Article
Chemistry, Physical
Xue Li, Qingqing Liu, Haochen Zou, Jie Luo, Yujie Jiao, Haoxin Wang, Liangcheng Du, Yuemao Shen, Yaoyao Li
Summary: By heterologous expression of the recombinant pel cluster, we have revealed the roles of two FMN-dependent oxidoreductases, Pel1 and Pel3, and provided insights into the formation of the 5-5 bicyclic system in PoTeM biosynthesis.
Article
Biochemistry & Molecular Biology
Huan Yue, Jiasong Jiang, Anna J. Taylor, Aline De Lima Leite, Eric D. Dodds, Liangcheng Du
Summary: Lysobacter, particularly L. enzymogenes, known for producing lytic enzymes and bioactive metabolites, utilizes a previously uncharacterized synergistic strategy involving the LeLPMO10A-encoded chitin-binding protein and antifungal compounds colocalized in outer membrane vesicles when interacting with fungal prey. This study sheds light on the predatory interaction mechanism with fungi.
ACS CHEMICAL BIOLOGY
(2021)
Article
Biochemical Research Methods
Yaqian Yan, Haoxin Wang, Yuliang Song, Deyu Zhu, Yuemao Shen, Yaoyao Li
Summary: PoTeMs are a family of natural products containing a tetramic acid moiety and a polycyclic system, displaying cytotoxic activity against human cancer cell lines. Manipulating the biosynthesis of PoTeMs by swapping modification genes between different pathways can facilitate the engineered biosynthesis of other PoTeMs in the future.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Plant Sciences
Long Lin, Zixiang Yang, Min Tao, Danyu Shen, Chuanbin Cui, Pingping Wang, Limin Wang, Maofeng Jing, Guoliang Qian, Xiaolong Shao
Summary: This study demonstrated that OH11 could prevent plant infection by Phytophthora pathogens through multiple mechanisms, making it a promising alternative resource for promoting plant resistance to multiple Phytophthora pathogens.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Chemistry, Organic
Haochen Zou, Xin Xia, Qiushuang Xu, Haoxin Wang, Yuemao Shen, Yaoyao Li
Summary: The structural diversity of PoTeMs is mainly generated by cyclases and cytochrome P450s. In Saccharopolyspora hirsuta, six new cytotoxic PoTeMs, including the first examples of oxidized one-ring PoTeMs, were discovered through combinatorial reassembling and heterologous expression.
Article
Agriculture, Multidisciplinary
Amanda Lynn Miller, Shanren Li, Catherine D. Eichhorn, Yongbiao Zheng, Liangcheng Du
Summary: In this study, the first siderophore, lysochelin, and its biosynthetic gene cluster were identified from Lysobacter enzymogenes. It was found that lysochelin, along with antifungal compounds HSAF and its analogues, are crucial for the disease control activity and survival of Lysobacter under oxidative stresses caused by excess iron. The production of these molecules is greatly affected by iron concentration.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Lingjun Yu, Hui Li, Zaichun Zhou, Fengquan Liu, Liangcheng Du
Summary: PoTeMs are a rapidly growing family of antibiotic natural products with iron-chelating properties that can protect DNA from oxidative damage, helping bacteria survive under oxidative stress.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Agriculture, Multidisciplinary
Rongxian Hou, Kaihuai Li, Baodian Guo, Yangyang Zhao, Chaohui Li, Bao Tang, Weibo Sun, Bo Wang, Wenchan Chen, Cong Sheng, Jialiang Kan, Yancun Zhao, Fengquan Liu
Summary: This study identified the potential target of heat-stable antifungal factor (HSAF) in fungi and found that HSAF binds to specific sites on VpVeb1, resulting in decreased virulence of V. pyri.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Organic
Huan Yue, Liangcheng Du
Summary: This study reveals the important role of hsaf-orf1 in the regulation and biosynthesis of HSAF and its analogs by controlling the import of sugars, which induce antifungal production.
Article
Plant Sciences
Kaihuai Li, Rongxian Hou, Xue Zhou, Chunlan Xiong, Cheng Li, Yong Wang, Fengquan Liu
Summary: In this study, a potential regulator of HSAF biosynthesis, RecX, was identified. Deletion of recX increased HSAF production, while overexpression of recX suppressed HSAF production. It was found that RecX directly binds to the promoter region of the lafB gene to inhibit its transcription and thus decrease HSAF production in L. enzymogenes.
PHYTOPATHOLOGY RESEARCH
(2023)
Article
Chemistry, Organic
Luo Jie, Yan Yaqian, Wang Haoxin, Li Yaoyao
Summary: The artificial hybrid polycyclic tetramate macrolactam (PoTeM) gene cluster was constructed using combinatory biosynthesis approach, and two PoTeMs were successfully isolated. The chemical structures of the compounds were determined and their antibacterial and cytotoxic activities were tested.
CHINESE JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Chemistry, Organic
Yaqian Yan, Haoxin Wang, Yaoyao Li
Summary: A new polycyclic tetramate macrolactam was isolated from a recombinant strain of Streptomyces. The chemical structure and biological activities of the compound were investigated, shedding light on the biosynthesis pathway of polycyclic tetramate macrolactams.
CHINESE JOURNAL OF ORGANIC CHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Huan Yue, Amanda Lynn Miller, Vimmy Khetrapal, Vishakha Jayaseker, Stephen Wright, Liangcheng Du
Summary: This article reviews the progress in Lysobacter natural product research over the past ten years, including molecular mechanisms for biosynthesis, regulation and mode of action, genome mining of cryptic biosynthetic gene clusters, and metabolic engineering using synthetic biology tools.
NATURAL PRODUCT REPORTS
(2022)
Article
Microbiology
Xi Shen, Bingxin Wang, Nianda Yang, Lulu Zhang, Danyu Shen, Huijun Wu, Ying Dong, Ben Niu, Shan-Ho Chou, Gerardo Puopolo, Jiaqin Fan, Guoliang Qian
Summary: The study revealed that the soilborne biocontrol bacterium Lysobacter enzymogenes OH11 uses T4SS as the main contact-dependent weapon against other soilborne bacteria, decreasing their activity while protecting carrots from infection. This highlights the crucial role of T4SS in the killing behavior of L. enzymogenes and its impact on interactions in the soil microbiome.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Plant Sciences
Jinping Yang, Yunci Qi, Joshua A. V. Blodgett, Timothy A. Wencewicz
Summary: This study provides detailed insights into the biosynthetic mechanism of polycyclic tetramate macrolactams (PTMs), particularly the role of P450 monooxygenases encoded in PTM biosynthetic gene clusters (BGCs). The multifunctional P450 monooxygenase CftA is shown to catalyze the conversion of ikarugamycin to clifednamide A or clifednamide C through C-H bond activation. The complete structure of clifednamide C is also revealed. These findings expand our understanding of PTM biosynthesis and the customization of PTM natural products.
JOURNAL OF NATURAL PRODUCTS
(2022)
