Article
Biochemistry & Molecular Biology
Matthias Ostermeier, Steffen Heinz, Julia Hamm, Jure Zabret, Anna Rast, Andreas Klingl, Marc M. Nowaczyk, Joerg Nickelsen
Summary: Thylakoids are specialized internal membrane systems that house photosynthetic electron transport machinery in cyanobacteria and chloroplasts. The proteins AncM and CurT have antagonistic roles in shaping thylakoid ultrastructure.
Article
Multidisciplinary Sciences
Christopher J. Gisriel, Jimin Wang, Jinchan Liu, David A. Flesher, Krystle M. Reiss, Hao-Li Huang, Ke R. Yang, William H. Armstrong, M. R. Gunner, Victor S. Batista, Richard J. Debus, Gary W. Brudvig
Summary: The cryo-electron microscopy structure of PSII from Synechocystis sp. PCC 6803 has been determined at high resolution, revealing differences from thermophilic cyanobacterial PSII structures that significantly impact the understanding of PSII structure and the mechanism of water oxidation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Rui Miao, Michael Jahn, Kiyan Shabestary, Gilles Peltier, Elton P. Hudson
Summary: Barcoded mutant libraries are a powerful tool for studying gene function in microbes. In this study, a CRISPR interference library of the cyanobacterium Synechocystis sp. PCC 6803 was screened in 11 different growth conditions, revealing condition-specific gene fitness. The data set provides new insights into gene regulation and identifies potential functions for previously unannotated genes and central metabolic enzymes. Machine learning was used to predict the potency of sgRNA sequences, showing the importance of specific nucleotide patterns. The results of this study contribute to our understanding of microbial gene function and regulation.
Article
Multidisciplinary Sciences
Shoko Kusama, Seiji Kojima, Ken Kimura, Ginga Shimakawa, Chikahiro Miyake, Kenya Tanaka, Yasuaki Okumura, Shuji Nakanishi
Summary: This study reports a significant enhancement in the photocurrent generation of cyanobacteria by depriving them of their outer cell membrane. These findings are important for overcoming the limitations of cyanobacteria in biophotovoltaic applications.
NATURE COMMUNICATIONS
(2022)
Article
Plant Sciences
Sandeesha Kodru, Laszlo Sass, Priyanka Patil, Milan Szabo, Imre Vass
Summary: In this study, a TL component similar to the plant AG band, originating from NDH-1-mediated CEF, was identified in cyanobacteria. The +40 degrees C band was most efficiently induced by FR illumination at -10 degrees C, and it was observed that low temperatures block CEF at two different sites in Synechocystis PCC 6803.
PHYSIOLOGIA PLANTARUM
(2021)
Article
Biochemistry & Molecular Biology
Jana Knoppova, Jianfeng Yu, Jan Janouskovec, Petr Halada, Peter J. Nixon, Julian P. Whitelegge, Josef Komenda
Summary: Photochemical energy conversion in oxygenic photosynthesis relies on membrane-embedded chlorophyll-binding protein complexes, which are regulated by lipoproteins such as Ycf48. Ycf48, a lipoprotein assembly factor in cyanobacteria, plays a crucial role in the biogenesis and function of the photosystem II complex. The unique lipobox sequence in Ycf48 distinguishes it from canonical lipobox sequences and highlights its importance in cyanobacterial PSII assembly.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Shoko Kusama, Chikahiro Miyake, Shuji Nakanishi, Ginga Shimakawa
Summary: Cyclic electron transport (CET) is a hypothesis for regulating photosynthetic electron transport and producing additional ATP, with its activity in cyanobacteria being evaluated through P700 redox state and chlorophyll fluorescence. In cyanobacteria, distinguishing between in vivo electron transport rates in respiratory electron transport (RET) and CET can be complex due to shared intersystem chains.
JOURNAL OF PLANT RESEARCH
(2022)
Article
Plant Sciences
David Fuente, Dusan Lazar, Jose Vicente Oliver-Villanueva, Javier F. Urchueguia
Summary: The study reconstructed the absorption spectrum of different strains by summing the computed signature of all pigments present in the organism. Modifications to in vitro pigment spectra were made and a plausible shape for the in vivo absorption spectrum of each chromophore was outlined. The study also provided estimates of pigment concentrations without relying on spectrophotometric correlations, and accurately reproduced experimental spectra for studied wavelengths in various strains.
PHOTOSYNTHESIS RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Kateryna Kukil, Elias Englund, Nick Crang, Elton P. Hudson, Pia Lindberg
Summary: In this study, phenylalanine resistant mutant strains of the unicellular cyanobacterium Synechocystis sp. PCC 6803 were generated by laboratory evolution. The mutant strains were able to secrete phenylalanine and showed potential for production of trans-cinnamic acid and para-coumaric acid. The PRM8 strain demonstrated the highest specific production of these compounds.
METABOLIC ENGINEERING
(2023)
Article
Plant Sciences
Henna Mustila, Dorota Muth-Pawlak, Eva-Mari Aro, Yagut Allahverdiyeva
Summary: In this study, the global proteome of Synechocystis sp. PCC 6803 under fluctuating light conditions was compared with constant light conditions. It was found that cell metabolism was clearly reprogrammed, and there were correlations between mRNA and protein levels to some extent. The data suggest that higher nitrogen assimilation is maintained for long-term acclimation to fluctuating light upon CO2 step-down in Synechocystis sp. PCC 6803.
PHYSIOLOGIA PLANTARUM
(2021)
Article
Plant Sciences
Pornpan Napaumpaiporn, Takako Ogawa, Kintake Sonoike, Yoshitaka Nishiyama
Summary: This study demonstrates that overexpression of superoxide dismutase and catalase in cyanobacteria can reduce ROS accumulation and enhance the repair of photosystem II (PSII), enabling photosynthesis and cell proliferation to adapt to strong light conditions.
Correction
Chemistry, Multidisciplinary
Sharon L. Smolinski, Carolyn E. Lubner, Zhanjun Guo, Jacob H. Artz, Katherine A. Brown, David W. Mulder, Paul W. King
Summary: This correction article by Sharon L. Smolinski et al. focuses on the influence of electron utilization pathways on photosystem I photochemistry in Synechocystis sp. PCC 6803. The study reveals that different electron utilization pathways have distinct effects on the photochemical process of photosystem I, which is of great significance for further understanding the mechanism of photosynthesis.
Article
Biochemistry & Molecular Biology
Elena A. Protasova, Taras K. Antal, Dmitry Zlenko, Irina Elanskaya, Evgeny P. Lukashev, Thomas Friedrich, Kirill S. Mironov, Nikolai N. Sluchanko, Baosheng Ge, Song Qin, Eugene G. Maksimov
Summary: The quenching of excess excitation energy is crucial for photoprotection of light-harvesting complexes, especially in cyanobacteria where the Orange Carotenoid Protein (OCP) induces quenching of phycobilisome (PBS) excitation energy. OCP-dependent quenching affects approximately half of the PBSs coupled to PS II, suggesting that the concentration of OCP may not be sufficient for quenching all PBSs under normal conditions.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2021)
Article
Genetics & Heredity
Chen Xu, Bing Wang, Lin Yang, Lucas Zhongming Hu, Lanxing Yi, Yaxuan Wang, Shenglan Chen, Andrew Emili, Cuihong Wan
Summary: In this study, using co-fractionation coupled with LC-MS/MS, the researchers identified 291 multi-protein complexes and 24,092 protein-protein interactions in Synechocystis sp. PCC 6803. These interactions reveal the roles of photosynthesis in various physiological processes and highlight the differences in protein functions between bacteria and higher plants.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2022)
Article
Biochemistry & Molecular Biology
Daniel Solymosi, Dmitry Shevela, Yagut Allahverdiyeva
Summary: The study demonstrates that the gaseous radical nitric oxide (NO) can block photosynthetic electron transfer in cyanobacterial cells by repressing PSII, PSI, and likely the NDH-1 complex. These findings reveal the profound effects of NO on cyanobacterial cells and emphasize the importance of controlled NO homeostasis in cyanobacteria.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2022)
Article
Biochemistry & Molecular Biology
David J. K. Swainsbury, Kaitlyn M. Faries, Dariusz M. Niedzwiedzki, Elizabeth C. Martin, Adam J. Flinders, Daniel P. Canniffe, Gaozhong Shen, Donald A. Bryant, Christine Kirrnaier, Dewey Holten, C. Neil Hunter
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2019)
Article
Plant Sciences
Gaozhong Shen, Daniel P. Canniffe, Ming-Yang Ho, Vasily Kurashov, Art van der Est, John H. Golbeck, Donald A. Bryant
PHOTOSYNTHESIS RESEARCH
(2019)
Article
Plant Sciences
Vasily Kurashov, Ming-Yang Ho, Gaozhong Shen, Karla Piedl, Tatiana N. Laremore, Donald A. Bryant, John H. Golbeck
PHOTOSYNTHESIS RESEARCH
(2019)
Correction
Microbiology
Chi Zhao, Fei Gan, Gaozhong Shen, Donald A. Bryant
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Plant Sciences
Donald A. Bryant, Gaozhong Shen, Gavin M. Turner, Nathan Soulier, Tatiana N. Laremore, Ming-Yang Ho
PHOTOSYNTHESIS RESEARCH
(2020)
Article
Multidisciplinary Sciences
Christopher Gisriel, Gaozhong Shen, Vasily Kurashov, Ming-Yang Ho, Shangji Zhang, Dewight Williams, John H. Golbeck, Petra Fromme, Donald A. Bryant
Article
Biochemistry & Molecular Biology
Dmitry A. Cherepanov, Ivan Shelaev, Fedor E. Gostev, Arseniy Aybush, Mahir D. Mamedov, Gaozhong Shen, Victor A. Nadtochenko, Donald A. Bryant, Alexey Yu Semenov, John H. Golbeck
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2020)
Article
Biochemistry & Molecular Biology
Martijn Tros, Luca Bersanini, Gaozhong Shen, Ming-Yang Ho, Ivo H. M. van Stokkum, Donald A. Bryant, Roberta Croce
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2020)
Article
Multidisciplinary Sciences
Xingqian Cui, Xiao-Lei Liu, Gaozhong Shen, Jian Ma, Fatima Husain, Donald Rocher, John E. Zumberge, Donald A. Bryant, Roger E. Summons
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Chemistry, Multidisciplinary
Martijn Tros, Vincenzo Mascoli, Gaozhong Shen, Ming-Yang Ho, Luca Bersanini, Christopher J. Gisriel, Donald A. Bryant, Roberta Croce
Summary: Chlorophyll f helps cyanobacteria absorb low-energy photons, but its energetic connectivity with other pigments is not optimal. Despite this, chlorophyll f still consistently traps long-wavelength excitations with high efficiency, which may be achieved by lowering the energy required for photochemistry.
Article
Biochemistry & Molecular Biology
Christopher J. Gisriel, Gaozhong Shen, Ming-Yang Ho, Vasily Kurashov, David A. Flesher, Jimin Wang, William H. Armstrong, John H. Golbeck, Marilyn R. Gunner, David J. Vinyard, Richard J. Debus, Gary W. Brudvig, Donald A. Bryant
Summary: Far-red light photoacclimation expands the range of photosynthetically active radiation for terrestrial cyanobacteria, providing a growth advantage. This process modifies the structure of photosystem II and lowers the energy required for water oxidation.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Christopher J. Gisriel, David A. Flesher, Gaozhong Shen, Jimin Wang, Ming-Yang Ho, Gary W. Brudvig, Donald A. Bryant
Summary: Some cyanobacteria can use far-red light for photosynthesis. The study identified the specific binding sites of chlorophyll f in photosystem I and revealed structural differences in far-red light-acclimated cyanobacteria.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Plant Sciences
Nathan Soulier, Karim Walters, Tatiana N. Laremore, Gaozhong Shen, John H. Golbeck, Donald A. Bryant
Summary: Based on their growth responses to high and low irradiance, thermophilic Synechococcus sp. isolates from Mushroom Spring in Yellowstone National Park can be classified as high-light or low-light ecotypes. The low-light ecotypes show increased growth at low irradiance and develop far-red absorbance and fluorescence after growth in low light. These ecotypes have unique gene clusters encoding cyanobacteriochrome, sensor histidine kinase, far-red light absorbing allophycocyanin, and a chlorophyll a-binding protein. The emergence of far-red absorbance in low-light adapted cells is a result of a novel antenna complex containing far-red light absorbing allophycocyanin. Additionally, the two GAF domains of the cyanobacteriochrome LcyA bind different ligands that potentially enable this photoreceptor to respond to various environmental factors.
PHOTOSYNTHESIS RESEARCH
(2022)
Article
Microbiology
Bayleigh Murray, Emine Ertekin, Micah Dailey, Nathan T. Soulier, Gaozhong Shen, Donald A. Bryant, Cesar Perez-Fernandez, Jocelyne DiRuggiero
Summary: In hyper-arid deserts, endolithic cyanobacteria adapt to the far-red light environment by synthesizing chlorophyll f. Through Far-Red-Light Photoacclimation (FaRLiP) response, the cyanobacteria are able to adjust their photosynthetic apparatus and modify their light-harvesting complexes.
Article
Multidisciplinary Sciences
Craig MacGregor-Chatwin, Dennis J. Nurnberg, Philip J. Jackson, Cvetelin Vasilev, Andrew Hitchcock, Ming-Yang Ho, Gaozhong Shen, Christopher J. Gisriel, William H. J. Wood, Moontaha Mahbub, Vera M. Selinger, Matthew P. Johnson, Mark J. Dickman, Alfred William Rutherford, Donald A. Bryant, C. Neil Hunter
Summary: Cyanobacteria have developed strategies to adapt to different environments and utilize various strategies for photosynthesis. The study characterizes the molecular changes involved in FaRLiP and the supramolecular organization of photosystem I under FRL light, showing altered cellular distribution.