Review
Pathology
Ji-Yeon Shin, Howard J. Worman
Summary: Nuclear envelope is composed of various components and mutations in genes encoding these components can lead to laminopathies, a group of diseases that selectively affect different tissues or organs. The mechanisms underlying these diseases are still unclear and current hypotheses focus on the impact of nuclear envelope alterations on gene expression and cellular mechanics.
ANNUAL REVIEW OF PATHOLOGY-MECHANISMS OF DISEASE
(2022)
Article
Biochemical Research Methods
Li-Chun Cheng, Xi Zhang, Kanishk Abhinav, Julie A. Nguyen, Sabyasachi Baboo, Salvador Martinez-Bartolome, Tess C. Branon, Alice Y. Ting, Esther Loose, John R. Yates, Larry Gerace
Summary: By using engineered biotin ligase TurboID and quantitative proteomics, this study explored the neighborhoods of Emerin and LBR in cultured mouse embryonic fibroblasts. The analysis revealed 232 high confidence proximity partners interacting selectively with Emerin and/or LBR, with 49 shared by both.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Plant Sciences
Justin M. Brueggeman, Ian A. Windham, Andreas Nebenfuhr
Summary: Nuclear migration during growth and development is a conserved phenomenon among many eukaryotic species. In Arabidopsis, the movement of the nucleus is important for root hair growth. This study reveals the presence of an alternative actin-based mechanism that mediates rapid nuclear displacements in the absence of myosin XI-I. Additionally, microtubules work to dampen erratic nuclear movements during root hair growth, and complex interactions between cytoskeletal arrays are observed.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Review
Cell Biology
Emily C. Storey, Heidi R. Fuller
Summary: Mutations in genes encoding proteins associated with the LINC complex can cause different diseases, but the relationship between genetic mutations and clinical phenotypes is still unclear. LMNA is the only gene where mutations commonly cause distinct conditions and there is no clear genotype-phenotype correlation. These findings provide insights into the role of LINC-complex proteins in human disease and guide future gene-targeted therapies.
Review
Biochemistry & Molecular Biology
Lenka Hornikova, Katerina Brustikova, Sandra Huerfano, Jitka Forstova
Summary: The nuclear lamina is crucial for maintaining the integrity of the cell nucleus, but it poses a barrier for virus replication. Viruses have evolved mechanisms to overcome this obstacle by inducing the assembly of multiprotein complexes and exploiting cellular kinases. Additionally, viruses use nuclear actin for intranuclear particle movement.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Nutrition & Dietetics
Undurti N. Das
Summary: Juxtaglomerular cells play a crucial role in sensing and regulating blood pressure and volume by synthesizing and releasing appropriate amounts of renin in response to external physical forces, contributing to homeostasis maintenance.
Article
Oncology
Shiori Watabe, Sayaka Kobayashi, Mizuho Hatori, Yoshimi Nishijima, Naoki Inoue, Hayato Ikota, Akira Iwase, Hideaki Yokoo, Masanao Saio
Summary: The present study investigated the correlation between nuclear morphology and nuclear membrane protein expression in different subtypes of ovarian epithelial cancer. The results showed that Lamin A plays a more significant role than emerin in maintaining nuclear morphology, and different subtypes of tumors have distinct nuclear shape characteristics.
Article
Biochemistry & Molecular Biology
Diana Viegas, Catia D. Pereira, Filipa Martins, Tiago Mateus, Odete A. B. da Cruz e Silva, Maria Teresa Herdeiro, Sandra Rebelo
Summary: DM1 is a hereditary and multisystemic disease characterized by altered nuclear profile and protein levels in patient-derived cells, indicating the relevance of nuclear envelope dysfunction in the pathogenesis of the disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Alexandra G. Liddane, James M. Holaska
Summary: It is commonly recognized in the field that cancer cells exhibit changes in the size and shape of their nuclei, which can impact cell migration and metastasis. Nuclear structural changes are predicted to regulate cancer cell migration across various tumor types, and alterations in nuclear lamina proteins, such as emerin, may play a significant role in cancer progression and clinical outcomes. Understanding the factors driving these nuclear abnormalities and their functional consequences remains an area of ongoing research in the field of cancer biology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Xaviere Lornage, Martial Mallaret, Roberto Silva-Rojas, Valerie Biancalana, Diane Giovannini, Klaus Dieterich, Safaa Saker, Jean-Francois Deleuze, Bernard Wuyam, Jocelyn Laporte, Johann Bohm
Summary: The nuclear envelopathies are severe human disorders affecting skeletal muscle, heart, brain, skin, and bones, caused by disruption of the nuclear envelope (NE) structure. Mutations in the TOR1AIP1 gene can lead to different clinical presentations, with LAP1B-specific mutations causing progressive skeletal muscle phenotypes, while mutations involving loss of both LAP1B and LAP1C isoforms result in a syndromic disorder affecting multiple systems including skeletal muscle, brain, eyes, ear, skin, and bones.
Editorial Material
Biochemistry & Molecular Biology
Kei Miyamoto, Masahiko Harata
Summary: The eukaryotic nucleus not only contains chromosomes and transcriptional components, but also structural filaments like nucleoskeleton proteins that support nuclear organization and compartments. These nucleoskeleton proteins, such as lamins and actin, influence nuclear dynamics including transcriptional regulation, chromatin organization, and DNA damage responses, playing crucial roles in cellular differentiation and animal development.
JOURNAL OF BIOCHEMISTRY
(2021)
Article
Biology
Matthew H. Doran, William Lehman
Summary: Actin, a highly conserved protein, plays diverse roles in cellular processes by interacting with actin-binding proteins. The ATP-dependent cycle of myosin attachment and detachment drives muscle contraction and cellular transport. The variations in actin function are influenced by myosin isoforms and other actin-binding proteins.
Article
Cell Biology
Sampada Mishra, Daniel L. Levy
Summary: Nuclear shape influences various cellular processes and is altered in certain diseases. This study explores the factors and forces that determine nuclear shape. The findings suggest that the interaction between nuclear F-actin and Lamin A contributes to the regulation of nuclear shape, and targeting nuclear actin dynamics may be a potential approach for rescuing disease-associated defects in nuclear shape.
JOURNAL OF CELL SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Xinpeng Hu, Xiaodan Zhao, Iong Ying Loh, Jie Yan, Zhisong Wang
Summary: This study demonstrates the sustained force generation capability of an artificial molecular motor at the single-molecule level, showcasing its autonomous chemically fueled operation and self-directed walking. The results suggest a reasonably efficient chemomechanical conversion of the motor compared to biomotors, with implications for similar force-demanding applications.
Article
Oncology
Tamaki Hiroe, Shunichi Moriya, Sayaka Kobayashi, Yoshimi Nishijima, Akira Watanabe, Ken Shirabe, Hayato Ikota, Hideaki Yokoo, Masanao Saio
Summary: This study found that the nuclear laminar protein Lamin A and inner nuclear membrane protein Emerin play important roles in the development of pancreatic intraductal papillary mucinous neoplasms (IPMNs). A decreased Lamin A positive ratio leads to nuclear enlargement in adenomas, promoting their transformation into carcinomas. Lamin A expression can serve as a reliable biomarker for distinguishing between IPMC and IPMA.
PATHOLOGY & ONCOLOGY RESEARCH
(2022)
Correction
Multidisciplinary Sciences
Coen M. Adema, LaDeana W. Hillier, Catherine S. Jones, Eric S. Loker, Matty Knight, Patrick Minx, Guilherme Oliveira, Nithya Raghavan, Andrew Shedlock, Laurence Rodrigues do Amaral, Halime D. Arican-Goktas, Juliana G. Assis, Elio Hideo Baba, Olga L. Baron, Christopher J. Bayne, Utibe Bickham-Wright, Kyle K. Biggar, Michael Blouin, Bryony C. Bonning, Chris Botka, Joanna M. Bridger, Katherine M. Buckley, Sarah K. Buddenborg, Roberta Lima Caldeira, Julia Carleton, Omar S. Carvalho, Maria G. Castillo, Iain W. Chalmers, Mikkel Christensens, Sandra Clifton, Celine Cosseau, Christine Coustau, Richard M. Cripps, Yesid Cuesta-Astroz, Scott F. Cummins, Leon Di Stefano, Nathalie Dinguirard, David Duval, Scott Emrich, Cedric Feschotte, Rene Feyereisen, Peter FitzGerald, Catrina Fronick, Lucinda Fulton, Richard Galinier, Sandra G. Gava, Michael Geusz, Kathrin K. Geyer, Gloria I. Giraldo-Calderon, Matheus de Souza Gomes, Michelle A. Gordy, Benjamin Gourbal, Christoph Grunau, Patrick C. Hanington, Karl F. Hoffmann, Daniel Hughes, Judith Humphries, Daniel J. Jackson, Liana K. Jannotti-Passos, Wander de Jesus Jeremias, Susan Jobling, Bishoy Kamel, Aurelie Kapusta, Satwant Kaur, Joris M. Koene, Andrea B. Kohn, Dan Lawson, Scott P. Lawton, Di Liang, Yanin Limpanont, Sijun Liu, Anne E. Lockyer, Ty Anna L. Lovato, Fernanda Ludolf, Vince Magrini, Donald P. McManus, Monica Medina, Milind Misra, Guillaume Mitta, Gerald M. Mkoji, Michael J. Montague, Cesar Montelongo, Leonid L. Moroz, Monica C. Munoz-Torres, Umar Niazi, Leslie R. Noble, Francislon S. Oliveira, Fabiano S. Pais, Anthony T. Papenfuss, Rob Peace, Janeth J. Pena, Emmanuel A. Pila, Titouan Quelais, Brian J. Raney, Jonathan P. Rast, David Rollinson, Izinara C. Rosse, Bronwyn Rotgans, Edwin J. Routledge, Kathryn M. Ryan, Larissa L. S. Scholte, Kenneth B. Storey, Martin Swain, Jacob A. Tennessen, Chad Tomlinson, Damian L. Trujillo, Emanuela V. Volpi, Anthony J. Walker, Tianfang Wang, Ittiprasert Wannaporn, Wesley C. Warren, Xiao-Jun Wu, Timothy P. Yoshino, Mohammed Yusuf, Si-Ming Zhang, Min Zhao, Richard K. Wilson
NATURE COMMUNICATIONS
(2017)
Article
Geriatrics & Gerontology
Mehmet U. Bikkul, Craig S. Clements, Lauren S. Godwin, Martin W. Goldberg, Ian R. Kill, Joanna M. Bridger
Article
Infectious Diseases
Joanna M. Bridger, Paul J. Brindley, Matty Knight
PLOS NEGLECTED TROPICAL DISEASES
(2018)
Article
Oncology
Mehmet U. Bikkul, Richard G. A. Faragher, Gemma Worthington, Peter Meinke, Alastair R. W. Kerr, Aakila Sammy, Kumars Riyahi, Daniel Horton, Eric C. Schirmer, Michael Hubank, Ian R. Kill, Rhona M. Anderson, Predrag Slijepcevic, Evgeny Makarov, Joanna M. Bridger
GENES CHROMOSOMES & CANCER
(2019)
Review
Genetics & Heredity
Marianne P. Henry, J. Ross Hawkins, Jennifer Boyle, Joanna M. Bridger
FRONTIERS IN GENETICS
(2019)
Article
Geriatrics & Gerontology
Craig S. Clements, Mehmet U. Bikkul, Wendy Ofosu, Christopher Eskiw, David Tree, Evgeny Makarov, Ian R. Kill, Joanna M. Bridger
Article
Oncology
Concetta Federico, Temitayo Owoka, Denise Ragusa, Valentina Sturiale, Domenica Caponnetto, Claudia Giovanna Leotta, Francesca Bruno, Helen A. Foster, Silvia Rigamonti, Giovanni Giudici, Giovanni Cazzaniga, Joanna M. Bridger, Cristina Sisu, Salvatore Saccone, Sabrina Tosi
Article
Biochemistry & Molecular Biology
Zachery R. Belak, Joshua. A. Pickering, Zoe. E. Gillespie, Gerald Audette, Mark Eramian, Jennifer. A. Mitchell, Joanna. M. Bridger, Anthony Kusalik, Christopher H. Eskiw
BIOCHEMISTRY AND CELL BIOLOGY
(2020)
Review
Biochemistry & Molecular Biology
Carla Almendariz-Palacios, Zoe E. Gillespie, Matthew Janzen, Valeria Martinez, Joanna M. Bridger, Troy A. A. Harkness, Darrell D. Mousseau, Christopher H. Eskiw
Article
Multidisciplinary Sciences
Lauren S. Godwin, Joanna M. Bridger, Helen A. Foster
Summary: The DNA halo method uses high salt concentrations and nonionic detergents to generate DNA loops that remain anchored to structures within nuclei, providing increased resolution and facilitating physical mapping. This technique allows for studying genomic interactions with all the structures that the genome is anchored by, revealing gene loci, whole chromosomes, alpha satellite, telomeres, and even RNA. HALO-FISH provides insight into nuclear organization and function in normal cells and in disease progression such as with cancer.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2021)
Article
Infectious Diseases
Michael Smith, Swara H. Yadav, Olayemi Fagunloye, Nana Adjoa J. Pels, Daniel M. Horton, Nashwah M. Alsultan, Andrea Borns, Carolyn T. Cousin, Freddie H. Dixon, Victoria T. Mann, Clarence H. Lee, Paul T. Brindley, Najib H. El-Sayed, Joanna T. Bridger, Matty H. Knight
Summary: The goal to reduce schistosomiasis by controlling the snail population faces challenges due to the complex life cycle of the parasite and lack of vaccines and new drugs. The study highlights the importance of understanding the molecular basis of how schistosomes survive in snails to effectively break the parasite's life cycle.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Cell Biology
Ishita S. Mehta, Kumars Riyahi, Rita Torres Pereira, Karen J. Meaburn, Martin Figgitt, Ian R. Kill, Christopher H. Eskiw, Joanna M. Bridger
Summary: This study reveals that chromosome territory positioning is altered in primary senescent human dermal fibroblasts, and that chromosome relocation in response to stimuli is no longer possible in senescent cells. This coincides with changes in the organization and distribution of the nuclear myosin 1 beta protein within senescent HDFs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ivana Barravecchia, Chiara De Cesari, Mattia Forcato, Francesca Scebba, Olga Pyankova, Joanna M. Bridger, Helen A. Foster, Giovanni Signore, Andrea Borghini, Mariagrazia Andreassi, Massimiliano Andreazzoli, Silvio Bicciato, Mario Enrico Pe, Debora Angeloni
Summary: Microgravity and space radiation have distinct effects on human endothelial cells in space flight, triggering different molecular pathways and leading to specific changes in cell structure and function. Tailored countermeasures should be developed separately for microgravity and space radiation to protect astronauts' health effectively.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Review
Oncology
Concetta Federico, Francesca Bruno, Denise Ragusa, Craig S. Clements, Desiree Brancato, Marianne P. Henry, Joanna M. Bridger, Sabrina Tosi, Salvatore Saccone
Summary: Recent research has made significant progress in understanding the spatial organization of the genome within the cell nucleus and its implications for diseases like cancer. The spatial positioning of genes within the nucleus is closely related to gene expression, with disruptions in genome organization potentially leading to diseases like cancer. Further studies on radial gene positioning and its correlation to gene expression in cancer are needed to enhance our understanding of the biological mechanisms underlying cancer initiation and progression.
Editorial Material
Multidisciplinary Sciences
Asli Silahtaroglu, Joanna M. M. Bridger, Elissa P. P. Lei
Summary: This article discusses the highly organized spatial organization of genomes in three dimensions and its relevance to genomic and nuclear function. It also provides an overview of the research progress made in understanding the 3D genome organization using various methods and analysis pipelines.
SCIENTIFIC REPORTS
(2022)
