Journal
ANALYST
Volume 140, Issue 7, Pages 2493-2503Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c4an01982b
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Funding
- NSERC Canada
- NSF [CHE-1112433, DMR-0619759, DMR-0537588]
- Division Of Chemistry [1112433] Funding Source: National Science Foundation
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A recent upgrade to the optics configuration of a thermal source FTIR microscope equipped with a focal plane array detector has enabled rapid acquisition of high magnification spectrochemical images, in transmission, with an effective geometric pixel size of similar to 1 x 1 mu m(2) at the sample plane. Examples, including standard imaging targets for scale and accuracy, as well as biomedical tissues and microorganisms, have been imaged with the new system and contrasted with data acquired at normal magnification and with a high magnification multi-beam synchrotron instrument. With this optics upgrade, one can now conduct rapid biodiagnostic ex vivo tissue imaging in-house, with images collected over larger areas, in less time (minutes) and with comparable quality and resolution to the best synchrotron source FTIR imaging capabilities.
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