xu, Guohua Lv, Haibo Wen
Summary: This study investigated the osmolality and osmoregulatory mechanisms of Solenaia oleivora under high salinity stress. The results showed that S. oleivora is an osmoconformer, and changes in inorganic ions, FAA, NKA, and gill structure play an important role in its osmoregulation.
Article
Pharmacology & Pharmacy
Karen Perez-Carreon, Luz Maria Martinez, Marcelo Videa, Jorge Cruz-Angeles, Jimena Gomez, Emilio Ramirez
Summary: This study determined the effective molar ratio of arginine and lysine to folic acid (FA) that maximized solubility using conductimetric titration. Binary formulations were prepared, resulting in an amorphous system with significantly increased solubility and morphology dependent on the preparation method. These findings are important for enhancing solubility and scaling purposes.
Article
Food Science & Technology
Yufeng Li, Jinsong Wang, Xiping Zhu, Lijun Tan, Dan Xie, Wenjing Xu, Yunlei Gui, Yong Zhao, Jing Jing Wang
Summary: This study developed a new method combining basic electrolyzed water with ultrasonic treatment to modify Antarctic krill proteins (AKPs). The modified AKPs showed improved oxidative stability, solubility, hydrophobicity, foam stability, foam capacity, emulsifying properties, and in vitro digestibility. This method has great potential for the production of high nutritional marine protein products.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Chemistry, Physical
Taegun Park, Taehyeon Kim, Jongwon Han, Sangwoo Lim
Summary: The improvement of Si3N4 etching kinetics and suppression of oxide regrowth are crucial in the selective Si3N4 etching process for the fabrication of 3D NAND devices on multi-layered Si3N4/SiO2 structures. By reducing the concentration of H3PO4, oxide regrowth was suppressed and 30 wt% H3PO4 showed the highest Si3N4/SiO2 etching selectivity. Additionally, introducing amino acids with hydrophilic side chains into 30 wt% H3PO4 increased the Si3N4 etching rate and suppressed oxide regrowth.
APPLIED SURFACE SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Shun Sato, Kana Yamamoto, Moeno Ito, Katsutoshi Nishino, Takanao Otsuka, Kazuhiro Irie, Masaya Nagao
Summary: Two types of cathepsin K inhibitors were identified in the methanol extract of Chamaecrista nomame. One type can inhibit both cathepsin K and B, while the other type only inhibits cathepsin K, suggesting allosteric binding. The combination of these inhibitors with other allosteric inhibitors enhances their inhibitory activity, providing important insights for designing new allosteric inhibitory drugs.
Article
Chemistry, Physical
Santanu Santra, Shakuntala Dhurua, Madhurima Jana
Summary: Amino acids with basic side chains are known to increase the stability of proteins in their native-folded state, however, the efficiency and molecular mechanism behind this process are still controversial. A study using molecular dynamics simulations found that arginine, histidine, and lysine solutions have varying effects on the hydration and conformational stability of the insulin monomer. Arginine was observed to influence insulin stability by enhancing the exclusion of water molecules from the protein surface, leading to more rigid conformations compared to histidine and lysine.
JOURNAL OF CHEMICAL PHYSICS
(2021)
Article
Biochemistry & Molecular Biology
Zahira Tber, Mylene Wartenberg, Jean-Eddy Jacques, Vincent Roy, Fabien Lecaille, Dawid Warszycki, Andrzej J. Bojarski, Gilles Lalmanach, Luigi A. Agrofoglio
BIOORGANIC & MEDICINAL CHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Mylene Wartenberg, Ahlame Saidi, Mathieu Galibert, Alix Joulin-Giet, Julien Burlaud-Gaillard, Fabien Lecaille, Christopher J. Scott, Vincent Aucagne, Agnes F. Delmas, Gilles Lalmanach
Article
Multidisciplinary Sciences
Ahlame Saidi, Mariana Kasabova, Lise Vanderlynden, Mylene Wartenberg, Ghania Hounana Kara-Ali, Daniel Marc, Fabien Lecaille, Gilles Lalmanach
SCIENTIFIC REPORTS
(2019)
Article
Physiology
Pierre-Marie Andrault, Andrea C. Schamberger, Thibault Chazeirat, Damien Sizaret, Justine Renault, Claudia A. Staab-Weijnitz, Elisabeth Hennen, Agnes Petit-Courty, Mylene Wartenberg, Ahlame Saidi, Thomas Baranek, Serge Guyetant, Yves Courty, Oliver Eickelberg, Gilles Lalmanach, Fabien Lecaille
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Anne-Sophie Lamort, Yveline Hamon, Cezary Czaplewski, Artur Gieldon, Seda Seren, Laurent Coquet, Fabien Lecaille, Adam Lesner, Gilles Lalmanach, Francis Gauthier, Dieter Jenne, Brice Korkmaz
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Biochemistry & Molecular Biology
Fabien Lecaille, Thibault Chazeirat, Krzysztof K. Bojarski, Justine Renault, Ahlame Saidi, V. Gangadhara N. V. Prasad, Sergey Samsonov, Gilles Lalmanach
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2020)
Article
Biochemistry & Molecular Biology
Mylene Wartenberg, Pierre-Marie Andrault, Ahlame Saidi, Paul Bigot, Lydie Nadal-Desbarats, Fabien Lecaille, Gilles Lalmanach
FREE RADICAL BIOLOGY AND MEDICINE
(2020)
Review
Biochemistry & Molecular Biology
Gilles Lalmanach, Ahlame Saidi, Paul Bigot, Thibault Chazeirat, Fabien Lecaille, Mylene Wartenberg
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Chemistry, Applied
Thibault Chazeirat, Sophie Denamur, Krzysztof K. Bojarski, Pierre-Marie Andrault, Damien Sizaret, Fuming Zhang, Ahlame Saidi, Marine Tardieu, Robert J. Linhardt, Francois Labarthe, Dieter Bromme, Sergey A. Samsonov, Gilles Lalmanach, Fabien Lecaille
Summary: The study revealed a correlation between HS and CatV in the lungs of MPS patients, with HS level positively associated with the severity of respiratory symptoms. The excessive HS might impair CatV-mediated ECM remodeling and lung tissue homeostasis, contributing to respiratory disorders in MPS diseases.
CARBOHYDRATE POLYMERS
(2021)
Review
Oncology
Gilles Lalmanach, Mariana Kasabova-Arjomand, Fabien Lecaille, Ahlame Saidi
Summary: Cystatin M/E, a low molecular mass protein, exhibits both tumor-suppressing and tumor-promoting effects in various cancers. Understanding the regulatory mechanisms of Cystatin M/E and its expression and activity in tumorigenesis is crucial in maintaining proteolytic balance and addressing its imbalance in cancer.
Article
Biochemical Research Methods
Ahlame Saidi, Mylene Wartenberg, Jean-Baptiste Madinier, Guy Ilango, Seda Seren, Brice Korkmaz, Fabien Lecaille, Vincent Aucagne, Gilles Lalmanach
Summary: The near-infrared fluorescent substrate-based probe (pro3-SBP) is a convenient tool for monitoring hPR3 activity, showing high specificity and sensitivity in detecting human neutrophil activation processes. It can be successfully hydrolyzed by activated human neutrophils and provides a strong signal under confocal microscopy.
BIOCONJUGATE CHEMISTRY
(2021)
Article
Veterinary Sciences
Ahlame Saidi, Pauline Gaboriaud, Anne-Christine Lalmanach, Lise Vanderlynden, Aurelie Fessard, Pauline Vettori, Genevieve Fort, Rodrigo Guabiraba, Catherine Schouler, Fabrice Laurent, Edouard Guitton, Fabien Lecaille, Francoise I. Bussiere, Gilles Lalmanach
Summary: During coccidiosis infection, host cysteine cathepsin activity increases, potentially contributing to the alteration of intestinal proteolytic balance. Furthermore, cysteine cathepsins may be involved in inflammation during coccidiosis by proteolytically inactivating up-regulated avian beta-defensins.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Biochemical Research Methods
K. K. Bojarski, J. Sage, G. Lalmanach, F. Lecaille, S. A. Samsonov
Summary: This study investigated the interactions between human cysteine cathepsins and various types of glycosaminoglycans (GAGs). Molecular docking simulations and molecular dynamics analysis revealed that electrostatics play a crucial role in driving the cathepsin-GAG interactions, and the length of GAG chains significantly affects the strength of these interactions. Furthermore, binding of GAGs at different regions on cathepsins surface has varying effects on the enzymatic activity, depending on the cathepsin and GAG type.
JOURNAL OF MOLECULAR GRAPHICS & MODELLING
(2022)
Article
Biochemistry & Molecular Biology
Paul Bigot, Simon Chesseron, Ahlame Saidi, Damien Sizaret, Christelle Parent, Agnes Petit-Courty, Yves Courty, Fabien Lecaille, Gilles Lalmanach
Summary: Chronic obstructive pulmonary disease (COPD) is an irreversible disease mainly caused by smoking. The protein occludin, involved in the cohesion of epithelial tight junctions, is found to be decreased in lung biopsies of smokers and correlates with smoking history, COPD grades, and cathepsin S (CatS) activity. Cigarette smoke-elicited CatS induces an alteration of epithelial integrity via the proteolytic injury of occludin, highlighting the emerging role of CatS in smoking-related lung diseases and the relevance of targeting CatS in the treatment of emphysema and COPD.
Review
Biochemistry & Molecular Biology
Alexis David, Thibault Chazeirat, Ahlame Saidi, Gilles Lalmanach, Fabien Lecaille
Summary: Mucopolysaccharidosis (MPS) is a group of inherited lysosomal storage disorders caused by enzyme defects in glycosaminoglycan (GAG) metabolism. Accumulation of GAGs leads to tissue dysfunctions during childhood, resulting in premature death. Understanding the molecular mechanisms and exploring the role of cysteine cathepsins in MPS may improve current therapies. This review summarizes the current understanding of MPS disorders, their management, and the interplay between GAGs and cysteine cathepsins.