Article
Multidisciplinary Sciences
Siyun Chen, Tamar Getter, David Salom, Di Wu, Daniel Quetschlich, Dror S. Chorev, Krzysztof Palczewski, Carol V. Robinson
Summary: This study investigated the signalling events of G protein-coupled receptors (GPCRs) using mass spectrometry in native membranes. The findings revealed the role of native lipids in rhodopsin signalling and regeneration, and proposed a paradigm for GPCR drug discovery in native membrane environments.
Article
Chemistry, Analytical
Evolene Desligniere, Simon Ollivier, Anthony Ehkirch, Armelle Martelet, David Ropartz, Nelly Lechat, Oscar Hernandez-Alba, Jean-Michel Menet, Severine Clavier, Helene Rogniaux, Bruno Genet, Sarah Cianferani
Summary: This study characterizes a trispecific antibody and uses advanced separation and mass spectrometry methods to confirm the presence of two isomeric forms and gain insights into their conformational landscape. The results highlight the benefits of combining ion mobility and mass spectrometry approaches to address analytical challenges in biopharmaceutical research.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Evolene Desligniere, Simon Ollivier, Anthony Ehkirch, Armelle Martelet, David Ropartz, Nelly Lechat, Oscar Hernandez-Alba, Jean-Michel Menet, Severine Clavier, Helene Rogniaux, Bruno Genet, Sarah Cianferani
Summary: This study characterizes a trispecific antibody with two distinct isomeric forms, utilizing advanced mass spectrometry and ion mobility techniques to gain insights into the conformational landscape of the antibody.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Naifu Zhang, Xiaohe Yu, Xinchao Zhang, Sheena D'Arcy
Summary: Hydrogen-Deuterium eXchange coupled to mass spectrometry is a powerful tool for protein dynamics and interaction analysis. Bottom-up experiments are common for observing deuterium uptake differences, producing multi-dimensional data that can be challenging to visually depict. The HD-eXplosion application fills a gap in available software, generating customizable, publication-quality plots with statistical filters.
Article
Chemistry, Medicinal
E. Sook Yen, Yunli Hu, Rosalynn Molden, Haibo Qiu, Ning Li
Summary: Monitoring and quantifying low abundance host cell proteins (HCPs) in therapeutic proteins is crucial for ensuring product quality and safety. A practical and effective strategy for identifying and quantifying problematic HCPs at low levels has been developed, facilitating downstream purification process improvement. This workflow can be easily adapted to detect and quantify other problematic HCPs at extremely low levels in therapeutic protein drug candidates.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Chemistry, Analytical
Hugo Jaegler, Alkiviadis Gourgiotis
Summary: The uranium isotope composition in environmental samples can be used to trace its origin. Researchers in the analytical community have been working to improve the detection limit of the U-236/U-238 isotopic ratio using mass spectrometry, specifically the ICP-MS/MS technology. The latest generation of ICP-MS/MS has shown improved performance and can accurately measure U-236/U-238 isotope ratios as low as 10(-11), which is a significant advancement and has various geochemical applications.
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
(2023)
Editorial Material
Chemistry, Medicinal
Anders Lund, Da Ren, Richard S. Rogers, Jason C. Rouse, X. Christopher Yu, John F. Valliere-Douglass
Summary: This commentary provides a high-level introduction to LC-MS product characterization methodologies in biopharmaceutical development, addressing areas of ambiguity in protein primary sequence confirmation and sequence variant analysis. Discussions and outcomes from recent workshops on the application of LC-MS to primary structure confirmation and sequence variant analysis are highlighted, with recommendations falling into consolidation of opinions on definitions and development of fit-for-purpose strategies in SVA experiments.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Endocrinology & Metabolism
Rossella Mazzilli, Martina Curto, Donatella De Bernardini, Soraya Olana, Matilde Capi, Gerardo Salerno, Fabiola Cipolla, Virginia Zamponi, Daniele Santi, Fernando Mazzilli, Maurizio Simmaco, Luana Lionetto
Summary: This observational study aimed to develop a new LC-MS/MS method for TDM of psychotropic drugs in seminal fluid. The results showed that seminal levels of certain psychotropic drugs were detectable and correlated with alterations in sperm concentration and motility, especially in patients treated with antipsychotics. Further studies are needed to validate these findings and explore the potential impact of semen TDM in managing male fertility issues related to psychotropic medications.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Review
Chemistry, Analytical
Rui Su, Tongtong Yang, Xinrui Zhang, Na Li, Xingying Zhai, Huanwen Chen
Summary: Breath analysis is an attractive and non-invasive strategy for monitoring metabolic dynamics in living organisms. Mass spectrometry is a powerful analytical platform for breath analysis, and ambient mass spectrometry has been widely adopted due to its minimal/sample pretreatment requirements. This tutorial review introduces the principles, setup, procedures, applications, and future prospects of mass spectrometry-based techniques for breath analysis, aiming to facilitate further development and application in this field.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Ian Oesterle, Manuel Pristner, Sabrina Berger, Mingxun Wang, Vinicius Verri Hernandes, Annette Rompel, Benedikt Warth
Summary: Polyphenols, abundant in plants and fungi, have beneficial bioactive properties. High-resolution mass spectrometry (HRMS) is favored for analyzing their complexity. This study evaluated the advantages of HRMS, using untargeted techniques and online resources, for annotating polyphenols and other molecules. Despite the sensitivity loss compared to low-resolution mass spectrometry (LRMS), HRMS is capable of comprehensively investigating human polyphenol exposure, linking it to human health effects and toxicological mixture effects with other xenobiotics.
ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Ali Khorramifar, Mansour Rasekh, Hamed Karami, Jesus Lozano, Marek Gancarz, Ewa Lazuka, Grzegorz Lagod
Summary: The activities of enzymes such as alpha-amylase, beta-amylase, sucrose synthase, and invertase are influenced by storage conditions, affecting the starch structure and sugar content of potatoes, thus impacting their quality. Storage in a warehouse is a common and effective method to maintain the freshness and sweetness of potatoes after harvest. Smart monitoring and evaluation of potato quality during storage can improve freshness.
Review
Medical Laboratory Technology
Stephanie Rankin-Turner, Liam M. Heaney
Summary: Mass spectrometry (MS) has become a powerful and versatile tool in clinical laboratory, with advancements in biomarker detection, protein measurement, therapeutic drug monitoring, and automation leading to potential revolution in clinical testing.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Review
Medical Laboratory Technology
Philippe Massonnet, Elodie Grifnee, Jordi Farre-Segura, Justine Demeuse, Loreen Huyghebaert, Thomas Dubrowski, Patrice Dufour, Matthieu Schoumacher, Stephanie Peeters, Caroline Le Goff, Etienne Cavalier
Summary: Immunocapture is a mature method for sample preparation in quantitation of peptides and proteins. This review provides an overview of clinical applications of immunocapture methods, including protocols with and without enzymatic digestion. The advantages and limitations of these approaches are discussed, along with challenges related to mass spectrometer selection. The potential of these techniques in proteomics-based clinical medicine is demonstrated, paving the way for personalized medicine.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Chemistry, Analytical
Alexandre Goyon, Daniel Nguyen, Sara Boulanouar, Peter Yehl, Kelly Zhang
Summary: The study developed a workflow to investigate the degradation of gRNA at the single nucleotide level, identifying residues prone to degradation. Analysis of stressed gRNA samples revealed that desulfurization of phosphorothioate linkages was the major degradation observed.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Runmin Yang, Jingjing Ma, Shu Zhang, Yu Zheng, Lusheng Wang, Daming Zhu
Summary: This study introduces a new storage format called mzMD for MS datasets and presents an algorithm to query this format for summarizing a given data window. Experimental results demonstrate the high speed and stability of mzMD in retrieving high-quality data window summaries.
Article
Biochemistry & Molecular Biology
Joshua A. Jadwin, Timothy G. Curran, Adam T. Lafontaine, Forest M. White, Bruce J. Mayer
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Article
Oncology
Hui Liu, Charles J. Murphy, Florian A. Karreth, Kristina B. Emdal, Kangkang Yang, Forest M. White, Olivier Elemento, Alex Toker, Gerburg M. Wulf, Lewis C. Cantley
Article
Biochemistry & Molecular Biology
Martin Schwill, Rastislav Tamaskovic, Aaron S. Gajadhar, Florian Kast, Forest M. White, Andreas Pluckthun
Article
Multidisciplinary Sciences
Elizabeth C. Randall, Kristina B. Emdal, Janice K. Laramy, Minjee Kim, Alison Roos, David Calligaris, Michael S. Regan, Shiv K. Gupta, Ann C. Mladek, Brett L. Carlson, Aaron J. Johnson, Fa-Ke Lu, X. Sunney Xie, Brian A. Joughin, Raven J. Reddy, Sen Peng, Walid M. Abdelmoula, Pamela R. Jackson, Aarti Kolluri, Katherine A. Kellersberger, Jeffrey N. Agar, Douglas A. Lauffenburger, Kristin R. Swanson, Nhan L. Tran, William F. Elmquist, Forest M. White, Jann N. Sarkaria, Nathalie Y. R. Agar
NATURE COMMUNICATIONS
(2018)
Article
Cell Biology
Louis S. Prahl, Patrick F. Bangasser, Lauren E. Stopfer, Mahya Hemmat, Forest M. White, Steven S. Rosenfeld, David J. Odde
Article
Biochemistry & Molecular Biology
Antje Dittmann, Norman J. Kennedy, Nina L. Soltero, Nader Morshed, Miyeko D. Mana, Omer H. Yilmaz, Roger J. Davis, Forest M. White
MOLECULAR SYSTEMS BIOLOGY
(2019)
Article
Multidisciplinary Sciences
Lauren E. Stopfer, Joshua M. Mesfin, Brian A. Joughin, Douglas A. Lauffenburger, Forest M. White
NATURE COMMUNICATIONS
(2020)
Article
Multidisciplinary Sciences
Ritu Raman, Erin B. Rousseau, Michael Wade, Allison Tong, Max J. Cotler, Jenevieve Kuang, Alejandro Aponte Lugo, Elizabeth Zhang, Ann M. Graybiel, Forest M. White, Robert Langer, Michael J. Cima
Article
Multidisciplinary Sciences
Walid M. Abdelmoula, Begona Gimenez-Cassina Lopez, Elizabeth C. Randall, Tina Kapur, Jann N. Sarkaria, Forest M. White, Jeffrey N. Agar, William M. Wells, Nathalie Y. R. Agar
Summary: This study demonstrates the application of artificial intelligence in mining Mass Spectrometry Imaging (MSI) data to reveal biologically relevant metabolomic and proteomic information acquired from different mass spectrometry platforms. By utilizing a fully connected variational autoencoder, the researchers developed the msiPL method to learn and visualize the underlying non-linear spectral manifold in MSI data, uncovering biologically relevant clusters of tissue anatomy and tumor heterogeneity in various tissue types.
NATURE COMMUNICATIONS
(2021)
Article
Oncology
Ishwar N. Kohale, Jia Yu, Yongxian Zhuang, Xiaoyang Fan, Raven J. Reddy, Jason Sinnwell, Krishna R. Kalari, Judy C. Boughey, Jodi M. Carter, Matthew P. Goetz, Liewei Wang, Forest M. White
Summary: Breast cancer is the leading cause of cancer-related deaths in women globally, with triple negative breast cancer (TNBC) known for being highly aggressive and resistant to chemotherapy. In this study, the researchers identified Src family kinases (SFKs) as potential drug targets for chemotherapy-resistant TNBC. However, the prevalence of SFK-driven tumors was found to be low in human tumor tissue specimens. These findings highlight the importance of characterizing phosphotyrosine signaling profiles for patient stratification and therapeutic options.
Article
Multidisciplinary Sciences
Lauren E. Stopfer, Nicholas J. Rettko, Owen Leddy, Joshua M. Mesfin, Eric Brown, Shannon Winski, Bryan Bryson, James A. Wells, Forest M. White
Summary: Combining multiple therapeutic strategies may improve survival benefits for NRAS/BRAF mutant melanoma patients. This study utilized immunopeptidomics to measure the impact of MEK inhibition on anti-tumor immunity and identified enhanced expression of specific tumor antigens following therapy. Targeting these upregulated tumor antigens can enhance cell killing.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biology
Owen Leddy, Forest M. White, Bryan D. Bryson
Article
Biology
Jacqueline S. Gerritsen, Joseph S. Faraguna, Rudy Bonavia, Frank B. Furnari, Forest M. White
Summary: This study investigated the relationship between specific EGFR phosphorylation sites and the signaling network as well as cell response. The results showed that the EGFR network remains resilient even with multiple Y-to-F mutations in the C-terminal tail. Additionally, the study revealed previously unidentified network nodes associated with EGFR signaling. The data-driven model highlighted the signaling network nodes associated with distinct EGF-driven cell responses.
LIFE SCIENCE ALLIANCE
(2023)
Article
Cell Biology
Nader Morshed, Meelim J. Lee, Felicia H. Rodriguez, Douglas A. Lauffenburger, Diego Mastroeni, Forest M. White
Summary: The study analyzed the proteome and phosphoproteome of brain tissue from Alzheimer's disease patients, uncovering correlations between neuronal synaptic protein abundances, microglial reactivity markers, and phosphorylation sites on kinases targeting tau. This provides insight into pathology-associated phosphorylation signaling events in Alzheimer's disease.
Article
Biology
Brian C. Del Rosario, Andrea J. Kriz, Amanda M. Del Rosario, Anthony Anselmo, Christopher J. Frye, Forest M. White, Ruslan Sadreyev, Jeannie T. Lee