Journal
BIOCHEMICAL PHARMACOLOGY
Volume 86, Issue 12, Pages 1673-1687Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bcp.2013.10.004
Keywords
Lignan glycosides; DNA topoisomerase IB poison; Apoptosis; Antileishmanial agent
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Funding
- Department of Biotechnology (DBT), Government of India [BT/PR4456/MED/29/355/2012]
- Indo-Brazil project of Department of Science and Technology (DST), Government of India [DST/INT/Brazil/RPO-01/2009/2]
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Lignans are diphenyl propanoids with vast range of biological activities. The present study provides an important insight into the anti-leishmanial activities of two lignan glycosides, viz. lyoniside and saracoside. These compounds inhibit catalytic activities of topoisomerase IB (LdTopIB) of Leishmania donovani in non-competitive manner and stabilize the LdTopIB mediated cleavage complex formation both in vitro and in Leishmania promastigotes and subsequently inhibit the religation of cleaved strand. These two compounds not only poison LdTopIB but also can interact with the free enzyme LdToplB. We have also shown that lyoniside and saracoside are cytotoxic to promastigotes and intracellular amastigotes. The protein DNA complex formation leads to double strand breaks in DNA which ultimately triggers apoptosis-like cell death in the parasite. Along with their cytotoxicity towards sodium antimony gluconate (SAG) sensitive AG83 strain, their ability to kill SAG resistant GE1 strain makes these two compounds potential anti-leishmanial candidates. Not only they effectively kill L donovani amastigotes inside macrophages in vitro, lyoniside and saracoside demonstrated strong anti-leishmanial efficacies in BALB/c mice model of leishmaniasis. Treatment with these lignan glycosides produce nitric oxide and reactive oxygen species Which result in almost complete clearance of the liver and splenic parasite burden. These compounds do not inhibit human topoisomerase IB upto 200 mu M concentrations and had poor cytotoxic effect on uninfected cultured murine peritoneal macrophages upto 100 mu M concentrations. Taken together it can be concluded that these compounds can be developed into excellent therapeutic agent against deadly disease leishmaniasis. (C) 2013 Elsevier Inc. All rights reserved.
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