Article
Medicine, Research & Experimental
Ana Cristina S. Bombaca, Leonardo A. Silva, Otavio Augusto Chaves, Lorrainy S. da Silva, Juliana M. C. Barbosa, Ari M. da Silva, Aurelio B. B. Ferreira, Rubem F. S. Menna-Barreto
Summary: This study introduces a novel anti-protozoan drug N4 and its mechanism of action against Trypanosoma cruzi, showing high activity in all parasite stages and indicating it as a potential alternative treatment for Chagas disease. The results presented here may serve as a good starting point for the development of alternative treatments for Chagas disease and for understanding the mechanism of naphthoimidazoles in T. cruzi.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Chemistry, Multidisciplinary
Yujing Zhang, Xingchao Dai, Jixue Wang, Junxi Liang, Jabor Rabeah, Xia Tian, Xiaoqiang Yao, Yanbin Wang, Shaofeng Pang
Summary: This study introduces an in-situ generated Cu-I catalytic system for the oxidative N-formylation of N-heterocycles with methanol. The formation of N-formamides from acyclic amines is also demonstrated. A possible reaction mechanism and pathway are proposed. The formation of undesired tar-like products is related to the radicals .O-2(-) and .OOH in the catalytic system.
Article
Chemistry, Multidisciplinary
Li-Jun Wu, Qianru Wang, Jianping Guo, Junnian Wei, Ping Chen, Zhenfeng Xi
Summary: Through the synergy of heterogeneous and homogeneous synthetic approaches, N-containing organic compounds can be synthesized using activated N-containing species prepared from N-2 gas and suitable carbon sources. Li2CN2 was successfully prepared as the activated N-containing species and utilized as a novel synthetic synthon for constructing N-containing organic compounds. Various valuable nitrogen-containing compounds were synthesized in moderate to excellent yields using Li2CN2 under mild conditions, including N-15-labeled products with anti-cancer activity.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Immunology
Gabriela Specker, Damian Estrada, Rafael Radi, Lucia Piacenza
Summary: This study demonstrates the protective effects of Trypanosoma cruzi mitochondrial peroxiredoxin (MPX) in macrophage infections and in response to nifurtimox (NFX) toxicity. MPX content increased after NFX treatment and overexpression of MPX enhanced resistance to the drug. MPX exhibits holdase activity and its overexpression was observed in temperature-challenged parasites. These findings suggest a protective role of MPX holdase activity against NFX toxicity.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Alina E. Perrone, Mariana Pinillo, Marcela S. Rial, Marisa Fernandez, Natalia Milduberger, Carolina Gonzalez, Patricia L. Bustos, Laura E. Fichera, Susana A. Laucella, Maria Cecilia Albareda, Jacqueline Bua
Summary: This study found that Trypanosoma cruzi, the causative agent of Chagas disease, secretes a 19kDa cyclophilin called TcCyP19, which is recognized by sera from infected mice and patients. The levels of anti-TcCyP19 antibodies significantly decreased in treated mice in the acute and chronic phase of infection, as well as in chronically infected individuals after 12 months of treatment. These findings suggest that TcCyP19 could serve as an early biomarker for the efficiency of trypanocidal treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biophysics
Yung-Hsin Cheng, Shih-Jen Cheng, Hsin-Ho Chen, Wei-Chia Hsu
Summary: Research has shown that using graphene oxide hydrogel containing Wharton's jelly mesenchymal stem cells can reduce apoptosis and inflammation in myocardial infarction patients, and improve cell viability.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Immunology
Leila dos Santos Moura, Vinicius Santana Nunes, Antoniel A. S. Gomes, Ana Caroline de Castro Nascimento Sousa, Marcos R. M. Fontes, Sergio Schenkman, Nilmar Silvio Moretti
Summary: The research highlights the involvement of lysine acetylation in the oxidative stress response of T. cruzi, especially through the mitochondrial lysine deacetylase TcSir2rp3 regulating the activity of mitochondrial superoxide dismutase A (TcSODA). The study also shows that acetylation of K97 in TcSODA plays a key role in modulating enzyme activity and maintaining redox homeostasis in trypanosomatids. Additionally, the interaction between TcSir2rp3 and TcSODA contributes to the understanding of mechanisms used by T. cruzi to progress during infection.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Jaime Altcheh, Victor Sierra, Teresa Ramirez, Jimy Jose Pinto Rocha, Ulrike Grossmann, Erya Huang, Guillermo Moscatelli, Olivia Ding
Summary: This study confirms the effectiveness and safety of a pediatric formulation of nifurtimox administered in an age- and weight-adjusted regimen for 60 days to treat children with Chagas disease.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Chemistry, Multidisciplinary
Marat Meleshin, Lukas Koch, Christoph Wiedemann, Mike Schutkowski
Summary: Here we describe a mild, efficient, and epimerization-free method for synthesizing peptide-derived 2-thiazolines and 5,6-dihydro-4H-1,3-thiazines. The reaction, based on cyclodesulfhydration of N-thioacyl-2-mercaptoethylamine or N-thioacyl-3-mercaptopropylamine derivatives, can be easily performed in aqueous solutions at room temperature and is triggered by pH change. The method yields complex thiazoline or dihydrothiazine derivatives without epimerization in excellent to quantitative yields. This method was successfully applied in the total synthesis of the marine metabolite mollamide F, leading to a revision of its stereochemistry.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Microbiology
N. Falk, A. J. Berenstein, G. Moscatelli, S. Moroni, N. Gonzalez, G. Ballering, H. Freilij, J. Altcheh
Summary: A retrospective study evaluated the treatment response of a large cohort of children and adults with Chagas disease treated with nifurtimox. The results showed that nifurtimox was highly effective in treating Chagas disease, with patients having excellent treatment responses and resolution of symptoms related to acute Trypanosoma cruzi infection. Clearance of parasitemia and a decrease in T. cruzi antibodies were observed as markers of treatment response. Younger patients showed a higher rate of seroconversion, indicating a more marked treatment response.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Microbiology
A. J. Berenstein, N. Falk, G. Moscatelli, S. Moroni, N. Gonzalez, F. Garcia-Bournissen, G. Ballering, H. Freilij, J. Altcheh
Summary: The study found that adverse drug reactions (ADRs) associated with Nifurtimox treatment for Chagas disease were relatively rare and mainly mild to moderate. ADRs primarily affected the nutritional, central nervous, and digestive systems, with no significant differences between adults and children. The proportion of treatment discontinuations due to ADRs was higher in adults compared to children.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Chemistry, Physical
Jacob P. Norman, Sharon R. Neufeldt
Summary: The regiochemical outcomes of Pd-catalyzed cross-coupling reactions for most dihalogenated N-heteroarenes are well-established, but changes to the catalyst can lead to unconventional product due to different site selectivity. This Perspective surveys unusual cases controlled by ligands or catalyst speciation where divergent selectivity occurs. Some cases have established the mechanistic origin of inverted selectivity, while others remain unknown. The remaining challenges and opportunities in the field include understanding oxidative addition mechanisms, catalyst speciation, ring substituents influence, inverted selectivity for stubborn substrates, and minimizing over-reaction of di- and polyhalogenated substrates.
Review
Biochemistry & Molecular Biology
Edio Maldonado, Diego A. Rojas, Fabiola Urbina, Aldo Solari
Summary: Chagas disease is a neglected tropical disease caused by the protozoa Trypanosome cruzi, mainly affecting Latin American countries and transmitted by insects. The disease can lead to mitochondrial dysfunction in cardiomyocytes and chronic myocardial inflammation, calling for further investigation into mitochondrial biogenesis and antioxidant pathways for treatment.
Article
Chemistry, Applied
Motohiro Yasui, Naoko Tahara, Hiroshi Matsubara, Norihiko Takeda, Masafumi Ueda
Summary: This study developed a sequential gold-catalyzed reaction for synthesizing 4-aryl-3-(2H)-isoxazolones with a hydroxy group. The method allows the introduction of an aryl and hydroxy group in a single procedure, exhibiting good chemoselectivity and tolerance towards reactive functional groups.
ADVANCED SYNTHESIS & CATALYSIS
(2022)
Review
Chemistry, Physical
Elena Perez-Mayoral, Marina Godino-Ojer, Luisa M. Pastrana-Martinez, Sergio Morales-Torres, Francisco J. Maldonado-Hodar
Summary: This paper describes different synthetic strategies for catalytic synthesis of N-containing heterocycles in carbon materials, emphasizing on the great potential of the catalysts and future research directions.
Article
Biochemistry & Molecular Biology
Juan David Figueroa, Eduardo Fuentes-Lemus, Eva Dorta, Victoria Melin, Javiera Cortes-Rios, Mario Faundez, David Contreras, Ana Denicola, Beatriz Alvarez, Michael J. Davies, Camilo Lopez-Alarcon
Editorial Material
Biochemistry & Molecular Biology
Ernesto Cuevasanta, Dayana Benchoam, Gerardo Ferrer-Sueta, Ari Zeida, Ana Denicola, Beatriz Alvarez, Matias N. Moller
Article
Biochemistry & Molecular Biology
Lia M. Randall, Joaquin Dalla Rizza, Derek Parsonage, Javier Santos, Ryan A. Mehl, W. Todd Lowther, Leslie B. Poole, Ana Denicola
FREE RADICAL BIOLOGY AND MEDICINE
(2019)
Article
Biochemistry & Molecular Biology
Milagros Burgi, Paola Hernandez, Mauricio Cabrera, Hugo Cerecetto, Mercedes Gonzalez, Ricardo Kratje, Alejandro Raimondi, Marcos Oggero, Mariela Bollati-Fogolin
BIOORGANIC CHEMISTRY
(2020)
Article
Pharmacology & Pharmacy
Saira Cancela, Lucia Canclini, Gustavo Mourglia-Ettlin, Paola Hernandez, Alicia Merlino
EUROPEAN JOURNAL OF PHARMACOLOGY
(2020)
Article
Ecology
Mariano Martinez, Laura Rodriguez-Grana, Leonardo Santos, Ana Denicola, Danilo Calliari
JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Juan Jose Marizcurrena, Silvina Acosta, Lucia Canclini, Paola Hernandez, Diego Valles, Tilman Lamparter, Susana Castro-Sowinski
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Mara Carreno, Mariana Bresque, Matias R. Machado, Leonardo Santos, Rosario Duran, Dario A. Vitturi, Carlos Escande, Ana Denicola
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Editorial Material
Biophysics
Ana Denicola
Summary: The article introduces the author's honors of becoming an elected member of the Council of IUPAB and joining the Editorial Board of Biophysical Reviews in July 2021.
BIOPHYSICAL REVIEWS
(2021)
Article
Biochemistry & Molecular Biology
Sebastian F. Villar, Joaquin Dalla-Rizza, Matias N. Moller, Gerardo Ferrer-Sueta, Leonel Malacrida, David M. Jameson, Ana Denicola
Summary: Protein self-assembly is a common feature in biology, and a new method utilizing intrinsic fluorescence lifetime with phasors is presented to study protein associations. The method was successfully applied to determine the equilibrium dissociation constant (K-D) of hPrx1 and identify its oligomeric states. The phasor approach is highly sensitive and quantitative, offering a valuable tool for assessing protein oligomerization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Mariana Ingold, Victoria de la Sovera, Rosina Dapueto, Paola Hernandez, Williams Porcal, Gloria Lopez
Summary: This study focuses on the development of nitric oxide-releasing molecules for the treatment of prostate and bladder cancers. Through multicomponent reactions, a collection of compounds with antiproliferative activity against both cancer cell lines was synthesized. These compounds have potential as lead compounds for the treatment of bladder cancer.
Article
Environmental Sciences
Silvina Acosta, Lucia Canclini, Juan Jose Marizcurrena, Susana Castro-Sowinski, Paola Hernandez
Summary: The recombinant CPD-photolyase PhrAHym from the Antarctic bacterium shows a highly efficient repair effect on DNA lesions induced by UVC light in human keratinocytes. This non-cytotoxic enzyme repairs CPD-photoproducts and reduces the formation of gamma H2AX, suggesting its potential as a biotherapeutic agent against UV-induced skin cancer, photoaging, and related diseases.
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Matias N. Moller, Florencia Orrico, Sebastian F. Villar, Ana C. Lopez, Nicolas Silva, Marcel Donze, Leonor Thomson, Ana Denicola
Summary: Red blood cells (RBCs) are vulnerable to both internal and external sources of oxidants, leading to potential damage and compromised oxygen supply. Oxyhemoglobin autoxidation is the primary endogenous source of RBC oxidants, while other blood cells and endothelium can also contribute potent oxidants. However, the abundant enzymatic systems and antioxidants in RBCs help protect against damage and maintain a healthy circulatory system. Glucose metabolism plays a significant role in providing reducing power for RBC antioxidant activity, and disruptions in this process can lead to oxidative stress-related pathologies. Overall, the redox balance in RBCs is crucial for their function and is supported by a complex network of antioxidant systems.
Review
Biochemistry & Molecular Biology
Sebastian F. Villar, Gerardo Ferrer-Sueta, Ana Denicola
Summary: Peroxiredoxins (Prx) are thiol-dependent peroxidases that function as H2O2 detoxifiers and sensors, redox signaling intermediates, metabolism modulators, and chaperones. The multifaceted nature of Prx is attributed not only to their peroxidase activity, but also to specific protein-protein interactions and dynamics of Prx oligomerization. Oxidation of Prx by peroxide substrate generates sulfenic acid, which plays a crucial role in transmitting the redox signal to various protein targets. Recent research emphasizes the importance of different Prx isoforms in cellular processes underlying disease development, with potential therapeutic applications.
CURRENT OPINION IN CHEMICAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Sebastian F. Villar, Laura Corrales-Gonzalez, Belen Marquez de los Santos, Joaquin Dalla Rizza, Ari Zeida, Ana Denicola, Gerardo Ferrer-Sueta
Summary: The reduction reactions of cytosolic human peroxiredoxins (Prx) in their disulfide form by thioredoxins (Trx) were studied. The reactivity of the nucleophilic cysteine in Trx was found to be greatly affected by the formation of the Prx-Trx complex. This activation mechanism helps understand the reduction of Prx by alternative reductants involved in redox signaling.
Article
Pharmacology & Pharmacy
Paola Orlandi, Marta Banchi, Francesca Vaglini, Marco Carli, Stefano Aringhieri, Arianna Bandini, Carla Pardini, Cristina Viaggi, Michele Lai, Greta Ali, Alessandra Ottani, Eleonora Vandini, Patrizia Guidi, Margherita Bernardeschi, Veronica La Rocca, Giulio Francia, Gabriella Fontanini, Mauro Pistello, Giada Frenzilli, Daniela Giuliani, Marco Scarselli, Guido Bocci
Summary: This study investigates the role of MC4R in melanoma and the use of the selective antagonist ML in combination with vemurafenib. The results show that ML can inhibit melanoma cell proliferation and induce apoptosis through the inhibition of ERK1/2 phosphorylation and reduction of BCL-XL expression. The combination of vemurafenib and ML exhibits a synergistic effect in vitro and inhibits tumor growth in vivo without causing adverse effects.
BIOCHEMICAL PHARMACOLOGY
(2024)
Article
Pharmacology & Pharmacy
Conor J. Bloxham, Katina D. Hulme, Fabrizio Fierro, Christian Fercher, Cassandra L. Pegg, Shannon L. O'Brien, Simon R. Foster, Kirsty R. Short, Sebastian G. B. Furness, Melissa E. Reichelt, Masha Y. Niv, Walter G. Thomas
Summary: Bitter taste receptors (T2Rs) are a type of G protein-coupled receptors that allow humans to detect aversive and toxic substances. This study characterized the functional properties of previously identified T2Rs in human cardiac tissues and their naturally occurring polymorphisms. The results showed differences in signaling among different T2R variants, and revealed a potential association between the T2R50 Tyr203 variant and cardiovascular disease.
BIOCHEMICAL PHARMACOLOGY
(2024)
Article
Pharmacology & Pharmacy
Lu Chen, Huanying Shi, Wenxin Zhang, Yongjun Zhu, Haifei Chen, Zimei Wu, Huijie Qi, Jiafeng Liu, Mingkang Zhong, Xiaojin Shi, Tianxiao Wang, Qunyi Li
Summary: This study demonstrates that Carfilzomib exhibits potent anti-tumor activity against esophageal squamous cell carcinoma (ESCC) by triggering mitochondrial apoptosis and reprogramming cellular metabolism. It has been identified that activating transcription factor 3 (ATF3) plays a crucial role as a cellular target in ESCC cells treated with Carfilzomib. Overexpression of ATF3 effectively counteracts the effects of Carfilzomib on ESCC cell proliferation, apoptosis, and metabolic reprogramming. Furthermore, ATF3 mediates the anti-tumor activity of Carfilzomib, suggesting its potential as a therapeutic agent for ESCC.
BIOCHEMICAL PHARMACOLOGY
(2024)
Review
Pharmacology & Pharmacy
Xing Zhang, Xiang Li, Ran Xia, Hong-Sheng Zhang
Summary: This review summarizes recent progress on the mechanisms of ferroptosis resistance in cancer and highlights the role of redox status and metabolism. Combination therapy for ferroptosis has great potential in treating resistant malignant tumors.
BIOCHEMICAL PHARMACOLOGY
(2024)