Article
Multidisciplinary Sciences
Takeshi Tsusaka, Juan A. Oses-Prieto, Christina Lee, Brian C. DeFelice, Alma L. Burlingame, Emily L. Goldberg
Summary: Ketone bodies are produced in the liver during glucose scarcity and act as alternative energy sources. Beta-hydroxybutyrate (BHB) has been identified as a signaling molecule, and lysine beta-hydroxybutyrylation (Kbhb) as a newly discovered post-translational modification. Kbhb is metabolically sensitive and found on proteins in multiple cellular compartments, suggesting its importance in ketone body-regulated physiology. However, caution is needed when using the H3K9bhb antibody to interpret gene regulation data, as it may recognize other modifications besides beta-hydroxybutyrylation.
Article
Biochemistry & Molecular Biology
Fabian A. Garces, Jessica F. Scortecci, Robert S. Molday
Summary: This study investigated the molecular properties of ABCA4 variants with disease-causing missense mutations, categorizing them into three classes based on their expression levels, ATPase activities, and substrate binding capabilities. Understanding these classifications can provide insights into genotype-phenotype correlations and aid in evaluating therapeutic options for Stargardt macular degeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Chancievan Thangaratnarajah, Jan Rheinberger, Cristina Paulino, Dirk J. Slotboom
Summary: Energy-coupling factor (ECF)-type transporters are small, asymmetric membrane protein complexes that import micronutrients into bacterial cells. Recent research suggests that during the transport process, protein-induced membrane deformations facilitate the toppling of the S component.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Pharmacology & Pharmacy
Xing-Duo Dong, Meng Zhang, Chao-Yun Cai, Qiu-Xu Teng, Jing-Quan Wang, Yi-Ge Fu, Qingbin Cui, Ketankumar Patel, Dong-Tao Wang, Zhe-Sheng Chen
Summary: This study investigated the potential drug resistance mechanism of the KRAS-G12C inhibitor ARS-1620 associated with ABCB1. The findings indicated that ARS-1620 could be competitively pumped out by ABCB1 in cancer cells and significantly stimulated the ATPase activity of ABCB1. These findings suggest that the potential influence of ARS-1620-related cancer therapy on ABCB1-overexpressing cancer cells should be considered in future clinical applications.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Biology
Fangyu Liu, James Lee, Jue Chen
Summary: The ATP-binding cassette (ABC) transporter family is diverse and contains thousands of members. ABCA4 is a eukaryotic importer that transports retinal and removes toxic retinoids, mutations in which lead to impaired vision. Despite years of study, the molecular mechanism of ABCA4 remains unknown.
Review
Endocrinology & Metabolism
Huimei Wei, Peng Huo, Shun Liu, Hua Huang, Shun Zhang
Summary: PCOS is a lifelong disorder affecting a significant number of women and associated with increased risk of various diseases. Posttranslational modifications play a crucial role in PCOS development, and understanding their roles and mechanisms can provide insights for future research and treatment.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Review
Plant Sciences
Umar Gani, Ram A. Vishwakarma, Prashant Misra
Summary: This review summarizes the recent updates in the area of transporters of plant secondary metabolites and their applied aspects in metabolic engineering. Plants have evolved biosynthetic pathways to produce diverse secondary metabolites with various functions in ecological interactions. Transporter proteins, especially ATP binding cassette (ABC) and multidrug and toxic compound extrusion (MATE) families, play a crucial role in membrane-mediated transport of plant secondary metabolites.
PLANT CELL REPORTS
(2021)
Review
Biochemistry & Molecular Biology
Keith E. Keenan, Derek K. Zachman, Matthew D. Hirschey
Summary: Protein modifications play a significant role in cell biology across various organisms. Discoveries in the early 20th century challenged traditional definitions of amino acids, and the advent of mass-spectrometry-based proteomics in the latter part led to a rapid increase in known protein modifications, with over 500 identified today. New computational tools in data science, machine learning, and artificial intelligence are expected to further advance the discovery and understanding of protein modifications in the future.
Article
Plant Sciences
Jennifer Schwestka, Julia Koenig-Beihammer, Yun-Ji Shin, Ulrike Vavra, Nikolaus F. Kienzl, Clemens Gruenwald-Gruber, Daniel Maresch, Miriam Klausberger, Elisabeth Laurent, Maria Stadler, Gabriele Manhart, Jasmin Huber, Manuela Hofner, Klemens Vierlinger, Andreas Weinhaeusel, Ines Swoboda, Christoph J. Binder, Wilhelm Gerner, Florian Grebien, Friedrich Altmann, Lukas Mach, Eva Stoeger, Richard Strasser
Summary: The receptor binding domain (RBD) of the SARS-CoV-2 spike protein is crucial in virus-host cell interaction and infection, with plant-produced RBD potentially leading to false positive reactions in serological assays.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemical Research Methods
Juan Carlos Aledo
Summary: The software package ptm is designed for studying methionine sulfoxidation, but can be used for analyzing other posttranslational modifications as well. By integrating information from multiple databases, ptm allows for programmatic analysis of thousands of proteins and addresses site- and protein-centric hypotheses related to PTMs.
Article
Genetics & Heredity
Anna Schmuecker, Bingkun Lei, Zdravko J. Lorkovic, Matias Capella, Sigurd Braun, Pierre Bourguet, Olivier Mathieu, Karl Mechtler, Frederic Berger
Summary: The selection of C-terminal motifs contributes to the evolution of distinct histone H2A variants, with cell cycle dependent kinases playing a crucial role. Interference between the functions carried by these motifs led to their mutual exclusion and the evolution of different classes of H2A variants.
Article
Microbiology
Konstantinos Drousiotis, Reyme Herman, Judith Hawkhead, Andrew Leech, Anthony Wilkinson, Gavin H. Thomas
Summary: The bacterium Shewanella sp. ANA-3 can utilize a rare sugar, iota-arabinofuranose, found in lignocellulosic biomass. The substrate binding protein GafASw has been shown to specifically bind to this sugar, indicating a natural predilection of this bacterium for certain plant biomass components. The structure comparison with GafAEc suggests adaptations in the binding site that confer specificity for furanose over pyranose and for arabinose over galactose. This discovery provides insights for more efficient utilization of plant biomass in industrial biotechnology.
Article
Pharmacology & Pharmacy
Xinling Cui, Wei Mi, Zhishang Hu, Xiaoyu Li, Bo Meng, Xinyuan Zhao, Xiaohong Qian, Tao Zhu, Wantao Ying
Summary: Posttranslational modifications of antibody products have a significant impact on their quality attributes. This study aimed to comprehensively characterize the posttranslational modification status of antibody drug charge isomers (CIs) and investigate the effect of modifications on charge heterogeneity. Through structural characterization at different levels, the study revealed the dynamics of glycoforms and the importance of sialic acid modification in accounting for charge heterogeneity.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2022)
Article
Microbiology
Valentin Borgeat, Danielle Brandalise, Frederic Grenouillet, Dominique Sanglard
Summary: Candida lusitaniae is an opportunistic pathogen in humans that can rapidly acquire multidrug resistance, with the ABC transporter CDR1 playing an important role in azole resistance.
Review
Pharmacology & Pharmacy
Mitra Farnoodian, Devika Bose, Francesca Barone, Luke Mathew Nelson, Marisa Boyle, Bokkyoo Jun, Khanh Do, William Gordon, Marie-Audrey Kautzmann Guerin, Rasangi Perera, Jeff X. Ji, Tiziana Cogliati, Ruchi Sharma, Brian P. Brooks, Nicolas G. Bazan, Kapil Bharti
Summary: Stargardt maculopathy, caused by mutations in the ABCA4 gene, results in accumulation of lipofuscin in the retinal pigment epithelium, leading to RPE atrophy and vision loss. Previous research focused on photoreceptor mutations as the main cause of lipid homeostasis defects, but recent findings demonstrate that ABCA4 loss of function in the RPE itself contributes to these defects. Understanding lipid metabolism and signaling in the retina and RPE is crucial for developing effective treatments for this disease.
PHARMACOLOGY & THERAPEUTICS
(2023)