Article
Biochemistry & Molecular Biology
Catherine Duport, Jean-Paul Madeira, Mahsa Farjad, Beatrice Alpha-Bazin, Jean Armengaud
Summary: The study reveals that under fermentative anoxic conditions, MsrA plays a significant role in antioxidant activities, affecting cell growth, motility, and energy metabolism.
Review
Biochemistry & Molecular Biology
Lionel Tarrago, Alaattin Kaya, Hwa-Young Kim, Bruno Manta, Byung-Cheon Lee, Vadim N. Gladyshev
Summary: Methionine sulfoxide reductases (MSR) are enzymes that reduce methionine sulfoxide (MetO) back to methionine. MSRB1, a selenoprotein found in mammals, uses selenocysteine as a catalytic residue. It plays a crucial role in oxidative stress protection, neuronal cell maintenance, cognition, cancer cell proliferation, and immune response.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Hayat Hage, Marie-Noelle Rosso, Lionel Tarrago
Summary: Research shows that most fungi possess genes for three types of methionine sulfoxide reductase: MsrA, MsrB, and fRMsr in their genomes. However, some fungi living in anaerobic environments or as obligate intracellular parasites lack these genes. Additionally, several horizontal gene transfer events from bacteria to fungi involving msr genes have been identified.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Jana Schepers, Zorana Carter, Paraskevi Kritsiligkou, Chris M. Grant
Summary: This study reveals that methionine oxidation is a key factor in the formation of the yeast [PSI+] prion. By lacking methionine sulfoxide reductases, it is found that [PSI+] formation is increased, while lacking both isoenzymes of methionine sulfoxide reductases reduces [PSI+] formation.
Article
Chemistry, Multidisciplinary
Hae Min Lee, Dong Wook Choi, Seahyun Kim, Aro Lee, Minseo Kim, Yeon Jin Roh, Young Ho Jo, Hwa Yeon Cho, Ho-Jae Lee, Seung-Rock Lee, Lionel Tarrago, Vadim N. Gladyshev, Ji Hyung Kim, Byung Cheon Lee
Summary: This study developed a biological sensor, tpMetROG, for quantifying oxidized methionine in target proteins, demonstrating its versatile application in various proteins.
Article
Chemistry, Physical
Silvia Anselmi, Alexandra T. P. Carvalho, Angela Serrano-Sanchez, Jose L. Ortega-Roldan, Jill Caswell, Iman Omar, Gustavo Perez-Ortiz, Sarah M. Barry, Thomas S. Moody, Daniele Castagnolo
Summary: This study identifies selective and robust MsrA biocatalysts capable of efficiently catalyzing enantioselective reduction of various aromatic and aliphatic chiral sulfoxides at concentrations of 8-64 mM with high yields and excellent enantiomeric excess (up to 99%). Through rational mutagenesis design using in silico docking, molecular dynamics, and structural nuclear magnetic resonance (NMR) studies, a mutant enzyme MsrA33 was developed, which can catalyze the kinetic resolution of bulky sulfoxide substrates with non-methyl substituents on the sulfur atom with enantiomeric excess up to 99%, overcoming a significant limitation of current MsrA biocatalysts.
Review
Chemistry, Multidisciplinary
Tao Peng, Xiaoling Cheng, Yongzheng Chen, Jiawei Yang
Summary: Chiral sulfoxides, valuable organosulfur compounds widely used in medicinal and organic synthesis, can be prepared through biocatalytic approaches, with sulfoxide reductases emerging as a new method with extraordinary catalytic properties. This review article discusses the chemical and biological functions of sulfoxide reductases and highlights their applications in chiral sulfoxide preparation.
FRONTIERS IN CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Tao Peng, Jin Tian, Yuyan Zhao, Xu Jiang, Xiaoling Cheng, Guozhong Deng, Quan Zhang, Zhongqiang Wang, Jiawei Yang, Yongzheng Chen
Summary: This study achieved the cyclic deracemization of sulfoxides by combining the high-enantioselective enzyme MsrA with the low-enantioselective enzyme SMO, resulting in enantiopure sulfoxides. The cofactors of the enzymes were regenerated by the cascade catalysis of auxiliary enzymes, enabling high yield and enantioselectivity.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Review
Biochemistry & Molecular Biology
Jackob Moskovitz, Adam Smith
Summary: Methionine oxidation and reduction commonly occur in biological systems, with levels of MetO dependent on the redox status impacting protein function and toxicity accumulation. The Msr system plays a crucial role in regulating the effects of MetO.
Article
Biochemistry & Molecular Biology
Marufa Nasreen, Remya Purushothaman Nair, Alastair G. McEwan, Ulrike Kappler
Summary: Sulfoxide-damage repair mechanisms are crucial for the virulence of bacterial pathogens. The periplasmic protein MsrAB in the human respiratory pathogen Haemophilus influenzae plays a key role in resisting reactive chlorine species. It also modulates the host immune response. Our study reveals the enzymatic properties of MsrAB, its catalytic activity, and potential substrates. This research sheds light on the maintenance of adhesins and essential metabolic processes in H. influenzae.
Article
Biochemistry & Molecular Biology
Arianna Luise, Elena De Cecco, Erika Ponzini, Martina Sollazzo, PierLuigi Mauri, Frank Sobott, Giuseppe Legname, Rita Grandori, Carlo Santambrogio
Summary: Post-translational modifications can lead to diverse functional roles for a single protein, with oxidation being a common modification. Analyzing the interaction of proteins with dopamine and characterizing the proteoforms generated by dopamine-induced oxidation through top-down fragmentation can provide insights into the structural changes and relative abundance of different protein states.
Article
Biochemistry & Molecular Biology
Lihue N. Gonzalez, Matias S. Cabeza, Carlos Robello, Sergio A. Guerrero, Alberto A. Iglesias, Diego G. Arias
Summary: Trypanosoma cruzi is a unicellular parasite that causes Chagas Disease and infects various mammalian hosts. It exhibits auxotrophy for L-Met and requires the amino acid from the extracellular environment of the host. The study identified a coding sequence for a free-R-MSR enzyme in T. cruzi, which is involved in the reduction of L-MetSO using specific cysteine residues. The results provide new insights into the redox metabolism of T. cruzi and contribute to understanding L-Met metabolism in this parasite.
Article
Biochemistry & Molecular Biology
Laurent Loiseau, Alexandra Vergnes, Benjamin Ezraty
Summary: Repairing oxidative-targeted macromolecules is essential for organisms to adapt to oxidative stress. In Escherichia coli, contamination by chlorate led to the high expression of msrPQ and methionine oxidation. This study challenges the traditional view of the absence of methionine oxidation during anaerobiosis.
MOLECULAR MICROBIOLOGY
(2022)
Article
Plant Sciences
Li Wang, Jieyu Yang, Wenjun Tan, Yile Guo, Jiaqi Li, Chuntao Duan, Gehong Wei, Minxia Chou
Summary: In the symbiosis of M. truncatula-Sinorhizobium meliloti, MtMIF3 is mainly expressed in the nitrogen-fixing zone of the nodules. Silencing MtMIF3 increased nodule numbers but resulted in higher bacteroid degradation in the infected cells of the nitrogen-fixing zone, indicating premature aging. On the other hand, overexpression of MtMIF3 inhibited nodule senescence.
PLANT CELL AND ENVIRONMENT
(2023)
Review
Biochemistry & Molecular Biology
Maxence S. Vincent, Benjamin Ezraty
Summary: Methionine, a sulfur-containing residue in proteins, is prone to oxidation which can damage or activate protein function. Many bacteria possess enzymatic systems to reduce oxidized methionine, highlighting its reversible post-translational modification role. This review examines recent examples of methionine oxidation leading to protein loss or gain of function, and speculates on its role as a multilayer endogenous antioxidant system and its potential consequences for bacterial virulence.
MOLECULAR MICROBIOLOGY
(2022)
Review
Oncology
Sarmad Sheraz Jadoon, Umair Ilyas, Hajra Zafar, Ana Claudia Paiva-Santos, Saifullah Khan, Saeed Ahmad Khan, Tanzeel Ahmed, Yasir Rasool, Reem Altaf, Faisal Raza, Muhammad Abbas
Summary: This article discusses the severity of glioblastoma multiforme and the shortcomings of current treatment methods. It emphasizes the importance of using novel biomarkers for diagnosis and prognosis, and also explores recent clinical advancements and potential therapeutic methods.
JOURNAL OF ONCOLOGY
(2022)
Article
Oncology
Adeel Khan, Irfan Tariq, Haroon Khan, Sifat Ullah Khan, Nongyue He, Li Zhiyang, Faisal Raza
Summary: This study proposes an innovative strategy called AdaBoost-SNMV-CNN for detecting lung cancer nodules in CT scans. The method achieves high accuracy and sensitivity, outperforming other models. The model has good generalization and learning ability, a simple architecture, and a short computational time, making it a potential tool for noninvasive clinical diagnosis of lung cancer.
JOURNAL OF ONCOLOGY
(2022)
Article
Pharmacology & Pharmacy
Zhaoming Ma, Xuzhu Gao, Faisal Raza, Hajra Zafar, Guanhong Huang, Yunyun Yang, Feng Shi, Deqiang Wang, Xia He
Summary: The developed nanomicelles Cur@ATRA-SS-HA in this study showed excellent targeting and stable release behavior, providing a novel clinical treatment for oesophageal cancer through effective release of curcumin in the tumor microenvironment.
Article
Materials Science, Biomaterials
Mahira Zeeshan, Qurat Ul Ain, Ahad Sunny, Faisal Raza, Muhammad Mohsin, Salman Khan, Benno Weigmann, Hussain Ali
Summary: Colon mucosal inflammation attracts immune cells with overexpressed surface receptors. By exploiting these receptors, Tofa-P/tfr NCs were developed for specific binding to transferrin receptors on macrophages and colon epithelial cells. These nanocarriers showed desired properties and demonstrated good colon targeting potential in an ulcerative colitis model. In vitro and in vivo studies confirmed their biocompatibility, uptake, and therapeutic efficacy in mitigating mucosal inflammation.
BIOMATERIALS SCIENCE
(2023)
Review
Pharmacology & Pharmacy
Arbab Ali, Rabia Javed, Sahar Farhangi, Tufail Shah, Sana Ullah, Noor ul Ain, Tao Liu, Iseult Lynch, Faisal Raza, Peng Zhang, YuKui Rui
Summary: The tumor microenvironment is a complex system in which conventional drug delivery strategies have limitations. The development of nano-based drug delivery has provided solutions by offering controlled release in this environment. This review focuses on the use of metal phenolic networks as pH-sensitive drug carriers in the tumor microenvironment, providing insights and exploring potential applications.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Genetics & Heredity
Zhiwei Miao, Mingjia Gu, Faisal Raza, Hajra Zafar, Jianyi Huang, Yuhang Yang, Muhammad Sulaiman, Jing Yan, Yi Xu
Summary: This study confirmed the anti-inflammatory effect of isoliquiritin on rats with TNBS-induced colitis, and explored its underlying mechanism. The results showed that isoliquiritin could alleviate intestinal inflammation and mucosal damage through regulating the caspase-3/HMGB1/TLR4 signaling pathway.
CURRENT GENE THERAPY
(2023)
Article
Materials Science, Biomaterials
Liangdi Jiang, Xuerui Wang, Faisal Raza, Hongyu Zhong, Jing Su, Wei-En Yuan, Mingfeng Qiu
Summary: In this study, a pH-responsive nanoplatform (PEG-MSN@ATO) was developed to enhance the therapeutic efficacy of arsenic trioxide (ATO) against liver cancer (LC). PEG-MSN@ATO induced apoptosis and ROS production, inhibited tumor cell proliferation and metastasis, and activated antitumor immunity within the tumor microenvironment (TME). The nanoplatform reduced the toxicity of ATO and improved its therapeutic effectiveness.
BIOMATERIALS SCIENCE
(2023)
Article
Oncology
Yufang Chen, Qianqian Gao, Faisal Raza, Hajra Zafar, Ziyan Li, Jingyao Zeng, Meihua Wang, Pan Huang, Jianhua Su
Summary: This study found the expressions of CK, CTSD, Ki67, and p63 in ESCC and their association with the differentiation of ESCC. Specifically, CTSD, p63, and Ki67 were highly expressed in poorly differentiated patients. These findings suggest the involvement of these proteins in ESCC progression and their potential as markers for early identification and risk assessment of ESCC.
EUROPEAN JOURNAL OF CANCER CARE
(2023)
Article
Pharmacology & Pharmacy
Junhua Zhang, Rui Bao, Faisal Raza, Hajra Zafar, Yingjie Qi, Yurui Geng, Ran Li, Jianqin Xue, Feng Shi
Summary: The study found that loaded OB in liposomes has therapeutic effects on Parkinson's disease models, improving motor deficits and reducing dopaminergic neuron loss. Furthermore, lip OB improves mitochondrial dysfunction in PD models by promoting mitophagy. This provides a new possibility for treating PD.
JOURNAL OF CLINICAL PHARMACY AND THERAPEUTICS
(2023)
Review
Materials Science, Biomaterials
Faisal Raza, Hajra Zafar, Liangdi Jiang, Shulei Zhang, Jing Su, Weien Yuan, Mingfeng Qiu, Ana Claudia Paiva-Santos
Summary: This article provides a comprehensive overview of the application of cell membrane-derived biomimetic nanovesicles in cancer phototherapy, as well as discussing the challenges and future prospects for clinical translation.
BIOMATERIALS SCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Sara Cabanas Coimbra, Ines Sousa-Oliveira, Ines Ferreira-Faria, Diana Peixoto, Miguel Pereira-Silva, Ankita Mathur, Kiran D. Pawar, Faisal Raza, Priscila Gava Mazzola, Filipa Mascarenhas-Melo, Francisco Veiga, Ana Claudia Paiva-Santos
Summary: This review article provides an overview of the use and safety assessment of nanomaterials in cosmetics. It emphasizes the insufficient safety assessment in the field and the need for more research on the effects of nanosized particles on living systems, particularly considering the complex interactions between biological systems and active cosmetic ingredients.
Article
Pharmacology & Pharmacy
Mensura Sied Filli, Ahmed Abdalla Ibrahim, Samuel Kesse, Md Aquib, Kofi Oti Boakye-Yiadom, Muhammad Asim Farooq, Faisal Raza, Yubin Zhang, Bo Wang
Summary: The process of new drug discovery and development is both exciting and challenging. Natural products, such as berberine, have gained attention as potential sources for new chemical entities. However, barriers such as toxicological and pharmacokinetic properties hinder the development of berberine into a marketed drug product. Synthetic berberine derivatives are suggested as better alternatives for further development. Collaboration among scientists is essential to exploit the value of natural products for effective drug development.
BRAZILIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2022)
Article
Materials Science, Biomaterials
Aftab Ullah, Gang Chen, Zhang Yibang, Abid Hussain, Muhammad Shafiq, Faisal Raza, Daojun Liu, Kaikai Wang, Jin Cao, Xueyong Qi
Summary: This study proposes a CXCR4-targeted combination liposomal therapy for treating CCl4-induced liver fibrosis. The results demonstrate that this therapy shows good drug delivery, targeted action, and antifibrotic effects, providing a new alternative for antifibrotic treatment.
BIOMATERIALS SCIENCE
(2022)
Review
Materials Science, Biomaterials
Chunxia Liu, Faisal Raza, Hai Qian, Xin Tian
Summary: Poly(ionic liquid)s (PILs), with their excellent physicochemical properties, have been widely used in the biomedical field, making significant contributions to the advancement of science and technology.
BIOMATERIALS SCIENCE
(2022)
Review
Materials Science, Multidisciplinary
Faisal Raza, Hajra Zafar, Muhammad Wasim Khan, Aftab Ullah, Asif Ullah Khan, Abdul Baseer, Rameesha Fareed, Muhammad Sohail
Summary: The review emphasizes the importance of paclitaxel in cancer treatment, and discusses its application in the field of nanomedicine for cancer treatment. Additionally, various forms of nanomedicine of paclitaxel and future challenges are also discussed.
MATERIALS ADVANCES
(2022)