Article
Biochemistry & Molecular Biology
Debabrata Sinha, Tushar Chakraborty, Debasmita Sinha, Asim Poddar, Rajagopal Chattopadhyaya, Subrata Sau
Summary: The study focused on the interactions between the anti-anti-sigma factor and an anti-sigma factor in Staphylococcus aureus, revealing that the formation of the complex relies on hydrogen bonds and hydrophobic interactions, with a negative heat capacity change observed during the transition to a 2:2 complex formation. Additional experiments showed increased thermodynamic stability of the anti-anti-sigma factor in the presence of the anti-sigma factor, providing valuable insights into the structural and binding thermodynamics of these regulatory proteins.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2021)
Article
Biochemistry & Molecular Biology
Debasmita Sinha, Debabrata Sinha, Nilanjan Banerjee, Priya Rai, Soham Seal, Tushar Chakraborty, Subhrangsu Chatterjee, Subrata Sau
Summary: In this study, the impact of the critical residue Arg 23 in RsbW on its structure and function was investigated. By altering the amino acid at Arg 23, we found that rRsbW[R23A] was unable to interact and phosphorylate RsbV, and its stability and structure were significantly altered. However, rRsbW[R23A] still interacted with sigma(B). These results reveal the importance of Arg 23 in RsbW.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Biochemistry & Molecular Biology
Hossein Allahmoradi, S. Mohsen Asghari, Atieh Ahmadi, Elham Assareh, Mahboobeh Nazari
Summary: The study demonstrates the functional properties of the 118-126 region of FGF2 depend on the loop structure, and the peptide derived from this fragment encourages further preclinical investigations.
BIOCHEMICAL JOURNAL
(2022)
Article
Microbiology
Christine Pourciau, Helen Yakhnin, Archanna Pannuri, Mark G. Gorelik, Ying-Jung Lai, Tony Romeo, Paul Babitzke
Summary: Bacterial growth rate is dependent on protein synthesis, which is regulated by the post-transcriptional regulator CsrA in Escherichia coli. CsrA controls the expression of proteins that affect ribosome activity and RNA polymerase, optimizing protein production capacity. It represses the expression of certain proteins during exponential growth and derepresses others during stationary phase. This regulation supports global protein expression during active growth and limits it to a basal level when nutrients are limited. In addition, CsrA regulates the expression of membrane proteins that impact protein expression and membrane integrity. Understanding these regulatory mechanisms provides insights into bacterial growth control.
Article
Biochemistry & Molecular Biology
Lidia dos Passos Lima, Eliane Silva de Santana, Alan Pericles Rodrigues Lorenzetti, Rogerio Ferreira Lourenco, Lucas de Moraes Ceseti, Luana Riva, Ethel Bayer-Santos, Ana Laura Boechat, Regina Lucia Baldini, Chuck Shaker Farah, Tie Koide, Cristina Elisa Alvarez-Martinez
Summary: This study uncovers the regulatory mechanism of X. citri T6SS. Overexpression of a constitutively active EcfK activates the transcription factor TagK, which induces the expression of T6SS genes. TagK binds directly to T6SS gene promoters and promotes T6SS expression upon contact with amoeba cells.
MOLECULAR MICROBIOLOGY
(2022)
Article
Microbiology
Jeffrey K. J. Cheng, Tanja Dapa, Ivan Y. L. Chan, Thomas. O. O. MacCreath, Ross Slater, Meera Unnikrishnan
Summary: Pathogens like Clostridioides difficile use alternative transcriptional factors like sigma factor B (sigma(B)) to respond to different stresses. The regulatory role of RsbW in C. difficile physiology was investigated, and it was found that the RsbW mutant showed defects in spore and biofilm formation but increased adhesion to human gut epithelia. This study provides insights into the regulatory mechanisms and stress responses of C. difficile.
JOURNAL OF BACTERIOLOGY
(2023)
Article
Immunology
Jakub Drzmisek, Denisa Petrackova, Ana Dienstbier, Ivana Curnova, Branislav Vecerek
Summary: Bordetella pertussis produces the type III secretion system, and the novel chaperone BP2265 interacts specifically with the secreted T3SS regulator BtrA. In the absence of BP2265, secretion of BtrA and most T3SS substrates is severely impaired, while their production remains unaffected.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Yuetan Dou, Hiel Rutanhira, Norbert Schormann, Champion Deivanayagam, Hansel M. Fletcher
Summary: PG1659 is an anti-sigma factor that interacts with the sigma factor RpoE, inhibiting its transcriptional initiation and playing a regulatory role in P. gingivalis. Both protein-protein pull-down and bacterial two-hybrid assays confirmed the interaction between PG1659 and RpoE, highlighting the importance of the N-terminal domain of PG1659 for this interaction.
MOLECULAR ORAL MICROBIOLOGY
(2021)
Article
Energy & Fuels
Farhad Taghizadeh-Hesary, Ehsan Rasoulinezhad, Naoyuki Yoshino, Tapan Sarker, Nawazish Mirza
Summary: The study found that Russian energy exports to the Asia-Pacific region follow the Linder hypothesis, with economic growth positively influencing exports. Sanctions against Russia since 2014 have increased Russian energy exports to the region. To enhance regional pricing power, it is recommended to establish an energy trading hub in the Asia-Pacific region.
ENERGY STRATEGY REVIEWS
(2021)
Article
Biochemistry & Molecular Biology
Melissa C. Schofield, Daniela Q. Rodriguez, Amanda A. Kidman, Erin K. Cassin, Lia A. Michaels, Elizabeth A. Campbell, Peter A. Jorth, Boo Shan Tseng
Summary: In infections of cystic fibrosis airways, the gene mucA in Pseudomonas aeruginosa is crucial for bacterial viability; mutations in mucA can result in misregulation of AlgU and affect bacterial phenotype and disease outcomes.
MOLECULAR MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Andrew Blyth, Michael Ortiz, Allanah Merriman, Carlie Delaine, Briony Forbes
Summary: IGF-II plays important roles in fetal development and growth, but its overexpression in cancer promotes cancer cell survival. A residue in IGF-II has been found to influence its structural integrity and receptor binding, but has little effect on signaling outcome.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Bulat Fatkhullin, Alexander Golubev, Natalia Garaeva, Shamil Validov, Azat Gabdulkhakov, Marat Yusupov
Summary: The conserved protein RsfS plays a role in ribosome shutdown and cell survival during starvation, and is involved in the biogenesis of the large ribosomal subunit. In this study, the contribution of specific amino acid side chains at the interface between RsfS and uL14 in Staphylococcus aureus was investigated. The results suggest that the Y98 amino acid on RsfS surface may be a potential target for drug development and treatment of S. aureus infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Applied
Guoxing Liu, Juhua Leng, Qingyang Zhou, Zhe Deng, Linlin Shi, Cailing Fan, Xiufang Xu, Mao-Ping Song
Summary: This study focused on the precise improvement of the photo-isomerized reaction of molecular photoswitches and its importance in the fabrication of high-performance smart materials. The study synthesized a series of stiff-stilbene derivatives and investigated their photoisomerization properties, revealing the influence of electronic effects on the photoisomerization properties. The study also demonstrated the application of these molecular photoswitches in fluorescence behavior and high-secrecy-level anti-counterfeiting.
Article
Hematology
James R. Fuller, Kevin E. Knockenhauer, Nina C. Leksa, Robert T. Peters, Joseph D. Batchelor
Summary: The interaction between FVIII and VWF is crucial for hemostasis, and mutations affecting this interaction can lead to von Willebrand disease type 2N. Understanding the structural basis of this interaction is important for developing therapies for bleeding disorders like hemophilia A. The structure of the bioengineered FVIII molecule BIVV001 provides insights into the FVIII-VWF complex formation and sheds light on mutations associated with hemophilia A and VWD type 2N.
Article
Biotechnology & Applied Microbiology
Jinyang Li, Sheng Tong, Farrukh Raza Amin, Habiba Khalid, Kai Chen, Xiaoguang Zhao, Jinling Cai, Demao Li
Summary: The use of self-inducible promoters is a promising strategy to address metabolic imbalances caused by overexpression. However, the low activity of natural self-inducible promoters hinders their widespread application. To overcome this limitation, a enhanced self-inducible promoter library was created using saturation mutations and high-throughput screening. Sequence analysis revealed certain characteristics common to these promoters, including semi-conservation in the -35 hexamer, highly conserved cytosine in the -17 motif, and moderate A+T content in the spacer region. The identified promoters were used as representatives to measure specific fluorescence and OD600 nm dynamics in different media and confirm their effect on the expression of different proteins, providing valuable guidance for modifying promoters and developing a promoter library suitable for regulating target genes.
FERMENTATION-BASEL
(2023)