Article
Biochemistry & Molecular Biology
Jose M. Espinola-Lopez, Song Tan
Summary: Histone post-translational modifications are crucial for gene expression regulation in eukaryotes, with Gcn5 playing a key role as a histone acetyltransferase in the SAGA coactivator complex. Studies have elucidated the mechanisms of Gcn5's activities on histone substrates, as well as its interactions with Ada2, Ada3, and Sgf29 in the context of histone modification.
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2021)
Article
Biochemistry & Molecular Biology
Klaus D. Grasser, Vicente Rubio, Fredy Barneche
Summary: The Spt-Ada-GCN5-acetyltransferase (SAGA) complex plays a crucial role in chromatin modification for transcription in plants, with implications in plant development and adaptive responses. However, the molecular links between SAGA or SAGA-like complexes and chromatin dynamics during transcription in Arabidopsis and other plant species are still not well understood.
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2021)
Editorial Material
Biochemistry & Molecular Biology
Seychelle M. Vos
Summary: A new study has revealed the high-resolution cryo-EM structure of the human SAGA complex, shedding light on its role in different stages of eukaryotic transcription.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Farrah El-Saafin, Didier Devys, Steven A. Johnsen, Stephane D. Vincent, Laszlo Tora
Summary: Ubiquitin is a highly conserved protein expressed in eukaryotic cells and plays a role in post-translational modification. It can be added to proteins either in a monoubiquitination or polyubiquitination manner. Monoubiquitination acts as a signaling mark to regulate various biological processes. The cellular distribution of ubiquitin is determined by the activity of ligase enzymes and deubiquitinases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Hematology
Liliana Arede, Elena Foerner, Selinde Wind, Rashmi Kulkarni, Ana Filipa Domingues, George Giotopoulos, Svenja Kleinwaechter, Maximilian Mollenhauer-Starkl, Holly Davison, Aditya Chandru, Ryan Asby, Ralph Samarista, Shikha Gupta, Dorian Forte, Antonio Curti, Elisabeth Scheer, Brian J. P. Huntly, Laszlo Tora, Cristina Pina
Summary: Epigenetic histone modifiers play key roles in regulating cell fate decisions in normal and malignant hematopoiesis. This study focuses on KAT2A, a lysine acetyltransferase, and explores its involvement in different macromolecular complexes and their distinct molecular and cellular consequences. The study identifies the contributions of ATAC and SAGA complexes in regulating biosynthetic activity and cell type-specific programs, providing insights into the requirements of KAT2A in leukemia and erythroid lineage specification and development.
Article
Microbiology
Shun-Juan Hu, Hao Zheng, Xin-Peng Li, Zhi-Xing Li, Chao Xu, Juan Li, Jia-Hua Liu, Wen-Xiao Hu, Xian-Yan Zhao, Juan-Juan Wang, Lei Qiu
Summary: In this study, the roles of Ada2 and Ada3 in the insect-pathogenic fungus Beauveria bassiana, functionally linked to Gcn5, were characterized. Loss of Ada2 and Ada3 resulted in severe growth defects, decreased blastospore yield and conidiation capacity, and reduced pathogenicity. The existence of a yeast-like Ada3-Ada2-Gcn5 complex in this fungus was confirmed.
MICROBIOLOGY SPECTRUM
(2023)
Article
Plant Sciences
Chaoyun Xu, Jing Wang, Yueqi Zhang, Yuming Luo, Youfu Zhao, Yun Chen, Zhonghua Ma
Summary: This study uncovers a novel regulatory mechanism of a transcription factor, which recruits and interacts with the SAGA complex to activate specific gene expression in pathogenic fungi.
Article
Biochemistry & Molecular Biology
Klaudia Adamus, Cyril Reboul, Jarrod Voss, Cheng Huang, Ralf B. Schittenhelm, Sarah N. Le, Andrew M. Ellisdon, Hans Elmlund, Marion Boudes, Dominika Elmlund
Summary: SLIK is a modified version of the SAGA complex, resulting from the C-terminal truncation of the Spt7 subunit. Both SLIK and SAGA act as coactivators in RNA polymerase II transcription, perform chromatin modifications, and exhibit similar affinity for TBP. Despite differences in subunit composition, SLIK retains similar structural features as SAGA and displays comparable DNA-binding properties.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Review
Genetics & Heredity
Onyekachi E. Ononye, Michael Downey
Summary: This review summarizes the types and functions of posttranslational modifications that regulate Gcn5 in both yeast and human cells. Common themes, unanswered questions, and strategies for future research are also outlined.
Article
Biochemistry & Molecular Biology
James E. Brownell, C. David Allis
Summary: The maintenance of eukaryotic genomes within DNA-protein complexes known as chromatin is regulated by post-translational modifications of chromatin proteins. GCN5 has been identified as a histone acetyltransferase, linking it to a transcription co-activator complex in yeast, emphasizing the importance of histone modifying activity in gene regulation.
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2021)
Article
Cell Biology
Shofiul Azam, In -Su Kim, Dong-Kug Choi
Summary: This study found that overexpression of alpha-synuclein (α-S) promotes neuronal apoptosis and inhibits the activity of GCN5, resulting in the transcriptional upregulation of Bim. Co-overexpression of GCN5 reverses the toxicity of α-S. These findings support the hypothesis that α-S-mediated histone deacetylation and dopaminergic neuronal loss play a role in Parkinson's disease (PD), and suggest that therapeutic activation/homeostasis of GCN5 may benefit PD and other alpha-synucleinopathies.
Article
Biochemistry & Molecular Biology
Sara T. Haile, Sanim Rahman, James K. Fields, Benjamin C. Orsburn, Namandje N. Bumpus, Cynthia Wolberger
Summary: The SAGA complex, which includes the HAT module, is a transcriptional co-activator that modifies histones through acetylation and deubiquitination. The SWIRM domain plays a crucial role in incorporating the HAT module into the SAGA complex. Deletion of the SWIRM domain results in loss of the HAT module and decreases the deubiquitinating activity of SAGA.
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2023)
Article
Biochemistry & Molecular Biology
Brittany N. Albaugh, John M. Denu
Summary: Gcn5 is a representative member of the GNAT superfamily of proteins, transferring acyl groups from CoA to acceptor nucleophiles. Research reveals that protein acetylation can reversibly regulate the function of thousands of proteins, impacting various cellular processes.
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2021)
Article
Biochemistry & Molecular Biology
Manjit Kumar Srivastav, Neha Agarwal, Poonam Poonia, Krishnamurthy Natarajan
Summary: This study elucidates how transcriptional regulators in Candida albicans control gene expression levels under iron deprivation conditions, thereby affecting iron homeostasis. The trimeric HAP complex promotes transcription of certain genes and regulates expression levels through histone modifications to maintain cellular iron balance.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Brian D. Strahl, Scott D. Briggs
Summary: Key technological innovations have greatly advanced our understanding of how genes are regulated by epigenetic factors and histone-modifying activities, particularly through the landmark finding linking histone acetylation by Gcn5 of the SAGA complex to gene activation. Studies have shown how histone-modifying activities of SAGA are regulated by and impact other histone modifications during gene transcription, with a focus on yeast as a model system. The review also discusses the roles of cis- and trans-histone crosstalk pathways involving the histone acetyltransferase, deubiquitylase, and reader domains of SAGA, as well as remaining unanswered questions.
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2021)
Article
Biophysics
Mariann Kis, Gabor Sipka, Ferhan Ayaydin, Peter Maroti
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
(2018)
Article
Biotechnology & Applied Microbiology
Viktor Vedelek, Laszlo Bodai, Gabor Grezal, Bence Kovacs, Imre M. Boros, Barbara Laurinyecz, Rita Sinka
Article
Oncology
Ildiko Huliak, Laszlo Bodai, Matyas Czepan, David Kovacs, Aniko Szabo, Laszlo Tiszlavicz, Gyoergy Lazar, Zoltan Rakonczay, Peter Hegyi, Imre Miklos Boros, Monika Kiricsi
Article
Biotechnology & Applied Microbiology
Mohana Krishna Gopisetty, David Kovacs, Nora Igaz, Andrea Ronavari, Peter Belteky, Zsolt Razga, Viktoria Venglovecz, Balint Csoboz, Imre Miklos Boros, Zoltan Konya, Monika Kiricsi
JOURNAL OF NANOBIOTECHNOLOGY
(2019)
Article
Multidisciplinary Sciences
Hajnalka Majoros, Zsuzsanna Ujfaludi, Barbara Nikolett Borsos, Viktoria Vivien Hudacsek, Zita Nagy, Frederic Coin, Krisztina Buzas, Ilona Kovacs, Tamas Biro, Imre Miklos Boros, Tibor Pankotai
SCIENTIFIC REPORTS
(2019)
Article
Plant Sciences
Norbert Andrasi, Gabor Rigo, Laura Zsigmond, Imma Perez-Salamo, Csaba Papdi, Eva Klement, Aladar Pettko-Szandtner, Abu Imran Baba, Ferhan Ayaydin, Ramakrishna Dasari, Agnes Cseplo, Laszlo Szabados
JOURNAL OF EXPERIMENTAL BOTANY
(2019)
Article
Genetics & Heredity
Gabor Nagy, Amanda Grace Vaz, Csilla Szebenyi, Miklos Tako, Eszter J. Toth, Arpad Csernetics, Otto Bencsik, Andras Szekeres, Monika Homa, Ferhan Ayaydin, Laszlo Galgoczy, Csaba Vagvolgyi, Tamas Papp
FUNGAL GENETICS AND BIOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Dora Bernula, Peter Benko, Nikolett Kaszler, Ildiko Domonkos, Ildiko Valkai, Reka Szollosi, Gyoergyi Ferenc, Ferhan Ayaydin, Attila Feher, Katalin Gemes
PLANT CELL TISSUE AND ORGAN CULTURE
(2020)
Article
Biotechnology & Applied Microbiology
David Kovacs, Nora Igaz, Annamaria Marton, Andrea Ronavari, Peter Belteky, Laszlo Bodai, Gabriella Spengler, Laszlo Tiszlavicz, Zsolt Razga, Peter Hegyi, Csaba Vizler, Imre M. Boros, Zoltan Konya, Monika Kiricsi
JOURNAL OF NANOBIOTECHNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Laszlo Henn, Aniko Szabo, Laszlo Imre, Adam Roman, Andrea Abraham, Balazs Vedelek, Peter Nanasi, Imre M. Boros
NUCLEIC ACIDS RESEARCH
(2020)
Article
Multidisciplinary Sciences
Balazs Vedelek, Asha Kiran Maddali, Nurgul Davenova, Viktor Vedelek, Imre M. Boros
SCIENTIFIC REPORTS
(2020)
Article
Biochemistry & Molecular Biology
Paula Climent-Canto, Albert Carbonell, Srividya Tamirisa, Laszlo Henn, Salvador Perez-Montero, Imre M. Boros, Fernando Azorin
Summary: In Drosophila, the germline-specific linker histone dBigH1 is expressed in germ stem cells and cystoblasts, silenced during transit-amplifying cystocytes divisions, and then upregulated during oocyte differentiation, with post-transcriptional silencing dependent on the RNA-binding protein Brat. The switch in expression from dBigH1 to somatic dH1 during maternal-to-zygotic transition is mediated by Brat, which also plays a role in translational silencing in testes despite its absence.
Article
Biochemistry & Molecular Biology
Hajnalka Majoros, Barbara N. Borsos, Zsuzsanna Ujfaludi, Zoltan G. Pahi, Monika Morocz, Lajos Haracska, Imre Miklos Boros, Tibor Pankotai
Summary: UV-induced DNA damage response and repair processes are extensively studied, with SPB10 identified as a significantly upregulated gene following UV irradiation. While SPB10 is involved in the cellular response to UV-induced stress, it does not have an essential function in cell survival, but may play a role in delaying DNA repair in certain cell phases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Balazs Vedelek, Akos Kovacs, Imre M. Boros
Summary: DNA end protection is crucial for the long-term preservation of the genome, with vertebrates utilizing Shelterin protein complex and Drosophila relying on the Terminin complex. Through in vitro assays, it was found that Verrocchio and Modigliani subunits of Terminin contain Ob-fold and contribute to ssDNA binding, with conservation of specific amino acids playing a key role in preservation of their function.
Article
Biochemistry & Molecular Biology
Divya Teja Dondapati, Pradeep Reddy Cingaram, Ferhan Ayaydin, Antal Nyeste, Andor Kanyo, Ervin Welker, Elfrieda Fodor
Summary: The cellular prion protein and its paralog Shadoo, both glycosylphosphatidylinositol-anchored proteins highly expressed in the central nervous system, share some attributes and neuroprotective actions but also have opposing roles. They may bind to the chaperone calnexin in different types of membrane domains to aid their inherently unstable native conformation during their lifetime. Further research is needed to fully understand their exact functions.