Journal
ANALYST
Volume 140, Issue 13, Pages 4393-4398Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c5an00545k
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Funding
- Ministry of Education, Culture, Sports, Science and Technology (MEXT) Supported Program for the Strategic Research Foundation at Private Universities, Japan [2014-2019]
- Hirao Taro Foundation of KONAN GAKUEN for Academic Research
- Hyogo Science and Technology Association
- Grants-in-Aid for Scientific Research [26810094] Funding Source: KAKEN
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The thermodynamic stability of certain mismatched base pairs has made the development of DNA sequence sensing systems challenging. Thus, the stability of fully matched and mismatched DNA oligo-nucleotides in the hydrated ionic liquid choline dihydrogen phosphate (choline dhp) was investigated. Mismatched base pairs were significantly destabilized in choline dhp relative to those in aqueous buffer. A molecular beacon that forms a triplex with a conserved HIV-1 sequence was then designed and tested in choline dhp. The molecular beacon specifically detected the target duplex via triplex formation at concentrations as low as 1 pmol per 10 mu L with 10000-fold sequence selectivity. Moreover, the molecular beacon was protected from a contaminating nuclease in choline dhp, and DNAs in aqueous solutions were not sufficiently stable for practical use.
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