Article
Biochemistry & Molecular Biology
Attila Andor, Mahendravarman Mohanraj, Zsuzsanna Anna Pato, Katalin Uri, Beata Biri-Kovacs, Qing Cheng, Elias S. J. Arner
Summary: TXNL1 has dual functions, acting as a supporting factor for TrxR1-driven redox activities in disulfide reduction reactions and functioning as an ATP-independent chaperone.
Article
Biochemistry & Molecular Biology
Nobutoshi Yamaguchi, Toshiro Ito
Summary: Exposure to moderately high temperatures allows plants to acquire thermotolerance by removing H3K27me3 and maintaining H3K4me3 levels, enabling them to 'remember' the heat experience. JMJs mediate the balance between H3K27me3 and H3K4me3 at the HSP21 locus during heat acclimation.
Article
Biochemistry & Molecular Biology
Natalia Hess, Simon Richter, Michael Liebthal, Karl-Josef Dietz, Angelika Mustroph
Summary: The chloroplast primary metabolism is tightly regulated to respond to various environmental conditions, including daily changes between day and night. Thioredoxin-mediated redox regulation is a crucial component in regulating plastid-localized metabolic enzymes, ensuring proper assimilation and dissimilation processes in plants.
Article
Biochemistry & Molecular Biology
Madita Knieper, Lara Vogelsang, Tim Guntelmann, Jens Spross, Harald Groeger, Andrea Viehhauser, Karl-Josef Dietz
Summary: cis-(+)-12-oxophytodienoic acid (OPDA) is a reactive oxylipin that functions as a signaling molecule and regulator by tuning enzyme activities and altering gene expression. This study investigates the reaction of OPDA with specific chloroplast and cytosolic thioredoxins (TRX) and glutaredoxins (GRX) in Arabidopsis thaliana.
Article
Biochemistry & Molecular Biology
Thualfeqar Al-Mohanna, Najmeh Nejat, Anthony A. Iannetta, Leslie M. Hicks, George Popescu, Sorina C. Popescu
Summary: Upon pathogen infection, receptors in plants trigger localized and systemic immune responses, with oscillations in the redox environment altering protein function. Arabidopsis thaliana thimet oligopeptidases TOP1 and TOP2 play crucial roles in plant defense against pathogens and oxidative stress, with increased activity under oxidative regulation contributing to immune signaling. TOP1 and TOP2 are involved in ROS-induced gene expression and can cleave immune component peptides, suggesting a role in redox-mediated signaling of local and systemic immunity.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Agronomy
Langlang Ma, Chen Wang, Yu Hu, Wei Dai, Zhenjuan Liang, Chaoying Zou, Guangtang Pan, Thomas Lubberstedt, Yaou Shen
Summary: The key gene MADS26 affecting maize seed germination was identified through GWAS and transcriptomics. The study revealed variations within MADS26 that regulate seed germination traits and showed that overexpressing MADS26 improves seed germination in Arabidopsis. Understanding the genetic control of seed germination ability in maize is important for improving maize yield.
THEORETICAL AND APPLIED GENETICS
(2022)
Article
Biotechnology & Applied Microbiology
Pramod Rathor, Tudor Borza, Ramin Bahmani, Sophia Stone, Thierry Tonon, Svetlana Yurgel, Philippe Potin, Balakrishnan Prithiviraj
Summary: In this study, the brown alga Ectocarpus sp. was found to upregulate the expression of the Esi0379_0027 gene encoding a HSP70 protein in response to abiotic stresses. Bioinformatics analysis showed that the HSP70 protein is soluble, monomeric, and conserved compared to other organisms. Localization studies revealed that the EsHSP70 protein is located in the nucleus. Transgenic Arabidopsis plants expressing EsHSP70 under both constitutive and stress inducible promoters exhibited enhanced tolerance to salt stress and upregulation of stress-responsive genes. Overall, these findings suggest that the expression of EsHSP70 improves salt stress tolerance in Arabidopsis by upregulating stress-responsive genes.
JOURNAL OF APPLIED PHYCOLOGY
(2023)
Review
Chemistry, Analytical
Zilong Song, Chengwu Fan, Jintao Zhao, Lei Wang, Dongzhu Duan, Tong Shen, Xinming Li
Summary: The modulation of numerous signaling pathways is controlled by redox regulation. Maintaining dynamic redox homeostasis is crucial for human health due to the common occurrence of altered redox status in various pathological conditions. Mammalian thioredoxin reductase (TrxR), a vital component of the thioredoxin system, plays a crucial role in supporting various physiological functions, but its dysfunction disrupts redox balance and is closely associated with the pathogenesis of multiple diseases.
Article
Biochemistry & Molecular Biology
Debaprasad Koner, Niharika Nag, Parismita Kalita, Aditya K. Padhi, Timir Tripathi, Nirmalendu Saha
Summary: The presence of thioredoxin glutathione reductase (TGR) in the facultative air-breathing catfish, Clarias magur, was identified for the first time. The TGR enzyme showed both TrxR and GR activities, contributing to the maintenance of redox homeostasis and antioxidant defense under oxidative stress induced by environmental stressors. Phylogenetic analysis revealed its relationship to fish TrxRs and distant relation to platyhelminth parasites TGRs. The characterization of C. magur TGR provides insights into its unique adaptation to environmental challenges.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Plant Sciences
Keisuke Yoshida, Toru Hisabori
Summary: Various proteins in plant chloroplasts are regulated by thiol-based redox processes for light-responsive control. A specific form of phosphofructokinase (PFK), PFK5, is a novel redox-regulated protein that is suppressed upon reduction, while also being activated through oxidization by specific Trx proteins. This redox regulation of PFK5, together with other proteins like FBPase, plays a crucial role in switching light/dark metabolism in chloroplasts.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ivan A. Bychkov, Aleksandra A. Andreeva, Natalia V. Kudryakova, Elena S. Pojidaeva, Victor V. Kusnetsov
Summary: Chloroplasts' mechanisms of adaptation to elevated temperatures are determined by gene expression of the plastid transcription apparatus. Disrupting specific genes in this apparatus leads to a decrease in resistance to oxidative stress, affecting chlorophyll content, photosynthesis level, and gene response to heat stress. Moreover, there are differences in the expression of photosynthesis genes and proteins between wild-type and mutant plants under high temperature conditions.
Article
Biochemistry & Molecular Biology
Mio Tanaka, Ryuki Takahashi, Akane Hamada, Yusuke Terai, Takahisa Ogawa, Yoshihiro Sawa, Takahiro Ishikawa, Takanori Maruta
Summary: Monodehydroascorbate reductase (MDAR) is an enzyme involved in ascorbate recycling, with different numbers and distributions of isoforms in various plant species. The physiological significance of MDARs remains poorly understood. Classifying plant MDARs into three classes and studying Arabidopsis thaliana mutants lacking isoforms provide new insights into the roles of MDARs and their cooperation with other recycling systems.
Article
Biochemistry & Molecular Biology
Napaporn Sriden, Varodom Charoensawan
Summary: Comparative transcriptomic analysis provides a comprehensive understanding of transcriptional responses to temperature changes in different plant tissues, as well as the unique regulatory functions of temperature-mediating transcription factors. The goal of this study is to develop resilient cultivars to mitigate the threats of climate change on plant diversity and food security. By analyzing publicly available datasets, the researchers identified sets of temperature-responsive genes that were common or tissue-specific. Interestingly, some known temperature-responsive genes were found to be involved in cold response as well. Furthermore, the study elucidated the distinct DNA-binding patterns of key temperature transcription factors to their target genes with different transcriptional responses.
PLANT MOLECULAR BIOLOGY
(2022)
Article
Plant Sciences
Jean-Luc Montillet, Damien Rondet, Sabine Brugiere, Patricia Henri, Dominique Rumeau, Jean-Philippe Reichheld, Yohann Coute, Nathalie Leonhardt, Pascal Rey
Summary: Stomatal movements controlled by gas exchanges play a key role in plant growth in response to environmental stimuli. Thiol-reductases are involved in signaling pathways regulating guard cell movements and stomatal functioning, but their specific mechanisms remain poorly characterized.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Multidisciplinary Sciences
Deeksha Singh, Sara Blicharz, Karolina Stefanowicz, Laura Ragni, Kornel Michalak, Agnieszka Bagniewska-Zadworna, Robert Malinowski
Summary: This article presents an optimized method for fixing and clearing galls infected by Plasmodiophora brassicae. The method combines rapid optical clearing and vibratome sectioning to detect anatomical changes and localize gene expression using fluorescent proteins. This method will be useful for studying cellular and physiological responses in other pathogen-triggered structures in plants.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Plant Sciences
Akhtar Ali, Natalia Raddatz, Jose M. Pardo, Dae-Jin Yun
Summary: Plants cope with high salinity by limiting Na+ influx, compartmentalizing ions into vacuoles, exporting excess Na+ from cells, and distributing ions between aerial and root organs. Research on the salt overly sensitive (SOS) pathway and HKT1-type transporters in different species indicates that they use similar approaches to deal with salinity stress.
PHYSIOLOGIA PLANTARUM
(2021)
Article
Biochemistry & Molecular Biology
Hyun Jin Chun, Dongwon Baek, Byung Jun Jin, Hyun Min Cho, Mi Suk Park, Su Hyeon Lee, Lack Hyeon Lim, Ye Jin Cha, Dong-Won Bae, Sun Tae Kim, Dae-Jin Yun, Min Chul Kim
Summary: Our study found 50 differentially expressed proteins in salt-adapted cells, mainly related to cell structure, including cytoskeleton and cell wall biogenesis. Gene expression analysis revealed significant induction of cytoskeleton and cell wall biogenesis-related genes in salt-adapted cells. The results suggest that microtubules play crucial roles in plant adaptation and tolerance to salt stress.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Plant Sciences
Seong Dong Wi, Eun Seon Lee, Joung Hun Park, Ho Byoung Chae, Seol Ki Paeng, Su Bin Bae, Thi Kieu Anh Phan, Woe-Yeon Kim, Dae-Jin Yun, Sang Yeol Lee
Summary: CBFs are key cold-responsive transcription factors that play pleiotropic roles in the cold acclimation, growth, and development of plants. Precise regulation of CBF activity and maintenance of a critical threshold level are essential for proper plant growth under normal conditions. Redox-dependent structural switching and functional activation of CBFs protect plants under cold stress.
Article
Biochemistry & Molecular Biology
Eun Seon Lee, Joung Hun Park, Seong Dong Wi, Ho Byoung Chae, Seol Ki Paeng, Su Bin Bae, Kieu Anh Thi Phan, Min Gab Kim, Sang-Soo Kwak, Woe-Yeon Kim, Dae-Jin Yun, Sang Yeol Lee
Summary: This study investigated the importance of fatty acid modification of Trx-h2 in enhancing plant cold tolerance under cold conditions. The lack of myristoylation in Trx-h2(G/A) resulted in its nuclear localization even under warm temperature, leading to greater protection against cold stress compared to Trx-h2. Additionally, Trx-h2(G/A) overexpressing plants exhibited earlier up-regulation of COR genes and showed greater cold tolerance than wild type and Trx-h2 overexpressing plants.
Article
Biochemistry & Molecular Biology
Chang Ho Kang, Eun Seon Lee, Ganesh M. Nawkar, Joung Hun Park, Seong Dong Wi, Su Bin Bae, Ho Byoung Chae, Seol Ki Paeng, Jong Chan Hong, Sang Yeol Lee
Summary: The key regulator of light signaling, COP1, plays a crucial role in plant endoplasmic reticulum stress response by degrading HY5 in the nucleus, facilitating the unfolded protein response activation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Kien Hong Trinh, Ulhas Sopanrao Kadam, Jinnan Song, Yuhan Cho, Chang Ho Kang, Kyun Oh Lee, Chae Oh Lim, Woo Sik Chung, Jong Chan Hong
Summary: The study successfully isolated DNA aptamers that can bind to fenitrothion through the SELEX process. The FenA2 aptamer exhibited high detection sensitivity and specificity, providing a potential label-free detection mode for real-time detection of fenitrothion. This opens up new possibilities for analytical tools in various samples.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Yutong Liu, Yunshu Bai, Ning Li, Mengting Li, Wenxin Liu, Dae-Jin Yun, Bao Liu, Zheng-Yi Xu
Summary: This study reveals that the glucose sensor HXK1 interacts with Polycomb Repressive Complex 2 subunits SWN and CLF to regulate glucose signaling in Arabidopsis thaliana. They target glucose-responsive genes and function in the same genetic pathway. HXK1 is also involved in histone modification and gene repression, and CLF and SWN affect the recruitment of HXK1 to chromatin.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Plant Sciences
Irfan Ullah Khan, Akhtar Ali, Shah Zareen, Haris Ali Khan, Chae Jin Lim, Junghoon Park, Jose M. Pardo, Dae-Jin Yun
Summary: The plant hormone abscisic acid (ABA) plays a versatile and crucial role in plant immunity along with promoting abiotic stress responses. This study reveals that NPR1 acts as a positive regulator of ABA-responsive genes and is induced transcriptionally by ABA. Additionally, NPR1 is degraded through the action of HOS15 in a proteasome-dependent manner.
Article
Biochemistry & Molecular Biology
Yutong Liu, Mengting Li, Jinlei Yu, Ao Ma, Jie Wang, Dae-Jin Yun, Zheng-Yi Xu
Summary: The salinization of irrigated land reduces agricultural productivity. A mutation in OsHKT1;5 leads to a salt stress-sensitive phenotype, caused by a decrease in transcripts. This phenotype is due to the loss of function of OsDNAJ15, which encodes a plasma membrane-localized heat shock protein 40 (Hsp40).
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Review
Plant Sciences
Ho Byoung Chae, Su Bin Bae, Seol Ki Paeng, Seong Dong Wi, Kieu Anh Thi Phan, Min Gab Kim, Woe-Yeon Kim, Dae-Jin Yun, Sang Yeol Lee
Summary: Plants have developed multilayered defense strategies to cope with reactive oxygen species and redox changes. Thiol-based redox sensors act as the central machinery in plant defense signaling, perceiving H2O2 level changes and activating downstream defense signaling. This review focuses on the molecular mechanism of thiol sensors in recognizing and responding to internal/external stresses, including cold, drought, salinity, and pathogen-resistant signaling pathways. It also introduces a novel complex system of thiol-based redox sensors operating through liquid-liquid phase separation.
Article
Biochemistry & Molecular Biology
Junghoon Park, Axel J. Giudicatti, Zein Eddin Bader, Min Kyun Han, Christian Moller, Agustin L. Arce, Zheng-Yi Xu, Seong Wook Yang, Pablo A. Manavella, Dae-Jin Yun
Summary: The HOS15-HDA9 complex in Arabidopsis is a conditional suppressor of miRNA biogenesis, particularly in response to ABA. The miRNA itself can guide this complex to MIRNA loci, showing that miRNA can act as a regulator of its own expression by establishing a negative feedback loop.
Article
Biology
Chang Ho Kang, Jae Hyeok Lee, Yeon-Ju Kim, Cha Young Kim, Soo In Lee, Jong Chan Hong, Chae Oh Lim
Summary: This study investigates the biochemical and molecular functions of the Arabidopsis thaliana BAG2 (AtBAG2) protein and its physiological role under stress conditions. The results show that AtBAG2 has molecular chaperone activity attributed to its BAG domain and this activity is essential for the heat stress response in Arabidopsis.
Article
Biochemistry & Molecular Biology
Zein Eddin Bader, Min Jae Bae, Akhtar Ali, Junghoon Park, Dongwon Baek, Dae-Jin Yun
Summary: Plants use their circadian clock to adapt to daily challenges, such as water scarcity. The circadian clock component GIGANTEA (GI) promotes the process of drought escape (DE), which accelerates flowering. GI up-regulates the gene FT, an inducer of flowering. A recent study discovered a new mechanism in which GI and the protein ENHANCED EM LEVEL collaborate to activate ABA biosynthesis during drought stress.
PLANT SIGNALING & BEHAVIOR
(2023)
Article
Biochemistry & Molecular Biology
Chae Jin Lim, Akhtar Ali, Junghoon Park, Mingzhe Shen, Ki Suk Park, Dongwon Baek, Dea-Jin Yun
Summary: Cold stress is a major environmental constraint on plant growth and productivity, requiring plants to perceive cold signals and regulate the expression of specific genes. Recent research has shown that HOS15 and PWR interact in a specific way with the genome to promote the expression of COR genes and enhance cold tolerance in plants.
PLANT SIGNALING & BEHAVIOR
(2021)
Article
Biochemistry & Molecular Biology
Ki-Deuk Bae, Tae-Young Um, Won-Tae Yang, Tae-Hyeon Park, So-Yeon Hong, Kyung-Min Kim, Young-Soo Chung, Dae-Jin Yun, Doh-Hoon Kim
Summary: The dwarf and narrow leaf genes significantly affect plant height and leaf growth in rice. The newly discovered dnl-4 mutant suggests that the DNL-4 gene regulates these traits by encoding a pfkB carbohydrate kinase protein. DNL-4 expression not only influences plant height and leaf morphology but also affects productivity in rice.
PLANT SIGNALING & BEHAVIOR
(2021)