Journal
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
Volume 26, Issue 5, Pages 322-337Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09205063.2015.1008882
Keywords
nerve growth factor (NGF); nerve regeneration; neurogenic differentiation; basic fibroblast growth factor (bFGF); microsphere
Funding
- Korea Ministry of Health and Welfare [A120357, HI14C0522]
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Growth factors (GFs) (basic fibroblast growth factor (bFGF) and/or nerve growth factor (NGF))-immobilized polycaprolactone (PCL)/Pluronic F127 microspheres were prepared using an isolated particulate-melting method and the sequential binding of heparin and GFs onto the microspheres. The GFs immobilized on the microspheres were released in a sustained manner over 28days, regardless of GF type. From the in vitro culture of muscle-derived stem cells, it was observed that the NGF-immobilized microspheres induced more neurogenic differentiation than the bFGF-immobilized microspheres, as evidenced by a quantitative real-time polymerase chain reaction using specific neurogenic markers (Nestin, GFAP, beta-tubulin, and MAP2) and Western blot (Nestin and beta-tubulin) analyses. The dual bFGF/NGF-immobilized microspheres showed better neurogenic differentiation than the microspheres immobilized with single bFGF or NGF. From the preliminary animal study, the dual bFGF/NGF-immobilized microsphere group also showed effective nerve regeneration, as evaluated by immunocytochemistry using a marker - beta-tubulin. The dual bFGF/NGF-immobilized PCL/Pluronic F127 microspheres may be a promising candidate for nerve regeneration in certain target tissues (i.e. muscles) leading to sufficient reinnervation.
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