Journal
BIOCHEMICAL PHARMACOLOGY
Volume 77, Issue 2, Pages 159-168Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bcp.2008.10.009
Keywords
Chloride channels; Cell cycle; Cell migration; CIC-3; Antisense oligonucleotide
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Funding
- National Natural Science Foundation of China [30771106, 30800435]
- Natural Science Foundation of Guangdong Province [07005974]
- Medical Science Research Grant of Guangdong Province [B2008086]
- Wellcome Trust [056909/299/Z]
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The activation of volume-activated chloride Cl- channels has been implicated to play important roles in modulating cell cycle and cell migration. The aim of this study was to determine whether volume-activated Cl- channels are involved in cell-cycle-dependent regulation of cell migration in HeLa cells. Using techniques including cell-cycle synchronization, transwell migration assays and the patch-clamp technique, we demonstrate in this study that both the expression of volume-activated chloride cur-rent (I-Cl,I-vol) and the potential of cell migration are cell-cycle-dependent; specifically, these events were high in G(0)/G(1) phase, low in S phase, and medium in G(2)/M phase. Moreover, the mean density of I-Cl,I-vol was positively correlated to the rate of cell migration during cell-cycle progression. Additionally, endogenous suppression of I-Cl,I-vol by transfecting cells with CIC-3 antisense oligonucleotides arrested cells in S phase and slowed cell migration. Collectively, our results suggest that volume-activated Cl- channels contribute to the cell-cycle-dependent regulation of cell migration. (C) 2008 Elsevier Inc. All rights reserved.
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