Article
Fisheries
Vitaliy Kholodnyy, Kiko Shibata, Atife Tuba Beken, Borys Dzyuba, Hermes Bloomfield-Gadelha, Jacky Cosson, Sergii Boryshpolets
Summary: During evolution, fish have adopted diverse spawning tactics to effectively utilize sperm competition and prevent the fertilization of inappropriate male gametes. This study observed that common carps exhibit different spawning behavior compared to salmonids, and the presence of ovarian fluid (OF) altered the motility pattern and velocity of carp spermatozoa. The chemotactic test showed that carp spermatozoa responded significantly to OF, and this attraction phenomenon depended on the presence of external calcium ions. These findings highlight the significant impact of environmental conditions, particularly the presence of OF, on the performance of male gametes in common carp, aligning with their specific spawning behavior.
Article
Biochemistry & Molecular Biology
Kogiku Shiba, Kazuo Inaba
Summary: Spermatozoa can sense and respond to environmental signals to ensure successful fertilization. In the ascidian Ciona intestinalis, CNG channels play a vital role in regulating sperm motility and intracellular calcium regulation during chemotaxis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Vitaliy Kholodnyy, Borys Dzyuba, Marek Rodina, Hermes Bloomfield-Gadelha, Manabu Yoshida, Jacky Cosson, Sergii Boryshpolets
Summary: The fertilization of freshwater fish can be negatively affected by environmental factors, with egg and ovarian fluid playing a significant role in triggering gamete encounters. The ovarian fluid of rainbow trout affects sperm performance by inducing a chemotaxis-like effect on activated male gametes, creating an optimal environment for sperm performance. The individual interaction between egg (ovarian fluid) and sperm reflects specific features of the spawning process in this species.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Shengnan Cong, Jingjing Zhang, Feng Pan, Lianjun Pan, Aixia Zhang, Jiehua Ma
Summary: Ion channels in the sperm tail, such as CatSper and KSper, play a crucial role in triggering essential physiological reactions necessary for successful fertilization, like progressive motility and hyperactivation. Other ion channels like Hv1, sNHE, ENaC, TRP channels, and CFTR are also found in the flagellum. This review discusses the recent advancements in these ion channels' understanding in the flagellum, their impact on sperm function, and the interplay between them through factors like PH, membrane potential, and cAMP. These ion channels may have potential clinical applications in treating infertility.
Article
Cell Biology
Juan J. Ferreira, Pascale Lybaert, Lis C. Puga-Molina, Celia M. Santi
Summary: Our study shows that CatSper channels in mouse and human sperm are activated through alkaline conditions and the presence of HCO3-, leading to an influx of Ca2+. Furthermore, HCO3- activates the soluble adenylyl cyclase/protein kinase A pathway, which sensitizes CatSper channels to respond to membrane depolarization, allowing for Ca2+ influx.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Fisheries
Adele Fabbrocini, Fausto Silvestri, Raffaele D'Adamo
Summary: The aim of this study was to optimize the post-collection storage procedures for S. granularis semen and evaluate the effects of storage conditions on sperm motility parameters. The results showed that the motility parameters remained unchanged for up to 60 minutes after activation in both fresh and chilled semen, while cryopreserved samples had slightly lower sperm motility upon activation. However, there were no differences in motility parameters compared to fresh semen when thawed semen was cold-incubated for up to 60 minutes post activation, meeting the standards required by germplasm cryobanks.
Article
Fisheries
S. Judycka, D. Zarski, M. A. Dietrich, H. Karol, P. Hliwa, M. Blazejewski, A. Ciereszko
Summary: The study focuses on an improved semen cryopreservation protocol for commercial fertilization in European perch, showing high sperm motility with a new extender and KCl supplementation. The enhanced procedure allows for handling prediluted semen and maintaining high fertilization rates for cryopreserved and fresh semen, enabling large-scale fertilization applications.
Article
Multidisciplinary Sciences
Gary M. Wessel, Yuuko Wada, Mamiko Yajima, Masato Kiyomoto
Summary: The study found that Bindin plays a critical role in fertilization in sea urchins, but its function had never been tested. Through gene editing technology, it was successfully demonstrated that Bindin is essential for the binding of sperm and egg cell surfaces.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Vitaliy Kholodnyy, Borys Dzyuba, Hermes Gadelha, Jacky Cosson, Sergii Boryshpolets
Summary: The study explored the effects of ovarian fluid and egg-released substances on sperm behavior in sterlet. Results showed that ovarian fluid inhibited sperm motility initiation, while egg-conditioned medium attracted sperm. The presence of ovarian fluid preserved the fertilizing ability of eggs and extended the period of sperm fertilization.
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Cell Biology
Veronica Loyo-Celis, Gerardo Orta, Carmen Beltran, Alberto Darszon
Summary: Speract released from egg jelly regulates sea urchin sperm swimming through inducing chemotaxis and triggering changes in membrane potential, cyclic nucleotides, pH, and calcium concentration. CatSper, a sperm exclusive Ca2+ channel, has been identified in sea urchin sperm and is believed to be involved in chemotaxis. Electrophysiological characterization revealed a Na+ permeable channel resembling CatSper in sea urchin sperm, sensitive to speract and blocked by specific inhibitors.
Article
Multidisciplinary Sciences
Amanda B. Gillis, Emmet L. Guy, Andrew J. Kouba, Peter J. Allen, Ruth M. Marcec-Greaves, Carrie K. Kouba
Summary: This study aimed to characterize tiger salamander sperm motility and determine the effect of temperature on it. Results showed that sperm stored at 0 degrees Celsius had higher motility initially but decreased rapidly over time. There were differences in motility between milt and spermic urine, and near freezing temperatures were beneficial for sperm longevity.
Article
Biochemistry & Molecular Biology
Masahiro Naruse, Midori Matsumoto
Summary: This study elucidated the molecular mechanisms and biophysical properties of sperm chemotaxis in echinoderms. By reconstructing a sperm model in silico, the importance of desensitizing the chemoattractant receptor in long-range chemotaxis was demonstrated. Simulation results showed that desensitization is essential for sperm to approach distantly located eggs. By comparing the model with starfish sperm, the different desensitization schemes and responses to disturbances were analyzed, highlighting the potential application of this approach as a novel tool in chemotaxis research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Won-Ki Pang, Yoo-Jin Park, Myung-Geol Pang
Summary: This study investigated the potential of sperm RNA markers in assessing various aspects of sperm function and identified genes related to male fertility. The findings provide valuable insights into male infertility.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Andrology
Yiling Zhou, Yaling Wang, Jingwen Chen, Bangguo Wu, Shuyan Tang, Feng Zhang, Chunyu Liu, Lingbo Wang
Summary: This study uncovers the role of the Dnali1 gene in sperm motility and male fertility in mice. It also demonstrates that ICSI can overcome male infertility associated with Dnali1 mutations, providing guidance for the diagnosis and genetic counseling of DNALI1-associated human infertility.
BASIC AND CLINICAL ANDROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Fanwen Zeng, Mingming Wang, Ju Li, Chengde Li, Xueqing Pan, Li Meng, Li Li, Hengxi Wei, Shouquan Zhang
Summary: In this study, we found that porcine beta-defensin 129 (pBD129) is widely present in accessory sex glands and coats the surface of sperm. Inhibiting pBD129 on the sperm surface resulted in increased acrosome integrity, tyrosine phosphorylation levels, and Ca2+ influx, leading to a reduced in vitro fertilization cleavage rate. Furthermore, treatment with recombinant pBD129 restored sperm motility in semen contaminated with Escherichia coli.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemical Research Methods
R. Pinto-Camara, A. Linares, D. S. Moreno-Gutierrez, H. O. Hernandez, J. D. Martinez-Reyes, J. M. Rendon-Mancha, C. D. Wood, A. Guerrero
Summary: FCSlib is an open-source R tool specially designed for analyzing fluorescence fluctuation spectroscopy data, covering various techniques.
Article
Microbiology
Samira Lopez-Molina, Nathaly Alexandre do Nascimento, Maria Helena Neves Lobo Silva-Filha, Adan Guerrero, Jorge Sanchez, Sabino Pacheco, Sarjeet S. Gill, Mario Soberon, Alejandra Bravo
Summary: This study elucidates the mechanism of interaction between Cyt1Aa and Cry11Aa in mosquitos, as well as their behavior and effects in the gut. Cyt1Aa exerts its activity on the plasma membrane, while Cry11Aa toxin may involve additional intracellular effectors.
Article
Developmental Biology
Fang Yang, Maria Gracia Gervasi, Gerardo Orta, Darya A. Tourzani, Jose Luis De La Vega-Beltran, Gordon Ruthel, Alberto Darszon, Pablo E. Visconti, P. Jeremy Wang
Summary: The CatSper cation channel is essential for sperm capacitation and male fertility. The study identifies C2CD6 as a subunit of the CatSper complex and demonstrates its role in the formation of calcium signaling nanodomains on flagellar membranes. Deficiency of C2CD6 leads to male sterility due to depleted CatSper nanodomains and impaired sperm function. C2CD6 may facilitate the incorporation of CatSper complex into the flagellar plasma membrane and function as a calcium sensor.
Article
Cell Biology
Arlet Loza-Huerta, Hiram Pacheco-Castillo, Alberto Darszon, Carmen Beltran
Summary: Sperm motility in sea urchins is regulated by the interaction of protein kinase A and protein kinase C in the signaling pathway.
Review
Cell Biology
Carlos Bastian, Alicia Sampieri, Manuel Ortinez Benavides, Adan Guerrero, Luis Vaca
Summary: The use of super-resolution microscopy techniques has revealed a close and complex relationship between cytoskeleton reorganization and SOCE, and has identified many new proteins involved in SOCE regulation. Although there are still many unanswered questions in this dynamic field, recent developments in super-resolution microscopy promise to provide new answers soon.
Article
Developmental Biology
Gabriela Carrasquel Martinez, Andres Aldana, Jorge Carneiro, Claudia Lydia Trevino, Alberto Darszon
Summary: Mammalian sperm capacitation, involving biochemical and physiological modifications, is a crucial step for successful fertilization. This study showed that the acrosome pH gradually increases during human sperm capacitation, and the V-ATPase plays a key role in regulating the acrosome acidity. These findings provide important insights into the factors influencing sperm fusion and fertilization.
MOLECULAR HUMAN REPRODUCTION
(2022)
Article
Microscopy
Alma Alva, Eduardo Brito-Alarcon, Alejandro Linares, Esley Torres-Garcia, Haydee O. Hernandez, Raul Pinto-Camara, Damian Martinez, Paul Hernandez-Herrera, Rocco D'Antuono, Christopher Wood, Adan Guerrero
Summary: Due to the wave nature of light, optical microscopy has a limitation in resolution, but fluorescence fluctuation-based super-resolution microscopy (FF-SRM) can improve spatial resolution by statistically analyzing the temporal variations of fluorescence signals. This review describes the basic concepts, principles, and operational parameters of various FF-SRM methods published to date, providing guidance for their selection.
JOURNAL OF MICROSCOPY
(2022)
Article
Oncology
Sarai G. De Leon Rodriguez, Paul Hernandez Herrera, Cristina Aguilar Flores, Vadim Perez Koldenkova, Adan Guerrero, Alejandra Mantilla, Ezequiel M. Fuentes-Panana, Christopher Wood, Laura C. Bonifaz
Summary: This study used machine learning algorithms to analyze DCs in melanoma patients, and found that cDC1s were more abundant in tumor infiltrate of disease-free patients, with an expression profile that is consistent with an activator function. Conversely, cDC1s in metastatic patients exhibited a more tolerogenic phenotype.
JOURNAL OF ONCOLOGY
(2022)
Article
Cell Biology
Gabriel Mucino-Hernandez, Pilar Sarah Acevo-Rodriguez, Sandra Cabrera-Benitez, Adan Oswaldo Guerrero, Horacio Merchant-Larios, Susana Castro-Obregon
Summary: The nuclear architecture of mammalian cells can be altered due to abnormal accumulation of nuclear proteins or genomic alterations. This study focuses on how normal healthy cells maintain genome stability, and found that basal nucleophagy contributes to genome and nuclear stability, as well as in response to DNA damage. The findings suggest that nucleophagy plays an important role in clearing nuclear buds and micronuclei, as well as targeting autophagic proteins and specific nucleolar proteins.
JOURNAL OF CELL SCIENCE
(2023)
Article
Biology
Estefania Munoz, Jorge Carneiro
Summary: Plant-microbe symbiosis has a significant impact on ecosystem behavior and dynamics, promoting plant growth and expanding the habitability range.
JOURNAL OF THEORETICAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Esley Torres-Garcia, Raul Pinto-Camara, Alejandro Linares, Damian Martinez, Victor Abonza, Eduardo Brito-Alarcon, Carlos Calcines-Cruz, Gustavo Valdes-Galindo, David Torres, Martina Jablonski, Hector H. Torres-Martinez, Jose L. Martinez, Haydee O. Hernandez, Jose P. Ocelotl-Oviedo, Yasel Garces, Marco Barchi, Rocco D'Antuono, Ana Boskovic, Joseph G. Dubrovsky, Alberto Darszon, Mariano G. Buffone, Roberto Rodriguez Morales, Juan Manuel Rendon-Mancha, Christopher D. Wood, Armando Hernandez-Garcia, Diego Krapf, Alvaro H. Crevenna, Adan Guerrero
Summary: This study presents a new super-resolution microscopy algorithm called Mean-Shift Super Resolution (MSSR), which extends the spatial resolution of single fluorescence images beyond the diffraction limit. MSSR works on various fluorophore densities and optical setups, and has denoising capabilities.
NATURE COMMUNICATIONS
(2022)
Meeting Abstract
Biophysics
David Torres-Hernandez, Sergio A. Valdes Ramirez, Alma Y. Alva Aviles, Eduardo Brito Alarcon, Jose L. De la Vega Beltra, Alberto Darszon Isrrael, Adan O. Guerrero Cardenas, Christopher D. Wood
BIOPHYSICAL JOURNAL
(2023)
Meeting Abstract
Biophysics
Alejandro Linares, Raul Pinto-Camara, David Moreno, Haydee Hernandez, Jose Martinez, Juan Rendon-Mancha, Christopher Wood, Adan Guerrero
BIOPHYSICAL JOURNAL
(2021)
