Article
Chemistry, Physical
Kelsey A. Parker, Jonathan D. Schultz, Niven Singh, Michael R. Wasielewski, David N. Beratan
Summary: This study investigates the ability of simple feedforward neural networks (NNs) to map simulated 2D electronic spectra to underlying physical Hamiltonians. The results demonstrate that NNs can accurately characterize most Hamiltonian parameters, aiding in interpreting 2D spectra.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Article
Nanoscience & Nanotechnology
Tao Zhang, Hongtai Chen, Linyan Wang, Yurong Liang, Xiutang Zhang
Summary: This study aimed to investigate the sensing performance of a honeycomb nanochannel of heterometallic MOFs with excellent properties. The results showed that the nanochannel had ultrastrong fluorescence quenching for tryptophan and high catalytic activity for a cycloaddition reaction. Therefore, this research demonstrated the potential applications of this nanoporous MOF in clinical monitoring of tryptophan and as a catalyst.
ACS APPLIED NANO MATERIALS
(2022)
Article
Biochemical Research Methods
Mira A. M. Behnam, Thomas Basche, Christian D. P. Klein
Summary: Ligand-protein binding assays based on intrinsic protein fluorescence are straightforward and inexpensive methods. In this paper, the applicability of a FRET-based sensor tag is described, which can be incorporated into high-affinity ligands to generate target-specific compounds that can quench protein fluorescence upon binding.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Yoshiki Inoue, Tomohiro Yamaguchi, Tetsuo Otsuka, Yuto Utsunomiya, Dongqing Pan, Haruo Ogawa, Hiroaki Kato
Summary: This study presents a system for measuring the affinity of substrate binding to CmABCB1 using site-specific tryptophan fluorescence quenching. The results suggest that this system is an ideal method to determine the substrate binding affinities of compounds bound to the chamber of mammalian ABCB1. This research is important for understanding the structure-function relationship of ABCB1.
Article
Nanoscience & Nanotechnology
Imen Boumar, Muhammedin Deliorman, Pavithra Sukumar, Mohammad A. Qasaimeh
Summary: This paper introduces a novel hybrid flow assay for the detection and screening of IgM and IgG antibodies in SARS-CoV-2 infections. The assay utilizes gold nanoparticles conjugated with SARS-CoV-2 S and N proteins to capture antibodies from a small amount of blood in just one step and provides quick results. The adhesive bandage-like design allows for rapid, low-cost, and easy-to-use tests for large-scale detection and screening in households.
MICROSYSTEMS & NANOENGINEERING
(2023)
Article
Chemistry, Applied
Leandro Fabian Bustos, Maria Alicia Judis, Franco Emanuel Vasile, Oscar Edgardo Perez
Summary: This study aims to explore the molecular interactions between buffalo whey proteins and folic acid, and finds that they strongly bind through non-covalent interactions and form complexes. Buffalo whey proteins improve the photostability of folic acid against UV radiation, while chemical denaturation negatively affects the binding properties. The results encourage further research on the application of buffalo whey proteins as carrier agents.
Article
Biochemical Research Methods
Ting Wu, Jiuling Liao, Feng Xiang, Jia Yu, Yufeng Gao, Lina Liu, Shiwei Ye, Hui Li, Kebin Shi, Wei Zheng
Summary: This study developed a special two-photon microscopy system that can measure short-wavelength endogenous fluorophores and provide label-free three-dimensional imaging in vivo or ex vivo. The results showed that the system can noninvasively visualize fine structures and dynamics in biological systems.
BIOMEDICAL OPTICS EXPRESS
(2023)
Article
Materials Science, Multidisciplinary
Yuren Xiang, Yameng Cao, Wenqiang Yang, Rui Hu, Sebastian Wood, Bowei Li, Qin Hu, Fan Zhang, Jujie He, Mozhgan Yavari, Jinlai Zhao, Yunlong Zhao, Jun Song, Junle Qu, Rui Zhu, Thomas P. Russell, S. Ravi P. Silva, Wei Zhang
Summary: A recoverable laser-induced fluorescence quenching phenomenon was observed in perovskite films, indicating the impact of perovskite lattice deformation on transient optical properties. Adjusting charge carrier recombination can improve the bleaching resistance of perovskite grains.
ENERGY & ENVIRONMENTAL MATERIALS
(2022)
Article
Chemistry, Applied
Danilo C. C. Vidotto, Raphaela A. A. Mantovani, Guilherme M. M. Tavares
Summary: Dynamic high-pressure homogenization microfluidization (DHPM) is a versatile emerging technology that can induce changes in protein structure based on the nature of molecules and working parameters. Application of DHPM with cooling device on whey protein isolate results in minor structural changes, while without cooling device, aggregation and increased protein surface hydrophobicity are observed.
Article
Biochemistry & Molecular Biology
Rachana Rao Battaje, Prajakta Bhondwe, Hemendra Pal Singh Dhaked, Dulal Panda
Summary: FtsZ from Streptococcus pneumoniae undergoes a conformational switch around W294 during polymerization, with W294 being more buried in the structure. This region is important for monomer-monomer interaction during protofilament formation.
Article
Chemistry, Physical
Hanaa A. Hassanin
Summary: The interaction between vitamin B-2 and vitamin B-12 in an aqueous solution was investigated using fluorescence quenching and UV-vis absorption spectroscopy. The results showed that the fluorescence intensity of vitamin B-2 was significantly quenched by vitamin B-12 at different pH and temperature conditions. The quenching mechanism was determined to be static quenching, and electron transfer between the two compounds was thermodynamically favored. Distance analysis suggested the possibility of Forster resonance energy transfer between vitamin B-2 and vitamin B-12. The electrostatic interaction was found to play an important role in the reaction between the two compounds.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2022)
Article
Biophysics
Ryosuke Fukushima, Johtaro Yamamoto, Masataka Kinjo
Summary: Number and brightness (N&B) analysis is a useful method for monitoring the spatial distribution of concentration and oligomeric state of fluorescently labeled proteins in cells. The proposed EB-MAP method improves the accuracy and precision in determining particle number and brightness compared with the traditional MoM method, making it valuable for studies on changes in cellular molecular mechanisms.
BIOPHYSICAL JOURNAL
(2021)
Article
Engineering, Environmental
Sanjeeb Mohapatra, Neha Sharma, Gayatree Mohapatra, Lokesh P. Padhye, Suparna Mukherji
Summary: In this study, wastewater samples from five WWTPs in western India were analyzed using fluorescence spectroscopy. The hydrophilic acid and hydrophilic neutral fractions were found to be more abundant than the hydrophilic base fraction in both influent and effluent wastewater streams. Trphtophan-like and tyrosine-like substances were also detected in the wastewater. LC-MS/MS analysis identified hundreds of DOM proteins in the influent and effluent streams, some of which were related to Alzheimer's and Huntington disease, serving as potential biomarkers for wastewater-based epidemiology.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Bintian Zhang, Eathen Ryan, Xu Wang, Stuart Lindsay
Summary: Proteins, as molecular electronic components, have a long electronic decay length of around 10 nm, high contact resistance, and extreme sensitivity to chemical details of the contact. The conductance of even large bioelectronic assemblies is largely controlled by the conductance of the contacts. Using engineered streptavidin tetramers with defined ratios of active to dead monomers, researchers found a strong dependence of conductance on the separation of biotin molecules, indicating a short-range tunneling interaction within streptavidin. The presence of hexaglutamate tails on active monomers enhances conductance significantly through an electronic resonance effect in protein conductance.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Pharmacology & Pharmacy
Jaakko Itkonen, Leo Ghemtio, Daniela Pellegrino, Pia J. Jokela (nee Heinonen), Henri Xhaard, Marco G. Casteleijn
Summary: In this study, time-gated Raman spectroscopy was used to analyze the unfolding of proteins, providing a foundation for predictive modeling of unfolding events during production and storage.