Article
Cell Biology
Jia Shi, Yi Yang, Ya-Nan Wang, Qing Li, Xue Xing, An-Ying Cheng, Xiao-Na Zhan, Jie Li, Gang Xu, Fan He
Summary: This study reveals the association between metabolism and the progression of vascular calcification in chronic kidney disease (CKD). High phosphate and uremic serum enhance mitochondrial respiration capacity in vascular smooth muscle cells (VSMCs), while glycolysis level remains unchanged. Suppression of cytochrome c oxidase (COX) activity attenuates vascular calcification.
CELL DEATH & DISEASE
(2022)
Article
Multidisciplinary Sciences
Gizem Inak, Agnieszka Rybak-Wolf, Pawel Lisowski, Tancredi M. Pentimalli, Rene Juettner, Petar Glazar, Karan Uppal, Emanuela Bottani, Dario Brunetti, Christopher Secker, Annika Zink, David Meierhofer, Marie-Therese Henke, Monishita Dey, Ummi Ciptasari, Barbara Mlody, Tobias Hahn, Maria Berruezo-Llacuna, Nikos Karaiskos, Michela Di Virgilio, Johannes A. Mayr, Saskia B. Wortmann, Josef Priller, Michael Gotthardt, Dean P. Jones, Ertan Mayatepek, Werner Stenzel, Sebastian Diecke, Ralf Kuehn, Erich E. Wanker, Nikolaus Rajewsky, Markus Schuelke, Alessandro Prigione
Summary: Leigh syndrome (LS) is a severe neurometabolic disorder lacking effective models. This study found that LS carrying mutations in SURF1 gene exhibit impaired neuronal morphogenesis due to metabolic deficiencies.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Sibiao Yue, Lei Wang, George N. DeMartino, FangZhou Zhao, Yi Liu, Matthew H. Sieber
Summary: The regulation of cellular proteostasis is connected to the metabolic state of quiescent cells in vivo. The authors demonstrate that shifts in the ubiquitin-proteome system are linked to mitochondrial metabolic changes and respiratory quiescence. Cellular proteostasis defects and mitochondrial dysfunction play a role in infertility, cancer, and age-related diseases. Through a multi-organism approach, it is shown that dynamic shifts in the ubiquitin proteasome system drive mitochondrial remodeling during cellular quiescence.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Yuya Nishida, Sachiko Yanagisawa, Rikuri Morita, Hideki Shigematsu, Kyoko Shinzawa-Itoh, Hitomi Yuki, Satoshi Ogasawara, Ken Shimuta, Takashi Iwamoto, Chisa Nakabayashi, Waka Matsumura, Hisakazu Kato, Chai Gopalasingam, Takemasa Nagao, Tasneem Qaqorh, Yusuke Takahashi, Satoru Yamazaki, Katsumasa Kamiya, Ryuhei Harada, Nobuhiro Mizuno, Hideyuki Takahashi, Yukihiro Akeda, Makoto Ohnishi, Yoshikazu Ishii, Takashi Kumasaka, Takeshi Murata, Kazumasa Muramoto, Takehiko Tosha, Yoshitsugu Shiro, Teruki Honma, Yasuteru Shigeta, Minoru Kubo, Seiji Takashima, Yasunori Shintani
Summary: Antimicrobial resistance is a global health problem and there is a rise in drug-resistant species. Our research has identified a new antibiotic compound that can combat drug-resistant Neisseria gonorrhoeae, and our findings on the inhibitory mechanism provide new avenues for modulating protein functions and overcoming antimicrobial resistance.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Michael B. Powner, Glen Jeffery
Summary: Systemic glucose levels can be modulated through specific solar wavelengths that affect mitochondrial metabolism. Different wavelengths of light have opposing effects on mitochondrial respiration, with longer wavelengths increasing ATP production and shorter wavelengths suppressing metabolism. This study in a bumblebee model found that manipulating mitochondrial activity with specific wavelengths of light significantly altered circulating glucose concentration. This research contributes to our understanding of how light can affect systemic metabolism.
Article
Cell Biology
Sue Ellen Brand, Martha Scharlau, Lois Geren, Marissa Hendrix, Clayre Parson, Tyler Elmendorf, Earl Neel, Kaila Pianalto, Jennifer Silva-Nash, Bill Durham, Francis Millett
Summary: Cytochrome c (Cc) has undergone accelerated evolution from anthropoid primates to humans, with several amino acid changes occurring near the binding site of the Cc:CcO complex. These changes affect the dissociation constant and the role of phosphorylation in regulating mitochondrial electron transport and membrane potential.
Article
Biochemistry & Molecular Biology
Andre Fischer, Martin Smiesko
Summary: This study investigates an allosteric site in Cytochrome P450 enzymes, finding it to be well conserved in the CYP2 family with the exception of CYP2E1, and observing an enlargement of ligand tunnels in certain members that facilitate ligand translocation. The research contributes to understanding ligand access phenomena and aids in estimating the selectivity of metabolic transformations using computational techniques.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Elise D. Rivett, Hannah G. Addis, Jonathan V. Dietz, Jayda A. Carroll-Deaton, Shipra Gupta, Koji L. Foreman, Minh Anh Dang, Jennifer L. Fox, Oleh Khalimonchuk, Eric L. Hegg
Summary: In aerobic respiration, heme a is required for the final step catalyzed by aa3-type cytochrome c oxidase. The conversion of heme b to heme o and ultimately to heme a is achieved through two modifications, with the conversion of a methyl group to an aldehyde being catalyzed by heme a synthase (HAS). Previous studies have shown that HAS contains two heme-binding sites, with one site occupied by heme b. However, the function of the other site is still unknown.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Chemistry, Physical
Margareta R. A. Blomberg
Summary: Improvements in density functional theory methodology have made it a powerful tool in studying enzymatic reaction mechanisms, but concerns remain about the reliability of results for metalloenzymes. By using different DFT functionals and combining with experimental data, stable qualitative pictures of reaction mechanisms were obtained.
JOURNAL OF CHEMICAL PHYSICS
(2021)
Article
Multidisciplinary Sciences
Agnes Moe, Justin Di Trani, John L. Rubinstein, Peter Brzezinski
Summary: Energy conversion in aerobic organisms involves electron transfer from low-potential donors to dioxygen through the membrane-bound respiratory chain. The formation of supercomplex CIII2CIV1/2 facilitates electron transfer by two-dimensional diffusion of cyt. c, increasing QH(2):O-2 oxidoreductase activity and suggesting a regulatory role for supercomplex formation in the respiratory chain.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Medicinal
Namita Dube, Sabab Hasan Khan, Riley Sasse, C. Denise Okafor
Summary: Allosteric pathways in proteins involve networks of amino acid residues that transmit signals between distant sites. This study uses molecular dynamics simulations and suboptimal path analysis to discover new allosteric networks in steroid receptors, focusing on evolutionarily conserved pathways. The researchers identify a conserved network connecting a predicted allosteric site between helices 9 and 10 of the ligand-binding domain to the activation function surface 2 (AF-2). The study suggests the evolutionary importance of this site in steroid receptors and provides an approach to discover previously unknown allosteric sites in proteins.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2023)
Article
Chemistry, Medicinal
Namita Dube, Sabab Hasan Khan, Riley Sasse, C. Denise Okafor
Summary: Allosteric pathways in proteins allow for the transmission of signals between distant sites through inter-residue interactions. This study utilizes molecular dynamics simulations and suboptimal path analysis to identify new allosteric networks in steroid receptors, focusing on evolutionarily conserved pathways. The investigation reveals a conserved network connecting a predicted allosteric site located between helices 9 and 10 of the ligand-binding domain to the activation function surface 2 (AF-2). The importance of this site is highlighted by its presence in a region characterized by conserved inter-residue contacts.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2023)
Article
Multidisciplinary Sciences
Luke E. Formosa, Shadi Maghool, Alice J. Sharpe, Boris Reljic, Linden Muellner-Wong, David A. Stroud, Michael T. Ryan, Megan J. Maher
Summary: COA7 is a crucial assembly factor for the biogenesis of mitochondrial complex IV. It interacts with SCO1 and SCO2 to catalyze copper relay and reduction of disulfide bonds, which are important for complex IV assembly.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Multidisciplinary
Kelsi R. Hall, Chris Joseph, Ivan Ayuso-Fernandez, Ashish Tamhankar, Lukas Rieder, Rannei Skaali, Ole Golten, Frank Neese, Asmund K. Rohr, Sergio A. V. Jannuzzi, Serena DeBeer, Vincent G. H. Eijsink, Morten Sorlie
Summary: By mutating a conserved glutamine/glutamate in Lytic polysaccharide monooxygenases (LPMOs), it was found that the nature and distance of the headgroup to the copper can fine-tune the functionality and reactivity of LPMOs. The presence of glutamate near the copper decreased the reduction potential and the ratio between reduction and reoxidation rates. These findings provide important insights for understanding LPMOs and LPMO-inspired synthetic catalysts.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Plant Sciences
You-Hui Zhong, Ze-Jun Guo, Ming-Yue Wei, Ji-Cheng Wang, Shi-Wei Song, Bing-Jie Chi, Yu-Chen Zhang, Jing-Wen Liu, Jing Li, Xue-Yi Zhu, Han-Chen Tang, Ling-Yu Song, Chao-Qun Xu, Hai-Lei Zheng
Summary: This study investigated the effect of hydrogen sulfide (H2S) on the adaptation of mangrove plant Avicennia marina to waterlogging. The results showed that waterlogging caused oxidative damage in the roots, but the application of H2S counteracted the damage. H2S reduced mitochondrial ROS production by increasing the expressions of alternative oxidase genes and altering mitochondrial respiration. In addition, H2S enhanced the antioxidant system and activated intracellular calcium sensing. This study provides evidence for the role of H2S in waterlogging adaptation in mangrove plants from a mitochondrial perspective.
PLANT CELL AND ENVIRONMENT
(2023)
